The expression patterns of RANKL and OPG in murine tooth eruption

Hwang, Kyung-Mun,Kim, Eun-Jung,Kim, Hyun-Jung,,Kim, Young-Jin,Nam, Soon-Hyeun 大韓小兒齒科學會 2006 大韓小兒齒科學會誌 Vol.33 No.2

치아의 맹출은 치아기 (dental organ)와 치조골의 세포와 연관된 매우 복잡한 과정이다. 우선 치아 맹출이 일어나기 전에 파골세포가 치낭으로 집결하게 된다. 이러한 치낭의 역할은 파골세포와 조골세포의 상호작용으로 이루어 지는 골개조와 밀접한 관련이 있는데, 이는 치아 맹출과 연관된 많은 유전자들이 치낭에서 발현되기 때문이다. RANKL는 TNF ligand family로써 조골세포에 존재하며 파골세포의 형성 및 전구세포로 부터의 활성화를 유도한다. 이러한 RANKL은 OPG에 의해 그 작용이 억제되며 RANKL와 OPG의 상대적인 비율이 파골세포의 형성에 영향을 미친다. 또한 Runx2유전자의 변이는 조골세포의 분화와 활성에 차질을 가져오고 결국 RANKL/OPG pathway를 통해 파골세포 형성에 영향을 줄 수 있다. 치아의 발육 및 맹출에 미치는 RANKL 및 OPG의 영향을 알아보고 Runx2와의 연관성을 알아보기 위해 in situ hybridization 방법으로 태생 1, 3, 5, 7, 9, 11일된 쥐의 하악 및 제1대구치를 사용하여 실험을 실시한 결과 RANKL, OPG, Runx2의 mRNA가 태생 1일부터 11일까지 치낭 및 치아주위조직에 특성있게 나타났다. 이중 태생 5일에서 9일 사이에 RANKL 및 Runx2는 치아의 교합면측과 하방 치조골 부위의 발현이 강하게 나타난 반면 OPG는 약한 발현을 보였다. 이는 또한 파골세포의 활성부위를 알아보기 위해 TRAP염색을 실시하여 태생 5일에서 9일 사이에 최대의 활성화를 나타낸 결과와 연광성 있게 나타났다. RANKL, OPG, Runx2의 특성있는 발현양상들을 종합해 볼 때, 치아 맹출은 치낭, 치아기, 치조골 사이의 상호 작용을 통해 이루어 지며, 이는 치낭이 치아 맹출에 있어서 매우 중요하다는 것을 의미한다. 또한, 이러한 유전자들 (RANKL, OPG, Runx2) 이 치아의 맹출에 중요한 역할을 하는 것으로 사료된다. Tooth eruption is a complex and tightly regulated process that involves cells of the tooth organ and the surrounding alveolus. Osteoclast precursors must be recruited into the dental follicle prior to the onset of eruption. This function of dental follicle may be regarded as the ability of bone remodeling characterized by the interaction of osteoclasts and osteoblasts. This is because tooth eruption is a localized event in which many of the genes required for eruption are expressed in the dental follicle. RANKL is a membrane-bound protein that is a member of the TNF ligand family, which is present on bone marrow stromal cells and osteoblasts, and induces osteoclast formation and activation from precursor cell. The biologic effect of RANKL is inhibited by OPG and, in bone, the relative ratio of RANKL and OPG modulates osteoclastogenesis. To evaluate the roles of RANKL and OPG in tooth eruption and the relations with the expression pattern of Runx2, in situ hybridization was performed with mandibles of mice at postnatal stage 1, 3, 5, 7, 9 and 11. mRNA of RANKL, OPG, and Runx2 are expressed in dental follicle and surrounding tissue from P1 to 11. To determine the sites of osteoclastic activity duing tooth eruption, mandibles were dissected. Peak osteoclastic activity in alveolar bone along the occlusal and basal regions was observed from P5 to 9, with osteoclasts in these regions being large and strongly TRAP-positive. The specific spatic-temporal expression patterns of RANKL, OPG and Runx2 in our study suggest that tooth eruption could be progressed through the interactions of molecular signaling among dental follicle, dental organ and alveolar bone, furthermore it means that dental follicle is quite important in tooth eruption. In addition, it indicates that these genes (RANKL, OPG, and Runx2) play critical roles in tooth eruption.

DIS80의 遺傳子型 決定을 위한 PCR法의 條件에 관한 硏究

鄭宰安,黃迪駿,李羲碩,宋垠燮 大韓法醫學會 1994 대한법의학회지 Vol.18 No.1

The polymerase chain reaction(PCR) is known to be a very sensitive method of nucleic acid synthesis by which a particular segment of deoxyribonucleic acid(DNA) can be specifically amplified. However, as no single protocol will be appropriate to all situations, it requires to optimize the PCR conditions for a given application, especially analytical procedure to amplified fragment length polymorphism of VNTR region. Recently, typing of VNTR regions in the field of forensic science is almost done by PCR, but this reaction ofter results in a number of potential problems including generation of recombinant alleles during the extension phase. The objective of this paper is to evaluate the cause of PCR products of a abnormal length, and to set up the guideline for the reliable production of D1S80VNTR region in the human genome by studying several parameters that influence polymerase chain reaction. In the study of D1S80 VNTR region to be amplified, the following PCR conditions are optimal in the 50㎕ of reaction volume and under the temperature conditions of 95℃ for 1 minute, 65℃ for 2 minutes and 72℃ for 2 minutes. 1. The adequate concentration of MgCl₂ is within the range of 1.0 to 2.0 mM. 2. The high activities of Taq polymerase show about 0.5 to 1.5 units without generation of the undesired products. 3. The adequate amount of template DNA is about 30 to 50ng, although the template DNA can be amplified with the minimum of 40pg. 4. With the 50ng of template DNA, the adequate numbers of PCR cycle range 25 to 27 cycles. 5. Artifact production by PCR is minimal within the range of 0.2 to 0.3μM of each primer concentration. With the above conditions, amplified fragment length polymorphism (AMP FLP) obtained by polymerase chain reaction from the genomic DNAs of 4 Korean families (one family with 8 offsprings and both parents, 3 families composed of each one child and both parents) were analyzed to test the applicability of this technique to paternity testing. The results obtained present reliable parent child relationships based on the correct genotypes of D1S80, without any undesired PCR products of abnormal length that are postulated as heteroduplex resulting from cross annealing or hybridization between the amplified target DNA.

급성 골수성 백혈병 환자에서 관해 유도 항암 화학요법 후 발생한 베르니케 뇌증

황진원,정재현,서광원,최병진,송영진,김종윤,이은지,임홍규,이상민 인제대학교 2009 仁濟醫學 Vol.30 No.-

Wernicke's encephalopathy is characterized by the triad of ocular symptoms, ataxia, and mental confusion and neuropsychiatric condition generally caused by acute thiamine deficiency. Although it is common in the severe alcoholics, several other causes also have been identified, such as total parenteral nutrition (TPN) use, hyperemesis gravidarum, anorexia nervosa, hemodialysis, uremia, and acquired immunodeficiency syndrome(AIDS). Chemotherapy in patient with malignanacy and long term total parenteral nutrition following bone marrow transplantation are related with Wernicke's encephalopathy. A 48-year-old woman had been treated with Daunorubicin and Cytarabine for remission induction chemotherapy of acute myeloblastic leukemia and intravenous hyperalimentation due to persistent hematochezia and ileus caused by infiltration of leukemic cell in terminal ileum. She suddenly complained of diplopia, tremor, and mental confusion at 32th day after chemotherapy and generalized tonic-clonic seizure at 34th day after chemotherapy. The diagnosis of Wernicke's encephalopathy was made with clinical presentations and brain MRI. Brain MR T2-weighted & Flair image revealed a high signal intensity lesions bilaterally at the medial thalamus and peri-acueductal grey matter of the midbrain. The patient was successfully treated with vitamin B1. We presented a case of acute Wernicke's encephalopathy developed after remission induction chemotherapy followed by intravenous hyperalirnentation in a patient with acute myeloblastic leukemia.

풋마늘의 冬季養液裁培에 關한 硏究

鄭恩周,吳仲烈,黃海鎭,韓相政 대구효성가톨릭대학교 응용과학연구소 1995 응용과학연구논문집 Vol.4 No.-

겨울철 新鮮한 풋마늘의 生産體系 確立으로 端境期를 解消함과 아울러 아파트 人口가 增加하고 있는 오늘날 室內에서 簡單한 方法으로 풋마늘을 生産하는데 必要한 資料를 얻고자 하였다. 1) 萌芽率은 15±1℃區와 20±1℃區의 Hyponex와 水道물에서 瀋陽種(暖地型)이 瀋種 7日後에, 그리고 濟州種(暖地型)이 播種 21日後에 各各 100%를 나타내었다. 그러나 義城種(寒地型)은 15±1℃區에서 播種 7日後에 全혀 萌芽하지 않았고 21日後에 15±1℃區와 20±1℃區에서 各各 85% 및 95%의 萌芽率을 보였다. 2) 草長은 15±1℃區보다 20±1℃區에서, 그리고 水道물보다 Hyponex에서 더 크며 그의 伸長速度가 빨랐다. 瀋陽種은 15±1℃區와 20±1℃區의 Hyponex와 水道물에서 播種 35日後에 잎이 黃化하면서 草長의 伸長이 停止되었으나 濟州種과 義城種은 伸長을 繼績하였다. 瀋陽種은 다른 2品種에 比하여 草長이 顯著히 컸으며 伸長速度가 매우 빨랐다. 3) 播種 21日後의 葉數, 葉?徑, 葉重 및 葉?重은 15±1℃區보다 20±1℃區에서, 그리고 水道물보다 Hyponex에서 더 增加하였다. 供試 3品鍾中 瀋陽種이 各處理區에서 葉數가 顯著히 많고 葉重이 무서웠다. 義城種은 15±1℃區의 Hyponex와 水道물에서 葉伸長을 볼 수 없었다. 4) 比生長率(CGR)은 15±1℃區와 20±1℃區의 Hyponex와 水道물에서 瀋陽種과 濟州種은 播種7日後에, 그리고 義城種은 播種 14日後에 極大期를 나타낸 다음 漸次 減少하였다. 極大期의 比生長率은 15±1℃區보다 20±1℃區에서, 그리고 水도물보다 Hyponex에서 더 높았다. 5) 20±1℃區에서 CGR의 잎으로서 寄與率은 瀋陽種과 濟州種이 播種21日後까지 增加한 다음 漸次 減少하는 傾向을 보였으나 義城種은 繼績 增加하였고, 뿌리로의 寄與率은 3品種 모두 播種14日後부터 急激히 減少하였다. 6) 葉面積指數는 20±1℃區에서 3品種 모두 水道물보다 Hyponex에서 높았다. 播種28日後의 葉面積指數는 濟州種이 2.0 그리고 義城種이 1.0이었는데 比하여 瀋陽種은 4.0 以上이었다. 7) 本實驗의 어느 境遇에 있어서도 瀋陽種이 濟州種 및 義城種에 比하여 萌芽率가 빠르고 草長이 크고 葉收量이 많았으며, 15±1℃區보다 20±1℃區에서 그리고 水道물보다 Hyponex에서 生育이 良好하였다. 그리고 瀋陽種은 20±1℃區의 Hyponex와 水道물에서 播種後 14日이 經過하면 收穫이 可能하였다. The purpose of this study is to establish a production system for providing fresh leafy garlic in winter season and to obtain data for an easy method of indoor nutriculture of this plant, especially in apartment life. 1. Emergence rates in 15℃ and in 20℃ cultured by Hyponex and by tap water were 100% after 7 and 21 days from planting in Shenyang cultivar, a Southern ecotype, and in Cheju cultivar, a Southern ecotype respectively. Euisung cultivar, a northen ecotype, did not planting uner 15℃ until 7 days from planting, but emerged 85% and 95% after 21 days from planting under 15℃ and 20℃ conditions, respectively. 2. Plant lengths were higher in 20℃ and in Hyponex culture than in 15℃ and in tap water culture, respectively. Shenyang cultivar in 15℃ and 20℃ cultured by Hyponex and tap water ceased to eloongate the leaf and showed chlorosis after 35 days from planting, but Cheju and Euisung cultivars continued to elongate the leaf. The plant length of Shenyang cultivar was especially long as compared with other two cultivars and grew fast. 3. After 21 days from planting, leaf number, diameter of leaf sheath, leaf weight and weight of leaf sheath were higher in 20℃ and in Hyponex culture than in 15℃ and in tap water culture. respecively. Among three cultivars, Shenyang had much more leaves and heavy ones. 4. Comparative crop growth rate(CGR) showed at maximum after 7 days from planting in 15℃ and in 20℃ cultured by Hyponex and by tap water in Shenyang and Cheju cul-tivar while after 14 days from plamting in Euisung cultivar, then decreased gradually afterward. The CGR at maximum was higher in 20℃ and in Hyponex culture than in 15℃ and in tap water cul-ture, respectively. 5. The percentage of contribution of CGR to leaf were increased until 21 days from planting, then decreased gradually afterward in Shenyang and Cheju cultivars, but Euisung showed continu-ous increase. The percentage of contribution of CGR to root declined markedly after 14 days from planting in all three cultivars. 6. Leaf area index(LAI) were high in all three cultivars in 20℃ in Hyponex culture as compared with tap water culture. After 28 days from planting, LAI was 2.0 and 1.0 in Cheju and in Euisung cultivar respectively, comparing with above 4.0 in Shenyang cultivar. 7. In every conditions, Shenyang cultivar exceeded in emerging date, plant length and leaf produc-tion, and the growth was better in 20℃ and in Hyponex culture than in 15℃ and in tap water cul-ture, respectively. After 14 days from planting, Shenyang cultivar was possible to harvest in 20℃ cultured by Hyponex and by tap water.

정신지체아를 위한 한국무용 적용가능성에 관한 연구

황명자,함정은 한국스포츠리서치 2004 한국 스포츠 리서치 Vol.15 No.5

The aim of this study is to know the applicability of Korean Dance Program for children with mental retardation. It was used to refer various research and examination in order to accomplish the purpose of this study. The results were summarized as follows; First, the Korean Dance Program is a possibility program for children with mentalretardation when trying to consider the quality and an effect of the Korean Dance Program. Second, The SO-GO Dance and Mask Dance of Korean Dance Program was the best popular program by boys, SO-GO and Gang-Gang Sool Lae was the best popular program by girls.

선천성 완전방실차단이 동반된 신생아 루프스 1예

정재현,이은지,이은주,황진원,송영진,최병진,김일환,김종윤,허민영,김동욱 인제대학교 2009 仁濟醫學 Vol.30 No.-

Neonatal lupus is a rare syndrome occurring In neonates with tranplacentally acquired maternal autoantibodies, In particular anti-SSA/Ro-SSB/Ra antibodies. Manifestations include congenital heart block, cutaneous lesions, thrombocytopenia, neutropenia, pulmonary or neurological disease and hepatitis. The non-cardiac manifestations resolve by 6 months of age, congenital heart block is irreversible. We experienced a case of neonatal lupus with congenital complete heart block in a newborn of asyptomatic mother with anti-SSA/Ro-SSB/Ra antibodies.

예비 초등교사와 예비 특수초등교사의 과학교수에 대한 태도 연구

황순영,정은영,이미아 국립특수교육원 2006 특수교육연구 Vol.13 No.2

본 연구는 예비 특수초등교사와 예비 초등교사의 과학교수에 대한 태도를 살펴보기 위하여 부산, 경남에 있는 교육대학과 특수교육과예 재학중인 3, 4학년 학생들을 대상으로 하였다. 예비교사의 과학교수에 대한태도를 측정하기 위해 Thompson과 Shrigley()985)가 개발한 과학교수에 대한 태도척도(Revised Science Attitude Scale)를 예비 특수초등교사와 예비 초등교사에 맞게 수정하여 사용하였다. 연구문제에 따라 기술통계와 t-검증, 이원변량분석을 실시하였다. 연구결과를 정리해 보면 첫째, 예비 특수초등교사와 예비 초등교사의 과학교수에 대한 전체 태도에서는 통계적으로 유의미한 차이가 없는 것으로 나타났다. 그러나 예비 특수초등교사가 예비 초등교사들보다 과학교육의 필요성을 보다 높게 지각하고 있는 것으로 나타났다. 둘째, 과학관련 과목 수강 여부에 따른 예비교사의 전체 태도는 유의미한 차이가 없는 것으로 나타났으나, 하위영역 중. 과학교수에 대한 편안함 영역에서는 과학관련 교과목을 수강한 경험이 있는 예비 교사가 더 긍정적인 태도를 보였으며, 필요성 영역에서는 오히려 수강한 경험이 없는 예비 교사가 더 긍정적인 태도를 나타냈다 셋째, 예비 초등교사와 예비 특수초등교사의 과학교수 태도에 대한 문항별 차이를 살펴본 결과, 예비 초등교사는 예비 특수초등교사에 비해 학생들의 질문에 대한 두려움이 크며, 예비 특수초등교사는 다른 교과와 비교해 과학의 중요성에 대해 더 긍정적인 태도를 가지고 있었다. The purpose of this study was to investigate attitude toward science teaching between elementary preservice teacher and special elementary preservice teacher in 3 and 4 grade students of university. For this purpose, the Revised Science Attitude Scale developed by Thompson & Shrigley(1986) was used and the method, t-test, two way ANOVA were used to investig science teach ing attitude for them. The results are as followed : First of all, the reliability if measurement instrument was verified through a pre-study. For the first research question was used. And Discriminative Analysis was used to investigate the second research question. The results of this study were as follows: First, according to the result of t-test, there weren't significant difference of science teaching attitude between two groups. But special elementary preservice teachers higher perceived necessity of science education than general elementary preservice teachers. Second, there weren't also significant difference of science teaching attitude between two groups according to experiences of taking science related course. However elementary preservice teachers who are taking science course feel so easy toward comfort to science teaching compared to the not taking science course.

주부들의 의복 관여도와 유아복 잡지광고에 대한 태도 고찰

황춘섭,오은정 慶熙大學校 1996 論文集 Vol.25 No.-

This research is interested in the concept of consumer's fashion involvement which makes influences on the consumer's information-searching pattern. The main object of the approach is the mothers of the babies under the age 3, who can be considered the actual babies' costumes buyers. The writer tried to find out whether the mother's own fashion involvement is in any proportionate relation with their babies' costumes involvement and also to study their various attitudes toward the magazine advertising of babies' costumes. The writer hope this research could provide babies' costumes industries with basic data that can help to establish more effectual advertising strategies. To achieve the purpose of the present research. The writer established 4 more detailed study points as follows: 1) to find out the appeal patterns of babies' costumes advertisements especially favored by the mothers 2) to find out the relationship between the mothers' own fashion involvement and their involvement in the babies'costumes advertisement 3) to find out their various degree of involvement in the babies' costumes advertisement according to their fashion involvement degree. This research was conducted through the survey method using stimulating objects and questionnaires. The experimental advertisements used in this survey were selected among the actual magazine advertising, and reproduced with the help of an expert in advertisement production who is a present member of an advertising company. During the reproduction process, all the brand names were deleted in order to control the brand familiarity and the brand preference which might have an influence on the consumer's attitude toward specific advertisements. The appeal patterns of the magazine advertising materials used in this research was limited to only 3 kinds that is, the appeal to reason, the mixed appeal to reason and emotion, the appeal to emotion. The questionnaire includes question items designed to identify the attitudes toward advertisements, the degree of fashion involvement, the opinions on utility of magazine advertising of babies costumes, and the present magazine subscription situation, the source of information on babies' costumes purchase, 394 samples were extracted through the convenience sampling method among the mothers of babies under the age 3. presently living in Seoul. The data was analyzed by Frequency, paired t-test, Chi-square, Correlation and Oneway. The result of the research can be summarized as follows: 1) Among the 3 kinds of advertisement appeal patterns, most preference was shown to the mixed appeal to reason and emotion. The appeal to emotion followed next and then comes the appeal to reason. 2) The higher the degree of mothers' own fashion involvement, the higher their involvement in their babies'costumes. 3) Mothers' fashion involvement degree doesn't make any difference in their attitudes toward the advertisement of babies' costumes.

축구선수의 Detraining과 Retraining이 혈청지질 및 호르몬농도에 미치는 영향

정정화,박재현,채종훈,성혜련,황지인,윤미숙,노금선,윤종관,윤영학,노순덕,정경숙,박일규,김은희,박현태,박상갑 대한스포츠의학회 1999 대한스포츠의학회지 Vol.17 No.1

The purpose of this study was to investigate the effects of detraining and retraining on serum lipid and hormones in soccer players. Subjects were seven male high-school soccer players. V˙O_2max was determined for each subjects by administering a treadmill test(initial speed: 90m/min, grade: 5%, increasing speed per 3 min: 30m/min). Serum lipid(T-C, TG, HDL-C LDL-C) and hormones(epinephrine, norepinephrine, growth hormones, cortisol) were assayed pre and post detraining in 10, 20, 30 days after retraining. The repeated ANOVA was used to determine significant differences. The 0.05 level of significance was as critical level for the study. The results of the study were as follows: 1. V˙O_2max(ml/min) were 3576.3±204.2ml/min pre detraining, 3234.1±198.9 ml/min post detraining. There are significant(p<.05) difference between pre and post detraining. In 10, 20, 30 days after retraining, V˙O_2max(ml/min) were 3601.4±170.9 ml/min. There were significantly(p<.05) increased in retraining periods. 2. V˙O_2max(ml/kg/min) were significantly(p<.05) decreased from 62.3±2.9 ml/kg/min to 55.9±4.7 ml/kg/min in detraining. In 10, 20, 30 days after retraining, V˙O_2max(ml/kg/min) were 62.4±3.4ml/kg/min, 62.7±2.3ml.kg/min, 67.3±7.2ml/kg/min respectively. There were significantly(p<.05) increased in retraining periods. 3. T-C were significantly (p<.05) increased from 166.6±8.5mg/dl to 175.3±10.3 mg/dl in detraining. In 10, 20, 30 days after retraining, T-C were 160.1± 3.2mg/dl, 156.7±3.7mg/dl, 140.3±9.0mg/dl. There were significantly(p<.05) decreased in retraining periods. 4. HDL-C were 61.4±6.6mg/di pre detraining, 5.3±6.6mg/dl post detraining. There are significant(p<.05) difference between pre and post detraining. In 10, 20, 30 days after retraining, HDL-C were 56.9±7.1mg/dl, 56.4±9.2mg/dl, 57.7±9.1mg/dl respectively. There were no significant difference in retraining periods. 5. The hormones(epinephrine. norepinephrine, growth hormone, cortisol) were changed as same patterns. Epinephrine were 26.0±7.0[g/ml pre detraining, 24.6±3.2pg/ml post detraining. In 10, 20, 30 days after retraining, epinephrine were 26.9±5.6pg/ml, 30.6±6.2pg/ml, 29.4±5.6pg/ml respectively. There were no significant difference in retraining periods. In conclusion, HDL-C, epinephrine, norepinephrine, growth hormone and cortisol were decreased, T-C, LDL-C and TG were increased in detraining. But HDL-C, epinephrine, norepinephrine, growth hormone and cortisol were increased, T-C, LDL-C and TG were decreased in retraining.

고립성 폐 결절로 나타난 경화성 혈관종의 폐엽 절제술에 의한 치험 1예 : A case report

황은구,이송암,황재준,김상윤,권혁중,김요한 건국대학교 의과학연구소 2004 건국의과학학술지 Vol.13 No.-

Patient was sixty-eight years old female. She was transferred to our department to resect the solitary pulmonary nodule on right lower lobe. Intraoperative frozen-section revealed bronchioloalveolar carcinoma. So, we performed right lower lobectomy and mediastinal lymph node dissection. Permanent pathologic diagnosis of this patient was sclerosing hemangioma. We report experience of treatment of sclerosing hemagioma presenting solitary pulmonary nodule by lobectomy with the review of literatures.