Association of 20 potential ATP2B1‐interacting genes with blood pressure in Koreans

Kyung‐Won Hong,Hyun‐Seok Jin,Ji‐Eun Lim,Bermseok Oh 한국유전학회 2011 Genes & Genomics Vol.33 No.3

Plasma membrane calcium‐transporting ATPase 1 (ATP2B1)is associated significantly with blood pressure in Caucasians and Asians. ATP2B1 regulates calcium homeostasis and belongs to the P‐type calcium pump family; several studies have identified diverse proteins that bind to ATP2B1. We hypothesized that ATP2B1 regulates blood pressure through ATP2B1‐interacting genes. To this end, 20 potential ATP2B1‐interacting genes were selected, 197 SNPs of which were analyzed for their association of systolic and diastolic blood pressure. These 20 genes were categorized into 2 groups: ATP2B1‐binding genes and ATP2B1‐cleaving calpain family members. Three ATP2B1‐binding genes (CALM1, NOS1, and PDLIM1)were associated with blood pressure, and a SNP in CALM1(rs2401887) generated the strongest association signal (beta=‐3.60±0.92, p=8.9×10^(‐5) for systolic blood pressure and beta=‐1.40±0.62, p=0.02 for diastolic blood pressure). Of the calpain family members, 3 genes (CAPN6, CAPN9, and CAPN10)were associated with blood pressure, and the CAPN10 SNP rs4676348 yielded the strongest association signal (beta=‐0.88±0.27, p=0.001 for systolic blood pressure and beta=‐0.58±0.18, p=0.015 for diastolic blood pressure). Further, the interaction of CALM1 to ATP2B1 was examined using the blood pressure of individuals who carried both variants of CALM1and ATP2B1 genes. Similarly the interaction of CAPN10 to ATP2B1 was also examined. The CALM1 variant (rs2401887)and CAPN10 variants (rs4676348) appear to decrease blood pressure further in addition to the decrease by the variant (rs17249754) of ATP2B1, which suggests that ATP2B1 might regulate blood pressure through the ATP2B1‐interacting genes CALM1 and CAPN10.

정신분열병환자의 뇌량에 대한 신경병리적 고찰 : 확산텐서영상(Diffusion Tensor Image)기법의 적용

이경진,신용욱,하태현,이종민,김인영,김선일,문원진,정은철,박해정,오정은,박지영,강경훈,권기원,권준수 大韓神經精神醫學會 2005 신경정신의학 Vol.44 No.4

Objectives : Diffusion tensor imaging (DTI) is a new technique that can be used to visualize and measure the diffusion of water in brain tissue. It is particularly useful for evaluating white matter abnormalities. In this paper, we investigated the neuropathology of the corpus callosum in patients with schizophrenia through the new methods, diffusion tensor imaging. Methods : Diffuse tensor imaging was performed in 19 schizophrenic patients and 26 healthy controls. One complementary measure, fractional anisotropy (FA), which is considered to be sensitive indices of axonal integrity, was obtained from regions of interest in the five areas of the corpus callosum. Neuropsychological measurement also underwent for measurement of frontal lobe function in two groups. Results : There were no significant differences of FA in the DTI measures for either the schizophrenic patients compared with controls. FA was significantly decreased in women compared with men. Neuropsychological measurements such as Wisconsin card sorting test were correlated with FA of patients with schizophrenia. Conclusion : FA measures revealed no differences between schizophrenia and controls. The findings suggest that the structural integrity of white matter in patient with schizophrenia was not disrupted. The results are discussed in relation to previous studies reporting positive and negative findings. It is concluded that further studies using DTI in larger samples, improved and standardized methods of data acquisition and analysis are needed.

중추성 요붕증이 동반된 랑게르한스 세포 조직구증 1예

김진호,문준성,문선중,이지은,최재원,은미정,천경아,조인호,윤지성,원규장,이경희 신덕섭,이형우 영남대학교 의과대학 2005 Yeungnam University Journal of Medicine Vol.22 No.2

Central diabetes insipidus (DI) is a syndrome characterized by thirst, polydipsia and polyuria. Langerhans cell histiocytosis is one of the etiologies of DI. Recently we experienced a central DI associated with Langerhans cell histiocytosis. The 44 years old female patient complained right hip pain polydipsia and polyuria. We carried out water deprivation test. After vasopressin injection, urine osmotic pressure was increased form 109mOsmol/Kg to 327mOsmol/Kg (300%). Brain MRI showed a thickened pituitary stalk and at hot bone CT.CT guided biopsy revealed abnormal histiocytes proliferation and abundant lymphocytes, The final diagnosis was central DI associated with systemic Langerhans cell histiocytosis invading hip bone, L-spine and pituitary stalk. Desmopressin and etoposide chemotherapy were performed to the patient.

신경 교차연결 후 흰쥐 골격근섬유의 마이오신 동질형의 변화

이종은,이원택,강유선,박경아 대한해부학회 1994 Anatomy & Cell Biology Vol.27 No.3

Capsaicin이 동통억제효과를 나타내는 기전에 관한 연구

이종은,김봉옥,이원택,윤호,박경아 대한해부학회 1995 Anatomy & Cell Biology Vol.28 No.5

폴리아민이 흰쥐 복재신경 재생에 미치는 영향 - Ⅰ. 수초신경섬유의 직경과 수초 두께의 변화

이종은,문혜원,이원택,박경아 대한해부학회 1994 Anatomy & Cell Biology Vol.27 No.3

개의 아토피 피부염에 대한 Leflunomide의 임상적 적용 1례

홍은지,윤기영,서경원,최호정,정성목,송근호,조종기,박성준 忠南大學校 獸醫科大學 附設 動物醫科學硏究所 2012 動物醫科學硏究誌 Vol.19 No.1

A Shih-tzu(8-year-old, castrated male) was referred to the Veterinary Medical Teaching Hospital of Chungnam National University with the history of relapsing pruritus, recurrent dermatitis. On the physical examination, generalized erythema, otitis externa, alopecia and lichenification were observed. Using Favrot's criteria for diagnishing atopic dermatitis, 7 criteria were satisfied out of 8 criteria. After treatment for infection, intradermal skin test was performed(positive allergen : Df, Dp). And then immunotherapy was performed, but clinical signs were not getting better. Next treatment of glucocorticoid shown little improvement of clinical signs, and adverse effect(diarrhea) was shown after cyclosporine application. So application of leflunomide which is used for human atopic dermatitis treatment in some case was planned. After 6 months of application of leflunomide, clinical signs were decreased gradually.

비만을 동반한 위암 환자에서 두 병을 같이 수술 치료한 경험(증례)

최경현,윤기영,문형환,신연명,서경원,안수미,송윤미,석정희,정경연,이은하 고신대학교의과대학 2008 고신대학교 의과대학 학술지 Vol.23 No.3

Obesity is growing problem in Korea. We had a case of bariatric surgery during gastric cancer operation. Patient was 29 year old Korean lady with early gastric cancer located in the lesser curvature side of the middle 1/3 of the stomach. Her body weight was 89 kg, height 163 cm, and thus body mass index was 33.5 kg/m2. Preoperative blood pressure was 130/90 mmHg, hemoglobin 12.9 g/dL, total lymphocyte count 3,280/mL, serum albumin 4.3 g/dL, CEA 1.1 ng/mL, CA 19-9 9.1 ng/mL, and alpha fetoprotein 2 ng/mL. Another associated disease was right thyroid follicular neoplasm. The chest X-ray was normal. After IM injection of 2,500 units of heparin 30 minutes before the induction of general anesthesia, she received curative subtotal gastrectomy and Roux en Y gastrojejunostomy when a malabsorption loop of 120 cm jejunum was incorporated between the Treitz ligament and the end to side jejuno-jejunostmy site at May 29th 2006. Her postoperative course was uneventful except a minor wound seroma and the elevations of serum amylase(up to 4 folds) and lipase(up to 2 folds). She lost her body weight 9 kg in 5th, 21 kg in 7th, and 27 kg in 10th postoperative months to became 61.5 kg. On follow up exams in July 2008, she gained 4 kg to overcome her weakness and fatigue. From the above result, the incorporation of a malabsorption loop during reconstruction phase of gastric cancer operation was a good option for obese gastric cancer patients especially in cases of early gastric cancer.

허혈성심질환 환자에 있어 혈액 응고상태의 비교

이미경,송경은,이원길,김재식,박의현,전재은 경북대학교 병원 1997 경북대학교병원의학연구소논문집 Vol.1 No.1

연구배경: 최근에 지혈응고 및 섬유소 용해기능의 손상이 허혈성 심질환의 병인론에 중요한 역할을 한다고 알려져 왔다. 이에 저자들은 허혈성심질환군과 정상대조군에서 PT, aPTT, 섬유소원, α2-antiplasimin, tPA, PAI-I, AT Ⅲ, protein C, XL-FDP를 측정하여, 이들이 허혈성심질환의 발병에 미치는 영향 및 중요성에 연구하고, 혈전용해제 투여전과 투여후의 결과를 비교 분석하여 이들의 검사지표로서의 임상적인 유용성에 관하여 알아보고자 하였다. 방법: 39예의 정상대조군과 급성 심근경색 및 협심증으로 진단된 40예의 환자군에서 platelet-poor-plasma를 얻어 PT, aPTT, 섬유소원은 ELECTRA 1000C(Medical laboratory automation, Inc., USA)를 이용하여 측정하였고, α2-antiplasmin는 색소법으로 SPECT-ROLYSER α2-antiplasmin(Biopool AB, Canada)kit를, tPA와 PAI-1은 면역효소법으로, Protein C는 응고법으로 Bioclot?? Protein C(Biopool AB, Canada) kit를 사용하여 측정하였다. XL-FDP는 라텍스응집법으로 DIMERTESTR Ⅱ Latex Kit(AGEN Biomedical Limited, Australia)를 이용하여 측정하였다. 결과: 환자군과 정상대조군의 비교에서 에서 PT, aPTT, 섬유소원, PAI-1 및 ATⅢ는 두 군간에 통계학적으로 유의한 차의를 나타내었고 환자군에서는 62.5%에 해당하는 25예에서 증가된 소견을 보여 주었다. 환자군에서 혈전용해제 투여전과 투여후의 결과비교에서 PT와 섬유소원에서 유의한 차이가 있었으며, α2-antiplasmin, PAI-1 및 protein C는 유의하지는 않았지만 감소하는 경향을 나타내었다. 혈전용해제 투여후 1 시간결과를 대조군과 비교해 볼 때 PT와 tPA는 유의하게 증가하였고 섬유소원과 ATⅢ는 의의있게 감소하였다. 7일째 결과는 모든 지수에서 정상대조군과 유의한 차이가 없었다. 결론: PT, 섬유소원의 검사가 허혈성 심질환의 진단과 경과관찰에 도움을 줄 수 있으며, tPA, PAI-1, ATⅢ등과 같이 관찰함으로서 진단뿐만 아니라 예후와 재발위험성에 대한 예측 등 임상적으로 중요한 검사지표로서의 가능성을 나타냄을 알 수 있었다. Background: Many studies have shown that thrombus formation has important role in the pathogenesis of ischemic heart disease(IHD) and impaired fibrinolysis is associated with an increased risk of myocardial infacrtion. Forty patients with IHD and 39 normal controls were studied about blood coagulation, fibrinolytic, inhibitory proteins to evaluate their usefulness in diagnosing, predicting prognosis and monitoring after thrombolytic therapy in IHD. Method: Platelet-poor-plasma were obtained from the 3.8% trisodium citrate treated blood and prothrombin time(PT), activated partial thromboplastin time(aPTT), firbrinogen, α2-antiplasmin, tPA,PAI-1, antithrombin Ⅲ(ATⅢ), protein C. crosslinked fibrin degradation products(XL-FDP) were measured by appropriate functional or immunological assays. Results: The IHD group showed significant increases in PT,aPTT, fibrinogen, PAI-I and XL-FDP and significant reduction in AT Ⅲ concentration. No significant changes were observed between myocardial infarction and angina pectoris subgroups in the IHD. After thrombolytic therapy, PT and fibrinogen showed significant changes. In comparsion with normal controls, post 1 hour results showed significant changes in PT, fibrinogen, tPA, ATⅢ but no significant changes in post 7 days results. Conclusion: Our findings suggest that measurements of PT and fibrinogen are helpful for diagnosis in patients with IHD. And combined with tPA, PAI-1 and AT Ⅲ, they may be useful test for monitoring after thrombolytic therapy, predicting prognosis and risk of recurrence in IHD patients.

약관절 내장증의 임상 및 방사선학적 연구

韓媛晶,金恩敬 大韓口腔顎顔面 放射線學會 1992 Imaging Science in Dentistry Vol.22 No.2

Internal derangement of the temporomandibular joint can be defined an an abnormal relationships of the meniscus relative to the mandibular condyle, articuar fossa and eminence. This may cause variable mandibular dysfunctions and pain. For diagnosis, arthrography, computed tomograght and magnetic resonance inaging are used. In this study, the author reviewed 98 TMJs of 88 patients who were diagnosed as internal derangement througth inferior joint space arthrography at the department of Oral & Maxillofacial Radiology, Dental Hospita, Dankook university through 1986 to 1992. 98 TMJs consisting of 30 disc displcement with reduction, 48 disc displcement without reduction and 20 perforation were studied about clinical and radiological findings The results were as follows ; 1. internal derangement was found most frequently in the 2nd 3rd decades and the average age of perforation was higher than that of disc displcement with higher than that of disc displcement with reduction. The sexual predilection was 2 times hiher in females. 2. The most frequent chief complaints were TMJ sound in disc displcement withreduction, pain and limitation of mouth opening in dise displcement without reduction and pain in perforation. The duration of the chief complaints was longer in dise displcement with reduction with than in preforation. The duration of the chief complaints was longer in disc displcement with reduction with than in preforatuon and disc displcement without reduction. 3. Reciprocal click was the most frequently TMJ sound in disc displcement with reduction. History of joint sound in disc displcement without reduction an crepitus in perforation was the most frequent one. 4. The average maximum opening was 45.4mm in disc displcement with reduction, 31.4mm in disc displcement without reduction and 33.8mm in perforation. 5. In the centric occlusion, posterior condylar position was the most frequent in disc displcement with reduction, posterior and concentric condylar position was frequent in disc displcement without reduction, concentric and anterior condylar position in perforation. At 1 inch opening, the same position to articular eminence was most frequently found in disc displcement with reduction, posterior position in disc displcement without reduction, posterior and anterior position in perforation was frequently found. 6. Bony changes, especially sclerosis and flattening, was most frequently found in perforation.