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The expression patterns of RANKL and OPG in murine tooth eruption
Hwang, Kyung-Mun,Kim, Eun-Jung,Kim, Hyun-Jung,,Kim, Young-Jin,Nam, Soon-Hyeun 大韓小兒齒科學會 2006 大韓小兒齒科學會誌 Vol.33 No.2
치아의 맹출은 치아기 (dental organ)와 치조골의 세포와 연관된 매우 복잡한 과정이다. 우선 치아 맹출이 일어나기 전에 파골세포가 치낭으로 집결하게 된다. 이러한 치낭의 역할은 파골세포와 조골세포의 상호작용으로 이루어 지는 골개조와 밀접한 관련이 있는데, 이는 치아 맹출과 연관된 많은 유전자들이 치낭에서 발현되기 때문이다. RANKL는 TNF ligand family로써 조골세포에 존재하며 파골세포의 형성 및 전구세포로 부터의 활성화를 유도한다. 이러한 RANKL은 OPG에 의해 그 작용이 억제되며 RANKL와 OPG의 상대적인 비율이 파골세포의 형성에 영향을 미친다. 또한 Runx2유전자의 변이는 조골세포의 분화와 활성에 차질을 가져오고 결국 RANKL/OPG pathway를 통해 파골세포 형성에 영향을 줄 수 있다. 치아의 발육 및 맹출에 미치는 RANKL 및 OPG의 영향을 알아보고 Runx2와의 연관성을 알아보기 위해 in situ hybridization 방법으로 태생 1, 3, 5, 7, 9, 11일된 쥐의 하악 및 제1대구치를 사용하여 실험을 실시한 결과 RANKL, OPG, Runx2의 mRNA가 태생 1일부터 11일까지 치낭 및 치아주위조직에 특성있게 나타났다. 이중 태생 5일에서 9일 사이에 RANKL 및 Runx2는 치아의 교합면측과 하방 치조골 부위의 발현이 강하게 나타난 반면 OPG는 약한 발현을 보였다. 이는 또한 파골세포의 활성부위를 알아보기 위해 TRAP염색을 실시하여 태생 5일에서 9일 사이에 최대의 활성화를 나타낸 결과와 연광성 있게 나타났다. RANKL, OPG, Runx2의 특성있는 발현양상들을 종합해 볼 때, 치아 맹출은 치낭, 치아기, 치조골 사이의 상호 작용을 통해 이루어 지며, 이는 치낭이 치아 맹출에 있어서 매우 중요하다는 것을 의미한다. 또한, 이러한 유전자들 (RANKL, OPG, Runx2) 이 치아의 맹출에 중요한 역할을 하는 것으로 사료된다. Tooth eruption is a complex and tightly regulated process that involves cells of the tooth organ and the surrounding alveolus. Osteoclast precursors must be recruited into the dental follicle prior to the onset of eruption. This function of dental follicle may be regarded as the ability of bone remodeling characterized by the interaction of osteoclasts and osteoblasts. This is because tooth eruption is a localized event in which many of the genes required for eruption are expressed in the dental follicle. RANKL is a membrane-bound protein that is a member of the TNF ligand family, which is present on bone marrow stromal cells and osteoblasts, and induces osteoclast formation and activation from precursor cell. The biologic effect of RANKL is inhibited by OPG and, in bone, the relative ratio of RANKL and OPG modulates osteoclastogenesis. To evaluate the roles of RANKL and OPG in tooth eruption and the relations with the expression pattern of Runx2, in situ hybridization was performed with mandibles of mice at postnatal stage 1, 3, 5, 7, 9 and 11. mRNA of RANKL, OPG, and Runx2 are expressed in dental follicle and surrounding tissue from P1 to 11. To determine the sites of osteoclastic activity duing tooth eruption, mandibles were dissected. Peak osteoclastic activity in alveolar bone along the occlusal and basal regions was observed from P5 to 9, with osteoclasts in these regions being large and strongly TRAP-positive. The specific spatic-temporal expression patterns of RANKL, OPG and Runx2 in our study suggest that tooth eruption could be progressed through the interactions of molecular signaling among dental follicle, dental organ and alveolar bone, furthermore it means that dental follicle is quite important in tooth eruption. In addition, it indicates that these genes (RANKL, OPG, and Runx2) play critical roles in tooth eruption.
肢발생중 세포 증식성에 미치는 Etretinate의 영향에 관한 연구
황정연,김은영,박선화,전용혁 고려대학교 의과대학 1994 고려대 의대 잡지 Vol.31 No.1
Cell proliferation in the developmental sequence of fore-limb in the DDY mouse with the adverse effect of single dose of etretinate (Tigason®) on it correlated to the drug-induced dysmorphogenesis was studied by measns of argyrophil technique for nucleolar organizer regions(AgNORs) and expression of proliferating cell nuclear antigen(PCNA). The number of AgNORs per nucleus displayed regional differences in the limb bud on day 11 of gestation, as higher values in apical ectodermal ridge(AER) and growing tip but decreased rapidly thereafter compared with those in the other parts of epithelia and subepithelial mesenchyme, while in core mesenchyme where the higest in its value on day 11, the numbers decreased depend not on the age but on the chondrified stage specific. Antibody reaction to PCNA expressed in almost all of the cells, stronger in mesenchyme than in epithelia on day 11 and then decreased gradually with the development proceed. Siver reactions to NORs as well as PCNA expression were decreased sharply by etretinate but recovered gradually from 24 hours after treatment except the value of AgNORs in the chondrified mesenchyme. The results Indicate that growth conducted by AER with subridge mesenchyme and chondrification in the core mesenchyme are two of major processes in the early development of limb, and the adverse effects of etretinate on proliferation and differentiation persist longer in the chondrified area but decreased expression of proliferative activities by the drug do not appear to be the result subsequent to the drug-induced cell death. The results suggest that temporal inhibition or interruption of the proliferation could deprive the programmed sequence of development of its appropriate timing with subsequent disturbance of the pace in growth which might be a role of limb dysmorphogensis but the drug's affinity to the chondrified cells remains a question.
이중혈류유발 심폐소생술이 심정지를 유발한 개의 단기 생존율에 미치는 영향
황성오,조준휘,강구현,김성환,문중범,이강현,이승환,윤정한,최경훈,홍은석 대한응급의학회 2000 대한응급의학회지 Vol.11 No.3
Background and Objectives: We previously reported that, compared with standard cardiopulmonary resuscitation(S-CPR), better hemodynamic effects could be achieved by simultaneous sterno-thoracic cardiopulmonary resuscitation(SST-CPR) in which we compressed the sternum and constricted the thorax circumferentially during the systolic period by using a device. This study was designed to assess whether SST-CPR, compared with S-CPR, improve the survival rate of dogs with cardiac arrest. Subjects and methods: Twenty-five mongrel dogs(19∼31㎏) were enrolled in this study. After four minutes of ventricular fibrillation induced by an AC current, animals were randomized to resuscitate with either S-CPR(n=13) or SST-CPR(n=12). Epinephrine(1 ㎎) was injected into the right atrium every three minutes after the beginning of CPR. Defibrillation was attempted after 6 minutes of CPR. Standard advanced cardiac life support was started if defibrillation was not successful. Results: SST-CPR resulted in significantly(p<0.001) higher systolic arterial pressure(91±47 vs 47±24 ㎜ Hg), diastolic pressure(43±24 vs 17±10 ㎜ Hg), coronary perfusion pressure(35±25 vs 13±9㎜ Hg), and end tidal CO2 tension(9±4 vs 3±2 ㎜ Hg). Two of 13 animals(15 %) resuscitated with S-CPR and six of 12 animals(50%) resuscitated with SST-CPR survived until 12 hours after cardiac arrest(p<0.05). Donclusion: SST-CPR, compared with S-CPR, improves the short-term survival rate in canine cardiac arrest.
예비 초등교사와 예비 특수초등교사의 과학교수에 대한 태도 연구
황순영,정은영,이미아 국립특수교육원 2006 특수교육연구 Vol.13 No.2
본 연구는 예비 특수초등교사와 예비 초등교사의 과학교수에 대한 태도를 살펴보기 위하여 부산, 경남에 있는 교육대학과 특수교육과예 재학중인 3, 4학년 학생들을 대상으로 하였다. 예비교사의 과학교수에 대한태도를 측정하기 위해 Thompson과 Shrigley()985)가 개발한 과학교수에 대한 태도척도(Revised Science Attitude Scale)를 예비 특수초등교사와 예비 초등교사에 맞게 수정하여 사용하였다. 연구문제에 따라 기술통계와 t-검증, 이원변량분석을 실시하였다. 연구결과를 정리해 보면 첫째, 예비 특수초등교사와 예비 초등교사의 과학교수에 대한 전체 태도에서는 통계적으로 유의미한 차이가 없는 것으로 나타났다. 그러나 예비 특수초등교사가 예비 초등교사들보다 과학교육의 필요성을 보다 높게 지각하고 있는 것으로 나타났다. 둘째, 과학관련 과목 수강 여부에 따른 예비교사의 전체 태도는 유의미한 차이가 없는 것으로 나타났으나, 하위영역 중. 과학교수에 대한 편안함 영역에서는 과학관련 교과목을 수강한 경험이 있는 예비 교사가 더 긍정적인 태도를 보였으며, 필요성 영역에서는 오히려 수강한 경험이 없는 예비 교사가 더 긍정적인 태도를 나타냈다 셋째, 예비 초등교사와 예비 특수초등교사의 과학교수 태도에 대한 문항별 차이를 살펴본 결과, 예비 초등교사는 예비 특수초등교사에 비해 학생들의 질문에 대한 두려움이 크며, 예비 특수초등교사는 다른 교과와 비교해 과학의 중요성에 대해 더 긍정적인 태도를 가지고 있었다. The purpose of this study was to investigate attitude toward science teaching between elementary preservice teacher and special elementary preservice teacher in 3 and 4 grade students of university. For this purpose, the Revised Science Attitude Scale developed by Thompson & Shrigley(1986) was used and the method, t-test, two way ANOVA were used to investig science teach ing attitude for them. The results are as followed : First of all, the reliability if measurement instrument was verified through a pre-study. For the first research question was used. And Discriminative Analysis was used to investigate the second research question. The results of this study were as follows: First, according to the result of t-test, there weren't significant difference of science teaching attitude between two groups. But special elementary preservice teachers higher perceived necessity of science education than general elementary preservice teachers. Second, there weren't also significant difference of science teaching attitude between two groups according to experiences of taking science related course. However elementary preservice teachers who are taking science course feel so easy toward comfort to science teaching compared to the not taking science course.
쌍발 복합재 비행기의 속도, 고도 보정 및 상승성능에 관한 연구
황명신,박윤진,이정모,김칠영,은희봉 한국항공운항학회 1997 한국항공운항학회지 Vol.5 No.1
Airspeed and altimeter calibration of "win Mee was conducted by the flight test. We have adopted system to system method. Flight test data is corrected for instrumented error and position error, and the resultin data was satisfied. Climb Performance flight test also was conducted. But we could not have all data because of limited flight time. The resulted data was satisfied compare with calculated dab.
뇌동맥류 파열에 의한 급성 경막하 혈종이나 뇌내혈종을 동반한 혼수상태 환자에 대한 뇌혈관 조영술을 시행하지 않은 응급수술
황수현,박용규,백선하,박인성,김은상,정진명,한종우 대한신경외과학회 1996 Journal of Korean neurosurgical society Vol.25 No.10
Neurologically based clinical grading scales offer excellent prognostic information for the patient suuffering diffuse subarachnoid hemorrhaging. These grading scales are less applicable to patients with life-threatening intraparenchymal or subdural hematomas after aneurysmal rupture. During 2 and half years. 9 patients(ICH;5.SDH:1. ICH+SDH:3) in a comatose state with brain stem compression syndromes documented by computerized tomographic scans have undergone emergent operation without angiography in our neurosurgical departement. Each patient was so critically ill that we believed it unwise to delay craniotomy for diagnostic angiography. The average delay from the detection to operation was 2 hours. The origin of the hematoma was identified as a berry aneurysm of the middle cerebral artery bifurcation in seven patients and a posterior communicating artery aneurysm in two patients. One patient had a good recovery. two patients recovered with hemipardsis and mild cognitive dysfunction. two patients were vegetative and died due to pneumonia. and four patients died due to brain swelling within two weeks postoperatively. Emergent craniotomy with empiric exploration of appropriate subarachnoid cisterns after hematoma decompression may be life-saving management in some cases. The delay imposed for diagnostic angiography may be avoided in attempts to save vital minutes of severe brain stem compression.
주부들의 의복 관여도와 유아복 잡지광고에 대한 태도 고찰
황춘섭,오은정 慶熙大學校 1996 論文集 Vol.25 No.-
This research is interested in the concept of consumer's fashion involvement which makes influences on the consumer's information-searching pattern. The main object of the approach is the mothers of the babies under the age 3, who can be considered the actual babies' costumes buyers. The writer tried to find out whether the mother's own fashion involvement is in any proportionate relation with their babies' costumes involvement and also to study their various attitudes toward the magazine advertising of babies' costumes. The writer hope this research could provide babies' costumes industries with basic data that can help to establish more effectual advertising strategies. To achieve the purpose of the present research. The writer established 4 more detailed study points as follows: 1) to find out the appeal patterns of babies' costumes advertisements especially favored by the mothers 2) to find out the relationship between the mothers' own fashion involvement and their involvement in the babies'costumes advertisement 3) to find out their various degree of involvement in the babies' costumes advertisement according to their fashion involvement degree. This research was conducted through the survey method using stimulating objects and questionnaires. The experimental advertisements used in this survey were selected among the actual magazine advertising, and reproduced with the help of an expert in advertisement production who is a present member of an advertising company. During the reproduction process, all the brand names were deleted in order to control the brand familiarity and the brand preference which might have an influence on the consumer's attitude toward specific advertisements. The appeal patterns of the magazine advertising materials used in this research was limited to only 3 kinds that is, the appeal to reason, the mixed appeal to reason and emotion, the appeal to emotion. The questionnaire includes question items designed to identify the attitudes toward advertisements, the degree of fashion involvement, the opinions on utility of magazine advertising of babies costumes, and the present magazine subscription situation, the source of information on babies' costumes purchase, 394 samples were extracted through the convenience sampling method among the mothers of babies under the age 3. presently living in Seoul. The data was analyzed by Frequency, paired t-test, Chi-square, Correlation and Oneway. The result of the research can be summarized as follows: 1) Among the 3 kinds of advertisement appeal patterns, most preference was shown to the mixed appeal to reason and emotion. The appeal to emotion followed next and then comes the appeal to reason. 2) The higher the degree of mothers' own fashion involvement, the higher their involvement in their babies'costumes. 3) Mothers' fashion involvement degree doesn't make any difference in their attitudes toward the advertisement of babies' costumes.
고립성 폐 결절로 나타난 경화성 혈관종의 폐엽 절제술에 의한 치험 1예 : A case report
황은구,이송암,황재준,김상윤,권혁중,김요한 건국대학교 의과학연구소 2004 건국의과학학술지 Vol.13 No.-
Patient was sixty-eight years old female. She was transferred to our department to resect the solitary pulmonary nodule on right lower lobe. Intraoperative frozen-section revealed bronchioloalveolar carcinoma. So, we performed right lower lobectomy and mediastinal lymph node dissection. Permanent pathologic diagnosis of this patient was sclerosing hemangioma. We report experience of treatment of sclerosing hemagioma presenting solitary pulmonary nodule by lobectomy with the review of literatures.
급성 골수성 백혈병 환자에서 관해 유도 항암 화학요법 후 발생한 베르니케 뇌증
황진원,정재현,서광원,최병진,송영진,김종윤,이은지,임홍규,이상민 인제대학교 2009 仁濟醫學 Vol.30 No.-
Wernicke's encephalopathy is characterized by the triad of ocular symptoms, ataxia, and mental confusion and neuropsychiatric condition generally caused by acute thiamine deficiency. Although it is common in the severe alcoholics, several other causes also have been identified, such as total parenteral nutrition (TPN) use, hyperemesis gravidarum, anorexia nervosa, hemodialysis, uremia, and acquired immunodeficiency syndrome(AIDS). Chemotherapy in patient with malignanacy and long term total parenteral nutrition following bone marrow transplantation are related with Wernicke's encephalopathy. A 48-year-old woman had been treated with Daunorubicin and Cytarabine for remission induction chemotherapy of acute myeloblastic leukemia and intravenous hyperalimentation due to persistent hematochezia and ileus caused by infiltration of leukemic cell in terminal ileum. She suddenly complained of diplopia, tremor, and mental confusion at 32th day after chemotherapy and generalized tonic-clonic seizure at 34th day after chemotherapy. The diagnosis of Wernicke's encephalopathy was made with clinical presentations and brain MRI. Brain MR T2-weighted & Flair image revealed a high signal intensity lesions bilaterally at the medial thalamus and peri-acueductal grey matter of the midbrain. The patient was successfully treated with vitamin B1. We presented a case of acute Wernicke's encephalopathy developed after remission induction chemotherapy followed by intravenous hyperalirnentation in a patient with acute myeloblastic leukemia.
정재현,이은지,이은주,황진원,송영진,최병진,김일환,김종윤,허민영,김동욱 인제대학교 2009 仁濟醫學 Vol.30 No.-
Neonatal lupus is a rare syndrome occurring In neonates with tranplacentally acquired maternal autoantibodies, In particular anti-SSA/Ro-SSB/Ra antibodies. Manifestations include congenital heart block, cutaneous lesions, thrombocytopenia, neutropenia, pulmonary or neurological disease and hepatitis. The non-cardiac manifestations resolve by 6 months of age, congenital heart block is irreversible. We experienced a case of neonatal lupus with congenital complete heart block in a newborn of asyptomatic mother with anti-SSA/Ro-SSB/Ra antibodies.