http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Ultrasmooth, Highly Spherical Monocrystalline Gold Particles for Precision Plasmonics
Lee, You-Jin,Schade, Nicholas B.,Sun, Li,Fan, Jonathan A.,Bae, Doo Ri,Mariscal, Marcelo M.,Lee, Gaehang,Capasso, Federico,Sacanna, Stefano,Manoharan, Vinothan N.,Yi, Gi-Ra American Chemical Society 2013 ACS NANO Vol.7 No.12
<P>Ultrasmooth, highly spherical monocrystalline gold particles were prepared by a cyclic process of slow growth followed by slow chemical etching, which selectively removes edges and vertices. The etching process effectively makes the surface tension isotropic, so that spheres are favored under quasi-static conditions. It is scalable up to particle sizes of 200 nm or more. The resulting spherical crystals display uniform scattering spectra and consistent optical coupling at small separations, even showing Fano-like resonances in small clusters. The high monodispersity of the particles we demonstrate should facilitate the self-assembly of nanoparticle clusters with uniform optical resonances, which could in turn be used to fabricate optical metafluids. Narrow size distributions are required to control not only the spectral features but also the morphology and yield of clusters in certain assembly schemes.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/ancac3/2013/ancac3.2013.7.issue-12/nn404765w/production/images/medium/nn-2013-04765w_0006.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/nn404765w'>ACS Electronic Supporting Info</A></P>
Herath, H.M.L.P.B.,Wickramasinghe, P.D.S.U.,Bathige, S.D.N.K.,Jayasooriya, R.G.P.T.,Kim, Gi-Young,Park, Myoung Ae,Kim, Chul,Lee, Jehee Elsevier 2017 FISH AND SHELLFISH IMMUNOLOGY Vol.60 No.-
<P><B>Abstract</B></P> <P>Glutathione reductase (GSR) is an enzyme that catalyzes the biochemical conversion of oxidized glutathione (GSSG) into the reduced form (GSH). Since the ratio between the two forms of glutathione (GSH/GSSG) is important for the optimal function of GSH to act as an antioxidant against H<SUB>2</SUB>O<SUB>2</SUB>, the contribution of GSR as an enzymatic regulatory agent to maintain the proper ratio is essential. Abalones are marine mollusks that frequently encounter environmental factors that can trigger the overproduction of reactive oxygen species (ROS) such as H<SUB>2</SUB>O<SUB>2</SUB>. Therefore, we conducted the current study to reveal the molecular and functional properties of a GSR homolog in the disk abalone, <I>Haliotis discus discus</I>. The identified cDNA sequence (2325 bp) has a 1356 bp long open reading frame (ORF), coding for a 909 bp long amino acid sequence, which harbors a pyridine nucleotide-disulfide oxidoreductase domain (171–246 aa), a pyridine nucleotide-disulfide oxidoreductase dimerization domain, and a NAD(P)(+)-binding Rossmann fold superfamily signature domain. Four functional residues: the FAD binding site, glutathione binding site, NADPH binding motif, and assembly domain were identified to be conserved among the other species. The recombinant abalone GSR (rAbGSR) exhibited detectable activity in a standard glutathione reductase activity assay. The optimum pH and optimal temperature for the reaction were found to be 7.0 and 50 °C, respectively, while the ionic strength of the medium had no effect. The enzymatic reaction was vastly inhibited by Cu<SUP>+2</SUP> and Cd<SUP>+2</SUP> ions. A considerable effect of cellular protection was detected with a disk diffusion assay conducted with rAbGSR. Moreover, an MTT assay and flow cytometry confirmed the significance of the protective role of rAbGSR in cell function. Furthermore, <I>AbGSR</I> was found to be ubiquitously distributed in different types of abalone tissues. <I>AbGSR</I> mRNA expression was significantly upregulated in response to three immune challenges: <I>Vibrio parahaemolyticus</I>, <I>Listeria monocytogenes</I>, and lipopolysaccharide (LPS), thus indicating its possible involvement in host defense mechanisms during pathogenic infections. Taken together, the results of the current study suggest that AbGSR plays an important role in antioxidant-mediated host defense mechanisms and also provide insights into the immunological contribution of AbGSR.</P> <P><B>Highlights</B></P> <P> <UL> <LI> We identified a glutathione reductase homolog (AbGSR) from disk abalone. </LI> <LI> AbGSR resembled functionally important domain architecture of GSR family. </LI> <LI> Recombinant AbGSR confirmed its biochemical properties via enzymatic assays. </LI> <LI> First functional antioxidant properties assessment of a molluscan GSR. </LI> <LI> <I>AbGSR</I> expression was modulated upon induced pathogen stress in gill and hemocytes. </LI> </UL> </P>
대간간학회지 제6차 춘계학술대회 초록집 : 포스터 발표 ; HBs Ag 양성인 간경변증 환자에서 일시적인 혈중 alpha fetoprotein 상승의 간암 발생에 대한 의의
이상우 ( Lee Sang U ),문희석 ( Mun Hui Seog ),박기오 ( Park Gi O ),이엄석 ( Lee Eom Seog ),김선문 ( Kim Seon Mun ),서승원 ( Seo Seung Won ),양현웅 ( Yang Hyeon Ung ),성재규 ( Seong Jae Gyu ),이승민 ( Lee Seung Min ),이병석 ( Lee B 대한간학회 2000 Clinical and Molecular Hepatology(대한간학회지) Vol.6 No.1(S)
기류속도 변화에 따른 온열쾌적성 평가를 위한 HRV 분석
이낙범 ( Nak B. Lee ),배동석 ( Dong S. Bae ),임재중 ( Jae J. Im ),이기섭 ( Gi S. Lee ),금종수 ( Jong S. Kum ),이구형 ( Koo H. Lee ) 한국감성과학회 1999 춘계학술대회 Vol.1999 No.-
사회와 생활수준의 발달로 인간은 자연환경하에서 생활보다 인공적 환경하에서의 생활이 증가하게 되었다. 따라서 인간은 쾌적한 생활공간을 요구하게 되었고 이를 위한 온열환경에 대한 연구가 진행되어져 왔다. 이러한 생활공간에서의 쾌적한 온열환경에 영향을 주는 물리적 요인으로는 온도와 습도 그리고 기류 등이 있다. 또한 온열환경에 대한 인간의 감성적 측면도 쾌적한 온열환경을 결정하는데 중요한 역할을 한다. 본 연구에서는 겨울철 대류난방시 인간에게 가장 쾌적한 환경을 제시할 수 있는 기류 속도의 범위를 찾고, 이를 평가하는데 객관적인 지표를 설정하기 위해 온도에 따른 기류조건을 제시하고, HRV 분석을 통하여 인체의 자율신경계의 변화를 관찰하였다. 실험결과 18 , 21 , 24 각각의 온도에서 draft를 초래하지 않는 0.15m/sec의 기류속도일 때 가장 높은 MF/(LF+HF) 값을 보여 쾌적한 기류조건으로 나타났고 이는 쾌불쾌감신고(CSV)와 동일한 결과를 나타내었다. 이러한 심전도 신호를 통한 HRV 분석은 기류조건에 따른 온열쾌적성의 평가에도 객관적이고 신뢰성 있는 지표로 제시될 수 있을 것이다. 또한 다른 환경하에서의 감성평가 척도로도 이용될 수 있을 것이라고 생각되어진다.
Gunathilaka, G.L.B.E.,Kim, Min-Gi,Lee, Chorong,Shin, Jaehyeong,Lee, Bong-Joo,Lee, Kyeong-Jun The Korean Society of Fisheries and Aquatic Scienc 2019 Fisheries and Aquatic Sciences Vol.22 No.10
Background: Taurine is a conditional essential amino acid for fish. A study was conducted to investigate the compensating effect of supplemental taurine in diets for red seabream (Pagrus major) on impaired growth performance by fish meal (FM) replacement with soybean meal (SM) at low water temperature (14.15 ± 1.95 ℃). Methods: A FM-based diet was considered as a high FM diet and three other experimental diets were formulated to replace FM with SM by 20, 35, or 50% (HFM, SM20, SM35, or SM50, respectively) without taurine and other four diets were formulated by adding 1% taurine to the diets (HFM-T, SM20-T, SM35-T, or SM50-T, respectively). Triplicate groups of fish (108.9 ± 1.58 g/fish) were distributed into 24 polyvinyl circular tanks (215 L) with 20 fish per tank and fed one of the diets to satiation for 20 weeks. Results: Growth performance and feed utilization of red seabream were significantly improved by the dietary taurine supplementation. SM20-T and SM35-T diets increased fish growth that are comparable to HFM diet. Feed intake, feed conversion ratio, and protein efficiency ratio of fish fed SM20-T and SM35-T diets were not significantly different from those of HFM group. Dietary taurine supplementation in each FM replaced group numerically increased innate immunity of the fish. Lysozyme and superoxide dismutase activities were significantly decreased in fish fed SM35, SM50, and SM50-T diets compared to those of fish fed HFM diet while they were not significantly lower in SM20, SM20-T, SM35, and SM35-T groups. Glutathione peroxidase activity was significantly lower in fish group fed SM50 diet while SM50-T group did not significantly lower compared to that of HFM group. The relative expression level of hepatic IGF-1 mRNA was improved in fish fed taurine-supplemented diets compared to their respective SM diets. Conclusions: Growth performance and feed utilization of red seabream can be accelerated or restored by 1% taurine supplementation when they are fed high level of SM up to 35% in diets during low water temperature season.