VOCs의 위해성 평가를 위한 노출분석 방법 연구

조성준,신동천,정용,이덕희,Patrick N. Breysse 한국환경독성학회 2002 환경독성보건학회지 Vol.17 No.2

Volatile organic compounds (VOCs) are an important public health issue in Korea and many important questions remain to be addressed with respect to assessing exposure to these compounds. Because they are ubiquitous and highly volatile, special techniques must be applied in their analytic determination. Valid personal exposure assessment methods are needed to evaluate exposure frequency, duration and intensity, as well as their relationship to personal exposure characteristics. Biological monitoring is also important since it may contribute significantly in risk assessment by allowing the estimation of effective absorbed doses. This study was onducted to establish the environmental measurement, personal dosimetry and biological monitoring methods for VOCs. These methods are needed to compare blood, urinary and exhalation breath VOC levels and to provide tools for risk assessment of VOC exposure. Passive monitors (badge type) and a active samplers (trap) for the VOCs collection were used for air sampling. Methods development included determining the minimum detectable amounts of VOCs in each media, as well as evaluating collection methods and developing analytical procedures. Method reliability was assessed by determining breakthrough volumes and comparing results between laboratories and with other methods. A total capacity of trap used in this study was 60ℓ. Although variable by compound, the average breakthrough was 20%. Also, there was no loss of compounds in trap even if keep for 45 day in -70℃. The recovery of active and passive methods was 69%~126% and method detection limit was 0.24㎍/trap and 0.07㎍/badge. There was no statistical difference (P>0.05) between active and passive methods.

수원시에 대한 4가지 대기분산모델의 상대적 평가연구

박덕신,황인조,김동술 경희대학교 환경연구소 1998 環境硏究 論文集 Vol.8 No.-

Predictions by four atmospheric dispersion models such as TCM, ISCLT, Valley, and TEM were relatively compared in the Suwon City. Each dispersion model was seasonally modified with hourly TSP, SO_(2), NO_(2), and CO ground-level concentration data observed at two telemetered air monitoring sites operated by the Ministry of Environment. Prediction by each model was performed with the same input data prepared by intensive investigation of meteorological data as well as point, area, and line emission inventories in the target area. To present the paired comparison of each model, relative errors among four different models were calculated with reference statistics which were based on the predicted arithmetic means at a number of 48 different sites for each model. Since the main objective in this study was in the relative differences between models, it is impossible to quantitatively determine rank order for models. However, the results by four models show that the maximum relative errors in TSP, SO_(2), NO_(2), and CO concentration were 97.1%, 92.5%, 118.4%, and 49.7%, respectively.

광합성세균 Phodospirillum rubrum의 무기질소원의 이용

강봉조,오덕철,이동헌 濟州大學校 基礎科學硏究所 1997 基礎科學硏究 Vol.10 No.2

Rhodospirillum rubrum, a photosynthetic bacterium, was tested for the utilization of inorganic nitrogen sources such as NH₄Cl, NaNO₂ and NaNO₃. The cell growth was most abundant with NH₄Cl among three nitrogen sources regardless of aerobic or anaerobic condition. With usable nitrogen sources, the cell growth was always greater in anaerobic condition than aerobic condition. R. rubrum used NH₄Cl and NaNO₃, but not NaNO₂ as nitrogen source in anaerobic condition. But in aerobic condition, R. rubrum used all nitrogen sources. The sonsumption of total nitrogen by R. rubrum used was highest in the NH₄Cl medium among three kinds of media regardless of culture conditions. The pH of media were increased according to the cell growth. The production of nitrite from nitrate in the NaNO₃ medium was much greater in anaerobic condition than aerobic condition.

Allylisothiocyanate 첨가가 Aflatoxin 생성 곰팡이 대사산물의 생합성에 미치는 영향

강성조,여명재,이은일,송재영,정덕화 동아시아식생활학회 1996 동아시아식생활학회지 Vol.6 No.1

The effects of allylisothiocyanate on the biosynthesis of various fungus metabolites such as sterigrnatocystin, lipid, protein, citrate RNA and AMP from the culture of Aspergillius parasiticus R-716 were investigated. The content of sterigmatocystin, the precursor of aflatoxin, was lower in the culture added with 50ppm allylisothiocyanate after 48 hours, however was rather higher after 144 hours compared to that of the control. The addition of allylisothiocyanate resulted in the increase of lipid, protein, RNA in mycelium and the content of citrate in the media, but the amount of AMP was low.

근육산소혈행 및 근조성에 영향을 미치는 저항운동의 영향 분석

김덕중,권만근,조병섭 한국스포츠리서치 2004 한국 스포츠 리서치 Vol.15 No.1

The purpose of the present study was to investigate the effects on the capacity of oxygen metabolism and the morphological characteristics in human skeletal muscle by resistance exercise. Seven male restance trainer(trained group) and 6 sedentary male subjects(control group) participated. All subjects experienced muscle biopsy at rest. Also, the muscle oxygenation was measured by near-infrared continuous-wave spectroscopy(NIRcws) during and after squat exercise. It is possible that the muscular resistance exercise lead to hypoxidosis at intramuscle. And a chronic resistance exercise-induced hypoxidosis seems to cause the muscular hypertrophy, especially first-twitch a fiber type(FTa). In addition, the delay of restoration in muscle oxygenation after the squat exercise in trained group may be induced by the hypertrophy of FTa.

제주연안 갯녹음(백화) 지역의 해수에 분포하는 세균군의 분자생물학적 분석

강봉조,김미란,윤병준,이동헌,오덕철,강형일 濟州大學校 基礎科學硏究所 2002 基礎科學硏究 Vol.15 No.2

본 연구에서는 갯녹음(백화)현상이 세균생태계와 어떤 관련이 있는지에 대한 기초자료 및 정보를 얻기 위하여 갯녹음 현상이 일어난 제주도 성산과 강정지역 연안의 해수에 존재하는 세균군을 16S rRNA 증폭기법을 이용하여 조사하였다. 강정지역에서는 Alcanivorax, Paracoccus, Damselae, Pseudomonas, Rhodovulum, Silicibacter, Sulfitobacter, Roseobacter 등 다양한 종류의 세균이 분포되어 있었으며, Alcanivorax가 20%의 빈도로 가장 많이 나타났다. 반면, 성산 지역에서는 Pseudomonas속 균주가 우점종으로서 존재하였으며, Pseudomonas tolaasii(혹은 Pseudomonas corrugata)와 유연관계가 가까운 세균은 44%, Pseudomonas mandeli와 가까운 세균이 24%, Verrucomicrobiales와 가까운 세균은 4%, 기타 동정되지 않은 세균은 세 group으로 구분되었으며, 각각 8%, 8%, 12%를 차지하여, 두 곳에 분포되어 있는 세균군상이 상당한 차이점이 있음을 확인하였다. 갯녹음 지역인 강정과 성산 해수의 8월(표품 채집시기)의 수온은 27℃-27.5℃, 염분의 농도는 30.24-30.60%, pH는 8.23-8.36, 용존산소량(DO)은 각각 7.20-7.28로, 두 지역에서 매우 비슷한 것으로 조사된 바 있는데, 이는 수온이나 염분의 농도, 또는 pH보다는 다른 원인에 의하여 두 갯녹음 지역에 분포하는 세균군의 차이를 가져왔음을 제시해 주었다. In this study, the bacterial communities distributed in sea water of the whitening areas of Gangjeong and Seongsan, Jeju-do have been analyzed using the PCR amplification of 16S rRNA to obtain fundamental data and information on relationship of the whitening phenomenon and microbial ecosystem. In Gangjeong, diverse bacteria such as Alcanivorax, Paracoccus, Damselae, Pseudomonas, Rhodovulum, Silicibacier, Sulfitobacter, and Roseobacier have been found, and Alcanivorax was the most abundant clone. The most abundant clone from Seongsan was Pseudoinonas, of which Pseudomonastolaasii and Pseudonionas mandeli were most abundantly occurred in the frequency of approx 44% and 24%, respectively. Approx 4% of the bacterial clones closest to firruconiicrobiales and other unidentified clones were also found in Seongsan, suggesting there is a great discrepancy between bacterial communities from the whitening areas of Seongsan and Gangjeong. The mean tem- perature, chlorine concentration, pH, and dissolved oxygen (DO) of the sea water of Gangjeong and Seongsan in August of 2001 (sampling period) was 27℃-27.5℃, 30.24-30.60%, pH 8.23-8.36,7.20-7.28 ㎎/ℓ, suggesting other environmental factors except for the factors mentioned above might result in difference of bacterial communities distributed in both areas.

야생너구리(Nyctereutes procyonoides)의 혈액 및 혈액화학치

송근호,유명조,김덕환 충남대학교 수의과대학 동물의과학연구소 2002 動物醫科學硏究誌 Vol.10 No.-

This study was performed to determine the blood and blood chemical values on captured 16 raccoon dogs (Nyctereutes procyonoides). Hematological examination revealed that RBC, Hb, PCV, MCV, MCH and MCHC values were 7.9±0.8x10^(6)/㎕, 14.0±2 g/dl, 44.1±5.5 %, 56.9±3.8 fl, 18.0±1.4 pg and 31.9±2.9 g/dl. respectively, and also revealed that WBC, neutrophil, lymphocyte. eosinophil and monocyte values were 13.6±2.4x10^(3)/㎕, 8.9±2.1x10^(3)/㎕, 2.7±0.8x10^(3)/㎕, 1.1±0.5x10^(3)/㎕ and 1.0±0.4x10^(3)/㎕, respectively. Blood chemical examination showed that total protein, albumin, globulin and A/G ratio values were 7.9±0.9 g/dl, 3.0±0.3 g/dl, 4.8±0.7 g/dl and 0.6±0.1, respectively, and also showed that ALT, AST, BUN. Creatinine and glucose values were 95.8±57.8 1U/ℓ, 64.4±30.1 1U/ℓ, 20.8±5.4 ㎎/dl, 0.7±0.1 ㎎/dl and 58.5±19.1 ㎎/dl, respectively.

고양이 위 평활근에서 KCI에 의한 근수축에 대한 Protein Kinase의 조절

심상수,김승록,심혜선,이덕주,한상준,윤신희,조양혁,김명석 大韓消化器病學會 1998 大韓消化器病學會誌 Vol.32 No.3

고양이 위 평활근 수축에 대한 Vanadate와 Pervanadate의 효과

심상수,최효봉,심혜선,이덕주,윤신희,한상준,김명석,조양혁 大韓消化器病學會 1999 大韓消化器病學會誌 Vol.33 No.1

결핵항원에 의한 결핵환자 말초혈액 단핵구의 IL-2,IL-10 및 TNF-α mRNA 발현비교

박정규,임영재,김화중,조은경,민들레,임재현,최덕례,박성규 충남대학교 의과대학 지역사회의학연구소 1999 충남의대잡지 Vol.26 No.1

The various clinical features of tuberculosis are mediated by diverse cytokines produced by various immune cells which are initially triggered by M. tuberculosis antigens. CD4+ T cells can be classified into two subsets according to the patterns of cytokines they produce; Thl cells give rise to cell-mediated immunity and are characterized by the production of IL-2 and IFN-y, whereas Th2 cells are more efficient in mediating antibody production and secrete II-4, IL-5, IL-6 and II-10, Thl cells can control Th2 cell and vice versa. Peripheral blood mononuclear cells of healthy, cured and chronic refractory tuberculosis patients were stimulated with PPD, TSP and PHA antigen, and lymphoproliferative response and expression of II-2, IL- 10, TNF-α mRNAs were measured. Lymphoproliferative responses to PPD, TSP and PHA antigen were depressed in chronic refractory case compared with others expression of IL-2 mRNA was depressed in chronic refractory case stimulated with all antigens. Expression of IL-10 and TNF-α were depressed in cured and chronic refractory cases stimulated with PPD and TSP antigens.