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뇌수막종 제거 시 발생한 Gasserian ganglion 형 삼차신경 심반사 - 증례보고 -
신은실 ( Eunsil Shin ),이상호 ( Sangho Lee ),최정현 ( Jeong-hyun Choi ),박성욱 ( Sung Wook Park ) 경희대학교 경희의료원 2018 慶熙醫學 Vol.33 No.1
The trigeminocardiac reflex (TCR) is defined as sudden onset of parasympathetic dysrhythmia, mean arterial blood pressure change, apnea, or gastric hypermotility during stimulation of any branches of the trigeminal nerve. Clinical presentation of TCR is varied according to three subtypes, which are central, peripheral and Gasserian ganglion type. Gasserian ganglion type is rarely reported and caused by direct stimulation around the Gasserian ganglion. A 61-year-old female with meningioma around the trigeminal nerve was scheduled for meningioma removal. Unexpected severe bradycardia and hypertension happened when the surgeon manipulate the mass around Gasserian ganglion in the Meckel's cave. Gasserian ganglion type TCR was suspected and hemodynamic changes were stabilized after discontinuation of surgical manipulation and atropine injection. After that, the patient repeatedly showed severe bradycardia every mass manipulation, and surgeon decided to end the operation after only partial mass resection. The patient was discharged on the 12th postoperative day without any complications.
The molecular mechanisms of vitamin C on cell cycle regulation in B16F10 murine melanoma
Hahm, Eunsil,Jin, Dong-Hoon,Kang, Jae Seung,Kim, Young-In,Hong, Seung-Woo,Lee, Seung Koo,Kim, Ha Na,Jung, Da Jung,Kim, Jee Eun,Shin, Dong Hoon,Hwang, Young Il,Kim, Yeong Seok,Hur, Dae Young,Yang, Yool Wiley Subscription Services, Inc., A Wiley Company 2007 Journal of cellular biochemistry Vol.102 No.4
<P>Vitamin C has inconsistent effects on malignant tumor cells, which vary from growth stimulation to apoptosis induction. It is well known that melanoma cells are more susceptible to vitamin C than any other tumor cells, but the precise mechanism remains to be elucidated. In the present study, the proliferation of B16F10 melanoma cells was suppressed by vitamin C, which induced growth arrest in a dose-dependent manner without cytotoxic effects. Therefore, we investigated the changes in cell cycle distribution of B16F10 melanoma cells by staining DNAs with propidium iodide (PI). The growth inhibition of B16F10 melanoma by vitamin C was associated with an arrest of cell cycle distribution at G1 stage. In addition, the levels of p53-p21<SUP>Waf1/Cip1</SUP> increased during G1 arrest, which were essential for vitamin C-induced cell cycle arrest. The increased p21<SUP>Waf1/Cip1</SUP> inhibited CDK2. Moreover, the activity of p53-p21<SUP>Waf1/Cip1</SUP> pathway was closely related with the activation of checkpoint kinase 2 (Chk2). Inhibitor of the PI3K-family, LY294002 and the ATM/ATR inhibitor, caffeine, blocked vitamin C-induced growth arrest in B16F10 melanoma cells. These results suggest that vitamin C might be a potent agent to inhibit proliferative activity of melanoma cells via the regulation of Chk2-p53-p21<SUP>Waf1/Cip1</SUP> pathway. J. Cell. Biochem. 102: 1002–1010, 2007. © 2007 Wiley-Liss, Inc.</P>
Lee, Hye Shin,Choi, Jinhyeok,Son, Taekwon,Lee, Eun Ji,Kim, Jeong-Gyun,Ryu, Soo Hyung,Lee, Danbi,Jang, Myoung Kuk,Yu, Eunsil,Chung, Young-Hwa,Gelman, Irwin H.,Kim, Kyu-Won D.A. Spandidos 2018 Oncology letters Vol.16 No.5
<P>AKAP12 belongs to A-kinase anchoring protein (AKAP) family of scaffold proteins and is known as a tumor suppressor in several human cancer types. Its role as a tumor suppressor in hepatocellular carcinoma (HCC) was proposed due to its downregulation and epigenetic modification in human HCC; however, the effect of its deficiency on liver injuries, such as liver fibrosis and cancer has been poorly studied. By analyzing tumor and non-tumor tissues of 15 patients with HCC, it was confirmed that AKAP12 expression was downregulated in human HCC as compared with adjacent non-tumor tissues. Immunohistochemical staining of mouse liver tissue for AKAP12 revealed that its sinusoidal expression was diminished in capillarized endothelium after 8 weeks of thioacetamide (TAA) administration. AKAP12 deficiency resulted in the promotion of ductular response of biliary epithelial cells, whereas overall fibrosis and myofibroblast activation were comparable between genotypes after short-term TAA treatment. The mRNA expressions of some fibrosis-related genes such as those encoding epithelial cell adhesion molecule, collagen type 1 α1 and elastin were upregulated in liver tissues of AKAP12-knockout mice. Long-term administration of TAA for 26 weeks led to the development of liver tumors; the incidence of tumor development was higher in AKAP12-deficient mice than in wild-type littermates. Together, these results suggest that AKAP12 functions as a tumor suppressor in liver cancer and is associated with the regulation of hepatic non-parenchymal cells.</P>
장혜진(Hyejin Jang),이은실(Eunsil Lee),심유신(You-Shin Shim),서동원(Dongwon Seo),황진봉(Jinbong Hwang),이송진(Songjin Lee),하재호(Jaeho Ha) 한국식품과학회 2013 한국식품과학회지 Vol.45 No.6
담양군 죽순으로 제조한 죽순식초와 시판 과실식초의 이화학적 특성인 산도, 무기질, 아미노산, 플라보놀을 비교 분석하고 SBSE-GC/MS방법을 사용하여 향기성분을 분석하였다. 죽순식초의 산도는 4.49%로 제일 낮았으며 무기질 중 Na의 경우 8.36mg/100 g으로 다른 과실식초에 비해 그 함량이 낮았다. 아미노산은 다른 시판 식초에 비해 tyrosine과 lysine의 함량이 높았으며 flavonol aglycone 중 quercetin이 3.29 mg/L 들어있었다. SBSE방법으로 향기성분을 분석한 결과 aldehyde류의 함량이 시판식초에 비해 높았으며 특이적으로 수면 유도물질로 알려진 oleamide가 처음으로 검출되었다. We assessed the quality of a bamboo shoot vinegar produced in Damyang-gun in terms of parameters that included acidity, mineral contents, amino acids, and flavonol aglycones. The flavor compounds of the bamboo shoot vinegar were also analyzed using the stir bar sorptive extraction (SBSE) method. The acidity of the bamboo shoot vinegar was 4.49%, which was the lowest value among the commercial vinegars studied. The bamboo shoot vinegar had a lower concentration of Na (8.36 mg/100 g) than the other commercial vinegars. There was a relatively large amount of tyrosine and lysine in the bamboo shoot vinegar. The concentration of quercetin, a flavonol aglycone, was 3.29 mg/100 g. The results of the flavor analysis showed that hexanal, 2-furancarboxaldehyde, and benzaldehyde were high in the bamboo shoot vinegar. Oleamide, a compound that is known to induce sleep, was first found in bamboo shoot vinegar using the SBSE method.
( Jeong-hyun Choi ),( Eunsil Shin ),( Jeong Eun Kim ),( Sangho Lee ),( Wonsik Ahn ) 경희대학교 경희의료원 2019 慶熙醫學 Vol.34 No.1
Background and Objectives: Preoperative electrocardiography (ECG) is performed as a screening test for heart disease. Left ventricular hypertrophy (LVH) is associated with an increased risk of cardiovascular morbidity and mortality. However, the accuracy of ECG in diagnosing LVH is controversial. Therefore, it is necessary to investigate the diagnostic accuracy of conventional ECG voltage criteria in screening for LVH in patients undergoing elective surgery. Methods: We retrospectively reviewed the electronic medical records of patients aged ≥19 years who underwent elective surgery and had both preoperative standard 12-lead resting ECG and transthoracic echocardiography. LVH was diagnosed based on the Sokolow-Lyon index. Echocardiographic diagnosis of LVH was defined as a left ventricular mass index (LVMI) > 115 g/㎡ in men and > 95 g/㎡ in women. Results: Our study included 412 patients. LVH was diagnosed in 46 patients based on ECG criteria and in 137 patients based on echocardiographically derived LVMI. The area under the curve was 0.596 (95% confidence interval 0.51-0.69, P=0.029). The sensitivity, specificity, positive and negative predictive values, and the error rate were 41.3, 67.8, 13.9, 90.2, and 35.2%, respectively. Conclusions: In conclusion, ECG-based criteria to diagnose LVH in Asian patients undergoing elective surgery showed low sensitivity and specificity, indicating low diagnostic accuracy of this modality. Further studies that could improve the diagnostic accuracy of an ECG could reduce the economic burden by avoiding unnecessary tests and enable accurate preoperative evaluation of patients.
두경부종양 세포주에서 Ku 단백질 발현 정도에 따른 방사선 민감도
이상욱(Sang-wook Lee),유은실(Eunsil Yu),이소령(So-Lyoung Yi),손세희(Se-Hee Son),김종훈(Jong Hoon Kim),안승도(Seung Do Ahn),신성수(Seong Soo Shin),최은경(Eun Kyung Choi) 대한방사선종양학회 2004 Radiation Oncology Journal Vol.22 No.3
목 적: DNA-dependent protein kinase (DNA-PK)는 serine/threonine kinase로 470 kDa의 catalytic subunit (DNA- PKcs)와 각각 70 kDa과 86 kDa의 무게를 갖는 Ku 70, Ku 80 단백질로 구성된다. 이 DNA-PK는 방사선에 의해 DNA의 두 가닥이 동시에 절단되는 경우 DNA 손상 복구에 핵심적 역할을 하는 것으로 알려져 있다. 본 연구에서 Ku 발현 과 이온화방사선에 민감도와의 상관 관계를 알아보고자 하였다. 대상 및 방법: 예비실험으로 두경두종양 기원의 세포주 9개에 대한 방사선에 대한 민감도실험을 한 결과 AMC-HN3이 방사선에 가장 민감하였고, AMC-HN9이 방사선에 가장 저항성을 보여 2개의 세포주에 대한 Ku70/80 의 발현을 Western blot과 면역형광 염색을 시행하여 방사선의 반응도와의 상관관계를 알아보았다. 결 과: 방사선에 저항성을 보이는 AMC-HN9에서 Ku80 발현이 높았고 방사선에 민감한 AMC-HN3에서 세포자멸사가 더 많이 일어남을 관찰할 수 있었다. 결 론: Ku80 단백질 발현이 높은 세포는 방사선에 대한 DNA 손상 복구가 많이 되어 방사선에 대한 내성을 보이는 것으로 생각되었다. Purpose: DNA-dependent protein kinase (DNA-PK) is a serine/threonine kinase consisting of a 470 kDa catalytic subunit (DNA-PKcs) and a heterodimeric regulatory complex, called Ku, which is composed of 70 kDa (Ku 70) and 86 kDa (Ku 80) proteins. The DNA-PK has been shown to play a pivotal role in rejoining DNA double-strand-breaks (dsb) in mammalian cells. The purpose of this study is to examine the relationship between the level of Ku expression and radiation sensitivity. Methods and Materials: Nine head and neck, cancer cell lines showed various intrinsic radiation sensitivities. Among the nine, AMC-HN-3 cell was the most sensitive for X-ray irradiation and AMC-HN-9 cell was the most resistance. The most sensitive and resistant cell lines were selected and the test sensitivity of radiation and expression of Ku were measured. Radiation sensitivity was obtained by colony forming assay and Ku protein expression using Western blot analysis. Results: Ku80 increased expression by radiation, wheres Ku70 did not. Overexpression of Ku80 protein increased radiation resistance in AMC-HN9 cell line. There was a correlation between Ku80 expression and radiation resistance. Ku80 was shown to play an important role in radiation damage response. Conclusion: Induction of Ku80 expression had an important role in DNA damage repair by radiation. Ku80 expression may be an effective predictive assay of radiosensitivity on head and neck cancer.
Kang, Hyo Jeong,Haq, Farhan,Sung, Chang Ohk,Choi, Jene,Hong, Seung-Mo,Eo, Soo-Heang,Jeong, Hui Jeong,Shin, Jinho,Shim, Ju Hyun,Lee, Han Chu,An, Jihyun,Kim, Mi-Ju,Kim, Kyu-pyo,Ahn, Sung-Min,Yu, Eunsil S. Karger AG 2019 Liver cancer Vol.8 No.1
<P><B><I>Background:</I></B> <I>FGF19</I> amplification is a relatively novel type of genetic aberration that has been proposed to be a driver of hepatocarcinogenesis. Selective inhibitors of <I>FGFR4</I>, a receptor of <I>FGF19</I>, have been developed as targeted therapies for hepatocellular carcinoma (HCC). Despite the role of <I>FGF19</I> in mediating HCC progression, the clinicopathological characterization of patients exhibiting <I>FGF19</I> amplification remains unclear. Immunohistochemical staining is the simplest and most widely used method of identifying aberrations in the <I>FGF19</I> gene, although its specificity is very low. <B><I>Methods:</I></B> This study investigated the prognostic significance of <I>FGF19</I> amplification in a large cohort of 989 HCC patients using fluorescence in situ hybridization (FISH), which has a high degree of specificity. In addition, FISH data from formalin-fixed, paraffin-embedded sections were compared with copy number variation (CNV) data obtained from fresh frozen sections to validate the use of FISH as a diagnostic tool. <B><I>Results:</I></B> <I>FGF19</I> amplifications were detected by FISH in 51 (5.15%) of the 989 patients, and were independently associated with poor survival and a higher risk of tumor recurrence, as well as with poor prognostic factors such as a high α-fetoprotein level, hepatitis B or C virus infection, a large tumor size, microvascular invasion, and necrosis. In addition, <I>FGF19</I> amplification was associated with <I>TP53</I> mutation, and was mutually exclusive with <I>CTNNB1</I> mutation. The results of the FISH and CNV analyses exhibited a significant concordance rate of 96% (κ = 0.618, <I>p</I> < 0.001). <B><I>Conclusions:</I></B> These data indicate that <I>FGF19</I> amplification represents a unique molecular subtype associated with poor prognostic characteristics, which supports the hypothesis that the <I>FGF19-FGFR4</I> signaling pathway plays an important role in hepatocarcinogenesis. We have also demonstrated that FISH is a viable alternative to CNV analysis, offering a number of advantages in the clinical setting.</P>