Increased retinoic acid signaling decreases lung metastasis in salivary adenoid cystic carcinoma by inhibiting the noncanonical Notch1 pathway

Zhou Meng-jiao,Yang Jia-jie,Ma Ting-yao,Feng Ge-xuan,Wang Xue-lian,Wang Li-Yong,Ge Yu-ze,Gao Ran,Hong-liang Liu,Shan Lin,Kong Lu,Chen Xiao-hong 생화학분자생물학회 2023 Experimental and molecular medicine Vol.55 No.-

MYB-NFIB fusion and NOTCH1 mutation are common hallmark genetic events in salivary gland adenoid cystic carcinoma (SACC). However, abnormal expression of MYB and NOTCH1 is also observed in patients without MYB-NFIB fusion and NOTCH1 mutation. Here, we explore in-depth the molecular mechanisms of lung metastasis through single-cell RNA sequencing (scRNA-seq) and exome target capture sequencing in two SACC patients without MYB-NFIB fusion and NOTCH1 mutation. Twenty-five types of cells in primary and metastatic tissues were identified via Seurat clustering and categorized into four main stages ranging from near-normal to cancer-based on the abundance of each cell cluster in normal tissue. In this context, we identified the Notch signaling pathway enrichment in almost all cancer cells; RNA velocity, trajectory, and sub-clustering analyses were performed to deeply investigate cancer progenitor-like cell clusters in primary tumor-associated lung metastases, and signature genes of progenitor-like cells were enriched in the “MYC_TARGETS_V2” gene set. In vitro, we detected the NICD1-MYB-MYC complex by co-immunoprecipitation (Co-IP) and incidentally identified retinoic acid (RA) as an endogenous antagonist of genes in the “MYC_TARGETS_V2” gene set. Following this, we confirmed that all-trans retinoic acid (ATRA) suppresses the lung metastasis of SACC by correcting erroneous cell differentiation mainly caused by aberrant NOTCH1 or MYB expression. Bioinformatic, RNA-seq, and immunohistochemical (IHC) analyses of primary tissues and metastatic lung tissues from patients with SACC suggested that RA system insufficiency partially promotes lung metastasis. These findings imply the value of the RA system in diagnosis and treatment.

An Improved Interval AHP Method for Assessment of Cloud Platform-based Electrical Safety Monitoring System

Wang, Shou-Xiang,Ge, Lei-Jiao,Cai, Sheng-Xia,Zhang, Dong The Korean Institute of Electrical Engineers 2017 Journal of Electrical Engineering & Technology Vol.12 No.2

Electrical safety monitoring System (ESMS) is a critical component in modern power systems, which is characterized by large-scale access points, massive users and versatile requirements. For convenience of the information integration and analysis, the software development, maintenance, and application in the system, the cloud platform based ESMS is established and assessed in this paper. Firstly the framework of the system is proposed, and then the assessment scheme with a set of evaluation indices are presented, by which the appropriate cloud product can be chosen to meet the requirements of a specific application. Moreover, to calculate the weights of the evaluation indices under uncertainty, an improved interval AHP method is adopted to take into consideration of the fuzziness of expert scoring, the qualitative consistency test, and the two normalizations in the process of eigenvectors. Case studies have been made to verify the feasibility of the assessment approach for ESMS.

Effects of mimic of manganese superoxide dismutase on 2,4,6- trinitrobenzene sulfonic acid-induced colitis in rats

Yan-Hong Wang,Jiao Dong,Jian-Xin Zhang,Jing Zhai,Bin Ge 대한약학회 2016 Archives of Pharmacal Research Vol.39 No.9

The mimic of manganese superoxide dismutase (MnSODm) has been synthesized and reported to have antiinflammatory properties. However, whether MnSODm has anti-inflammatory effects on colitis and any underlying mechanisms are poorly understood. This study was to investigate therapeutic effects and mechanism of MnSODm on 2,4,6-trinitrobenzenesulfonic acid (TNBS) induced colitis model in rats. Rats were intragastrically administered MnSODm (10, 20, and 40 mg/kg) per day for 7 days after colitis was induced by TNBS. After treated with MnSODm, the colonic macroscopic and microscopic damage scores and colonic weight/length ratios were significantly decreased compared with colitis model group. Myeloperoxidase (MPO) activity, malonyldialdehyde (MDA), tumor necrosis factor-α (TNF-α), interleukin (IL)-1β, IL-6, and IL-8 levels in colon tissues were also significantly decreased in MnSODm treatment groups. However, superoxide dismutase (SOD) activity significantly increased and phosphorylated inhibitory kappa B-alpha (IκBα), inhibitor kappa B kinase (IKKα/β), and nuclear factor-kappa Bp65 (NF-κBp65) as well as Toll-like receptor 4 (TLR4) and myeloid differentiation actor 88 (MyD88) in the colonic mucosa were significantly inhibited by MnSODm treatment. Thus, MnSODm was protective against colitis via antioxidant activity and by inhibiting inflammatory mediators by downregulating TLR4/MyD88/NF-κB signaling pathways. These data suggest a potential therapeutic effect of MnSODm in colitis.

An Improved Interval AHP Method for Assessment of Cloud Platform-based Electrical Safety Monitoring System

Shou-Xiang Wang,Lei-Jiao Ge,Sheng-Xia Cai,Dong Zhang 대한전기학회 2017 Journal of Electrical Engineering & Technology Vol.12 No.2

Electrical safety monitoring System (ESMS) is a critical component in modern power systems, which is characterized by large-scale access points, massive users and versatile requirements. For convenience of the information integration and analysis, the software development, maintenance, and application in the system, the cloud platform based ESMS is established and assessed in this paper. Firstly the framework of the system is proposed, and then the assessment scheme with a set of evaluation indices are presented, by which the appropriate cloud product can be chosen to meet the requirements of a specific application. Moreover, to calculate the weights of the evaluation indices under uncertainty, an improved interval AHP method is adopted to take into consideration of the fuzziness of expert scoring, the qualitative consistency test, and the two normalizations in the process of eigenvectors. Case studies have been made to verify the feasibility of the assessment approach for ESMS.

Function of Global Regulator CodY in Bacillus thuringiensis BMB171 by Comparative Proteomic Analysis

( Ming Xia Qi ),( Fei Mei ),( Hui Wang ),( Ming Sun ),( Ge Jiao Wang ),( Ziniu Yu ),( Yeon Ho Je ),( Ming Shun Li ) 한국미생물 · 생명공학회 2015 Journal of microbiology and biotechnology Vol.25 No.2

CodY is a highly conserved protein in low G+C gram-positive bacteria that regulates genes involved in sporulation and stationary-phase adaptation. Bacillus thuringiensis is a grampositive bacterium that forms spores and parasporal crystals during the stationary phase. To our knowledge, the regulatory mechanism of CodY in B. thuringiensis is unknown. To study the function of CodY protein in B. thuringiensis, BMB171codY- was constructed in a BMB171 strain. A shuttle vector containing the ORF of cry1Ac10 was transformed into BMB171 and BMB171codY-, named BMB171cry1Ac and BMB171codY-cry1Ac, respectively. Some morphological and physiological changes of codY mutant BMB171codY-cry1Ac were observed. A comparative proteomic analysis was conducted for both BMB171codY-cry1Ac and BMB171cry1Ac through two-dimensional gel electrophoresis and MALDI-TOF-MS/MS analysis. The results showed that the proteins regulated by CodY are involved in microbial metabolism, including branched-chain amino acid metabolism, carbohydrate metabolism, fatty acid metabolism, and energy metabolism. Furthermore, we found CodY to be involved in sporulation, biosynthesis of poly-β-hydroxybutyrate, growth, genetic competence, and translation. According to the analysis of differentially expressed proteins, and physiological characterization of the codY mutant, we performed bacterial one-hybrid and electrophoretic mobility shift assay experiments and confirmed the direct regulation of genes by CodY, specifically those involved in metabolism of branched-chain amino acids, ribosomal recycling factor FRR, and the late competence protein ComER. Our data establish the foundation for in-depth study of the regulation of CodY in B. thuringiensis, and also offer a potential biocatalyst for functions of CodY in other bacteria.

Structure and Magnetic Properties of Cr₂O₃/CrO₂ Nanoparticles Prepared by Reactive Laser Ablation and Oxidation under High Pressure of Oxygen

P. Z. Si,X. L. Wang,X. F. Xiao,H. J. Chen,X. Y. Liu,L. Jiang,J. J. Liu,Z. W. Jiao,H. L. Ge 한국자기학회 2015 Journal of Magnetics Vol.20 No.3

Cr₂O₃ nanoparticles were prepared via one-step reactive laser ablation of Cr in oxygen. The metastable CrO₂ phase was obtained through the subsequent oxidation of Cr₂O₃ nanoparticles under O₂ with gas pressures of up to 40 MPa. The as-prepared Cr₂O₃ nanoparticles are spherical or rectangular in shape with sizes ranging from 20 nm to 50 nm. High oxygen pressure annealing is effective in producing meta-stable CrO₂ from as-dried Cr₂O₃ nanoparticles, and the Cr₂O₃ nanoparticles exhibit a weak ferromagnetic behavior with an exchange bias of up to 11 mT that can be ascribed to the interfacial exchange coupling between uncompensated surface spins and the antiferromagnetic core. The Cr₂O₃/CrO₂ nanoparticles exhibit an enhanced saturation magnetization and a reduced exchange bias with an increasing faction of CrO₂ due to the elimination of uncompensated surface spins over the Cr₂O₃ nanoparticles when exposed to a high pressure of O₂ and/or possible phase segregation that results in a smaller grain size for both Cr₂O₃ and CrO₂.