Altered Neuronal Excitability and Synaptic Transmission of Mouse Hippocampus Caused by Neonatal Influenza Infection

Ji Eun Yu,Minsoo Kim,Sung min Kim,Hoyong Park,ChiHye Chung,Sang-Soep Nahm 한국실험동물학회 2014 한국실험동물학회 학술발표대회 논문집 Vol.2014 No.8

Altered Genomic Profile in the Mouse Hippocampus Caused by Neonatal Influenza Infection

Ji Eun Yu,Jin Hee Kim,Chang Seon Song,Sang Soep Nahm 대한수의학회 2015 대한수의학회 학술대회발표집 Vol.2015 No.-

Expression and Distribution of Influenza Virus Receptors in the Various Organs of Wild Birds

Ji Eun Yu,Seok Hoon Lee,Chang Seon Song,Sang Soep Nahm 대한수의학회 2014 대한수의학회 학술대회발표집 Vol.2014 No.-

Pathologic Predictive Factors for Late Recurrence of Hepatocellular Carcinoma in Chronic Hepatitis

( Ji Hae Nahm ),( Hye Sun Lee ),( Ju-seog Lee ),( Haeryoung Kim ),( Jeong Eun Yoo ),( Young Nyun Park ) 대한간학회 2018 춘·추계 학술대회 (KASL) Vol.2018 No.1

Aims: Hepatocellular carcinoma (HCC) has poor outcome, with a cumulative 5-year recurrence rate > 70% even after curative resection. Late recurrence (> 2 years after resection) is regarded as de novo HCC that typically develops from chronic liver disease through a multistep process. It was recently reported that the molecular genetic profile of the background liver rather than HCC itself predicts late HCC recurrence. In this study, we developed and validated a predictive model based on histopathologic features and immunohistochemical marker expression, which is practically useful. Methods: The training (402 cases) and validation (243 cases) cohorts of patients who underwent curative resection for HCC were independently established. Histopathologic features including lobular and porto-periportal inflammatory activity, fibrosis stage, and small or large liver cell changes were evaluated. To evaluate changes in protein expression of genes related to late recurrence, 95 non-tumor liver tissue samples from HCC patients were screened by reverse phase microarray analysis. Expression of phosphorylated signal transducer and activator of transcription (pSTAT)3, phosphorylated extracellular signal-regulated kinase (pERK)1/2, plasminogen activator inhibitor (PAI)-1, and spleen tyrosine kinase (SYK) was detected by immunohistochemical staining. A predictive model was constructed using independent parameters selected by multiple Cox proportional hazards regression analysis. The same analysis was performed in the validation cohort to verify the reliability of the model for predicting late recurrence. Results: In the training cohort, late recurrence of HCC was observed in 74 (18%) patients, with a median follow-up period of 82 months. Late recurrence in HCC patients was correlated with cirrhosis (fibrosis stage 4), small and large liver cell changes, and upregulation of pSTAT3, pERK1/2, and PAI- 1 levels (P<0.05 for all). Cirrhosis (odds ratio [OR] = 2.0; 95% confidence interval [CI]: 1.2-3.2), moderate or severe lobular activity (OR = 21.4; 95% CI: 4.4-104.2), and expression of one or more of pSTAT3, pERK1/2, and SYK (OR = 6.0; 95% CI: 2.0-17.5) as detected by immunohistochemical staining were independently associated with late HCC recurrence according to the multivariate Cox regression analysis (P<0.05 for all). A nomogram was based on these variables to predict late recurrence of HCC, and Harrell’s c index was 0.701 (95% CI: 0.64- 0.75). In the validation cohort, 47 patients (19%) showed late recurrence with a median follow-up period of 56 months and the Harrell’s c index was 0.719 (95% CI: 0.64-0.79) for predicting late recurrence of HCC, demonstrating that the predictive model is highly reliable. Conclusions: Our predictive model based on cirrhosis (fibrosis stage 4), moderate or severe lobular activity, and expression of one or more of pSTAT3, pERK1/2, and SYK in non-tumoral tissue of background liver is useful for predicting late recurrence of HCC after curative resection.

LP9M80-H Isolated from Liriope platyphylla Could Help Alleviate Diabetic Symptoms via the Regulation of Glucose and Lipid Concentration

Ji Eun Kim(김지은),In Sik Hwang(황인식),Jun Seo Goo(구준서),So Hee Nam(남소희),Sun Il Choi(최선일),Hae Ryun Lee(이혜련),Young Ju Lee(이영주),Yoon Han Kim(김윤환),Se Jin Park(박세진),Nahm-Su Kim(김남수),Young Hwan Choi(최영환),Dae You 한국생명과학회 2012 생명과학회지 Vol.22 No.5

LP9M80-H는 맥문동(Liriope platyphylla)으로부터 메탄올과 헥산을 이용하여 추출한 새로운 추출물로서 ICR 마우스에서 인슐린분비를 촉진하며, 간과 뇌 조직에서 인슐린 신호경로를 활성화시키는 것으로 알려져 있다. 본 연구에 서는 LP9M80-H가 당뇨와 비만의 치료에 미치는 효과를 조사하기 위하여, OLETF 모델동물에 LP9M80-H를 2주간 투여한 후 당뇨와 비만과 관련된 주요인자의 변화를 관찰하였다. 비록 체중은 두 집단간에 차이가 없었으나 복부 지방량은 vehicle 투여군보다 LP9M80-H 투여군에서 적었다. 또한, 혈중 포도당농도는 LP9M80-H를 투여한 OLETF랫드가 대조군에 비하여 약간 낮았으나 인슐린의 농도는 유의적으로 크게 증가하였다. 혈청 내 3가지 주요 지질의 농도는 LP9M80-H를 투여한 OLETF 랫드에서 유의적으로 감소하였고, 지방의 산화를 촉진하는 아디포넥틴의 농도도 LP9M80-H를 투여한 OLETF 랫드에서 감소하였다. 더불어, 체내에 분비된 인슐린이 표적장기에 미치는 영향을 관찰하기 위하여 간조직에서 인슐린 수용체와 인슐린 수용체기질(IRS)의 발현을 관찰하였으며, 이러한 2가지 단백질은 LP9M80-H를 투여한 OLETF 랫드에서 vehicle 투여군에 비해 유의미하게 감소하였다. 또한, 인슐린 신호경로의 다운스트림에 위치하는 포도당 수송체 중에서 Glut-2와 Glut-3 발현은 LP9M80-H를 투여한 OLETF 랫드에서 유의미하게 감소하는 반면에, Glut-4 발현은 일정하게 유지되었다. 따라서 이러한 결과는 LP9M80-H는 포도당항상성과 지질농도의 조절을 통하여 당뇨와 비만의 증상을 완화시키는데 기여할 것으로 사료된다. It was reported that the novel compounds (LP9M80-H) of Liriope platyphylla regulate glucose transporter (Glut) biosynthesis by activating the insulin-signaling pathway in the liver and brain of ICR mice. To investigate the therapeutic effects of LP9M80-H on the pathology of diabetes and obesity, alterations of key factors related to symptoms were analyzed in the Otsuka Long Evans Tokushima Fatty (OLETF) rats treated with LP9M80-H for 2 weeks. The abdominal fat masses in the LP9M80-H-treated group were lower than the vehicle-treated group, although there was no difference in body weight between the two groups. Additionally, when compared to the vehicle-treated group, LP9M80-H treatment induced a significant decrease in glucose levels and an increase in the insulin concentration in the blood of OLETF rats. A high level of insulin protein was also detected in pancreatic β cells of LP9M80-H-treated OLETF rats. A significant reduction in the concentration of lipids and adiponectin was detected only in LP9M80-H-treated OLETF rats. Furthermore, the expression of insulin receptor β and the insulin receptor substrate (IRS) was dramatically decreased in LP9M80-H-treated OLETF rats compared to the vehicle-treated group. Of the glucose transporters located downstream of the insulin-signaling pathway, glucose transporters (Glut) -2 and -3 were significantly decreased in LP9M80-H-treated OLETF rats, while the level of Glut-4 was maintained under all conditions. Therefore, these results suggest that LP9M80-H may contribute to relieving symptoms of diabetes and obesity through glucose homeostasis and regulation of lipid concentration.

Unilateral, Single Needle Approach Using an Epidural Catheter for Bilateral Superior Hypogastric Plexus Block

( Ji Seok Baik ),( Eun Joo Choi ),( Pyung Bok Lee ),( Francis Sahngun Nahm ) 대한통증학회 2012 The Korean Journal of Pain Vol.25 No.1

The superior hypogastric plexus block (SHPB) is used for treating pelvic pain, especially in patients with gynecological malignancies. Various approaches to this procedure have been reported due to the anatomic obstacles of a high iliac crest or Large transverse process of the 5th lumbar vertebra. Here, we report a new technique of superior hypogastric plexus block using a unilateral single-needle approach to block the bilateral superior hypogastric plexus with a Tuohy needle and epidural catheter. We have confidence that this new technique can be another option in performing the SHPB when the conventional bilateral approach is difficult to perform. (Korean J Pain 2012; 25: 43-46)

Neonatal influenza infection causes pathological changes in the mouse brain

Yu, Ji Eun,Kim, Minsoo,Lee, Jong-Hwan,Chang, Byung-Joon,Song, Chang-Seon,Nahm, Sang-Soep BioMed Central 2014 VETERINARY RESEARCH Vol.45 No.-

<P>Influenza A virus infections have been proposed to be associated with a broad spectrum of central nervous system complications that range from acute encephalitis/encephalopathy to neuropsychiatric disorders in humans. In order to study early influenza virus exposure in the brain, we created an influenza-infection model in neonatal mice to investigate infection route and resulting pathological changes in the brain. Real-time polymerase chain reaction and immunohistochemical analyses showed that influenza virus infection induced by an intraperitoneal injection was first detected as early as 1 day post infection (dpi), and the peak infection was observed at 5 dpi. The viral antigen was detected in a wide range of brain regions, including: the cerebral cortex, hippocampus, cerebellum, and brainstem. Apoptotic cell death and gliosis were detected in the areas of viral infection. Significant increases in proinflammatory cytokine expression were also observed at 5 dpi. Viral RNAs were detected in the cerebrospinal fluid of infected adult mice as early as 1 dpi. In addition, many infected cells were observed near the ventricles, indicating that the virus may enter the brain parenchyma through the ventricles. These results demonstrate that influenza virus may effectively infect broad regions of the brain through the hematogenous route, potentially through the cerebrospinal fluid along the ventricles, and subsequently induce neuropathological changes in the neonatal mouse brain.</P>

오이 추출물에 존재하는 Superoxide Dismutase의 열안정성

김은애(Eun-Ae Kim),김기남(Gi-Nahm Kim),길지은(Ji-Eun Kil),이민경(Min-Kyung Lee),김석환(Suk-Hwan Kim),서정식(Chung-Sik Suh),박인식(Inshik Park) 한국식품영양과학회 1999 한국식품영양과학회지 Vol.27 No.6

오이속의 조효소액에 존재하는 Superoxide dismutase(SOD)활성의 pH 안정성은 pH 8.0에서 가장 안정하였고 pH5.0~9.0 사이의 범위에서는 비교적 안정하였다. 최적 온도는 25℃였고 열 안정성은 60℃까지는 안정하였다. 100℃에서 5분간 보관하였을 경우에는 12%만이 남아있었다. 오이에 존재하는 SOD 활성이 섭취 후에도 안정한가를 확인하기 위한 실험에서는 위속의 pH와 동일하도록 오이속의 조효소액의 pH를 2.0으로 변형시킨 후 36.7℃에서 3시간 동안 보관 후에 잔존활성이 10%였고, 장내의 환경인 pH 7.0으로 바꾸어 6시간 동안 둔 후 잔존하는 SOD의 활성은 25%로 활성이 증가되었다. 다양한 열처리 후에 잔존하는 오이의 SOD활성은 오이속은 데치기에서(끓는 물에서 2분) 25%, 껍질은 찌는 동안에(3분) 53%, 그리고 속과 껍질로 분리하지 않은 오이는 데치기에서 27%의 활성잔존률을 보였다. 4℃에서는 20일간 보관한 후에 오이속의 조효소액은 81%활성이 있었고, 30℃에서는 17%의 활성이 남아 있었다. 투석한 결과 SOD의 활성은 변화가 없었으므로 오이속에 존재하는 SOD는 적어도 분자량이 12,000 이상의 물질로 추정된다. The superoxide dismutase(SOD) in peeled pericarp of cucumber was most stable at pH 8.0 and relatively stabe between pH 5.0 and 9.0. The enzyme was stable up to 60℃ and retained 12% by heat treatment at 100℃ for 5 min. At pH 2.0, the peeled pericarp enzyme activity was decreased to 10% by incubation for 3 hrs. However, the enzyme activity was increased above 25% after incubating the enzyme at pH 7.0 for 6 hrs. Retention of SOD activity in cucumber by various heating methods was also measured. The residual SOD activities of peeled pericarp and whole cucumber was estimated to be 25% and 27% after blanching (2 min), respectively. The skin enzyme retained 53% of its activity after steaming (3 min). When the peeled peri carp enzyme was incubated at 4℃ for 20 days, the enzyme activity remained about 81%. However, when the enzyme incubated at 30℃ for 20 days, the peeled pericarp enzyme activity decreased to 17% of its original activity. The enzyme activity of peeled pericarp cucumber was not changed after exhaustive dialysis for 3 days, which indicated that the SOD activity in cucumber seems to have molecular weight above 12,000.

자녀의 부정적 정서에 대한 부모의 반응과 아동의 스트레스 대처행동 간의 관계에서 자기조절능력의 매개효과

김지연(Ji Yeon Kim),남은영(Eun Young Nahm) 한국아동학회 2011 아동학회지 Vol.32 No.5

This study explored the relationship between parental reaction to children`s negative emotions, child`s self-regulation and stress-coping behaviors. This study also examined the mediating effect of a child`s self-regulation between parental reactions to children`s negative emotions and a child`s stress-coping behaviors. The sample included 407 elementary school 5th-6th grades and their parents (comprising 407 couples) in Seoul. The research results are summarized as follows. First, parental reactions to children`s negative emotions were significantly related to the child`s self-regulation and stress-coping behaviors. Secondly, it was also found that parental reactions to children`s negative emotion were both partially and indirectly related to a child`s stress-coping behaviors through the child`s use of self-regulation.

Unilateral, Single Needle Approach Using an Epidural Catheter for Bilateral Superior Hypogastric Plexus Block

Baik, Ji-Seok,Choi, Eun-Joo,Lee, Pyung-Bok,Nahm, Francis Sahn-Gun The Korean Pain Society 2012 The Korean Journal of Pain Vol.25 No.1

The superior hypogastric plexus block (SHPB) is used for treating pelvic pain, especially in patients with gynecological malignancies. Various approaches to this procedure have been reported due to the anatomic obstacles of a high iliac crest or large transverse process of the $5^{th}$ lumbar vertebra. Here, we report a new technique of superior hypogastric plexus block using a unilateral single-needle approach to block the bilateral superior hypogastric plexus with a Tuohy needle and epidural catheter. We have confidence that this new technique can be another option in performing the SHPB when the conventional bilateral approach is difficult to perform.