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( Correa Llanten Daniela ),( Juanita Larrain Linton ),( Patricio A Munoz ),( Miguel Castro ),( Freddy Boehmwald ),( Jenny M Blamey ) 한국미생물생명공학회(구 한국산업미생물학회) 2013 한국미생물·생명공학회지 Vol.41 No.3
A gram-positive, rod-shaped, spore-forming, motile thermophilic bacterium was isolated from a sterilization oven. The microorganism GWE1, formally named Geobacillus wiegelii identified as a member of the genus Geobacillus. GWE1 grew under aerobic conditions of between 60-80ºC (optimum 70ºC), in a pH range of 3.0-8.0 (optimum pH70ºC 5.8), and between 0 and 2 M NaCl (optimum 0.3 M). The membrane polar lipids were dominated by branched saturated fatty acids, which included as the major constituents; iso-15:0 (13.3%), 16:1(ω7) (12.8%), 16:0 (28.5%), iso-17:0 (13.5%) and anteiso-17:0 (12.3%). The DNA G+C content was 47.2 mol% (determined by HPLC). The 16S rRNA gene sequence of GWE1 showed a high similarity with Geobacillus caldoxylosilyticus (97%). However, the level of DNA?DNA relatedness was only 58%. These data suggest that GWE1 is probably a novel specie of the genus Geobacillus.