Sodium butyrate has context-dependent actions on dipeptidyl peptidase-4 and other metabolic parameters

Lee, Eun-Sol,Lee, Dong-Sung,Pandeya, Prakash Raj,Kim, Youn-Chul,Kang, Dae-Gil,Lee, Ho-Sub,Oh, Byung-Chul,Lee, Dae Ho The Korean Society of Pharmacology 2017 The Korean Journal of Physiology & Pharmacology Vol.21 No.5

Sodium butyrate (SB) has various metabolic actions. However, its effect on dipeptidyl peptidase 4 (DPP-4) needs to be studied further. We aimed to evaluate the metabolic actions of SB, considering its physiologically relevant concentration. We evaluated the effect of SB on regulation of DPP-4 and its other metabolic actions, both in vitro (HepG2 cells and mouse mesangial cells) and in vivo (high fat diet [HFD]-induced obese mice). Ten-week HFD-induced obese C57BL/6J mice were subjected to SB treatment by adding SB to HFD which was maintained for an additional 16 weeks. In HepG2 cells, SB suppressed DPP-4 activity and expression at sub-molar concentrations, whereas it increased DPP-4 activity at a concentration of $1,000{\mu}M$. In HFD-induced obese mice, SB decreased blood glucose, serum levels of insulin and $IL-1{\beta}$, and DPP-4 activity, and suppressed the increase in body weight. On the contrary, various tissues including liver, kidney, and peripheral blood cells showed variable responses of DPP-4 to SB. Especially in the kidney, although DPP-4 activity was decreased by SB in HFD-induced obese mice, it caused an increase in mRNA expression of $TNF-{\alpha}$, IL-6, and $IL-1{\beta}$. The pro-inflammatory actions of SB in the kidney of HFD-induced obese mice were recapitulated by cultured mesangial cell experiments, in which SB stimulated the secretion of several cytokines from cells. Our results showed that SB has differential actions according to its treatment dose and the type of cells and tissues. Thus, further studies are required to evaluate its therapeutic relevance in metabolic diseases including diabetes and obesity.

아동의 物活論的 思考에 관한 연구 : Piaget의 가설을 중심으로

李恩和,尹賢燮 이화여자대학교 한국문화연구원 1976 韓國文化硏究院 論叢 Vol.28 No.-

This study was intended to investigate the Korean child's animism and to test Piaget's hypothesis with Huang-Lee's experimental tools. The experimental hypotheses in this study were: 1) Piagetian animism could be found in Korean children's mentality, and 2) Piaget's hypothesis could be proved not only by Russell-Dennis's tools but also by Huang Lee's. Russell and Dennis have proved Piaget's hypothesis and standardized the experimental procedures to test child's animism; however, M. Mead and Huang-Lee have denied the Piagetian animism in Manusian and Chinese children. Russel and Dennis have also suggested four developmental stages in child's animism. Twenty Korean children, aged between 4-7, were tested individually, the combined experimental procedures of Russell-Dennis and Huang-Lee being used, As a result, the first hypothesis in this study was positively confirmed only by Russell-Dennis's method. This means that the second hypothesis in this study was not confirmed. 90% of the Subjects in this study have had the concept of life or living, and did not regard inanimate objects as having characteristics of "feeling pain." It seems to us that the subjects in this study tend to make some cognitive continuity between their awareness of their living and the being of physical objects. In Piagetian terms, the subjects have not dissociated themselves from their physical and natural environment.

인지기능과 사건관계 전위

이성훈,이종섭,한은선,유계준 大韓神經精神醫學會 1990 신경정신의학 Vol.29 No.4

Event related potential(ERP) were measured in thirty one normal controls(32.9±6.3 in average age), while two kinds of stimuli, frequent and infrequent stimuli were given with an instruction(for counting infrequent stimuli), without an instruction. The amplitudes on N1, P2, P3 were significantly higher with infrequent stimuli than with frequent stimuli in ignoring condition. In instructed condition, the significantly higher amplitudes in P1, N1, P3 components were noted with infrequent stimuli than with frequent stimuli. The amplitude of P3 increased most prominently in an attending condition comparing to an ignoring condition. Good performers of this task showed significantly higher amplitudes in N1 and P3 than bad performers.

姙娠 및 分娩에 미치는 達生散의 效果에 關한 硏究

李恩美,李京燮,宋炳基 慶熙大學校 1986 論文集 Vol.15 No.-

In order to study the effects of Dalsaengsan, reputed to be the effective for the remedy and treatment of hard labor, experiments regarding the antiabortive effect of Dalsaengsan on the pregnant rats and the effect of it on the uterine smooth muscle strips of nonpregnant rats were carried out. The results were as follows 1. The drug administered on the uterine muscle strips of nonpregnant rats caused the effect of uterine relaxation in proportion to concentration of the drug. 2. The drug administered on the uterine contraction induced by oxytocin or prostaglandin F_(2α) of nonpregnant rats caused the effect of uterine relaxation in proportion to concentration of the drug. 3. The drug administered on the antiabortive effect when the dosage of the drug was 640 ㎎/㎏. 4. The effect of extracted powder and mixture of each extracted powder was nearly the same on the relaxation effect of the drug on the uterine muscle strips of the nonpregnant rats, but the effect of the former was better than that of the latter on the antiabortive effect.

Mitochondrial DNA Polymorphism in the Korean Population

이희석,황적준,박희경,이혜린,송은섭 大韓法醫學會 1996 대한법의학회지 Vol.20 No.1

한국인 100명을 대상으로 인체게놈에서 다형성이 가장 높다고 알려진 미토콘드리아 DNA(mtDNA)의 과변이 제1 및 제2부위를 중합효소반응으로 증폭한 후 염기서열을 결정하였고, 이들로부터 97개의 mtDNA형을 확인하였다. 이들 중 세 mtDNA형은 각각2명에서 관찰되었으며, 나머지 94개의 mtDNA형은 한 개체씩에서 관찰되었다. 이 염기서열들을 Anderson등(1981)이 보고한 참고서열과 비교하여 109개의 염기부위에서 변이를 관찰하였다. 이들 중 101 염기부위 (93.2%)는 이행, 9 염기부위(6.8%)는 전환형 치환이었다. 한국인 100명에서 관찰되는 각 mtDNA형의 빈도로부터 계산된 유전자 다양성 (gene diversity)은 0.99이고, 각 염기서열의 상호비교(pairwise comparison)로 구해진 평균 염기 다양성(nucleotide diversity)은 1.05이다. 한국인에게서 관찰되는 미토콘드리아 과변이 제1과 제2부위 연기서열의 분석으로 얻을 수 있는 개체의 식별력은 0.99이므로 이는 동일한 모계관계에 있지 않은 두 개체를 식별함에 매우 유용한 것으로 생각되었다.

음식물 폐기물류로 제조된 퇴비의 고형연료 가치 평가 연구

이경섭,장한별,정은지,도아름,하진욱 순천향대학교 부설 산업기술연구소 2021 순천향 산업기술연구소논문집 Vol.27 No.2

In 2019, the daily generation of food waste in Korea was 14,314 tons, which is a steady increase compared to the past. To efficiently use food waste as an effective resource, composting and feeding are carried out. In Korea, attention is gradually focused on a sustainable resource circulation society. Compost made of food waste has problems with foreign substances, heavy metals, and salt content, and solid fuelization research is being actively conducted to compensate for this. In this study, infrared spectroscopy(FT-IR), organic content measurement(TGA), and chemical composition were measured to compare the fuel characteristics with wood pellets, which are major solid fuels, in order to examine the physical and chemical properties of food waste compost.

새로운 반복단위에 의한 GABARB1 유전좌의 염기서열다형성과 한국인에서 대립 유전자의 빈도

이혜린,이혜승,송은섭,한길로,황적준 大韓法醫學會 1998 대한법의학회지 Vol.22 No.2

To determine allele frequency of GABARB1 Jcus in Korean, first, we sequenced each allele, which was amplified with primers that published before. We found mismatching between allele number and size of each allele when 'GATA' repeats are counted. The sequence revealed new 'GATA' repeat. So we had made new allele designation with total number of in 176 Korean people. The heterozygosity this locus was 0.716 and mean exclusion chance (MEC) and power of discrimination (PD) were 0.433 and 0.845, respectively. In this study, we emphasized necessity of sequencing of 2-3 alleles, which have same size before setting up allelic ladder.

코오넬의학지수(Cornell Medical Index)에 의한 남·녀 대학생의 건강실태 조사연구

이기남,이종섭,유은주 圓光大學校 醫科學硏究所 1987 圓光醫科學 Vol.3 No.2

This study was conducted to evaluate the Health Status of 584 students enrolled in WonKwang University by Cornell Medical Index Questionaire from March to May 1987. The conclusion is as follows ; 1. Inaducacy, eyes and ears, fatigue in CMI items was showed high Complaints rate of more than 30% 2. For the purpose of understanding the Health Problem according to the general characteristics of students implemented Analysis of Variance Test. The results that according to sex, age, marital status, income, native place, medical institution looking for firstly, college are significant difference appeared. 1) Sex ; Female students shows higher complaints rate than male students. 2) Age; The younger, the higher complaints rate appeared. 3) Marital Status ; Single shows higher complaints rate than married. 4) Native Place ; Students who grown in middle, small city and large city shows higher complaints rate than rural students. 5) College ; Medical college, the others (humanistic, sociological arts college), Herb medical college, in regular sequence high complaints rate appeared.

牧丹皮 玄胡索이 姙娠에 미치는 影響

裵恩敬,李京燮,宋炳基 慶熙大學校韓醫科大學韓醫學硏究所 1995 慶熙韓醫大論文集 Vol.18 No.2

It is acknowledged that Moutan cortex radicis and Corydalis tuber are contraindicated drugs of pregnancy. The present work was done to investigate the influences of Moutan cortex radicis and Corydalis tuber on pregnancy. The extracted and condenced liquid of Moutan cortex radicis and Corydalis tuber were administered to experimental animals and the influences were evaluated on pregnant maintenance, corpus lutein functions and toxic action of kidney and liver during pregnancy. The results of this work were summerized as follows: 1. For white pregnant rats, Moutan cortex radicis showed insignificant decrease of the fertilized eggs while Corydalis tuber showed insignificant increase of the fertilized eggs as the pregnant days pass by. 2. For false pregnant rabbits, Moutan cortex radicis and Corydalis tuber showed significant decrease of HCG. 3. For white pregnant rats, Moutan cortex radicis and Corydalis tuber showed insignificant decrease of GOT, GPT and ALP levels. 4. In serum of white pregnant rats, Moutan cortex radicis showed significant decrease of BUN level while Corydalis tuber showed insignificant increase. 5. In serum of white pregnant rats, Corydalis tuber showed increase of creatinine level while Moutan cortex radicis did not show changement. 6. Moutan cortex radicis and Corydalis tuber showed insignificant increase of serum uric acid and uric protein levels of white pregnant rats.

少腹逐瘀湯의 效能에 關한 實驗的 硏究

金恩基,張峻福,李京燮,宋炳基 慶熙大學校 1995 論文集 Vol.24 No.-

This experiment was performed on analgesic, anti-pyretic, sedative, anti-swelling, anti-thrombotic, anti-tumor effect and response of isolated uterus by administrating high and low concentration of Sopokchukeotang's hot-water-extract to experimental animals. This administration purpose to investigate the efficacy of the Sopokchukeotang which is known as having effects on treating female genital diseases on basis of oriental medicine literature and its clinical use. The results obtained were as follows: 1. The writhing syndrome induced by acetic acid was decreased by administration of the Sopokchukeotang's hot-water-extract. However, there was no statistical significance between sample and control group. 2. The administration of the Sopokchukeotang's hot-water-extract elevated the pain threshold significantly by on hindpaw edema induced by 1% carrageenin in rat. 3. Abnormally elevated body temperature induced by thyphoid vaccine was decreased by the administration of the Sopokchukeotang's hot-water-extract. But, there was no statistical significance. 4. Sleeping time induced by Pentobarbital-sodium was prolonged by the administration of the Sopokchukeotang's hot-water-extract. However significant effect was recognized only in the treated by low concentration group(p<0.05). 5. The significant decrease of swelling induced by carrageenin was noted in the group treated by high concentration(p<0.05, p<0.01). 6. The group treated by low concentration showed significant anti-swelling effect on the swellings induced by yeast, but not in the group treated by high concentration didn't show it(p<0.05). 7. The serum FDP was decreased significantly by administrating the Sopokchukeotang's hot-water-extract to the experimental thrombosis(p<0.05, p<0.01). 8. The serum fibrinogen was increased significant by the administration of the Sopokchukeotang's hot-water-extract on the experimental thrombosis(p<0.01, p<0.001). 9. The prothrombin time was not influenced by the adminstration of the Sopokchukeotang's hot-water-extract on the experimental thrombosis. 10. The administration of the Sopokchukeotang's hot-water-extract did riot make a prolongation the survival in Saroma 180 ascitic tumor implanted Rats. 11. The administration of the Sopokchukeotang's hot-water-extract had no effect on the normal movement of the isolated uterus. However, it showed the relaxation effect on the oxytocin induced muscle contraction of the isolated uterus. According to the above results, it might be recognized that the Sopokchukeotang's hot-water-extract has the effect of analgesia, anti-pyrexia, sedation, anti-swelling, anti-thrombosis and relaxation effect on the oxytocin induced contraction of uterus muscle. However, anti-tumor effect of Sopokchukeotang's hot-water-extract was not appeared in the experiments. According to the above results. Sopokchukeotang's not-water-extract can be applied on the various inflammatory diseases in female genital organs.