골수염의 진단에 있어서의 Tc-99m Hexamethylpropylene amine oxime 백혈구 스캔의 임상적 유용성

최상호,김양수,정준원,정진원,추은주,서동대,배인규,양승오,우준희,류지소 대한화학요법학회 2001 대한화학요법학회지 Vol.19 No.1

배경 : 골수염의 진단에 있어 Technetium-99m을 이용한 삼상 골스캔(Tc-99m MDP스캔)은 단순방사선 검사상 정상소견을 보이는 경우에 쓰이는 1차적 검사로 매우 높은 민감도를 보인다. 그러나 Tc-99m MDP 스캔은 골절이나, 정형외과적 삽입물, 신경병적 관절병변과 같이 뼈의 재형성이 증가되는 상황에서도 양성소견을 보여 특이도가 낮은 단점이 있다. 이러한 단점을 보완하고자 골수염의 진단에 있어 Tc-99m HMPAO 백혈구 스캔의 유용성을 알아보고자 하였다. 대상 및 방법 : 골수염이 의심되어 Tc-99m HMPAO 백혈구 스캔을 시행했던 15명의 의무기록을 후향적으로 분석하였다. 골수염의 확진은 임상관찰 소견과 배양검사 결과와 병리소견을 기준으로 하였다. 결과 : 골수염의 진단에 있어 Tc-99m HMPAO 백혈구 스캔의 민감도와 특이도는 각각 100%와 78%였고 Tc-99m HMPAO 스캔의 경우는 100%와 18%였다. 가장 흔한 원인균은 methicillin-resistant S. aureus였다. Tc-99m MDP 스캔에서 양성소견을 보였던 환자중 3명의 환자가 Tc-99m HMPAO 백혈구 스캔에서 음성 소견을 보였으며 이 환자들은 추적관찰상 골수염이 없는 것으로 확인되었다. 결론 : Tc-99m HMPAO 백혈구 스캔은 골수염의 존재를 진단하는데 있어 민감도와 특이도가 높으며, 특히 뼈의 재형성이 증가되어 Tc-99m MDP 스캔의 특이도가 낮게 나타나는 상황에서 유용하리라 사료된다. Background Three phase bone scintigraphy, performed with technetium-labeled diphosphonates (Tc-99m MDP scan), is the very sensitive radionuclide procedure of choice for diagnosing osteomyelitis. But, Tc-99m MDP scan is less specific when bone remodeling is increased. To overcome this limitation, we evaluated the diagnostic value of technetium-99m hexamethylpropyleneamine oxime (HMPAO) labeled leukocyte scan in osteomyelitis. Methods : We retrospectively reviewed 15 patients with suspected osteomyelitis who performed Tc-99m HMPAO labeled leukocyte scan. The diagnosis of osteomyelitis was based on clinical course and bacterial culture, and pathologic findings. Results The sensitivity and specificity of Tc-99m HMPAO leukocyte scan was 100% and 78%, and those of Tc-99m MDP scan was 100% and 18%, respectively. The most common organism was Methicilein-Resistant S. aureus. Among the patients that showed persistent accumulation on Tc-99m MDP scan, three patients showed no uptake on Tc-99m HMPAO leukocyte scan and these patients were confirmed that had no osteomyelitis. Conclusion : Tc-99m HMPAO leukocyte scan is a useful test for determining the presence or absence of osteomyelitis, especially under condition that bone remodelin is increased.

알코올성 지방간 유발 마우스에서 다슬기 유래 가수분해물의 간 보호 효과

송은진 ( Eun Jin Song ),조경환 ( Kyoung Hwan Cho ),추호진 ( Ho Jin Choo ),양은영 ( Eun Young Yang ),정윤경 ( Yoon Kyoung Jung ),서민균 ( Min Gyun Seo ),김종철 ( Jong Cheol Kim ),강은주 ( Eun Ju Kang ),류기형 ( Gi Hyung Ryu ),박범 한국산업식품공학회 2017 산업 식품공학 Vol.21 No.4

본 연구는 다슬기의 간 보호 효능을 확인하고자 하였다. 다슬기를 단백질 가수분해효소인 alcalase를 이용하여 가수 분해물을 얻은 후 실험을 수행하였다. HepG2 세포에 다슬기 가수분해물을 처리하였을 때 세포독성이 나타나지 않음을 확인하였다. 그리고 다슬기 가수분해물과 에탄올(1 M)을 가하여 24시간 후 세포생존율을 확인했을 때, 세포가 알코올 독성으로부터 보호되는 것을 확인할 수 있었다. 다음으로 다슬기 가수분해물의 간 손상 보호 효과를 Liber-DeCarli 알코올 액체 식이 급여와 동시에 경구 투여를 통한 알코올 단회 폭음(single binge)으로 알코올성 지방간과 염증을 유발시킨 마우스모델(NIAAA)을 사용하여 확인하고자 하였다. 이들 마우스 모델에서 알코올 식이 급여 후 초기에 체중이 감소하는 경향을 보이나 점차 비알코올 식이 급여군과 유사한 체중으로 회복하였다. 조직학적 관찰에서 알코올로 유도된 간 세포내 지방 축적과 지방성 염증이 에탄올과 함께 다슬기 가수분해물을 투여한 군에서 개선되는 것을 관찰 할 수 있었다. 또한, 알코올 투여에 의한 AST, ALT 및 ALP의 효소활성 증가가 다슬기 가수분해물 처리에 의해 억제되었으며, 만성 알코올 섭취에 의한 염증 매개 효소와 염증성 사인토카인의 발현 증가는 다슬기 가수분해물을 투여한 군에서 양성대조군에 비하여 유의하게 감소하였다. 이상의 연구결과를 종합해 보면 다슬기효소 가수분해물 소재는 알코올성 지방간의 예방 및 개선을 위한 기능성식품용 상업적 소재로서 높은 실용화 가능성을 시사한다. Alcoholic steatosis is a fundamental metabolic disorder and may precede the onset of more severe forms of alco-holic liver disease. In this study, we isolated enzymatichydrolysate from Semisulcospira libertine by alcalase hydrolysis and investigated the protective effect of Semisulcospira libertine hydrolysate on liver injury induced by alcohol in the mouse model of chronic and binge ethanol feeding (NIAAA). In an in vitro study, the hydrolysate protects HepG2 cells from ethanol toxicity. Liver damage was assessed by histopathological examination, as well as by quantitating activities of serum aspartate aminotransferase (AST), alanine aminotransferase (ALT), and alkaline phosphatase (ALP). After the administration of S. libertina hydrolysate, fat accumulation and infiltration of inflammatory cells in liver tissues were significantly decreased in the NIAAA mouse model. The elevated levels of serum AST, ALT, and ALP activities, along with the lipid contents of a damaged liver, were recovered in experimental mice administrated with S. libertina hydrolysate, suggesting its role in blood enzyme activation and lipid content restoration within damaged liver tissues. Moreover, treatment with S. libertine hydrolysate reduced the expression rate of cyclooxygenase (COX-2), interleukin (IL)-1β, and IL-6, which accelerate inflammation and induces tissue damage. All data showed that S. libertine hydrolysate has a preventive role against alcohol-induced liver damages by improving the activities of blood enzymes and modulating the expression of inflammation factor, suggesting S. libertine hydrolysate could be a commercially potential material for the restoration of hepatotoxicity.

Taxonomic Studies of Entomogenous Nematodes of Korea

Young Hak Jung,Eun Ju You,Ho Yul Choo 한국응용곤충학회 2016 한국응용곤충학회 학술대회논문집 Vol.2016 No.04

Various insect pest damage agricultural crops and forest. Several control strategies have been tried against these pests. Using chemical pesticides is one of the major control strategies. However, in recent years, eco-friendly control measures have received a big attention. Entomogenous nematodes are best known as potential biocontrol agent which are promising alternatives of chemical pesticides. During the survey of insect-parasitic nematodes as well as their parasitism in Korea, three new species, Parasitylenchus cyrtogenii n. sp., Parasitylenchus oscavus n. sp., Bathymermis sancheongensis n. sp. and seven other new records for the Korean nematode and nematomorpha fauna were identified, which are: Howardula phyllotretae, Parasitaphelenchus uncinatus, Hexamermis albicans, Hexamermis sp., Heterorhabditidoides chongmingensis, Pristionchus pacificus, and Spinochordodea tellini, respectively. Parasitism of nematodes ranged from 0.7 to 87.5%. The highest parasitism was recorded in P. cyrtogenii n. sp isolated from Cyrtogenius brevior as 87.5%, however the lowest parasitism found in Hexamermis sp. isolated from Oxya chinensis as 0.7%.

정상면역을 가진 환자에서 발생한 복부대동맥의 진균 감염성동맥류 1예

이호영,김태형,추은주,전민혁,이은정,정은정,전성란,박의주,염욱,장원호,황정화,김동훈,김동원 대한감염학회 2008 감염과 화학요법 Vol.40 No.3

감염성동맥류는 드물지만, 치명적인 질환이다. 진성 진균성 감염성동맥류는 더 드물게 발생한다. 진균감염은 사망률과 이환율이 매우 높다. 그러나 증상이 비특이적이고 일반적인 진단방법으로는 민감하게 진단이 되지 않기 때문에 종종 진단이 어렵다. 저자들은 면역이 정상이고 다른 위험인자가 없는 환자에서 복부대동맥에 발생한 진균성 감염성동맥류를 경험하여 이를 보고한다. 컴퓨터 단층촬영과 혈관 수술에 기초하여 진단하였으며, 수술 후 조직배양에서 균이 동정되지는 않았으나 절제한 혈관조직에서 칸디다 감염을 시사하는 발아가 있는 효모균이 관찰되었다. 한국에서 1988년에서 2007년까지 복부에 발생한 감염성동맥류 17증례를 고찰하였을 때 진성 진균성 감염성동맥류는 없었으며, 저자들이 경험한 것이 복부에서 발생한 진균감염성 동맥류로는 첫 증례이다. Infected aneurysms are uncommon, frequently fatal lesions. "True" fungus-infected aneurysms are even rarer. Fungal infections have high morbidity and mortality. However, diagnosis is frequently difficult, since the symptoms are non-specific and standard diagnostic procedures are often insensitive. We experienced a patient with persistent fever and negative blood cultures. The patient was immunocompetent and had no risk factors, and was diagnosed with a fungus-infected aneurysm based on computed tomography and vascular surgery. The vascular tissue revealed some narrow-based budding yeast within the thrombus, suggesting Candida infection. Seventeen cases of infected aneurysm of the abdomen were reported in Korea from 1988 to 2007, although none were "true" fungus-infected aneurysms, making this the first fungus-infected aneurysm of the abdomen in Korea. Prompt diagnostic procedures and aggressive treatment modalities are necessary for patients with occult infection and negative blood cultures, regardless of their immunocompetence, because of the high morbidity and mortality of this condition.

5-Lipoxygenase inhibitors suppress RANKL-induced osteoclast formation via NFATcl expression

( Ju Hee Kang ),( Zheng Ting ),( Mi Ran Moon ),( Jung Seon Sim ),( Jung Min Lee ),( Kyung Eun Doh ),( Sunhye Hong ),( Minghua Cui ),( Sun Choi ),( Hyeun Wook Chang ),( Hea Young Park Choo ),( Mijung Y 영남대학교 약품개발연구소 2016 영남대학교 약품개발연구소 연구업적집 Vol.26 No.-

5-Lipoxygenase synthesizes leukotrienes from arachidonic acid. We developed three novel 5-LO inhibi-tors having a benzoxazole scaffold as a potential anti-osteoclastogenics. They significantly suppressed RANKL-induced osteoclast formation in mouse bone marrow-derived macrophages. Furthermore, one compound. K7, inhibited the bone resorptive activity of osteoclasts. The anti-osteoclastogenic effect of K7 was mainly attributable to reduction in the expression of NFATc1. an essential transcription factor for osteoclast differentiation. K7 inhibited osteoclast formation via ERK and p38 MAPK. as well as NF-KB signaling pathways. K7 reduced lipopolysaccharide (LPS)-induced osteoclast formation in vivo. corroborating the in vitro data. Thus, IG exerted an inhibitory effect on osteoclast formation in vitro and in vivo, properties that make it a potential candidate for the treatment of bone diseases associated with excessive bone resorption.

Pathophysiological Implication of Ganglioside GM3 in Early Mouse Embryonic Development through Apoptosis

Ju Eun-Jin,Kwak Dong-Hoon,Lee Dae-Hoon,Kim Sung-Min,Kim Ji-Su,Kim Sun-Mi,Choi Han-Gil,Jung Kyu-Yong,Lee Seo-ul,Do Su-Il,Park Young-Il,Choo Young-Kug The Pharmaceutical Society of Korea 2005 Archives of Pharmacal Research Vol.28 No.9

Apoptosis may occur in early embryos where the execution of essential developmental events has failed, and gangliosides, sialic acid-conjugated glycosphingolipids, are proposed to regulate cell differentiation and growth. To evaluate the regulatory roles of ganglioside GM3 in early embryonic development, this study examined its expressional patterns in apoptotic cells during early embryonic development in mice. Pre-implanted embryos were obtained by in vitro fertilization, which were treated at the 4-cell stage with three the apoptosis inducers, actinomycin D, camptothecin and cycloheximide, for 15 h. All three inducers significantly increased the percentage of apoptotic cells, as measured using a TUNEL method, but remarkably reduced the total cell numbers. The numbers of morula and blastocyst stages were significantly decreased by treatment of the embryos with the three apoptosis inducers compared with the control, with a similar result also observed in the number of blastomeres. Staining of early embryos with Hoechst 33342 revealed a significant percentage of apoptotic nuclei. Prominent immunofluo­rescence microscopy revealed a significant difference in the ganglioside GM3 expression in apoptotic embryos compared with the control, and RT-PCR also demonstrated a dramatic increase in ganglioside GM3 synthase mRNA in the apoptotic embryos. These results suggest that ganglioside GM3 may be pathophysiologically implicated in the regulation of early embryonic development through an apoptotic mechanism.

Study on Chordodes japonensis and C. fukuii (Nematomorpha: Chordodidae) Parasitism of Tenodera angustipennis and T. aridifolia (Mantodea: Mantidae)

Eun Ju You,Sang Myeong Lee,Young Hak Jung,Jin Young Choi,Ho Yul Choo 한국응용곤충학회 2014 한국응용곤충학회 학술대회논문집 Vol.2014 No.04

Horsehair worm parasitism of mantids was investigated for two years, 2012 and 2013. The mantids were collected from forty-nine sites from August to November. Mantids collected from twenty-one sites (42.9%) including Amur silver grass plantation at Samjang-myeon in Sancheong, Gyeongnam province were parasitized by horsehair worms. C. japonensis was recorded from T. angustipennis and T. aridifolia and C. fukuii, a newly recorded species in Korea, was isolated from T. aridifolia. C. japonensis was found from T. angustipennis and T. aridifolia collected from Gyeongnam province (Jinju, Sancheong, Sacheon, Uiryeong, Hapcheon, and Goseong) and Sangju, Gyeongsbuk province. C. fukuii was isolated from T. aridifolia collected from Gunwi, Gyeongbuk province and Yangpyeong, Gyeonggi province. The C. japonensis parasitism of T. angustipennis and T. aridifolia was 27.0% and 10.7%, respectively. The number of C. japonensis in a host was from one to nine, but most was one from a host. The body length of C. japonensis showed tendency to decrease with number of parasites, but not significantly different. Horsehair worms did not affect body and abdomen length and digestive system of host. However, egg possession of host was different, that is, unparasitized T. angustipennis and T. aridifolia. had more eggs (63.7%) than parasitized ones (2.6%).

Pathophysiological Implication of Ganglioside GM3 in Early Mouse Embryonic Development through Apoptosis

Eun-Jin Ju,곽동훈,Dae-Hoon Lee,Sung-Min Kim,Ji-Su Kim,Sun-Mi Kim,최한길,정규용,이서울,도수일,박용일,Young-Kug Choo 대한약학회 2005 Archives of Pharmacal Research Vol.28 No.9

Apoptosis may occur in early embryos where the execution of essential developmental events has failed, and gangliosides, sialic acid-conjugated glycosphingolipids, are proposed to regulate cell differentiation and growth. To evaluate the regulatory roles of ganglioside GM3 in early embryonic development, this study examined its expressional patterns in apoptotic cells during early embryonic development in mice. Pre-implanted embryos were obtained by in vitro fertilization, which were treated at the 4-cell stage with three the apoptosis inducers, actinomycin D, camptothecin and cycloheximide, for 15 h. All three inducers significantly increased the percentage of apoptotic cells, as measured using a TUNEL method, but remarkably reduced the total cell numbers. The numbers of morula and blastocyst stages were significantly decreased by treatment of the embryos with the three apoptosis inducers compared with the control, with a similar result also observed in the number of blastomeres. Staining of early embryos with Hoechst 33342 revealed a significant percentage of apoptotic nuclei. Prominent immunofluorescence microscopy revealed a significant difference in the ganglioside GM3 expression in apoptotic embryos compared with the control, and RT-PCR also demonstrated a dramatic increase in ganglioside GM3 synthase mRNA in the apoptotic embryos. These results suggest that ganglioside GM3 may be pathophysiologically implicated in the regulation of early embryonic development through an apoptotic mechanism.

반코마이신 내성 장구균 감염에 대한 quinupristin-dalfopristin 치료 경험

추은주,최상호,조영환,정선미,김백남,김남중,김미나,김양수,우준희,류지소 대한화학요법학회 2002 대한화학요법학회지 Vol.20 No.1

VRE 감염이 중환자에서 급속히 증가하고 있으나 이에 대한 확실한 치료제가 없어 새로운 항생제에 대한 활발한 연구가 있는데 그 중 하나가 quinupristin/dalfopristin이고, 저자들은 중증 환자들의 VRE 감염에 대해 quinupristin/dalfopristin를 투여하여 치료한 임상적 경험을 하였기에 문헌고찰과 함께 보고하는 바이다. Vancomycin-resistant enterococci (VRE) was first recognized in 1992 in Korea. VRE infection have been increasingly reported in immunosuppressed patients over the past decade and have become one of major nosocomial pathogens. Clinicians carings for patients with VRE infections face severe constraints in the selection of treatment. Quinupristin/dalfopristin is active in vitro againt vancomycin-resistant E. faecium (VREF), with a MIC_(90) of 1.0㎍/mL. We studied the clinical efficacy and safety of this agent in the treatment of VREF infection. Patients were included if they had signs and symptoms of active infection including bacteremia, intra-abdominal infection, and wound infection. A total of 13 patients with VREF infection were enrolled. A favorable clinical response (cure or improvement) occured in 10 of 11 evaluable patients. The only adverse events related to quinupristin/dalfopristin were arthralgia and myalgia, which occurred in 2 of 13 patients. These results suggest that quinupristin/dalfopristin is effective and safe as treatment for VREF infections in critically ill patients with serious underlying conditions.

Study of the parasitism of Chordodes japonensis and C. fukuii (Nematomorpha: Chordodidae) on Tenodera angustipennis and T. aridifolia (Mantodea: Mantidae)

Eun Ju You,Sang Myung Lee,Young Hak Jung,Jin Young Choi,Ho Yul Choo 한국응용곤충학회 2016 한국응용곤충학회 학술대회논문집 Vol.2016 No.04

Parasitism of horsehair worm in mantids were investigated for two years, from 2012 and 2013. The mantids were collected from forty-nine sites from August to November. Twenty-one sites (42.9%) of the collected mantids were parasitized by horsehair worms. C. japonensis was recorded from T. angustipennis, T. aridifolia and C. fukuii. The C. japonensis parasitism of T. angustipennis and T. aridifolia were 27.0% and 10.7%, respectively. The infested number of C. japonensis was 1-9 per host. The body length of C. japonensis showed tendency to decrease with number of parasites, but not significantly different. Horsehair worms did not affect body, abdomen length and digestive system of host. However, egg possession of host was different, unparasitized T. angustipennis and T. aridifolia had more eggs (63.7%) than parasitized ones (2.6%). C. japonensis was parasitized as immature stage in late Aug. immature and adult stage in early Sept. and only adult in late Oct. Parasitism was the highest in late Sept.