폴록사머-폴리아크릴산 IPNs의 약물 조절 방출

변은정,박주애,이승진,김길수 梨花女子大學校 藥學硏究所 1998 藥學硏究論文集 Vol.- No.7

Poloxamer-poly(acrylic acid)(PAA) interpenetrating polymer networks (IPNs) were prepared via matrix polymerization of acrylic acid with poloxamer prepolymer. The equilibrium swelling of poloxamer/PAA IPNs was determined in various pH medium. The swelling of poloxamer/PAA IPNs was more affected by pH difference compared with the swelling of homo PAA gel due to protonation and deprotonation of the PAA network. followed by reversible formation and dissociation of the interpolymer complex due to hydrogen bonding between acidic hydrogens and ether oxygens. Nonionic/anionic/cationic drugs were incorporated into IPN matrices as a model drug and their release behavior was studied. Nonionic drug revealed release patterns depending solely on pH dependent swelling kinetics. In contrast, the release of ionic drugs was significantly affected by ionic drug-polymer interaction as well as the swelling kinetics.

가교 폴록사머 하이드로겔 물성 및 약물 조절 방출

변은정,이승진,김길수 梨花女子大學校 藥學硏究所 1997 藥學硏究論文集 Vol.- No.6

Poloxamer, block copolymers of ethylene oxide and propylene oxide was crosslinked by diisocyanates and triisocyanates to form water-swellabel, physically strong, rubber-like elastic, high biocompatible polyurethanes. The isocyanate-hydroxyl stoichiometry was kept 1:1, but the crosslinking density was varied. The variations examined were the ratio of diisocynate and triisocyanate. The delivery of two drugs of different water solubilities from hydrogel matrices was studied. It appeared that the drug nature greatly influenced its release kinetics possibly due to drug-polymer interactions. The release profiles, however, could be modified to a great extent by adjusting the polymer network structure. Generally, the high crosslinking density was required for prolonged drug delivery.

人蔘이 卵巢摘出로 誘發된 흰쥐의 骨多孔症 예방에 미치는 효능

정국영,서부일,이은숙,변부형,신순식,박지하 대한본초학회 2004 大韓本草學會誌 Vol.19 No.1

Objectives : The present study has been undertaken to investigate the effects of Ginseng Radix on prevention of osteoporosis in ovariectomized rats. Methods : In this experiment, the rats were ovariectomized. Rats were administered by 2 kinds of medicine, Estradiol, Ginseng Radix. The levels of bone mineral density, osteocalcin, ALP, phosphorus, calcium in serum, deoxypyridinoline in urine, calcium, phosphorus, ash weight of bone, body weight and uterus weight were measured. Results : The levels of spinal bone mineral density was significantly increased in comparison with OVX group at 8 weeks in Ginseng Radix group. The levels of serum didn t show significant change in comparison with OVX group in Ginseng Radix group. The levels of urine deoxypyridinoline showed significant decrease in comparison with OVX group in Ginseng Radix group. The levels of femoral and fibula-tibial ash weight didn t show significant increase in comparison with OVX group in Ginseng Radix group. The levels of femoral and fibula-tibial calcium showed significant increase in comparison with OVX group in Ginseng Radix group. The levels of femoral and fibula-tibial phosphorus showed significant increase in comparison with OVX group in Ginseng Radix group. The levels of body weight didn t show significant changes in comparison with OVX group in Ginseng Radix group. The levels of uterus weight didn t show significant changes in comparison with OVX group in Ginseng Radix group. Conclusions : Reviewing these experimetal results, it appears that Ginseng Radix have efficacy on prevention of osteoporosis. And further study should be conducted to illustrate in depth the curing and prevention of osteoprosis.

감마선 조사와 저장 기간에 따른 건멸치류의 품질 및 열발광 특성

노정은,변명우,권중호 한국식품저장유통학회(구 한국농산물저장유통학회) 2002 한국식품저장유통학회지 Vol.9 No.1

건멸치의 위생적 품질개선을 위한 감마선 조사가 품질에 미치는 영향을 조사하였으며, 아울러 감마선 조사 멸치에 대한 검지법으로서 열발광 특성을 분석하였다. 건멸치류의 미생물 오염도는 총세균이 10^4∼10^6 CFU/g 수준으로 건멸치의 종류에 따라 큰 차이를 보였으며, 대장균군은 모든 시료에서 음성으로 나타났다. 미생물학적 품질개선을 위한 3kGy 수준의 감마선 조사는 유통조건(식품포장용 PVC film, 0.06 ㎜, 500 g)으로 포장하여 6개월 저장(15±1℃) 후에도 검출한계이하(20 CFU/g)의 수준을 유지할 수 있었다. 저장 중 건멸치의 기계적색도(L, a, b, ΔE ), 갈색도, TBA가, VBN, TMA-N, 관능적 특성 등 건멸치의 품질에 관련된 여러 인자들은 3 kGy의 감마선 조사에 의해 거의 영향을 받지 않았다. 그러나 저장기간에 따른 영향은 매우 크게 나타났으며, 이는 저장온도와 포장조건의 중요성을 뒷받침해 주었다. 건멸치의 방사선 조사 여부 확인을 위한 열발광 특성을 분석해 본 결과, 비조사구는 280∼300℃ 부근에서 매우 낮은 peak를 나타내었고, 1 kGy 이상의 감마선 조사구는 200℃ 전후에서 조사선량에 의존적(R^2=0.9933)인 높은 glow curve를 나타내어 감마선 조사 건멸치의 판별이 가능하였고, - 20℃에서 6개월간 저장 후에도 검지가 가능하였다. The effect of gamma irradiation was investigated on the microbiological and physicochemical qualities of boiled-dried anchovies (large- and smallsized) packed in PVC film (0.06 ㎜) during storage at 15±1℃ for 6 months. On the other hand, thermoluminescence characteristics of minerals extracted from the sample were analyzed to evaluated its possibility in detecting the irradiated anchovies. The samples were contaminated by microbial levels of 104∼106 CFU/g in total aerobic bacterial counts and negative in coliforms, which were different depending on the sample sizes. Gamma irradiation at 3 kGy was effective for improving the hygienic quality of both samples for 6 months, keeping the microbial population less than 20 CFU/g. Besides it was not detrimental to the quality attributes, such as instrumental color parameters (L, a, b, ΔE ), browning, TBA value, volatile basic nitrogen, trimethylamine-N, and sensory properties(appearance, color, odor, taste, overall acceptability). However, storage conditions (time, temperature, packing) showed a critical factor influencing the quality changes of dried anchovies. Thermoluminscence analysis was prove to distinguish irradiated from non-irradiated anchovies by comparing both temperature at which glow curves appear and the curve intensity, that was possible even after 6 months at -20℃.

과산화효소를 이용한 백혈구 측정용 뇨 검사지 제조에 관한 연구

송은영,변시명,김희정,김종완,정태화,이홍수,최인성 THE KOREAN SOCIETY FOR BIOMEDICAL LABORATORY SCIEN 1996 Journal of biomedical laboratory sciences Vol.2 No.2

백혈구에 존재하는 과산화 효소를 이용하여 뇨 중 백혈구의 수를 간접적으로 측정하는 새로운 방법의 뇨 검사지를 개발하였다. 과산화 수소원으로서 포도당 산화효소와 포도당을, 색원체로서 tetramethylbenzidine을 처리하여 백혈구 측정용 뇨 검사지를 제조하였으며 검출한계는 10cells/㎕ (5 cells/hpf)이었다. 개발된 백혈구 측정용 뇨 검사지로 뇨를 분석하였을 때 뇨 1/㎕당 10-25cells의 백혈구가 존재할 경우에는 2분내에 연녹색을, 75-250 cells/㎕에서는 녹색을 보였고 500 cells/㎕ 이상에서는 녹청색을 보였다. 172명의 환자뇨를 대상으로 뇨중 백혈구 수치를 현미경 측정 결과와 본 연구에서 개발한 백혈구 측정용 뇨 검사지와 현재 수입 시판 중인 미국의 A사 제품과 B사 제품으로 분석 비교한 결과 좋은 상관관계를 보였다. 이와 같은 평가분석을 통하여 볼 때 본 연구에서 개발한 백혈구 측정용 뇨 검사지는 에스테라아제를 이용한 백혈구 측정용 뇨 검사지와 함께 뇨중 백혈구를 스크리닝 하는데 효과적임을 알 수 있었다. A new test strip to detect leukocytes using the myeloperoxidase in urine was developed. The reagent strip contains tetramethylbenzine, glucose and glucose oxidase. The detection limit was between 10 cells per 1㎕ urine(5 cells/hpf), showing greenish yellow color in the range of 10-25 cells/㎕, green color in the range of 75-250 cells/㎕, greenish blue color in the range of 500 cells/㎕. The result can be obtained within two minute. The performance of the new method was evaluated by comparing the results of microscopic examination and other commercial products. Good correlations were shown between the values obtained by our urine strip and those by other commercial products with 172 urine samples. The results were proven that new methods were useful as primary screening reagents to detect leukocytes in urine.

리튬 이온 2차전지용 Anode 재료의 제조와 그 특성 고찰

이주성,정윤이,변지형,박은성,유덕영 한양대학교 에너지·환경기술연구소 1997 에너지·環境技術論文集 Vol.3 No.-

Carbon materials, disordered carbon and KS6(graphite, Lonza), were studied as anode materials of lithium ion secondary battery. The disordered carbons were made from thermosetting resins (phenol resin, furan resin, acetylene resin and its mixed resin). The resins were carbonized in nitrogen gas at 1000℃. When the thermosetting resins were carbonized, an addition of lithium hydroxide improved capacity and cycle life, but decreased the efficiency of the first charge/discharge. Also, in this study, the capacity of the carbon from phenol resin was higher than that of the carbon from acetylene resin or furan resin. But, the carbon from mixed resin was the highest capacity of all the synthetic carbons.

행정구역 온톨로지를 이용한 관광정보 검색

김영민,정은경,이상준,변영철,도양회 濟州大學校 基礎科學硏究所 2003 基礎科學硏究 Vol.16 No.2

온톨로지를 기반으로 한 정보검색 시스템들은 의미기반 검색을 가능하게 하기 때문에 최근에 많은 연구 개발이 되고 있다. 본 논문에서는 기존 텍스트 기반 정보검색에서 벗어나 실세계 객체들의 관계정보를 이용한 관광정보 검색 시스템을 제안한다. 이를 위해 먼저 제주도내 지역들에 대한 OWL 온톨로지를 설계하고, 이를 기반으로 다양한 광광정보를 검색하는 시스템을 구현한다. 이를통해 제주도내 관광정보에 대하여 사용자가 원하는 정보와 의미적으로 일치하는 결과를 효과적으로 검색할 수 있음을 볼 수 있다. The information retrieval systems based on Ontology are being developed so much because they support the semantic-based information search. In this study we suggest the retrieval system not using text-based tourism informations but using the relation of real world objects. For this, we designed the OWL ontology about the relations of regions in Jeju-Do and the ontology-based retrieval system searching for various tourism informations. Then we found that this system can search the information effectively which the user really want.

비만 여성을 대상으로 한 해조류 추출물의 국부처치와 운동 병행을 통한 지방분해 효과의 임상적 검정

서태수,이인숙,변재철,김규호,박승한,김유영,김상국,김현정,김상기,신동철,성미영,박종석,김종기,박언휘 한국스포츠리서치 2003 한국 스포츠 리서치 Vol.14 No.3

This study was carried out to test the body fat breakdown during topical seaweed extract treatment and in combination with the aerobic exercise. Of 20 female obesity subjects, ten treated a cream types of seaweed extract(control group) and ten treated a placebo cream(placebo group). Topical treatment of each sample was designed for 4 weeks, and treated with 2 times a day about 10ml on the abdomen and the thigh, respectively. All subjects were participated aerobic dance half hour a day and four times a week. After 4 weeks of topical treatment and exercise, they were measured body fat, skinfold thickness, body circumference. Then venous blood samples were taken and analyzed blood cells, serum enzymes, and serum lipids. There were reduction of body weight, % of body fat, antebrachium, brachium, chest, waist, thigh circumference and abdomen thickness between pre and post treatment in both group. Especially, body weight and % of body fat significantly reduced in the subjects treating the seaweed extract than those treating the placebo. There were significant decrement in total cholesterol and LDL whereas increment in HDL and TBIL in the control group, but no significant difference in the placebo group. In conclusion, there were 7.6% of body fat reduction during 4 weeks of aerobic exercise and 21% of body fat reducement in combination of topical seaweed treatment and exercise, respectively. These results suggest that the topical treatment of seaweed extract should be very effective for fat breakdown. Especially, the body fat breakdown maximized in the combination with the topical seaweed extracts treatment and the aerobic exercise.

고콜레스테롤 혈증 환자에서 Cerivastatin(LIPOBAY, 의과학연구소)의 임상효과 및 안전성에 관한 연구

박성훈,김미연,정수진,이영숙,박창한,변은경,전성희 梨花女子大學校 醫科大學 醫科學硏究所 2001 EMJ (Ewha medical journal) Vol.24 No.3

목적 : Cerivastatin(LIPOBAY)은 최근에 개발되어 사용되기 시작한 HMG-CoA 환원효소 억제제로서 저용량으로 탁월한 콜레스테롤 강하효과를 보이는 약물이다. 저자들은 Cerivatatin(LIPOBAY)의 혈중 콜레스테롤 강하효과를 평가하고 약물의 안전성을 평가하기 위하여 본 연구를 시행하였다. 방법 : 고콜레스테롤혈증 환자 37명(남자 13명, 여자 24명)을 대상으로 하였다. 대상환자는 2주간의 저콜레스테롤 식이 후에도 혈청 콜레스테롤이 240㎎/㎗이상이거나, 관상동맥질환이 있는 환자는 혈청콜레스테롤이 220㎎/㎗이상인 경우 cerivastatin 0.4㎎을 8주간 복용하도록 하였다. 임상적 관찰과 검사실 검사(간기능검사, 일반혈액검사, 혈청지질검사)는 투약개시 후 4주 및 8주 후에 시행하였다. 결과 : Cerivastatin 0.4㎎을 4주간 투여한 후 측정한 LDL 콜레스테롤, 총콜레스테롤, triglyceride, 및 HDL 콜레스테롤은 각각 38%, 28.8%, 11.6% 및 7.8% 감소하였다. 총콜레스테롤/HDL 콜레스테롤 비 및 LDL/HDL비는 각각 20.8% 및 31.1% 감소하였다. 투약 8주후에 측정한 검사소견은 4주후에 비해 유의한 변화를 보이지 않았다. 한명의 환자(2.7%)에서 간기능 검사상 AST, 및 ALT의 지속적인 상승으로 투약을 중지하였다. 결론 : Cerivastatin 0.4㎎/day는 혈청콜레스테롤을 효과적으로 감소시켰으며 심각한 부작용은 볼 수 없었다. 따라서 적극적으로 LDL콜레스테롤을 낮출 필요가 있는 고콜레스테롤혈증 환자에서 cerivastatin은 효과적이고 안전한 약물이라고 생각된다. Objectives : Cerivastain(LIPOBAY) is recently developed HMG-CoA reductase inhibitor which is effective in lowering serum cholesterol levels at microgram does. We evaluated the clinical efficacy and safety of cerivastatin(LIPOBAY) in patients with hypercholesterolemia. Method : Thirty-seven patients(male : 13, female : 24) with hypercholesteolemia defined as baseline serum total cholesterol ≥240㎎/㎗, or ≥220㎎/㎗ in patients with known coronary artery disease were included for this study. After 2 weeks of low cholesterol diet, if the serum total choesterol level meet the criteria, cerivastain 0.4㎎/day was prescribed for 8 weeks. Clinical follow-up and laboratory tests were performed 4 weeks and 8 weeks after medication. Results : After 4 weeks of cerivastain 0.4㎎/day treatment, low density lipoprotein(LDL) cholesterol decreased 38% and total cholesterol decreased 28.8% from baseline. Triglyceride decreased 11.6%, and high density lipoprotein(HDL) cholesterol decreased 7.8% from baseline. Total cholesterol/HDL ratio decreased 20.8% and LDL/HDL ratio decreased 31.1% from baseline. After 8 weeks of treatment, no further significant changes were noted compared with the values at 4 weeks. Cervastatin was discontinued in one patient(2.7%) due to continuous liver enzyme elevation. Conclusion : Cerivastatin 0.4㎎/day is effective in lowering serum cholesterol levels without significant adverse reactions. Cerivastatin is effective and safe for patients with hypercholesterolemia who needs aggressive LDL cholesterol lowering.

일부 성장기 학생 혈중 연농도의 성별 및 연령별 분포

임재언,강은용,최병선,서지영,홍연표,장임원,박정덕 중앙대학교 의과대학 의과학연구소 2000 中央醫大誌 Vol.25 No.2