The Function of Dimeric Structure of Soluble Intercellular Adhesion Molecule-1 (sICAM-1): Inhibition of Leukocyte Function Associated Antigen-1 (LFA-1) Binding to ICAM-3

Choi, Suck-Chei,Han, Weon-Cheol,Park, Do-Sim,Kim, Eun-Cheol,Oh, Hyun-Mee,Oh, Jung-Mi,Jun, Chang-Duk The Korean Society for Microbiology 2002 Journal of Bacteriology and Virology Vol.32 No.1

Intercellular adhesion molecule-1 (ICAM-1) is a membrane protein, exists as a dimer on the cell surface, and interacts with leukocyte function associated antigen-1 (LFA-1), a member of ${\beta}_2$-integrin family. A soluble circulating form of ICAM-1 (sICAM-1) is also detected in human serum, and has been implicated as a regulator for LFA-1-dependent cell-cell interaction in vivo. However, previous reports demonstrated that sICAM-1 shows little inhibitory effect on LFA-1 binding to ICAM-1, indicating that sICAM-1 is unlikely to antagonize LFA-1/ICAM-1-mediated cellular events in vivo. Here, we investigated the property of the dimeric sICAM-1 as an inhibitor of LFA-1 interaction with ICAM-3, since the lower avidity of LFA-1 for ICAM-3 compared with ICAM-1 or ICAM-2 had been speculated. Using recently constructed heterodimeric sICAM-1 joined at the C terminus via an ${\alpha}$-helical coiled coil (ACID-BASE) (Jun, CD. et al., 2001, Proc Natl Acad Sci 98, 6830-6835), we also tested whether the structural integrity in dimer could affect the inhibitory action of sICAM-1. Engineered sICAM-1 dimer that contained intact ectodomain (E34/E34) significantly blocked SKW3 cell (LFA-$1^+$) binding to ICAM-3, but not to ICAM-1 and ICAM-2, indicating the lower avidity of LFA-1 to ICAM-3 than that of both ICAM-1 and ICAM-2. A one binding site knock out mutant (E34/K34) showed ${\sim}2$-fold reduction in efficiency compared with E34/E34 to inhibit cell binding. Interestingly, a one binding domain deletion mutant (E34/${\Delta}D1$-D2) showed significant reduction (${\sim}5$-fold) compare with E34/K34, suggesting that structural integrity, which is precluded in E34/${\Delta}D1$-D2, is necessary for optimal binding of dimeric sICAM-1 to LFA-1, thereby inhibiting LFA-1/ICAM-3-dependent adhesion. Furthermore, BIAcore affinity measurements revealed that E34/${\Delta}D1$-D2 bound to immobilized soluble open LFA-1 I domain with an ${\sim}3$-fold reduced affinity compared with E34/K34. Overall, our results demonstrate that maintaining the structural integrity in dimer is necessary for optimal binding of sICAM-1 to LFA-1, and further suggest the therapeutic potential of dimeric sICAM-1 to antagonize LFA-1/ICAM-3-mediated cellular events in vivo.

고위험군의 원발성 전신성 유전분증 1례 보고 및 조혈모세포이식에 대한 문헌고찰

심준,박수정,엄현석,김기영,박은정,강인중,조병식,이안희,한치화 대한조혈모세포이식학회 2001 대한조혈모세포이식학회지 Vol.6 No.2

저자 등은 클론성 형질세포질환과 동반된 젊은 연령의 원발성 전신성 유전분증 환자를 진단하였기에 다발성 골수종과의 감별 진단, 치료 , 예후 및 고용량 항암화학요법과 조혈모세포이식에 관하여 문헌고찰과 함께 보고하는 바이다. Primary systemic amyloidosis (AL) is a rapidly fatal disorder related to plasma cell dyscrasia. Conventional dose of melphalan, which prolongs the duration of survival by about 10 months, does not improve the functions of impaired organs in most cases. The high dose chemotherapy followed by autologous hematopoietic stem cell rescue for AL, in spite of its high treatment-related mortality, is a new approach to achieve high response rate and better survival. We experienced a 35-year old man with AL(involving heart, liver, stomach, kidneys, peripheral nerve, and rectum) who did not respond to the standard schedule of melphalan plus prednisone and had rapidly fatal course with organ failure. Hence, we evaluate its availability by reviewing the recent reports of high dose chemotherapy in AL.

정상 관동맥을 가진 심장 점액종 환자에서 발생한 급성 심근 경색증 1예

이정민,이현승,전은정,윤호중,정욱성,오용석,김철민,김재형,최규보,홍순조,이교영,심상인 대한순환기학회 1998 Korean Circulation Journal Vol.28 No.9

실직가정학생과 취업가정학생의 자아개념 비교연구 : IMF 이후의 현상을 중심으로

전정심,주은선 德成女子大學校 2000 德成女大論文集 Vol.31 No.-

The purpose of this study is to understand the influence of parents' unexpected unemployment on their children's self-concept after the "IMF (International Monetary Fund) Crisis" in Korea. 121 junior high school students from newly unemployed households, due to the IMF crisis, were randomly selected from the Puchun area, and they were compared to randomly selected 122 students from employed households, also in the same area. Using the Jung, W.S. (1968) "Self-Concept Questionnaire" which is the Korean version of Fitt (1965)'s, students will be compared to investigate firstly, how parents' unemployment situation influences their self-concept, and secondly, what role gender plays on their self-concept. Several suggestions based on the results will be presented in order to counsel and guide students from unemployed households.

댄스스포츠 동호인의 참여수준이 생활패턴과 대인관계에 미치는 영향

배소심,황정은 한국스포츠리서치 2004 한국 스포츠 리서치 Vol.15 No.5

The purpose of this study is to clarify the relationship between the level of participation of dance sports and the relation of lifestyles pattern and personal relationship. To complete this purpose, a total of 253 people of over 20 years old of men and women, who are participating in dance sports, were abstracted by a multi-stage stratified cluster random sampling method. A survey, which was developed by Plummer (1971) and used by Si-Bum Park (1993) and Chul-Hee Lee (1995), was used for measuring lifestyles pattern. A survey, which was developed by Nezu (1990) and used by Young-Mi Kim (1992), was reconstructed to measure personal relationship. A reliability coefficient of Cronbach's δ was gauged at .89 for a total index. As a process of the study, researchers and investigators collected survey, answeredthrough self-administering method and valid samples only were individually input into a computer and used a Multiple Regression Analysis. Analyzing with using such method and process, there are results as following. Firstly, the level of participation of dance sports partially affects lifestyles pattern Thus, the more frequent participation and the lower level of participation together build up culturally-active type. Secondly, lifestyles pattern of dance sports participantspartially affects personal relationship. Thus, the higher the lifestyles pattern of traditionally-thrifty, culturally-active, socially-active and ostentatiously-fashionable, the higher the variables of cognitive and decision-making, Also, the higher the lifestyles pattern of traditionally-thriftyand socially-active, the higher the variables of personal relationship.

地黃의 품질평가

김남재,정은아,김희정,심상범,김종우 경희대학교 동서의학연구소 2001 東西醫學硏究所 論文集 Vol.2000 No.-

The dried roots of Rehmannia glutinosa(RG) have been used in the traditional medicine for treatment of diabetes, fever and dysuria, etc. In order to investigate the quality evaluation of the dried roots of RG, we conducted the physico-chemical and biological evaluation method. The amount of catalpol from commercial samples is very diverse about 0.00~3.89% by using HPLC method, because it is easily decomposed by processing of RG. So, we should try to identify the correlation with the contents of catalpol and biological activities of RG. We chose 3 samples which were a wide difference of catalpol contents between each sample(Sample-I; 3.4%, Sample-II; 2.8%, Sample-III; 0.05%). Sample-I and Sample-II were found to be more effective than Sample-III on the DPPH free radical scavenging effect and inhibitory effect on H₂O₂-catalyzed lipid peroxidation in rat liver in vitro. And, Sample-I and Sample-II exhibited more significant effects than Sample-III on accelerating actions of the small and large intestinal transport, and diuretic action in mice. So, it is suggested that the quantitative determination of catalpol should be required for the standardization of the dried roots of RG.

지황(地黃)의 품질평가

정은아,김희정,심상범,김종우,김남재,Jung, Eun-Ah,Kim, Hee-Jung,Sim, Sang-Bum,Kim, Jong-Woo,Kim, Nam-Jae 한국생약학회 2000 생약학회지 Vol.31 No.2

The dried roots of Rehmannia glutinosa (RG) have been used in the traditional medicine for treatment of diabetes, fever and dysuria, etc. In order to investigate the quality evaluation of the dried roots of RG, we conducted the physico-chemical and biological evaluation method. The amount of catalpol from commercial samples is very diverse about $0.00{\sim}3.89%$ by using HPLC method, because it is easily decomposed by processing of RG. So, we should try to identify the correlation with the contents of catalpol and biological activities of RG. We chose 3 samples which were a wide difference of catalpol contents between each sample (Sample-I; 3.4%, Sample-II; 2.8%, Sample-III; 0.05%). Sample-I and Sample-II were found to be more effective than Sample-III on the DPPH free radical scavenging effect and inhibitory effect on $H_2O_2-catalyzed$ lipid peroxidation in rat liver in vitro. And, Sample-I and Sample-II exhibited more significant effects than Sample-III on accelerating actions of the small and large intestinal transport, and diuretic action in mice. So, it is suggested that the quantitative determination of catalpol should be required for the tandardization of the dried roots of RG.

저장 기간에 따른 사과의 품질특성과 항산화 활성 변화

진소연,심기현,이은지,구혜진,김명현,한영실,박정수,김용훈,Jin, So-Yeon,Sim, Ki Hyeon,Lee, Eun Ji,Gu, Hae Jin,Kim, Myung Hyun,Han, Young Sil,Park, Jung Su,Kim, Yong-Hoon 한국식품영양학회 2014 韓國食品營養學會誌 Vol.27 No.6

$20^{\circ}C$에서 15일 동안 저장기간별 사과의 변화를 살펴본 결과는 다음과 같다. 사과의 중량과 수분은 저장기간 경과에 따라 점차적으로 감소하는 결과를 보였으며, 사과의 저장 중 가용성 고형물 함량은 저장 4일차까지 최고치로 증가하였다가 서서히 낮아지는 경향을 나타냈다. 사과의 저장 중 유기산 산도는 저장 5일차에 급격히 감소하다가 완만히 감소하였으며, pH는 저장기간에 따라 완만히 증가하는 경향을 나타내었다. 저장 중 사과 껍질의 색상을 측정한 결과, L값은 저장기간에 따라 서서히 증가하였으며, a값은 저장 3일까지 감소하였고, 저장 5일에 증가되었다가 그 이후 점진적으로 감소하였다. b값은 저장 6일에 최저치를 보인 후 서서히 증가하는 경향을 보였다. 사과의 조직감을 측정한 결과, 저장기간이 증가됨에 따라 사과의 경도는 전반적으로 감소하였다. 저장 기간에 따른 사과의 총 페놀과 총 플라보노이드 함량을 측정한 결과, 총 페놀 함량은 저장 기간이 길어질수록 감소하는 경향이 나타났으며, 저장 3일차에서 저장 4일차에 큰 폭 감소하였다. 총 플라보노이드의 변화 결과, 1일차에 비교적 크게 감소하였고, 실험 7일차에서 8일차 사이에 급격히 감소하였다. 저장 기간에 따른 사과의 DPPH 전자공여능 측정결과, 저장기간이 증가할수록 DPPH 전자공여능은 감소하였으며, Vit. C 함량의 경우 저장 기간이 길어질수록 감소하였는데, 특히 저장 4일차에 비교적 크게 감소하였다. 사과의 관능평가 수행 결과, 저장기간이 경과함에 따라 외관의 좋은 정도는 0일차에 6.75에서 15일차 2.83으로 크게 감소하였으며, 신맛의 좋은 정도는 저장 기간에 따라 유의적인 차이를 보이지 않았으나, 전반적으로 감소하는 경향이었다. 단맛의 좋은 정도와 아삭함의 좋은 정도에서는 0일차에 각 6.25, 5.33으로 가장 높았으며, 15일차에 각 3.92, 2.83으로 유의적으로 감소하였다. 종합적인 기호도에서는 7일차와 8일차 사이에 5.00에서 3.50으로 비교적 큰 폭 감소하였으며, 15일차에 2.00으로 저장 기간에 지남에 따라 유의적으로 감소하였다. 이상의 실험결과, 사과 저장 시 3~4일차에 유의적인 차이를 보였으며, 저장 8일 이후에는 모든 항목에 있어 측정감이 크게 변화하는 경향을 나타내었다. 따라서 $0{\sim}1^{\circ}C$에서 장기 저장한 사과를 $20^{\circ}C$ 전후에서 저장 및 판매할 경우, 최고의 품질을 위해서는 3일 내에 판매할 것을 권장하며, 8일 이내에 재고를 소진할 수 있도록 유통 기간을 조정하는 것이 바람직할 것으로 사료된다. This study was performed in order to compare the changes in Korean apples' qualities and antioxidant activity when stored at room temperature, $20^{\circ}C$, for fifteen days. The results showed that the weight, moisture content, soluble solid content, acidity, and hardness levels decreased as the storage period increased. However, pH and color value L increased as the storage period increased. Total phenol contents, total flavonoids contents, DPPH free radical scavenging activity, and vit. C contents of apples significantly decreased with increased storage period. Sensory parameters, such as appearance, sweetness, sourness, crunchiness, and overall acceptability of apples, also decreased as the storage period increased. Based on these results, if, the apple are being stored around $20^{\circ}C$, then, to have the highest quality, the apples should be sold within three days, We also suggested that it is desirable not to store the apples fore more than eight days.

Discordance between Physician and the General Public Perceptions of Prognostic Disclosure to Children with Serious Illness: a Korean Nationwide Study

Kim, Min Sun,Lee, Jihye,Sim, Jin-Ah,Kwon, Jung Hye,Kang, Eun Joo,Kim, Yu Jung,Lee, Junglim,Song, Eun-Kee,Kang, Jung Hun,Nam, Eun Mi,Kim, Si-Young,Yun, Hwan-Jung,Jung, Kyung Hae,Park, June Dong,Yun, Yo The Korean Academy of Medical Sciences 2018 JOURNAL OF KOREAN MEDICAL SCIENCE Vol.33 No.49

<P><B>Background</B></P><P>It is difficult to decide whether to inform the child of the incurable illness. We investigated attitudes of the general population and physicians toward prognosis disclosure to children and associated factors in Korea.</P><P><B>Methods</B></P><P>Physicians working in one of 13 university hospitals or the National Cancer Center and members of the general public responded to the questionnaire. The questionnaire consisted of the age appropriate for informing children about the prognosis and the reason why children should not be informed. This survey was conducted as part of research to identify perceptions of physicians and general public on the end-of-life care in Korea.</P><P><B>Results</B></P><P>A total of 928 physicians and 1,241 members of the general public in Korea completed the questionnaire. Whereas 92.7% of physicians said that children should be informed of their incurable illness, only 50.7% of the general population agreed. Physicians were also more likely to think that younger children should know about their poor prognosis compared with the general population. Physicians who opposed incurable illness disclosure suggested that children might not understand the situation, whereas the general public was primarily concerned that disclosure would exacerbate the disease. Physicians who were women or religious were more likely to want to inform children of their poor prognosis. In the general population, gender, education, comorbidity, and caregiver experience were related to attitude toward poor prognosis disclosure to children.</P><P><B>Conclusion</B></P><P>Our findings indicate that physicians and the general public in Korea differ in their perceptions about informing children of poor prognosis.</P>

The effect of Achyrantes Radix and regulatory treadmill exercise on SD rats with type 2 collagen induced arthritis

Eun Jung Kim,Gye Yeop Kim,Rack Seon Seong,Hang Jung Kim,Ki Bok Choi,Ki Cheol Sim 한국육종학회 2007 한국육종학회 학술발표회 발표요지 Vol.2007 No.6