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( Sun Jung Kwon ),( Taeh Yeon Jeon ),( Dong Wook Seo ),( Moon Joon Na ),( Eu Gene Choi,),( Ji Woong Son ),( Eun Hyung Yoo ),( Chang Gyo Park ),( Hoi Young Lee ),( Ju Ock Kim ),( Sun Young Kim ),( Jae 대한결핵 및 호흡기학회 2012 Tuberculosis and Respiratory Diseases Vol.72 No.3
Background: Ventilator-associated pneumonia (VAP) requires prompt and appropriate treatment. Since methicillin- resistant Staphylococcus aureus (MRSA) is a frequent pathogen in VAP, rapid identification of it, is pivotal. Our aim was to evaluate the utility of quantitative polymerase chain reaction (qPCR) as a useful method for etiologic diagnoses of MRSA pneumonia. Methods: We performed qPCR for mecA, S. aureus-specific femA-SA, and S. epidermidis-specific femA-SE genes from bronchoalveolar lavage or bronchial washing samples obtained from clinically-suspected VAP. Molecular identification of MRSA was based on the presence of the mecA and femA-SA gene, with the absence of the femA-SE gene. To compensate for the experimental and clinical conditions, we spiked an internal control in the course of DNA extraction. We estimated number of colony-forming units per mL (CFU/mL) of MRSA samples through a standard curve of a serially-diluted reference MRSA strain. We compared the threshold cycle (Ct) value with the microbiologic results of MRSA. Results: We obtained the mecA gene standard curve, which showed the detection limit of the mecA gene to be 100 fg, which corresponds to a copy number of 30. We chose cut-off Ct values of 27.94 (equivalent to 1×104 CFU/mL) and 21.78 (equivalent to 1×105 CFU/mL). The sensitivity and specificity of our assay were 88.9% and 88.9% respectively, when compared with quantitative cultures. Conclusion: Our results were valuable for diagnosing and identifying pathogens involved in VAP. We believe our modified qPCR is an appropriate tool for the rapid diagnosis of clinical pathogens regarding patients in the intensive care unit.
Kwon, Sun-Jung,Jeon, Tae-Hyeon,Seo, Dong-Wook,Na, Moon-Joon,Choi, Eu-Gene,Son, Ji-Woong,Yoo, Eun-Hyung,Park, Chang-Gyo,Lee, Hoi-Young,Kim, Ju-Ock,Kim, Sun-Young,Kang, Jae-Ku The Korean Academy of Tuberculosis and Respiratory 2012 Tuberculosis and Respiratory Diseases Vol.72 No.3
Background: Ventilator-associated pneumonia (VAP) requires prompt and appropriate treatment. Since methicillin-resistant Staphylococcus aureus (MRSA) is a frequent pathogen in VAP, rapid identification of it, is pivotal. Our aim was to evaluate the utility of quantitative polymerase chain reaction (qPCR) as a useful method for etiologic diagnoses of MRSA pneumonia. Methods: We performed qPCR for mecA, S. aureus-specific femA-SA, and S. epidermidis-specific femA-SE genes from bronchoalveolar lavage or bronchial washing samples obtained from clinically-suspected VAP. Molecular identification of MRSA was based on the presence of the mecA and femA-SA gene, with the absence of the femA-SE gene. To compensate for the experimental and clinical conditions, we spiked an internal control in the course of DNA extraction. We estimated number of colony-forming units per mL (CFU/mL) of MRSA samples through a standard curve of a serially-diluted reference MRSA strain. We compared the threshold cycle (Ct) value with the microbiologic results of MRSA. Results: We obtained the mecA gene standard curve, which showed the detection limit of the mecA gene to be 100 fg, which corresponds to a copy number of 30. We chose cut-off Ct values of 27.94 (equivalent to $1{\times}10^4$ CFU/mL) and 21.78 (equivalent to $1{\times}10^5$ CFU/mL). The sensitivity and specificity of our assay were 88.9% and 88.9% respectively, when compared with quantitative cultures. Conclusion: Our results were valuable for diagnosing and identifying pathogens involved in VAP. We believe our modified qPCR is an appropriate tool for the rapid diagnosis of clinical pathogens regarding patients in the intensive care unit.
스포츠 관전행동의 결정요인에 관한 연구 : 이용자 특성을 중심으로 Focused on Consumer's Psychological Characteristics
박유진,김재휘 한국스포츠심리학회 2003 한국스포츠심리학회지 Vol.14 No.3
본 연구는 스포츠팬의 심리적 특성이 스포츠 관전행동에 영향을 미친다고 가정하고, 스포츠 관전동기와 스포츠팬 정체성의 다양한 차원들 중 스포츠 관전행동을 결정하는 요인들을 밝히고자 하였다. 질문지 조사를 통한 연구 결과 정체성현출성, 친지관계동기, 대리만족동기, 성별의 네 가지 변인이 스포츠 관전행동을 결정하는 요인으로 밝혀졌다. 또한 관전자 특성에 따라 관저행동에 영향을 미치는 관전동기 및 스포츠팬 정체성 요인에 차이가 있는 것으로 나타났다. 즉, 스포츠팬의 성별 및 소극적 관전수준에 따라 관전행동에 영향을 미치는 관전동기 및 스포츠팬 정체성의 구성요소가 달라졌다. 이러한 연구 결과를 스포츠 마케팅에 활용한다면, 관전자 특성에 따라 보다 세분화된 마케팅 전략을 수립할 수 있을 것이다. Watching sport events represents a predominant form of leisure today. Many people attend sport events and think of themselves as sport fans, So, It is needed to understand psychological natures of sports fans and which of psychological natures lead spectators to stadium. Then we could know what makes more steady and devoted fans. Based on previous researches, we could expect that spectator motivation and fan identity would influence on sporting game attendance of spectator. Also we could expect there were sexual differences in effects of motivation and identity factors. To examine these propositions, we performed survey research. Questionnaire of this study included the scales for sports spectator motivation, fan identity dimensions, sport spectating experiences, and demographic variables. Data of this study were collected from 192 college students. The results of the survey analysis verify relationship motive, identity salience, sex, and vicarious satisfaction determine attendance of sporting games. And there were sexual differences in effect of motivation and identity dimensions. These results meant men and women want different benefits through watching sporting events. Therefore, it is needed diverse marketing strategy according to spectators' natures.
Eu Gene Park,Taejun Cho,Keunhee Oh,Soon-Keun Kwon,Dong-Sup Lee,Seung Bum Park,Jaejin Cho The Korean Academy of Oral Biology 2012 International Journal of Oral Biology Vol.37 No.2
The use of high throughput screening (HTS) in drug development is principally for the selection new drug candidates or screening of chemical toxicants. This system minimizes the experimental environment and allows for the screening of candidates at the same time. Umbilical cordderived stem cells have some of the characteristics of fetal stem cell and have several advantages such as the ease with which they can be obtained and lack of ethical issues. To establish a HTS system, optimized conditions that mimic typical cell culture conditions in a minimal space such as 96 well plates are needed for stem cell growth. We have thus established a novel HTS system using human umbilical cord derived-mesenchymal stem cells (hUC-MSCs). To determine the optimal cell number, hUC-MSCs were serially diluted and seeded at 750, 500, 200 and 100 cells per well on 96 well plates. The maintenance efficiencies of these dilutions were compared for 3, 7, 9, and 14 days. The fetal bovine serum (FBS) concentration (20, 10, 5 and 1%) and the cell numbers (750, 500 and 200 cells/well) were compared for 3, 5 and 7 days. In addition, we evaluated the optimal conditions for cell cycle block. These four independent optimization experiments were conducted using an MTT assay. In the results, the optimal conditions for a HTS system using hUCMSCs were determined to be 300 cell/well cultured for 8 days with 1 or 5% FBS. In addition, we demonstrated that the optimal conditions for a cell cycle block in this culture system are 48 hours in the absence of FBS. In addition, we selected four types of novel small molecule candidates using our HTS system which demonstrates the feasibility if using hUC-MSCs for this type of screen. Moreover, the four candidate compounds can be tested for stem cell research application.
Pulmonary Alveolar Proteinosis as an Unusual Pattern of Lung Involvement in Sjogren Syndrome (초)
( Eu Gene Park ),( Hee Jung Kim ),( So Mi Kim ),( Choi Lee ),( Mi Jin Hong ),( Jang Soo Han ),( Jung Hee Park ),( Sung Chul Hong ),( Sang Heon Lee ),( Hae Rim Kim ) 대한내과학회 2010 대한내과학회 추계학술대회 Vol.2010 No.-
Chondrogenesis of human umbilical cord-derived mesenchymal stem cells in vitro by TGFβ3 and BMP6
Eu-Gene Park,조태준,Soon-Keun Kwon,Dong-Sup Lee,조재진 충북대학교 동물의학연구소 2012 Journal of Biomedical and Translational Research Vol.13 No.4
Human umbilical cord is easy to obtain because it is discarded after birth, so it can avoid the ethical issues. Chondrogenesis studies using MSCs from bone marrow, cord blood, adipose has been indicated that TGFβ3 and BMP6 stimulate chondrogenesis. Therefore, we investigated chondrogenesis of the hUC-MSCs on TGFβ3, BMP6 and combination of the two growth factors. We initiated chondrogenesis of the cells by giving physical forces to form 3D cell clusters. After the initiation, we gave four experimental groups for differentiation of the cells as follows; control, 10 ng/mL TGFβ3, 100 ng/mL BMP6, and the combination of 5 ng/mL TGFβ3 and 50 ng/mL BMP6. To analyze of the chondrogenesis, GAG contents, mRNA expression, histological and immunohistochemistry (IHC) were performed. To analyze GAG contents, GAG assay was measured and RT-PCR was performed to determine the chondrogenic markers. Histological analysis was performed through safranin O, alcian blue, and IHC was performed using collagen type I and II. GAG contents were increased that 184% by TGFβ3, 147% by BMP6 and 189% by the combination of TGFβ3 and BMP6 compared to control. The growth factors improved collagen II and aggrecan expression, especially, TGFβ3 and BMP6 were shown synergistic effect compared to only TGFβ3 or BMP6 treated. The histological and IHC analysis indicated that the chondrogenic differentiation in the TGFβ3 and the combination of TGFβ3 and BMP6 showed more cartilage deposition. In conclusion, TGFβ3 and BMP6 differentiated hUC-MSCs into chondrogenic clusters of the combination treatment of the two growth factors showed more efficient chondrogenic ability.