Effect of Ionizing Radiation on Rat Tissue: Proteomic and Biochemical Analysis

Park*, Eui‐,Chul,Yoon, Jong‐,Bok,Seong*, Jin‐,Sil,Choi, Kyoung‐,Soo,Kong, Eung‐,Sik,Kim, Yun‐,Jeong,Park, Young‐,Mee,Park, Eun‐,Mi Taylor Francis 2006 Preparative biochemistry & biotechnology Vol.36 No.1

<P>Reactive oxygen species (ROS), generated by ionizing radiation, has been implicated in its effect on living tissues. We confirmed the changes in the oxidative stress markers upon irradiation. We characterized the changes in the proteome profile in rat liver after administering irradiation, and the affected proteins were identified by MALDI-TOF-MS and ESI-MS/MS. The identified proteins represent diverse sets of proteins participating in the cellular metabolism. Our results demonstrated that proteomics analysis is a useful method for characterization of a global proteome change caused by ionizing radiation to unravel the molecular mechanisms involved in the cellular responses to ionizing radiation.</P>

Antiadhesive effect and safety of oxidized regenerated cellulose after thyroidectomy

Kyoung Sik Park,Kyu Eun Lee,Do Hoon Ku,Su-Jin Kim,Won Seo Park2,Hoon Yub Kim3,,Mi Ra Kwon1,Yeo-Kyu Youn1 대한외과학회 2013 Annals of Surgical Treatment and Research(ASRT) Vol.84 No.6

Purpose: To evaluate the antiadhesive effects and safety of an oxidized regenerated cellulose (Interceed) after thyroidectomy. Methods: Seventy-six thyroidectomized patients were prospectively randomized into two groups with regard to the use of Interceed. We evaluated each group for their adhesive symptoms using four subjective and four objective items at the 2nd week, 3rd and 6th month after thyroidectomy. All patients were examined for vocal cord motility by indirect laryngoscope at each period. Results: Total adhesion scores at each postoperative follow-up period decreased with time, but were not significantly different in each group. The median score for swallowing discomfort for liquid was significantly lower in the Interceed group than in the control group 2 weeks after surgery. In addition, the severity of skin adhesion to the trachea was reduced in the Interceed group compared with the control group 6 months after surgery. During the study, there were no adverse effects or significant differences in postoperative complications between the groups. Conclusion: Interceed appeared to be safe and effective in improving neck discomfort at early postoperative periods and preventing skin adhesion to the trachea 6 months after thyroidectomy.

RAW264.7 세포에서 interferon-r 및 LPS에 의해 유도되는 NO생성에 미치는 TALT-35의 영향

박종일,박경석,김종석,박지훈,윤은진,송경섭,서강식,김훈,윤완희,박승길,임규,황병두 충남대학교 생물공학연구소 2006 생물공학연구지 Vol.12 No.-

TALP-35 purified from human term placenta is known to increase microtubule polymerization and stabilize the polymerized microtubule. To examine the effect of TALP-35 on immune system this study was performed. MTT assay was performed to investigate the effect of TALP-35 on the proliferation of RAW264.7 cells. TALP-35 dose dependently suppress the proliferation of RAW264.7 cells at high concentration (above 1 μM) in unstimulated cells, in case of 10 μM TALP-35 treated cells the suppression was 25% but in stimulated cells it was only 15%. Cosedimentation assay was carried out to investigate whether TALP-35 can bind to tubulin of RAW264.7, monocyte/macrophage lineage of mouse, and polymerize it. TALP-35 polymerize the tubulin of RAW264.7 cells and sedimented in dose-dependent manner. To investigate the effect of TALP-35 on the expression of iNOS protein western blotting was performed. The expression level of iNOS was decreased dose dependently in high concentration of TALP-35 treatment. To examine the activity of iNOS, secreted NO was determined by method based on Griess reaction. Interferon-γ and LPS-stimulated production of NO from RAW264.7 cells was decreased dose dependently above 0.1 μM concentration of TALP-35 and 50% is decreased at 10μM of it. This study shows TALP-35 can control cytokine induced-iNOS expression therefore it might control inflammatory diseases.

후두적출자의 의사소통 태도

강수균,박선희,백은아,이현혜,최경희,강은희,박은실 대구대학교 특수교육재활과학연구소 2005 특수교육재활과학연구 Vol.44 No.3

본 연구는 후두적출자의 주된 발성방법과 후두적출 경과 시기에 따른 의사소통 태도를 살펴보고자 하였다. 연구의 대상은 성인 남자 55명으로 후두암으로 인한 후두적출자들로 구성되었다. 이들에게 실시한 의사소통 태도 설문지는 Erickson의 의사소통 태도 척도-수정판(Modified Erickson Scale of Communication Attitudes: S-24)을 본 연구에 맞게 수정 보완하여 사용하였다. 본 연구의 결과는 첫째, 주된 발성방법에 따라서는 인공후두기 사용자와 식도발성자간의 의사소통 태도에 차이가 없었다. 둘째, 후두적출 경과 시기에 따라 10년 초과된 후두적출자들과 10년 이하의 후두 적출자들 간의 의사소통 태도에 차이가 없었다. 그러므로 이들의 의사소통 태도의 긍정적 태도를 증가시키기 위하여 다양한 방법을 활용하고 습득하여 이들의 의사소통 능력을 빠르게 재습득 하여 긍정적인 의사소통 능력을 가질 수 있도록 해야 한다. The purpose of this study was to determine laryngectomee's communication attitude. 55 laryngectomees, who were in the range of 40 years old to 80 years old participated in the study. Communication attitude measurements were taken by S-24(Modified Erickson Scale of Communication Attitudes). The results showed as follows: First, score of laryngectomees with artificial larynx and esophageal speakers were no significant. Second, score of laryngectomees with over 10 years after latyngectomy and 10 years after latyngectomy were no significant.

비만학생들의 운동후 회복시 초과 산소소비량 및 지속시간에 관한 연구

박철호,권민호,고봉민,박은경,우상헌 東亞大學校附設스포츠科學硏究所 1999 스포츠科學硏究論文集 Vol.17 No.-

The purpose of this study was to examine the effect of two different intensity of continuous exercise on magnitude of excess post-exercise oxygen consumption(EPOC), energy consumption following a 20min treadmill exercise. The subjects of this study were eight super-obesity in middle school students who were above 13 years old. subjects participated in two different exercise intensity to measure respiratory gas, blood lactate concentration and eardrum temperature. The results of this study were as follows: 1. The Energy consumption of the high intensity (80% VO₂ max) exercise group (264.68 35.60cal) was greater than that of the low intensity (50%) exercise group(172.01 20.39 Cal)(p<.001). 2. The EPOC of the high intensity exercise group (22.36+ 6.82Cal ) was greater than that of the low intensity exercise group(15.39+5.27 Cal)(p<.001). 3. The EPOC duration of th high intensity exercise group(28.36=3.56min) was longer than that of the low intensity exercise group(17.23+1.25min)(p<.001) 4. The blood lactate concentration, respiratory exchange ratio and temperature of the high intensity exercise group were greater than those of the low intensity exercise group(p<.05,p<.01). EPOC and duration were increase with 80%VO₂max, 80% VO₂max as intensity exercise got higher this also showed that it had something to do with lactate concentration in blood and increase of body temperature in the conclusion. We considered that there was a difference in using methods of energy, as there was a similar difference in RER. We though this following with oxygen consumption after exercise, we could clarify that high intensity exercise was better than lower intensity exercise in sametime for those who are obese especially adolescents to maintain their weights on the aspect of effect.

이 좋은 지상의 부엌에서 : 하이네의 작품에서 보이는 음식물의 상들 Nahrungsbilder in Heines Werk

박은경 한국뷔히너학회 2004 뷔히너와 현대문학 Vol.23 No.-

Ein sorgfa¨ltiger Heine-Leser weiß,dass unza¨hlige Bilder um das Esssen und Trinken in seinem Werk zu finden sind. Warum tauchen dort die scheinbar allta¨glich-banalen, unwichtigen Nahrungsbilder so ha¨ufig auf? In welchem Kontext werden sie verwendet? Welche Funktion haben sie a¨berhaupt? Der vorliegende Aufsatz geht diesen Fragen nach, wobei der Blick auf einige wichtige Aspekte beschra¨nkt ist: auf Heines literarische Auseinundersetzung mit der großen Suppenfrage", auf seine Religionskritik, auf seine Satire auf das deutsche Volk und auf seine bunt-lebendigen Erinnerungsbilder aus der Matratzengruft". An einigen Spa¨tgedichten Heines wird gezeigt, dass der Gedanke des Volkshungers, der in der Melodie des rothen Guillotinenmarsches" bereits beim fra¨hen Heine zu finden ist, nachhaltig gewirkt hat. Der Kontrast zwischen Arm und Reich, zwischen den wohlhabenden Ma¨chtigen und den besitzlosen Ohnma¨chtigen, wird durch das konkrete Gegena¨ber der Brotlosen und der Feinschmecker und Schlemmer krass dargestellt. Heines unverleugbare Na¨he zu den Kommunisten trotz seiner Besorgnisse um Kunst und Schonheit erkla¨rt sich auch daraus, dass er das Recht des Volkes zu essen ohne wenn und aber voll anerkennen muss. Heine, der stets gegen die Usurpation des Geistes" Widerstund leisten will, setzt die Nahrungsbilder auch dafa¨r ein, das Christentum als die mit dem Thron verba¨ndete Religion, die an den Volksunterdra¨ckungsmechanismen der Herrschenden teilhat, zu kritisieren. Er spielt Brot, Suppen und Bohnen auf der Erde gegen die schalen fleischlosen Speisen im Himmel aus. Das Judentum kommt ebenfalls nicht heil davon: Durch die Hervorhebung der ja¨dischen Ka¨che wird die Bedeutung der orthodoxen ja¨dischen Religion relativiert. Weder das Christentum noch das Judentum erhalten die Fa¨rsprache des Dichters, wenn sie ohne Toleranz ihre eigene vermeintliche a¨berlegenheit behaupten wollen. Die kulinarischen Beweise des Rabbis und des Franzeskaners tragen la¨cherliche, sogar makabere Za¨ge (Disputazion). Die bunten Bilder um das Essen werden auch zur Charakterisierung der Vo¨ker verwendet. Die typischen Speisen der Deutschen vor allem beweisen sich als effektvolle Mittel zur Satire auf das deutsche Volk, das politsch zura¨ckgeblieben ist. Sie relativieren auch a¨berschwa¨ngliche patriotisch-pathetische Emotionen. Beim spa¨ten Heine bekommen die Nahrungsbilder auch etwas undere Schattierungen. Der todkranke Dichter, dem die elementare Essfunktion versagt blieb, blickt auf die paradiesische Zeit der Vergangenheit zura¨ck, wo die Speisen a¨ la Schlaraffenlund auftauchten. Bis zuletzt ka¨mmert sich der Dichter nicht etwa um die Eintrittskarte zum Himmelreich: Er vermisst lediglich the lost Paradise an earth", die Leckereien in dieser holden Erdenkliche". Somit la¨sst sich feststellen, dass die Nahrungsbilder in Heines Werk ihre Bedeutung und Funktion haben, die keineswegs zu unterscha¨tzen sind. Die nebensa¨chlich aussehenden Bilder um das Essen und Trinken tragen durchaus zur Hauptsache bei: zur Beseitigung des Volkshungers, zur ,Rehabilitation des Korpers, der Sinne, der Materie', zur Aufkla¨rung des deutschen Volkes. Bei der Betrachtung seiner absichtsvoll inszenierten, mit den hohen, erhabenen Bilder des Geistes gepaarten Nahrungsbilder sticht außerdem die heinetypische Kontrastkomik" ins Auge.

백서의 치아이동 시 피질골 천공이 치주조직의 OPG, RANK, RANKL의 발현에 미치는 영향

박우경,김성식,박수병,손우성,김용덕,전은숙,박미화 대한치과교정학회 2008 대한치과교정학회지 Vol.38 No.3

치아이동 시 피질골 천공이 치조골 재형성에 미치는 영향을 알아보기 위해서 생후 15주된 자성백서를 사용하여 피질골 천공 후 치아이동을 실시한 실험군(Tooth movement with cortical punching: TMC group, n = 16)과 교정적 치아이동만 실시한 대조군(Tooth movement only group: TM group, n = 16)의 치아주위조직을 면역조직화학염색을 통하여 관찰하였다. 실험군과 대조군의 실험동물에 20 gm의 힘으로 상악 전치부 사이를 이개시키는 치아이동을 시행하였으며 실험군에서는 상악 전치부 구개부위에 피질골 천공을 실시하였다. 치아이동 후 1, 4, 7, 14일째에 실험군과 대조군의 실험동물을 희생시켰다. 면역조직화학염색법으로 OPG, RANK, RANKL의 발현을 비교한 결과, OPG의 발현은 양 군 모두에서 미처치 대조군에 비하여 감소되었으나, 실험군에서의 발현이 대조군보다 컸으며, RANK, RANKL은 피질골 천공을 시행한 경우에 더 강한 발현을 보이는 것이 관찰되었다. 따라서 피질골 천공이 치주조직의 OPG, RANK, RANKL의 발현에 영향을 미치며 치조골의 재형성을 향상시키는 것을 알 수 있었다. Objective: The purpose of this study was to investigate whether cortical punching could stimulate the expression of OPG, RANK, and RANKL during tooth movement by immunohistochemistry. Methods: 34 sprague-dawley rats (15 weeks old) were allocated into 3 groups: TMC group (experimental group; Tooth Movement with Corticotomy, n = 16), TM group (control group; Tooth Movement only group, n = 16), and non-treatment group (n = 2). 20 gm of orthodontic force was applied to rat incisors by inserting elastic bands. The duration of force application was 1, 4, 7 and 14 days. A microscrew (diameter 1.2 mm) was used for cortical punching of the palatal side of the upper incisors in the TMC group. Results: Distributions of OPG, RANK, and RANKL were evaluated by immunohistochemistry. OPG, RANK and RANKL were observed on experimental and control groups. On the compression side, the degree of the expression of OPG decreased in both groups. The expression of RANK was most prominent in the experimental group of day 4. The expression of RANKL was most intensive and extensive in the experimental group of day 7. However, the expression of OPG was decreased in the experimental and control groups compared to the non treatment group. The expression of OPG, RANK and RANKL after force application were decreased at day 14. Conclusions: These findings suggested that cortical punching might stimulate remodeling of alveolar bone during a 2 week period of tooth movement without any pathologic change.

승강대 운동이 비만고등학생의 혈청 지질 및 아포지단백 대사에 미치는 영향

박철호,고봉민,박은경,우상헌,안민호 동아대학교 스포츠과학연구소 2000 스포츠科學硏究論文集 Vol.18 No.-

The purpose of this study was to investigate the effect of 12 wks before and after step test of obsese student in D high school in Pusan city, exercise group(7) performed 12 wks step test and control group(7), before and after subjects performed the maximal exercise thest on treadmile's 12 wks step test. Through the comparision and analysis of change serum lipids and apolipoprotein metabolism, the results of this study were as follows. 1.In 12 wks before and after step test at the rest time, exercise group was found to have a increase In TC(12.7%), TG(9.76%), LDL-C(13.8%) and to have a significant increase in HDL-C(11.3%), FFA(96.8%). 2.At the rest, the control group was found to have a little increase in TG(9.2%) and HDL-C(6.7%), and to have a significant increase in TC(9.2%), LDL-C(8.9%) and FFA(7.5%). 3.In 12 wks before and after step test at the maximal exercise, the exercise group was found to have a significant increase in TC(5.6%), HDL-C(20.2%) and FFA(54.3). 4.At the maximal exercise, were found to have a significant increase in TC(9.7%) and LDL-C(9.9%) in the control group. 5.In 12 wks before and after step test at the maximal exercise, the exercise group was found to have a significant increase in Apo A-I(8.0%). 6.Statistically, At the maximal exercise, were found to have a significant increase in Apo B(7.8%).

복강경 검사로 진단한 결핵성 복막염 1례

박민경,서원석,박재옥,김창휘,고은석,임철완 순천향의학연구소 2004 Journal of Soonchunhyang Medical Science Vol.10 No.2

Tuberculous peritonitis is discovered from sixty to ninty percent of pulmonary tuberculosis patients. Clinical symptoms are nonspecific such as mild fever, weakness and malaise but show tenderness in right lower abdomen and abdominal distension. Diagnosis is confirmed by laparoscopic and pathologic findings. We report a case of tuberculous peritonitis in an 11-year-old boy who was hospitalized because of abdominal distension with pain on right lower abdomen which had developed one day before he came to hospital. Chest X-ray showed pleural effusion on both sides. We did laparoscopic examination and observed diffuse nodular infiltration. In biopsy specimen we observed osseous granuloma and acid-fast bacilli in AFB stain.

정치 웹사이트를 대상으로 한 웹 공간 분석

박한우,한지은,최경호,김효동,Kate A. Mirandilla 한국정보사회진흥원 2006 정보화정책 Vol.13 No.1

본 연구의 목적은 필리핀의 정치 시스템 및 선거 과정에 있어 인터넷의 이용, 역할, 영향 등을 살펴봄으로써 사이버 공간에서 전개되는 정치 활동의 온라인 구조를 분석하는 것이다. 구체적으로 말하면, 본 논문은 지난 2004년의 필리핀 총 선거에서 인터넷이 이용되는 다양한 방식을 검토하여 선거에서의 인터넷의 역할을 필리핀의 정치 문화와 연관하여 탐구하였다. 방법론적으로 먼저, 지난 필리핀 선거의 웹 공간을 형성하는 여러 웹사이트들 가운데 92개가 표집되었다. 표본들을 대상으로 정보성 및 참여유도성 피쳐의 분포 현황을 운영자의 유형에 따라 조사하였다. 연구 결과에 따르면, 정당과 비정부기구에서 운영하는 웹사이트들의 역할이 두드러지는 경향이 나타났다. 정당과 비정부기구는 선거와 관련된 뉴스와 온라인 포럼의 운영 등 가장 많은 유형의 피쳐(feature)를 제공하였다. 후보자 웹사이트 또한 유권자들의 참여를 촉진하는 여러 컨텐츠와 서비스를 상당히 제공하고 있었다. 이러한 결과는 필리핀의 인터넷 정치 문화에 영향을 미친 정치 및 사회적 상황과 결부되어 논의되었다.