Tryophagus putrescentiae induces inflammatory cytokine secretions from the human cell lines THP-1 and EoL-1 cells

Jeon Sang‐Jin,Kim Eun Joo,Oh Jihyun,Yun Chi‐Young 한국곤충학회 2020 Entomological Research Vol.50 No.7

The present study aimed to identify the roles of the storage mite Tyrophagus putrescentiae (Tp) in relation to human skin diseases, such as atopic dermatitis. We examined the effects of T. putrescentiae extracts (TpE) on the secretion of inflammatory cytokines and their mRNA expression in human immune cells. Human monocytic THP-1 and eosinophilic EoL-1 cells were challenged with TpE and the inflammatory cytokines, interleukin-6 (IL-6), interleukin-8 (IL-8), and monocyte chemotactic protein-1 (MCP-1) secreted in culture supernatant were analyzed using an enzyme-linked immunosorbent assay (ELISA). The mRNA levels in the cell cultures were also measured via reverse transcriptase-polymerase chain reaction (RT-PCR). In the THP-1 cells, the TpE significantly induced the secretion of IL-6, IL-8, and MCP-1 in a time- and dose-dependent manner. It also stimulated increases in the expression levels of the IL-6, IL-8, and MCP-1 mRNA. However, while the TpE induced IL-8 and MCP-1 mRNA levels in the EoL-1 cells, it did not induce IL-6 expression. Using Western blot analysis, we confirmed the activation of extracellular signal-regulated kinase (ERK), a member of the mitogen-activated protein kinases (MAPKs), in response to TpE challenge of the THP-1 cells. Collectively, our results show that TpE challenge elicits inflammatory response in the human monocytic THP-1 and eosinophilic EoL-1 cells, and it induces increased production of the inflammatory cytokines IL-6, IL-8, and MCP-1.

Conceptual Study for Tissue-Regenerative Biodegradable Magnesium Implant Integrated with Nitric Oxide-Releasing Nanofi bers

Jin‑Kyung Jeon,Hyunseon Seo,Jimin Park,Soo Ji Son,Yeong Rim Kim,Eun Shil Kim,Jong Woong Park,Woong‑Gyo Jung,Hojeong Jeon,Yu‑Chan Kim,Hyun‑Kwang Seok,Jae Ho Shin,Myoung‑Ryul Ok 대한금속·재료학회 2019 METALS AND MATERIALS International Vol.25 No.4

The excessive initial corrosion rate of Mg is a critical limitation in the clinical application of biodegradable Mg implantsbecause the device loses its fi xation strength before the fractured bone heals. This study suggests a new approach to overcomethis hurdle by accelerating tissue regeneration instead of delaying the implant biodegradation. As angiogenesis is anessential process in early bone regeneration, a Mg implant coated with electrospun nanofi bers containing nitric oxide (NO),which physiologically promotes angiogenesis, is designed. The integrated device enables adjustable amounts of NO to bestored on the NO donor-conjugated nanofi ber coating, stably delivered, and released to the fractured bone tissue near theimplanted sites. An in vitro corrosion test reveals no adverse eff ect of the released NO on the corrosion behavior of the Mgimplant. Simultaneously, the optimal concentration level of NO released from the implant signifi cantly enhances tube networkformation of human umbilical vein endothelial cells without any cytotoxicity problem. This indicates that angiogenesis canbe accelerated by combining NO-releasing nanofi bers with a Mg implant. With its proven feasibility, the proposed approachcould be a novel solution for the initial stability problem of biodegradable Mg implants, leading to successful bone fi xation.

만요(漫謠)의 선행연구와 특징

전은진(Eun-jin, Jeon) 한국문화융합학회 2021 문화와 융합 Vol.43 No.5

This paper supplements and modifies the conceptual characteristics of previous research on Manyo in “A Study on the Musical Characteristics and Reinterpreted Works of Manyo during the Japanese Colonial Period. Manyo’s previous research began when Kim Chang-nam and Cho Dong-il, and Zhang Eu-jeong started full-scale research. Through his doctoral thesis, several books, and academic papers, he focused on the appearance of the 1930s, the characteristics of lyrics, and the status of Manyo. Later, Lee Dong-soon and Park Ae-kyung focused on the appearance and lyrics of the time, while Lee Eun-jin, Lee So-young, and Jeon Eun-jin also studied the musical characteristics along with the lyrics. Since 2017, we have identified recent Manyo . For the conceptual characteristics of Manyo, the concepts were divided into the definition of Manyo, the etymology of Manyo, and the transition of Manyo and the concepts were then organized through previousresearch and basic historical data. With the missing parts and additional data from the existing study, we were able to reorganize the previous research and conceptual features of Manyo.

동적 후보영역과 이중 템플릿매칭을 이용한 차량 번호판 추출 및 인식

전진석,이은주 한밭대학교 정보통신전문대학원 2003 정보통신전문대학원 논문집 Vol.1 No.1

본 논문에서는 획득한 차량 영상에서, 차량 번호판의 후보영역을 동적으로 할당하여 번호판을 추출하고, 이중 템플릿 매칭을 이용하여 인식하는 방법을 제안하였다. 차량 번호판은, 이 영역이 다른 영역에 비해 영상의 흑백 변화 밀도가 높다는 것을 근거로, 번호판 후보영역을 투영하여 영상 밀도의 기준 밀도와의 차에 의하여 추출된다. 번호판 영역 추출을 실패하면, 다시 동적으로 가장 가능성이 높은 영역을 번호판 후보영역을 결정하였다. 추출된 번호판 영역에서 영역정보에 의하여 문자와 숫자영역을 분리하고,문자와 숫자의 인식은 분할된 입력 패턴과 표준패턴을 흑화소 정보에 의하여 1차 매칭하고, 유사도가 높은 표준패턴과 다시 백화소 정보에 의하여 2차 매칭하는 이중 템플릿 매칭에 의하여 하였다. In this paper, we proposed method to extract vehicle license plate by assigning dynamically candidate area, and to recognize using double template matching in captured vehicle image. We extracted vehicle license plate using density difference of candidate area and reference, because the image density of candidate area is high than other. If it failed to extract in this process, we determine dynamically candidate area. We recognized characters and numbers on plate by double template matching of input pattern and references. Then it matched black pixels first, and white next.

북한이탈주민의 사회적응 7년 추적 연구 : 생활과 교육을 중심으로

전우택,유시은,엄진섭,김희진 연세대학교 통일연구원 2009 통일연구 Vol.13 No.1

The purpose of this study is to analysis the change patterns of life style and education of North Korean defectors. The questionnaire of this study is for two hundred North Korean defectors who had entered South Korea in 2001. The questions are about residence, education, legal issue, and health. According to this study, the residences of the defectors were still concentrated on the Metropolitan area. Type of residence has become varied so as to increase proportion of their own-houses and long-term leases. Males had got educated for employment purpose, while females had learned using computer the most. Among age groups, the 20s and the 30s had taken regular education the most. As the length of staying in South Korea had increased, the legal cases had decreased; however, the legal issues had become more complicated. The number of the defectors with chronic diseases has gradually declined; thus, overall health status of the defectors had become better. As the length of staying in South Korea had increased, their needs for supports of basic life had decreased, while the demands for consultation about marriage and legal issues. In the conclusion, we point out several ideas based on this study result, which can be utilized in developing policies and supporting programs for North Korean defector adaptation in South Korea.

남한 내 북한이탈주민들의 3년간 사회의식 변화 및 생활 만족도에 대한 추적연구

전우택,유시은,조영아,홍창형,엄진섭,서승원 大韓神經精神醫學會 2006 신경정신의학 Vol.45 No.3

Objectives : The aim of this study was to examine the change in attitudes and satisfaction level of North Korean defectors in South Korea over a 3 year period (2001-2004). Methods : In our first study in 2001, a total of 200 detectors were interviewed. In the follow up study after 3 years, 151 defectors were re-interviewed by an in-person interview. Results : The level of satisfaction to the governmental support policy to defectors decreased significantly. However, the general level of satisfaction increased slightly (from 3.52 to 3.56 ; 5-score scale). This shows that North Korean defectors are stably adapting to South Korean society. The level of satisfaction significantly correlated with the defectors' confusion of the different value systems, the defectors' understanding of the South Korean people, satisfaction with their physical and mental health, and the Prejudice of South Korean people to defectors. In terms of the attitudes, the type of schooling, the military service and the communist party membership in North Korea were not related to the change in attitude. However, there was a significant correlation with gender and age. Women showed a greater change in attitude compared to men. Older people had difficulty in understanding the South Korean people, but they showed little psychological affiliation to North Korea. Moreover, they showed a high level of satisfaction with the economic situation in South Korea and had an optimistic viewpoint for their future than younger people. Even though many defectors attended churches, religion was not associated with the level of satisfaction or their change in attitude. Conclusion : The factors that influence the level of satisfaction and attitudes of North Korean defectors have changed over 3 years. Therefore, the government, nongovernmental organizations (NGOs) associated with defectors and the churches need to be more sensitive to these changes and make plans to support the adaptation of defectors to South Korean society.

KS H ISO 1242 표준의 평가 : 라반딘 정유의 산값 측정

전혜림,이은지,장혜진,손현화,김건,김남선,전명숙,이동선 서울여자대학교 2010 자연과학연구논문집 Vol.22 No.-

The acid value (AV) as well as the ester value of essential oil is an important index to evaluate quality of essential oil. The official standard analytical method of KS H ISO 1242 dealing with “Essential oils - Determination of acid value” was assessed experimentally using two lavandin essential oils obtained in France as an example. One French lavandin oil sample showed the results of AV= 0.9 by both indicator method and potentiometry. Another sample showed AV = 1.9 by indicator method and AV = 1.6 by potentiometry. Latter lavandin oil sample exhibited significantly higher acid value than maximum value of 1 described in the standard. This result suggests that latter lavandin oil was contaminated, because this oil was contained in an orange juice bottle. Results of this study proved that KS H ISO 1242 could be used as a suitable scientific standard for the determination of acid value of essential oils.

남한 내 북한이탈주민의 3년간 사회적응 추적 연구 : 2001년부터 2004년까지 생활과 교육을 중심으로 With prioty given to life of North Korea defectors from 2001 to 2004

유시은,전우택,조영아,홍창형,엄진섭 연세대학교 통일연구원 2005 통일연구 Vol.9 No.1

Purpose of this study : In 2001, this study that investigate actual conditions about adaptation degree of Korean social life of the North Korean defectors was carried out to 151 people that succeed in chases among 200 the North Korea defectors answer in study. Purpose of this study is as following. First, we wished to search change aspect of actual conditions about South Korea society adaptation of the North Korean defectors who take part in the first investigation for past 3 years. Second, we wished to search difference of adaptation aspect by populational and social element. Third, We wished to suggest things that can be beaconed to efficient support policy of government or private organization and successful South Korea social adaptation method which support the North Korean defectors on the basis of study finding about change of adaptation aspect of the North Korea defectors. Methods of Study : We designated 200 Panels to study and survey actual adaptation conditions of the North Korean defectors on South Korea society synthetically and for long-term. So, chasing about 151 people was possible in 2004. We are fertilized and supplement the questionnaire forming in 2001 and composed the new questionnaires to part of a life, mental state, Body and health. This study among them investigated a standard of living of the North Korean defectors. In this process, interviewer directly asked and recorded questions to reduce individual variation of people who receive interview. Study Results : Government's residence support policy about North Korean secession inhabitants was generally attained as effective and suitable for North Korean inhabitants' early South Korea settlement. Only, government need to discuss about North Korea secession inhabitants' residence support more variously for long-term. Second, education should be supported more actively and variously. Suitable point of education for their needs should make a effect of vocational training or education maximize. This is a very important part. Third, they need guidance and education that can receive more easily law support. Fourth, medical supports and health cares should be achieved continuously and systematically to them since they arrived in South Korea. To supports and studies North Korean secession inhabitants has the large significance to gain a foothold about reunification of North and south. Their South Korea society adaptation is becoming topic of conversation among South Korean. An atmosphere of unity is elated. And interest degrees about North Korean are actually rising as exchanges between South and North Korean is activated. Therefore, the adaptation study about North Korean secession inhabitants will have to be expanded in studies for South Korean, not to be limited in studies for North Korean.

무증상으로 발견된 담낭-담관 누공을 동반한 Mirizzi 증후군 1예

홍진희,전태주,서지영,서동대,오태훈,신원창,최원충,신은아,김기환 인제대학교 2009 仁濟醫學 Vol.30 No.-

Mirizzi's syndrome is a rare complication of the gallstone disease, characterized by narrowing of the common bile duct (CBD) due to chronic extrinsic compression of an impacted gallstone in the cystic duct or the neck of the gallbladder. The impacted gallstone may erode into the bile duct, causing cholecystocholedochal fistula. The patients generally have history of repeated attacks of jaundice and abdominal pain. But there is no report about asymptomatic Mirizzi syndrome with cholecystocholedochal fistula which were found incidentally. So herein we present our clinical experience with a case of asymptomatic Mirizzi syndrome with cholecystocholedochal fistula in a young female.

지역4-H조직의 내적 적합성에 관한 문화기술적 연구

김진모,김진군,전영욱,이정은,최영창,이은영 한국농업교육학회 2008 농업교육과 인적자원개발 Vol.40 No.2

이 연구의 목적은 지역4-H조직의 내적 적합성을 탐색하고 이를 기술하는데 있었다. 2006년 8월부터 10월까지 약 3개월 동안 지역4-H후원회 회장 및 임원진 3명, 지역4-H연맹 회장 1명, 학교4-H 지도교사 4명, 지역의 영농4-H회장 1명, 시·군 농업기술센터 소장 및 4-H담당자 9명을 주요 연구 참가자로 하여 심층면담을 통해 자료를 수집하였다. 수집한 자료를 분석한 결과는 다음과 같았다. 첫째, 지역4-H조직 관련자들이 인식하는 지역4-H조직의 주요성과 격차로, ① 민간4-H조직을 중심으로 한 자율적이고 독립적인 활동 부재, ② 지역4-H조직 간 다양한 종류의 협력 부재, ③ 지역사회에서 4-H활동에 대한 가치 인식 부족이 나타났다. 둘째, 지역4-H조직의 핵심 과업으로 ① 4-H의 가치 및 이념에 부합하고, 지역의 실정에 맞는 프로그램 확보, ② 지역4-H 조직 간 협력적 네트워크 확립, ③ 4-H에 대한 지역사회의 관심 및 지지 확보가 나타났다. 셋째, 지역4-H조직의 내적 부적합성으로는 ① 인원 확보의 어려움과 핵심과업에 대한 전문성 결여, ② 관료화되고 경직된 낡은 문화, ③ 조직적 자원의 축소 및 조직 간 낮은 네트워크의 수준이 도출되었다. The purpose of this study was to describe and understand internal fit in Local 4-H Organization. The main participants of this study were one leader and two members of Local 4-H Society for the support, one leader of Local 4-H Association, four School 4-H teacher, one leader of Local farm 4-H, 9 local agricultural extension workers. The data were collected through in-depth interview with them from August, 2006 to October, 2006. The Results from this study were as follow: 1. Key performance gaps of local 4-H organizations were identified: ① lack of local 4-H based activities, ② lack of various types of partnership between local 4-H organizations, ③ lack of understanding value of 4-H activities in the communities. 2. Key tasks of local 4-H organizations were identified: ① developing local 4-H programs based on 4-H's values and ideas, ② establishing the cooperative networks among local 4-H organizations, ③ ensuring the interest and support about 4-H in the communities. 3. Internal misalignments of local 4-H organizations were identified: ① difference of recruitment and lack of expertise, ② bureaucratic and rigid cultures, ③ decrease in organizational support and low level of network among organizations.