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Han, Hee Dong,Byeon, Yeongseon,Kang, Tae Heung,Jung, In Duk,Lee, Jeong-Won,Shin, Byung Cheol,Lee, Young Joo,Sood, Anil K,Park, Yeong-Min DOVE MEDICAL PRESS 2016 INTERNATIONAL JOURNAL OF NANOMEDICINE Vol.11 No.-
<P>Dendritic cells (DCs) are potent professional antigen-presenting cells that are capable of initiating a primary immune response and activating T cells, and they play a pivotal role in the immune responses of the host to cancer. Prior to antigen presentation, efficient antigen and adjuvant uptake by DCs is necessary to induce their maturation and cytokine generation. Nanoparticles (NPs) are capable of intracellular delivery of both antigen and adjuvant to DCs. Here, we developed an advanced poly(<SMALL>D</SMALL>,<SMALL>L</SMALL>-lactide-co-glycolide) (PLGA)-NP encapsulating both ovalbumin (OVA) as a model antigen and polyinosinic-polycytidylic acid sodium salt (Toll-like receptor 3 ligand) as an adjuvant to increase intracellular delivery and promote DC maturation. The PLGA-NPs were taken up by DCs, and their uptake greatly facilitated major histocompatibility class I antigen presentation in vitro. Moreover, vaccination with PLGA-NP-treated DCs led to the generation of ovalbumin-specific CD8<SUP>+</SUP> T cells, and the resulting antitumor efficacy was significantly increased in EG.7 and TC-1 tumor-bearing mice compared to control mice (<I>P</I><0.01). Taken together, these findings demonstrated that the PLGA-NP platform may be an effective method for delivering tumor-specific antigens or adjuvants to DCs.</P>
이상덕(Sang-Duk Yi),주정현(Jeong-Hyeon Joo),이규희(Gyu-Hee Lee),이기택(K. T. Lee),오만진(Man-Jin Oh) 한국식품영양과학회 2003 한국식품영양과학회지 Vol.32 No.5
밀 단백질에 효소가수분해할 때 생산되는 peptide의 항균활성과 천연항균제로서의 이용가능성을 검토하기 위하여 실험을 행하였다. 밀 단백질에 7종의 단백질가수분해효소를 작용시켜 생성된 가수분해물의 항균활성을 측정하고 한외여과, membrane filtration, HPLC를 이용하여 항균성 peptide를 분리 정제한 후 분자량과 아미노산 결합순서를 측정한 결과는 다음과 같다. 밀 단백질에 7종의 단백질 분해효소를 적용시켜 제조한 가수분해물중 Asp. saito protease를 적용시켜 얻어진 peptide만이 항균활성을 나타내었다. Asp. saito protease는 37℃, pH 6.0에서 작용시킨 경우에 항균활성이 가장 높았으며, 50℃ 이상에서는 활성을 나타내지 않았다. 밀단백 효소가수분해물은 membrane filtration에 의하여 분자량 1,000~3,000에서 항균활성이 나타났다. Membrane filtration으로 얻어진 항균활성분획을 HPLC로 분리한 결과 retention time 31.1~31.8 min에서 항균활성을 나타내었다. 밀단백 효소가수분해물은 121℃에서 15분간 가열하여도 효소활성이 유지되는 매우 안정한 화합물이었다. 항균활성분획을 MALDImass로 질량을 분석한 결과 1,633이었다. 항균성 peptide의 아미노산 결합순서는 cysteine, glycine, prolin, valine, valine, alanine, alanine, arginine의 순서였다. This study was carried out to investigate whether peptide produced from wheat protein by enzyme hydrolysis can be used as a natural antimicrobial agent. Antimicrobial peptide was obtained from wheat protein hydrolyzed by 7 of protease. The produced antimicrobial peptide was purified through ultrafiltration, membrane filtration and HPLC, and molecular weight and amino acid sequence of the purified antimicrobial peptide were determined. Among hydrolysate produced from wheat protein by 7 of protease, antimicrobial activity was observed for the peptide obtained from Asp. saito protease. The Asp. saito protease did produce antimicrobial hydrolysate showing the highest antimicrobial activity at reaction condition of 37℃ and pH 6.0, but not at reaction condition above 50℃. Wheat protein hydrolysate was fractionated by membrane filtration and showed antimicrobial activity between molecular weight 1,000~3,000. The antimicrobial activity fraction obtained by membrane filtration was separated through HPLC and showed antimicrobial activity in the peak of retention time 31.1~31.8 min. We could convince this hydrolysate as heat-stable peptide since antimicrobial activity was maintained after treated with heat for 15 min at 121℃. Molecular weight of antimicrobial peptide identified by MALDI-mass was 1,633. Amino acid sequence of antimicrobial peptide was cysteine, glycine, prolin, prolin, prolin, valine, valine, alanine, alanine and arginine.
Kim, Ga Hee,Won, Ji Eun,Byeon, Yeongseon,Kim, Min Gi,Wi, Tae In,Lee, Jae Myeong,Park, Yun-Yong,Lee, Jeong-Won,Kang, Tae Heung,Jung, In Duk,Shin, Byung Cheol,Ahn, Hyung Jun,Lee, Young Joo,Sood, Anil K. TaylorFrancis 2018 DRUG DELIVERY Vol.25 No.1
<P><B>Abstract</B></P><P>Angiogenesis plays an essential role in the growth and metastasis of tumor cells, and the modulation of angiogenesis can be an effective approach for cancer therapy. We focused on silencing the angiogenic gene PLXDC1 as an important factor for anti-angiogenesis tumor therapy. Herein, we developed PLXDC1 small interfering siRNA (siRNA)-incorporated chitosan nanoparticle (CH-NP/siRNA) coated with hyaluronic acid (HA) to target the CD44 receptor on tumor endothelial cells. This study aimed to improve targeted delivery and enhance therapeutic efficacy for tumor anti-angiogenesis. The HA-CH-NP/siRNA was 200 ± 10 nm in size with a zeta potential of 26.4 mV. The loading efficiency of siRNA to the HA-CH-NP/siRNA was up to 60%. The selective binding of HA-CH-NP/siRNA to CD44-positive tumor endothelial cells increased by 2.1-fold compared with that of the CD44 nontargeted CH-NP/siRNA. PLXDC1 silencing by the HA-CH-NP/siRNA significantly inhibited tumor growth in A2780 tumor-bearing mice compared with that in the control group (<I>p</I> < .01), and mRNA expression of PLXDC1 was significantly reduced in the HA-CH-NP/siRNA-treated group. Furthermore, treatment with HA-CH-NP/siRNA resulted in significant inhibition of cell proliferation (<I>p</I> < .001), reduced microvessel density (<I>p</I> < .001), and increased cell apoptosis (<I>p</I> < .001). This study demonstrates that HA-CH-NP/siRNA is a highly selective delivery platform for siRNA, and has broad potential to be used in anti-angiogenesis tumor therapy.</P>
Guan, Hao-Li,Mandal, P.K.,Lim, Hee-Kyong,Baatartsogt, Oyungerel,Lee, Chi-Ho,Jeon, Gwang-Joo,Choe, Il-Shin,Choi, Kang-Duk Korean Society for Food Science of Animal Resource 2009 한국축산식품학회지 Vol.29 No.2
This study was conducted for the isolation, purification, and characterization of a protease from Korean pear, to see its proteolytic activity on chicken actomyosin and to find the optimum pH and temperature of activity on chicken actomyosin. The protease was isolated from crude extract of Korean pear by ammonium sulfate precipitation. Further purification was done by DEAE-Sepharose ion-exchange chromatography, Mono-Q and Mini-Q column chromatography. The purified enzyme gave a single protein band on SDS polyacrylamide gel electrophoresis and the molecular weight was found to be 38 kDa. The specific activity of purified enzyme was 34,907 unit/mg with 25 fold purification and the yield was 2%. The purified enzyme incubated with chicken actomyosin showed high activity. The optimum pH and temperature for enzyme activity on chicken actomyosin were 6.5 and $70^{\circ}C$, respectively. A protease was purified from Korean pear for the first time and characterized. It was found to be promising for meat tenderization.
Long-Range Lattice Engineering of MoTe<sub>2</sub> by a 2D Electride
Kim, Sera,Song, Seunghyun,Park, Jongho,Yu, Ho Sung,Cho, Suyeon,Kim, Dohyun,Baik, Jaeyoon,Choe, Duk-Hyun,Chang, K. J.,Lee, Young Hee,Kim, Sung Wng,Yang, Heejun American Chemical Society 2017 NANO LETTERS Vol.17 No.6
<P>Doping two-dimensional (2D) semiconductors beyond their degenerate levels provides the opportunity to investigate extreme carrier density-driven superconductivity and phase transition in 2D systems. Chemical functionalization and the ionic gating have achieved the high doping density, but their effective ranges have been limited to similar to 1 nm, which restricts the use of highly doped 2D semiconductors. Here, we report on electron diffusion from the 2D electride [Ca2N](+)e to MoTe2 over a distance of 100 nm from the contact interface, generating an electron doping density higher than 1.6 x 10(14) cm(2) and a lattice symmetry change of MoTe2 as a consequence of the extreme doping. The long-range lattice symmetry change, suggesting a length scale surpassing the depletion width of conventional metalsemiconductor junctions, was a consequence of the low work function (2.6 eV) with highly mobile anionic electron layers of [Ca2N](+)e . The combination of 2D electrides and layered materials yields a novel material design in terms of doping and lattice engineering.</P>
Phase patterning for ohmic homojunction contact in MoTe<sub>2</sub>
Cho, Suyeon,Kim, Sera,Kim, Jung Ho,Zhao, Jiong,Seok, Jinbong,Keum, Dong Hoon,Baik, Jaeyoon,Choe, Duk-Hyun,Chang, K. J.,Suenaga, Kazu,Kim, Sung Wng,Lee, Young Hee,Yang, Heejun American Association for the Advancement of Scienc 2015 Science Vol.349 No.6248
<P><B>Making better contacts</B></P><P>A key issue in fabricating transistors is making a good electrical contact to the semiconductor gate material. For two-dimensional materials, one route is through a phase transition that converts a hexagonally packed semiconductor phase into a distorted octahedrally packed metallic phase. Cho <I>et al.</I> show that laser heating of molybdenum telluride (MoTe<SUB>2</SUB>) achieves this conversion through the creation of Te vacancies. The phase transition improves charge carrier mobility while maintaining the low resistance necessary for improved transistor function.</P><P><I>Science</I>, this issue p. 625</P><P>Artificial van der Waals heterostructures with two-dimensional (2D) atomic crystals are promising as an active channel or as a buffer contact layer for next-generation devices. However, genuine 2D heterostructure devices remain limited because of impurity-involved transfer process and metastable and inhomogeneous heterostructure formation. We used laser-induced phase patterning, a polymorph engineering, to fabricate an ohmic heterophase homojunction between semiconducting hexagonal (2H) and metallic monoclinic (1T’) molybdenum ditelluride (MoTe<SUB>2</SUB>) that is stable up to 300°C and increases the carrier mobility of the MoTe<SUB>2</SUB> transistor by a factor of about 50, while retaining a high on/off current ratio of 10<SUP>6</SUP>. In situ scanning transmission electron microscopy results combined with theoretical calculations reveal that the Te vacancy triggers the local phase transition in MoTe<SUB>2</SUB>, achieving a true 2D device with an ohmic contact.</P>