분자역학적 방법을 이용한 부산시내 대형건물 냉각탑수의 레지오넬라균의 형별 분류

김정만,정석훈,서대영,박은희,송은주,최재철,이은엽,장철훈 대한임상미샐물학회 2004 Annals of clinical microbiology Vol.7 No.1

TNF-α 유전자형과 방광암과의 관계

정필두,김은정,엄민식,서정원,윤석중,김종석,이상철,김원재 충북대학교 의학연구소 2001 忠北醫大學術誌 Vol.11 No.2

연구목적: TNF-α는 일부 종양의 종양화 과정과 관련이 있는 것으로 알려져 있다. 본 연구는 TNF-α 발현에 영향을 미치는 TNF-α 촉진자 -308 부위의 유전적 다형성이 방광암과 관련이 있는지 유무를 알고자 시행하였다. 대상 및 방법: 유전자 분석을 위하여 환자 113명 및 대조군 109명으로부터 혈액을 채취하여 genomic DNA를 분리한 후 PCR-RFLP 및 direct DNA sequencing을 통하여 TNF-α유전자의 다형성을 조사하여 방광암의 발생, 병기 및 분화도와 비교 검토하였다. 결과: TNF-α 촉진자 -308 부위의 유전형은 대조군에서는 GG형이 83.5%(90례 및 GA형이 16.5%(19례)로 관찰되었으며 AA형은 없었다. 환자군에서는 GG 형이 85.4%(97례), GA형 및 AA형은 각각 13.1%(15례)및 0.8%(1례)에서 관찰되었다. 두 군 모두에서 GG형이 가장 많이 나타났으며 다음으로 GA형을 보이고 AA형은 1례의 방광암 환자에서만 관찰되었다. -308부위의 경우도 두 군 사이에 유전자형의 차이는 없었다(p=0.259) 분화도별 분포를 보면 grade I이 20례, grade II가 49례, grade Ⅲ은 34례였고 병기별로 표재성인 경우가 90례였으며 침윤성은 14례였다. 분화도가 나빠질수록 GA형이 증가하였다(p=0.04). 그러나 병기와 TNF-α promoter -308부위의 유전자형 사이에는 유의한 상관 관계가 없었다(p=0.123). 결론: 방광암 환자의 혈액에서 GA genotype이 관찰되는 경우, 분화도가 나쁠 가능성이 매우 높기 때문에 좀 더 적극적인 치료와 세밀한 추적관찰을 함으로써 방광암으로 인한 사망과 암의 진행을 예방할 수 있을 것으로 기대한다. Purpose : Tumor necrosis factor-alpha (TNF-α) is involved in tumorigenesis of several cancers as an endogenous tumor promoter. The purpose of this study was to investigate whether genetic polymorphism of TNF-α promoter region (-308) was associated with human bladder tumor. Materials and Methods: The DNA from 113 and 109 respective blood samples of bladder tumor Patients and control group was analyzed by PCR-based restriction fragment length polymorphism (RFLP) and direct DNA sequencing methods to characterize the genetic polymorphism of -308 promoter region of the TNF-α gene in bladder tumor patients. We compared the association of bladder tumor with genetic Polymorphism of TNF-α promoter region(-308) in relation to the stage, grade, recurrence and progressio. Results : Eighty-six percents(97/113) of bladder tumor patients and 83.5% (90/109) of control group showed genotype GG at -308 region of TNF-α. Difference in genetic variations of TNF-α promoter (-308) did not exist between bladder tumor patients and control group(p=0.259). Tumor grade was significantly related to the GA genotype (p=0.04). The higher is the grade in bladder tumor, the more frequent was the GA genotype. Tumor stage, recurrence and progression were not significantly associated with genetic polymorphism of TNF-α promoter region (-308). Conclusion: The GA genotype of TNF-a promoter region (-308) had a significant impact on TNF-α production and was related to higher grade tumor compared to GG genotype. TNF-α serum levels in bladder tumor patients were significantly higher than controls. These data suggested that TNF-α might involve the tumorigenesis of the bladder rather than treatment or prevention of bladder tumor.

만성 강박장애 환자의 정서처리 특성 : 정신분열병 환자 및 정상인과의 비교

석정호,김재진,전종희,이홍식,안석균,이은,김찬형 大韓神經精神醫學會 2005 신경정신의학 Vol.44 No.3

Objectives : Obsessive compulsive disorder (OCD) tends to take chronic course as schizophrenia. Researchers reported that OC patients had idiosyncratic sensitivity to threat-related information, while schizophrenic patients were reported to have affec-tive blunting. We hypothesized that there might be a difference in emotional response between OCD and SPR in cronic phase. Methods : Eight different emotional tasks were done by 25 healthy controls, 21 OCD patients and 25 SPR patients. Visual stimuli were made by pairing two words or pictures. Four kinds of emotional stimuli (positive, negative, combined and neutral)were presented to subjects through monitor and they were asked to report their subjective feelings by pushing mouse button, In the combined condition, a pair of positive words (or pictures) and negative words (or pictures) were presented simultaneously. The responses and response time were recorded and analyzed using SPSS 9.0 package. Results : In the negative condition, OCD group didn't show any deficit compared to healthy group. But SPR group showed significantly lower appropriate response rate than healthy group, In the combined condition, OCD and healthy control group Showed 'negativity bias' which SPR group didn't show. In the positive condition, however, OCD and SPR groups did show Significantly lower appropriate response rate than healthy controls. Conclusion : Our results suggested that OCD patients may not have deficits in the processing of negative emotion even in the chronic phase but to have a specific deficit in positive emotion. This result supports the 'threat-relatedness hypothesis' on attentional bias of OCD.

급성 심근경색증 환자에서 혈전용해요법의 시간지연에 영향을 미치는 요인

정진옥,김윤철,성보영,김준경,전준용,류정곤,성인환,전은석 대한순환기학회 1997 Korean Circulation Journal Vol.27 No.8

모유 수유아와 조제 분유 영양아에서 수유 방법에 따른 아미노산 농도 비교 및 혈청 트립토판 농도와 수면 유도와의 관계

정다운,김은영,양은석,박상기,박영봉,문경래 朝鮮大學校 附設 醫學硏究所 2005 The Medical Journal of Chosun University Vol.30 No.2

Objective: Sleep behavior is modulated by serotonergic neurons within the brain, and the synthesis and release of serotonin by such neurons is thought to be influenced by the availability of tryptophan, the amino acid precursor of serotonin. Formula-feeding infants have depressed plasma tryptophan concentration relative to breast milk-feeding infants. Because tryptophan alters sleep waking patterns in adults, a study was designed to determine the difference of sleep latency relative to differences in plasma tryptophan and tryptophan : large neutral amino acid (LNAA) ratio between formula-feeding infants and breast milk-feeding infants. Method: 45 newborns who were born in Chosun University Hospital from December 2002 to December 2003 were selected. The newborns were divided to three group, 15 newborns were fed breast milk and 15 newborns were fed formula A, and last 15 newborns were fed formula B. At 6 weeks and 12 weeks, infants were sampled for measuring of serum amino acid level and tryptophan, tryptophan : LNAA ratio. And we taught infants' parents to measure sleep latency that means the time after a feeding to the first episode of active REM sleep that persist ≥ 1 min. Result: 1) At 6weeks, Serum α-amino-n-butyric acid, citrulline, tryptophan level was higher in formula A group than breastmilk group (P<0.05) and proline level was lower than breast milk group (P<0.05). In formula B group, serum n-amino-n-buryric acid, citrulline, cystine, hydroxylysine, hydroxyproline, isoleucine, leucine, lysine, methionine, phenylalanine, taurine, threonine, valine levels were higher than breastmilk group (P<0.05). And Serum isoleucine, methionine, proline, valine level were higher in formula B group than formula A group (P<0.05). 2) At 12 weeks, serum glutamic acid, methionine, ornithine levels were higher in formula group A than breast milk group (P<0.05). Serum β-alanine, aspartic acid, α-amino-n-butyric acid, ethanolamine, glutamic acid, threonine level were higher in formula B group than breast milk group (P<0.05). Serum β-alanine, ethanolamine levels were higher and arginine level was lower in formula B group than formula A (P<0.05). 3) At 6 weeks, serum tryptophan concentration and tryptophan: LNAA ratio were higher in formula A group (P<0.05). Sleep latency was 21 minutes in formula group A, 24 minutes in breast milk group, 25 minutes in formula B group but there was no statistically significance (P>0.05). 4) At 12 weeks, serum tryptophan concentration and tryptophan: LNAA ratio, time after feeding to the first episode of active REM sleep were no difference with each other group. Conclusion: There is significant difference of serum amino acids between breast-feeding infants and formula-feeding infants. And serum tryptophan and tryptophan: LNAA ratio differ between brest-feeding infants and formula-feeding infants. But there is no association between serum tryptophan, tryptophan: LNM ratio and sleep latency.

Resin infiltration을 시행한 법랑질 초기 우식 병소의 특성

김은영,권민석,김신,정태성 大韓小兒齒科學會 2012 大韓小兒齒科學會誌 Vol.39 No.1

The concept of resin infiltration which was born in an innovative philosophy to arrest the incipient caries. However, the structural changes of resin infiltrated lesions have not yet explained completely. The liquid resin might contribute not only to maximizing the penetration but to deteriorating physical stability. This study was performed to examine some physical and histological features of resin infiltrated incipient carious lesions. With the specimen of resin infiltrated lesions, microhardness by nanoindentation in depth profile, morphology of resin tags were revealed after HCl dissolution, and degree of microleakage were assessed. The percentage of microhardness of surface layer and lesion body of untreated specimen to sound enamel was 64.6% and 24.6% respectively, while that of resin-infiltrated lesions was 72.1%, showing significant difference (p<0.05). The resin tags observed under SEM had relatively homogeneous length of 433(282~501) ㎛ on the average. Among 20 specimens for microleakage assessment, 13 specimens showed no leakage while 5 and 2 showed leakage into outer and inner half of lesion respectively. It was thought the infiltrant resin penetrates deeply and homogeneously into lesion body and improves its hardness with relatively good physical stability. 본 연구는 infiltrant resin을 침투시킨 초기 우식 병소의 특성을 평가하기 위해 법랑질의 깊이에 따른 경도를 측정하고, 병 소 내로의 레진 침투양상을 분석하며, 미세누출 정도를 관찰하여 다음의 결과를 얻었다. 건전 법랑질에 대한 초기 우식 법랑질의 경도 백분율은 표층(5~40 ㎛) 64.6%, 병소본체(60~350 ㎛) 24.6%였으며, infiltrant resin을 침투시킨 병소의 경도 백분율은 72.1%로 나타났다. 5~350 ㎛의 전 깊이에서, infiltrant resin을 침투시 킨 병소는 비처치 우식 병소보다 경도가 유의하게 높았지만 정상 법랑질에 비해서는 경도가 유의하게 낮았다(p<0.05). FESEM 관찰 결과 탈회된 법랑질 내부로 비교적 균일하게 침투한 레진 테그가 관찰되었으며, 침투깊이는 433 ㎛(282-501)로 측정되었다. 열순환 처리 후 13개의 치아에서 미세누출이 관찰되지 않았고, 5개에서 병소 외측 절반, 2개에서 병소 내측 절 반까지 미세누출이 관찰되었다.

초고층 구조물의 기둥축소 현상에 관한 연구

윤석한,정은호 慶熙大學校 大學院 院友會 1997 高凰論集 Vol.21 No.-

Differential shortening of vertical members in high-rise buildings affect other structural members that have to be considered such as horizontal members and exterior cladding of many elements which affect the total amount of shortening, different loading history mainly comes from the different construction time. Shortening of 66 story concrete column and wall were investigated and compared according to the different construction time. Little difference was found between the total shortening of column and wall.

월경전증후군의 진단분류학적 개념 및 원인과 치료 : Nosology, Etiology, and Treatment

문석우,서정석,류은정,남범우 건국대학교 의과학연구소 2004 건국의과학학술지 Vol.14 No.-

Since the emergence of the term premenstrual syndrome (PMS) in the 1950s, PMS has become an increasingly discussed topic in popular media sources. Thus self management techniques are easily accessed by women through the media or through their peers. Premenstrual dysphoric disorder (PMDD) comprises extremely distressing emotional and behavioral symptoms. Women with PMDD report premenstrual symptoms, primarily mood symptoms, that are severe enough to seriously interfere with their life style and relationships and usually do not respond to conservative and conventional interventions. Although women with PMS are most often seen in primary care or by their obstetricians/gynecologists, the result of recent study suggested a high rate of missed diagnoses. The premenstrual syndromes include the more common PMS, the less prevalent PMDD, and menstrual psychosis. The etiology of PMS and PMDD is still largely unknown. That PMS and PMDD are primarily biological phenomena is underscored by recent, convincing evidence. Therapeutic interventions for PMS and PMDD range from the conservative to treatment with psychotropic medication and, for the more extreme cases, hormonal therapy or surgical procedures.

위 전절제술을 받았던 환자에서 아급성 연합성 변성 1예

한정호,하현욱,이정석,송은향,김우정,김두응,최진영 건국대학교 의과학연구소 2000 건국의과학학술지 Vol.10 No.-

Subacute combined degeneration is a metabolic disorder of the spinal cord caused by vitamin B12 deficiency. A 72-year old man was admitted with weakness and tingling sensation of the bosh upper extremities. 9 years ago, he was diagnosed as having stomach cancer. So total gastrectomy with splenectomy was done at that time. The complete blood cell counts and morphology revealed megaloblastic anemia. Nerve conduction velocity study(NCV), electoromyography(EMG) and somatosensory evoked potentials(SEP) showed diffuse sensorimotor peripheral neuropathy. Magnetic resonance imaging(MRI) showed an increased T2-weighted signal, mild decreased T1-weighted signal, and slight contrast enhancement of the posterior columns of the cord in the cervical and upper thoracic spinal region. He was treated with vitamin B12 supplements and improved gradually. If abnormal sensation and muscle weakness was developed after gastrointestinal surgery, subacute combined degeneration should be considered in the differential diagnosis. We describe MRI findings of a typical case of subacute combined degeneration, which was induced by total gastrectomy.

주요 4개국 전자정부 비교·분석 : 한국, 미국, 영국, 일본을 중심으로

류석상,이연우,박정은 한국정보사회진흥원 2003 정보화정책 Vol.10 No.2