억제대 적용지침 개발을 통한 불필요한 억제대 적용율 감소활동

곽경선,김성은,배은경,이은숙,이은혜,최윤정,이윤경 한국의료QA학회 2009 한국의료질향상학회지 Vol.15 No.2

문제: 억제대 적용에 대한구체적 적용지침 없이 의례적으로 억제대를사용함으로 인해 환자들의 신체적 또는 정신적 안전을 위협할 수 있다. 목적: 완전 진정상태 이거나 근력이 약하여 발관능력이 없는 환자에 대한 예방적 적용, 바빠서 환자를볼수 없 는경우, 간호사본인의 판단이 없이 타인의 요구에 따르는등의 불필요한 억제대 적용이 증가하는 원인을 파악하여 중환자실의 특수성에 맞는 억제대 적용및 제거 지침을 마련하고자 한다. 의료기관: 인천광역시에 소재한 의과대학 소속병원의 집중치료실 개선효과: 억제대 적용에 대한중환자실 간호사에게 미치는 영향을 보면 태도 정도 p=0.09(p<0.1), 올바른 수행 능력은 p= 0.005 (p <0.0히로통계적으로유의한것으로 나타났다. 지식 정도 p=0.172(p<0.05) 통계적으로 유의 하지는 않지만 모든항목이 개선 활동전 에 비해 2,3차 개선 활동후에 향상한 것으로 나타났다. 개선활동전에 8.1%였던 간호 기록, 0.7%였던 간호중재, 0%였던 의사처방율이 3차 개선 활동 후 3가지 모두 100% 달성되었다. 이는 억제대 적용율 갑소 및 부적절한 억제대 적용율은 유의하게 감소하였다. 교훈: 중환자실 간호사의 억제대 적용에 대한태도, 지식, 올바른 간호 수행 능력은 지속적으로 재평가 되어지고, 교육내용은좀더 나은 간호제공을 위하여 개발 되어져야 할 것이다. 억제대 적용 기준과 제거 기준을 Check List 하여 객관성의 유지를 위한 개선 활동은계속적으로 Feedback 되어야 할 것이다.

식품 기준·규격의 합리적 관리 ·운영에 관한 연구(Ⅰ) : 두부,식용유지, 전분의 분말상 원료에 관하여 Powdered marerials of Tofu, Edible oil, Starch

김희연,홍진환,박혜경,한상배,박종석,이은주,이정성,송경희,최은희,최영준,소경아,성영제,이주엽 식품의약품안전청 2000 식품의약품안전청 연보 Vol.4 No.-

본 연구는 분말상 원료(전분, 대두분)의 저장유통시 품질변화를 건전성과 안전성 측면에서 검토하여 식품의 기준 ·규격 설정f.」 합지적으로 반영하고자 하였다. 전분과 패두분의 이물을 검사하고, 2종 대두분(중국산, 미국산)을 ?0일간 IS'c와 30t에 저장하면서 성상, 수분, 산가, 과산화물가, 지방산 조성,아플라톡신 BB의 생성여부를 분석하였다. 중국산 대두분 30'』 저장군에서 50일 이후 곰팡이가 발생한 것 이외에 모든 시료에서 양호한 성상을 나타내었고, 전분과 대두닥 모든 시료에서 이물은 검출되지 않았다. 산가는 중국산과 미국산 시료간에 편차를 보여 증국산 30"C, 50일 저장시료에서 약 10배끙도 증가하였고, 과산화물가는 저장 90일후 15'E 저장군은 약 2.3배, 30'c 저장군은 3배정도 증가하였다. 대두분의 주요 지방산은 linoleic acidf18. 2), oleic acid(18 : 1), palmitic acid(16 : 0)이었으며, 3개월 저장에 따른 지방산 함량의 변화는 미미하였다. 아플라톡신 B₁은 모든 시료에서 검출되지 않았다. The objective of this study was to investigate auality changes of type raw materials(starch, soy flour) during various storage conditions. Starchflour(source of China, U.S.A) were slfred under two temperature(15'C, 30'c) forSensory evaluation, (o.reign material test, moisture content, acid value, peroxide ualue,composition, aflatorin Bi were analyzed. China soy flour(stor·ed 30'f) was contaminated by fungj on 50 days. Except for soy flour contardnated by fungi, sensory characteristics were not changed and foreign mater;als were notdetected. Acid value of China soy flour(stored at 30'C, 90 days) was about 10 times higherlevel before storage and acid value was more influenced by source(China, USA). Peroxide valuewas in proportion with the storage period slightly. The major fatty acid of soy flour waslinoleic acid(18 . 2), an(3 the change of fatty acid composition was not observed. Aflatoxlrl Blwas not detected.

오차드그래스(Dactylis glomerata L.)의 품종에 따른 종자유래의 캘러스 형성률과, 캘러스 크기 및 식물체 재분화 효율의 비교

배은경,이인애,김기용,이병현,손대영,이효신,정민섭,조진기 Plant molecular biology and biotechnology research 2002 Plant molecular biology and biotechnology research Vol.2002 No.-

Comparison results of callus formation ratio from seed explants, callus sizes, regeneration ratios from callus and regeneration efficiency [calculated by following formular; callus formation ratio(%) × regeneration ratio(%)/100]for 27 ochardgrass (Dactylis glomerata L.)varieties imported and Hapsung 2 developed in Korea are as follows; 1. Among ochardgrass varieties showing more than 50% callus formation ratios, the descending order of callus formation ratio after bedding the seed explants for 4 weeks was 93>Sparta>Pizza>Condor>Lidaglo>Glorus>Hapsung2>Frode. 2. The callus sizes after bedding for 4 weeks were in the range of φ 0.43cm~4.2cm in which there was 10 times size difference between the largest one and the smallest one but most of them were between φ2.5cm~4cm. 3. The regeneration ratio from callus among varieties were in the range of 0~36% and descending order of the upper 6 varieties was Plano>Akimidori>Justus>Lidacta>Currie>Hall mark. 4. The regeneration efficiency which is calculated by the ratios of regeneration from seed explant numbers was between 0 to 17.4% among which Justus showed the highest value in the 4-week treatment. 5. The correlation between callus formation ratios and the callus sizes, callus formation ratios and regeneration efficiency, and callus sizes and regeneration efficiency were r=0.5765, r=0.6365 and r=0.6246, respectively in 4-week callus and all the correlations were significant on the 1% level. 6. In 6-week callus, the descending order callus formation ratios from seed sxplants for the best 6 varieties was Condor>Sparta>93M>Justus>Potomac>Lidaglo>Frode. 7. The callus sizes formed were between φ1.5~5.7cm in which Sparta, the largest one of φ5.7cm was five times larger than the smallest one. The callus size of the control variety. Hapsung 2 was φ3.8cm, which belonged to larger size. 8. Regeneration ratio showed a great deviation among varieties from 6-week old calli by showing from 0% to 100% in which all the calli were regenerated in Plano while no callus was regenerated in Juno, 9. The range of regeneration effciency was between 0~28% among varieties in which the values from 6-week callus treatment were larger than those from 4-week callus treatment. Especially, the value of Potomac in 6-week was 3 times larger than that in 4-week. 10. The correlation between callus formation ratios and the callus sizes, callus formation ratios and regeneration efficiency, and callus sizes and regeneration effciency were r=0.8369, r=0.6683 and r=0.5937, respectively in 6-week callus, and all the correlations were significant on the 1% level.

아펜탈정의 생물학적 동등성 평가

배준호,최경업,지상철,박은석 성균관대학교 약학연구소 1999 成均藥硏論文集 Vol.11 No.-

The bioequivalence of two aceclofenac tablets was evaluated in 14 normal volunteers (age 21∼29yrs) following oral administration. The test product was "Apental tablet" made by Asia Pharmaceutical Co. and the reference was "Airtal tablet" made by Daewoong Pharmaceutical Co.. After one tablet containing 100 mg aceclofenac was administered, blood was taken at predetermined time intervals and the concentration of the drug in plasma was quantitated with an HPLC method. AUC, C_max and T_max were calculated and statistically analyzed for the bioequivalence of the two products. The results showed that the differences in AUC, C_max and T_max between two products were 4.23%, 2.15% and 0%, respectively. The powers for AUC, C_max and T_max were >90%,>90% and 85.8%, respectively. Confidence intervals were within ±20% for three parameters. All of these parameters met the criteria of KFDA for bioequivalence, indicating that "Apental tablet" is bioequivalent to "Airtal tablet". (Kor. J. Clin. Pharm. 1999; 9(1): 44-48)

牧丹皮 玄胡索이 姙娠에 미치는 影響

裵恩敬,李京燮,宋炳基 慶熙大學校韓醫科大學韓醫學硏究所 1995 慶熙韓醫大論文集 Vol.18 No.2

It is acknowledged that Moutan cortex radicis and Corydalis tuber are contraindicated drugs of pregnancy. The present work was done to investigate the influences of Moutan cortex radicis and Corydalis tuber on pregnancy. The extracted and condenced liquid of Moutan cortex radicis and Corydalis tuber were administered to experimental animals and the influences were evaluated on pregnant maintenance, corpus lutein functions and toxic action of kidney and liver during pregnancy. The results of this work were summerized as follows: 1. For white pregnant rats, Moutan cortex radicis showed insignificant decrease of the fertilized eggs while Corydalis tuber showed insignificant increase of the fertilized eggs as the pregnant days pass by. 2. For false pregnant rabbits, Moutan cortex radicis and Corydalis tuber showed significant decrease of HCG. 3. For white pregnant rats, Moutan cortex radicis and Corydalis tuber showed insignificant decrease of GOT, GPT and ALP levels. 4. In serum of white pregnant rats, Moutan cortex radicis showed significant decrease of BUN level while Corydalis tuber showed insignificant increase. 5. In serum of white pregnant rats, Corydalis tuber showed increase of creatinine level while Moutan cortex radicis did not show changement. 6. Moutan cortex radicis and Corydalis tuber showed insignificant increase of serum uric acid and uric protein levels of white pregnant rats.

Helicobacter pylori 항원을 이용한 면역우유 생산 및 항체특성에 관한 연구

배만종,김수정,예은주,김병기,박창호,김미경 경산대학교 생명자원개발연구소 2003 생명자원과 산업 Vol.7 No.-

본 연구는 위염, 위궤양, 위림프종 및 위암과 같은 소화기 질환의 원인균으로 알려진 Helicobacter pylori균을 항원으로 하여 젖소에 면역시킨 후 생산된 우유의 anti-H. pylorigkdcp의 생성능을 검토하고, 백신투여량과 항체 생성과의 관계, 항원 항체의 특이성, H. Pylori균 응집력, 항체의 산과 열에 대한 안정성, 그리고 백신투여가 젖소에 미치는 영향을 알아보았다. 1. 백신 투여량에 따른 혈청과 유청내의 anti-H. pylori항체의 함량은 10㎖, 20㎖, 30㎖ 백신투여 모든 군에서 대조구에 비해서 높은 양의 항체 생성을 확인하였다. 유의적인 차이는 없었으나 20㎖투여가 항체 생성이 가장 많은 것으로 조사되었다. 백신 투여량에 따른 유청내의 anti-H. pylori항체 생성량은 혈청에서 나타난 결과와 유사한 양상으로 형성되었다. 2. Anti-H. pylori항체의 SDS-PAGE에 의한 분자량측정 결과 heavy chain은 50kDa정도, light chain은 24kDa정도로 확인 되었다. 3. H. pylori항원 단백질의 분자량측정 결과 12개의 band가 형성되었다. Anti-H. pylori의 항원 특이성을 알아보기 위해 western bloting을 한 결과 혈청, 혈청정제, 유청, 유청 정제 모두 7개의 항원성 물질을 확인할 수 있었고, 주 항 원성 물질은 분자량이 97, 66, 34kDa 이었다. 4. 응집반응결과 유청속의 anti-H. pylori항체가 H. pylori균에 대해 1/10의 응집가를 나타내었다. 5. Anti-H. pylori항체의 산·알칼리에 대한 안정성 실험에서는 pH 5 ∼ pH 10 범위에서 안정한 상태로 100%의 활성을 나타내었다. 6. Anti-H. pylori항체의 열에 대한 안정성 실험에서는 60℃에서 60분간 안정한 상태를 보였고, 70℃에서도 비교적 안정한 상태였으나 60분 경과후 40%정도 활성이 감소하였다. 80℃에서는 4분간 처리했을 때 77%의 활성을 유지하였고, 100℃에서도 1분간은 비교적 안정한 상태였다. 7. 백신투요로 인하여 유량이 12% 감소하는 경향을 나타냈고, 최장 1주일 정도 지나면서 회복되었다. 8. 백신투여 후 젖소의 체온을 측정한 결과 대조구에 비해 정상적인 범위 내에서 체온이 상승하는 경향을 나타내었다. This study has been to examine bio-function of anti-H. pylori antibodies of milk produced from cows immune with antigen germ of Helicobacter pylori and search the relation between vaccine dosage volume and antibody formation, peculiarity of antigen antibody, cohesion of H. pylori germ, stability about add and heat of antibody, and impact of vaccine dosage on cows. The content of serum and Anti-H. pylori antibody within whey in accordance with vaccine dosage volume has confirmed the formation of high-quantified antibody compared to the controlled conditions in all groups vaccine dosages of 10㎖, 20㎖, and 30㎖. It has been turned out that the antibody was farmed most in 20㎖ dosage on while there was no attention difference. The molecular weight of Anti-H. pylori antibody measured by SDS-PAGE were turned out as about 50kDa in the heavy chain and about 24kDa in the light chain. 12 bands were formed as the result of measured molecular weight of antibody protein. The western blotting was performed in order to examine the antigen peculiarity of Anti-H. pylori that all 7 antigen substances including serum, serum refining, whey and whey refining could be confirmed and the main antigen substances were 97, 66, 34kDa of molecular weight. As a result of cohesive response Anti-H. pylori antibody in whey showed 1/10 cohesive rate about H. pylori germ. In stability test about acid and alkali of antibody there was 100% activated in the range of pH 5-pH 10. In stability test about heat it showed stable condition in 60℃: for 60 minutes and comparatively stable condition in 70℃, but reduced activation to 40% after 60 minutes. It maintained 77% activation in 80℃ for 4 minutes and comparatively stable in 100℃ for I minute. It was inclined to reduce to 12% of flow caused by vaccine injection, but recovered after about maximum 1 week. In measurement of body temperature of cows after vaccine injected, it was inclined to rise with the normal scope in comparison with the controlled conditions.

지렁이 분변토로부터 황화수소 분해 균주의 분리 및 특성

배무,박상진,류희욱,조경숙,이은영 이화여자대학교 환경문제연구소 1997 이화환경연구 Vol.1 No.-

탈취제로 이용되고 있는 지렁이 분변토로 부터 황화수소를 제거하는 황산화 세균 CP-5를 분리하였다. 이 균주는 운동성이 없고 Gram 음성인 단간균 이었으며, cytochrome oxidase와 catalase test에 양성 반응을 보였다. CP-5 균주는 환원형 황합물을 기질로 하는 배지에서 독립영양적인 생장이 가능하였다. 이 균주는 thiosulfate 에서 tetrathionate를 중간생성물로 하여 최종적으로 sulfate로 산화하고, elemental sulfur를 일단 thiosulfate로 산화한 후, sulfate까지 재산화하는 것으로 사료되었다. 독립영양 배지에 yeast extract 를 첨가하면 균의 생장이 촉진되었다. CP-5 균주 현탁액에 H₂S를 2vvm의 유속으로 주입하면 140ppm까지는 거의 완전하게 제거 가능하였다. 본 연구결과로부터 CP-5 균주는 분변토에서 황화수소를 제거하는 대표적인 탈취균주이며, 황화수소 제거를 위한 생물학적 탈취 공정에 활용 가능하다는 것을 알 수 있었다. A new sulfur-oxidizing bacterium which was capable of degrading hydrogen sulfide was isolated from earthworm casts using deodorant materials. The cell of isolate CP-5 was short rod, gram negative and non-motile, the activities of cytochrome oxidase and catalase were positive. The isolate could autotrophically grow in the basal mineral medium supplemented the reduced sulfur compounds such as thiosulfate and elemental sulfur. This bacterium could oxidize thiosulfate and elemental sulfur to sulfate, and the metabolic intermediates were considered as tetrathionate and thiosulfate, respectively. The removal of hydrogen sulfide by the isolate CP-5 was confirmed, and the CP-5 was regarded as one of microorganisms responsible for degradation of hydrogen sulfide in earthworm casts. The isolate CP-5 can be applied to biodeodorization systems for the improvement of removal efficiency of hydrogen sulfide.

Human Rotavirus 위장관염에 대한 임상적 고찰

배현,이홍준,서우철,양은석,문경래,박영봉 조선대학교 1994 The Medical Journal of Chosun University Vol.19 No.2

We observed clinical features of 161 patients of acute gastroenteritis who were adimitted to the Department of Pediatrics in the Chosun University Hospital from January 1. 1989 to December 31. 1993. The stool specimen on admission was tested for rotavirus Ag by ELISA(Rotazyme Ⅱ^R)method. The results are as follows : 1) HRV antigen was detected in 97 patients (58.4%) among 161 patients with gastroenteritis. 2) In monthly distribution. 77.7% of cases developed between October and December 3) The majority if patients were under 24month of age with the peak incidnce being between 6-12 month of age. 4) Mean frequency of diarrhea per day were 5.3±2.6 days in HRV antigen rhinorrhea(48.5%), cough(44.7%). Dehydration was noted 87.2% of cases and 93.9% Was isotonic dehydration. 6) The mean duration of hospitalization was 5.3±2.6 days. 7) AST and AIT elevation in HRV group were 91.5%, 42.6% respectively. AST and ALT elevation in Non-HRV group were 73.1%, 26.9% respectively. AST elevation between two group was not correlated statistically. but ALT concentration was significantly increased in HRV group above normal than Non-HRV group (p<0.05%).

Hydroxycitrate, Carnitine 및 Capsaicin을 함유한 식이 보조제(3D-Relax Diet)의 체중 감량 효과

정은영,정경희,김미자,배윤정,장은재 동덕여자대학교 2004 생활과학연구 Vol.9 No.-

The aim of this study was to evaluate the effects of 3D-Relax Diet; a proprietary formation containing hydroxycitrate(233mg/g), carnitine(150mg/g); and capsaicin (150mg/g); on body weight, % body fat, fat mass, lean body mass, Resting Metabolic Rate(RMR), body circumference, skinfold thickness, serum glucose and lipid level. Nineteen female college students participated in this 4 weeks weight control program. All subjects were randomly assigned to the Sang sik(S) group or Sang sik & 3D-Relax(3D) group. Mean energy intake of 3D group was l,523.1±300.2 ㎉(carbohydrate: 66.3%, protein: 18.7%, fat: 15.0%) and S group consumed 1,464.8±142.1 ㎉(carbohydrate: 65.1%, protein: 19.0%, fat: 15.9%) during program. The 3D group lost 3.1±1.3 kg of body weight, 2.5±l.4 kg of fat mass, 0.6±1.2 kg of lean body mass and 16.8±183.3 ㎉/d of RMR and the S group lost 1.8±1.0 kg of body weight, 1.1±1.1 kg of fat mass, 0.8±0.3 kg of lean body mass and 19.1±181.9 ㎉/d of RMR. There was no significant differences in the loss of lean body mass and RMR between two groups, however, the total body weight and the fat mass were significantly decreased in the 3D group compared to the S group(p<0.05). The 3D group experienced a significant reduced hip size, total-cholesterol and HDL-cholesterol, however, the change of these between groups were not significantly different. There were no differences in the change of body circumference, skinfold thickness, serum glucose and lipid. These results suggest that the intake of 3D-Relax Diet(hydroxycitrate, carnitine, and capsaicin) during the weight control program decreased energy intake, fat intake and total body weight especially fat mass and those effects are presumably linked to the prevention of RMR decline and the inhibitory effects on lipogenesis.

C_4型 Oxime化合物의 立體構造와 Chemical Shift에 關한 硏究

민경은,嚴在國,朴英培 경북대학교 공과대학 1980 工大硏究誌 Vol.9 No.-

Some C_4-type oximino compounds 2-butene oxime, 2, 3-butanedione mono and dioxime, a-benzil monooxime,, r-benzil dioxime, and B-benzoin oxime were synthesized and studied. Their structures were compared with another C_4-type oximino compounds investigated already. The structure of 2-butanone oxime is in syn and anti form, 2, 3-butanedione mooooxime is in syn, and 2, 3-butanedione dioxime is in syn-syn form respectively. As the results of chemical shift changes of these compounds in various solvents, following conclusion was made, i. e., 1. Among thoes conformers, a-methyl protons are more deshielded 0.7~1.3ppm than other methyl protons which were not affected vicinal functional groups. 2. While the oximino hydroxyl proton hydrogen bonded to solvent molecule, the vicinal functional groups of aliphatic oxime were deshielded ca 0.2ppm in chemical shift, and that of B-benzoin oxime was ca. 0.3~0.4ppm. 3. The anisotropy effect of adjacent functional group to the oximino hydroxyl proton was ca 0.8ppm in case of carbonyl group, and that of phenyl ring was ca. 0.7ppm.