모기 살충성 세균 B. thuringiensis subsp. israelensis의 효과적인 분리 방법

김광현,이광배,신두만 동의대학교 기초과학연구소 1999 基礎科學硏究論文集 Vol.9 No.1

For more convenient and rapidly isolation of Bacillus thuringiensis subsp. israelensis(Bti), 1) heat treatment for spore forming bacteria, 2) growth in enrichment culture media for Bacillus sp. and 3) selection of bacteria producing a lecithinase for Bacillus thuringiensis subsp. israelensis, were performed. Spore forming bacteria were counted 4.8×10^8cells/g soil on NAPGCY media, 9.2×10^7cells/g soil on NA media, and 3.6×10^8cells/g soil on NAAC media, respectively.; Bacteria producing only a lecithinase were reached at 25.2% on medium contained egg york, bacteria only producing a delta-endotoxin were reached at 23.2% by phase contrast microscope, and bacteria producing a lecithinase & a delta-endotoxin simulten-iously were reached at 13.7%. Bacillus thuringiensis which producing a lecithinase and a delta-endotoxin simultaniously among bacteria producing a lecithinase, were reached at 56.5%; A half of Bacillus thuringiensis was produced a delta-endotoxin, but not produced a lecithinase. Among 8 isolates of Bacillus thuringiensis, two strain of Bti which has a mosquito-cidal toxin, were detected by PCR using a specific primer of δ-endotoxin gene form Bti.

李陸史 硏究 序說

千斗鉉,李充燮 釜山敎育大學 1979 부산교육대학 논문집 Vol.15 No.1

개인 일생의 사적의 기록인 전기란 어떤 하잘 것 없는 사람의 생애에 관한 것일지라도 가식없이 정직하게 이야기된다면 흥미 있는 것이 될 것이다. 그것이 우리가 관심을 갖는 시인의 것이고 또한 그 시인의 창조물인 시의 해석에 유익한 해설을 보태주는 것일때 그 전기는 귀중한 가치를 지니게 된다.

蔚州의 「재밟기(瓦踏)」에 대하여

千斗鉉,李充燮 釜山敎育大學 1975 부산교육대학 논문집 Vol.11 No.1

Jaebabgi is one of the folk recreation transformed from Dabgyo. It is now existing, handed down from the ancient times, in the areas of Gyeonsang do, Jeonlado and chungcheongdo. Among the Jaebabgi plays, Jaebabgi in the Euiseong area and Noddaribabgi in the Andong area are known as the most typical ones. But Wlju's Jaebabgi has been reported for the first time to the academic world by this investigation. Though the plays in the other areas are describing the costumes or the setting of the fable, wlju's play is more Buddhistic and more spectacular in its words. the one who treads on the shoulders of the other pelple is usually a skilled chours leader. It is similar to the Euiseong's Jaebabgi, but it is different from the Euiseong's in the point that the racing or the fighting is not inculded. Two persons with sticks are needed who act as the post men. Some connections with Andong's noddaribabgi have been observed. And also it seems that this play has been trans formed to have some manly aspect under the influence of Kwaejina-ching ching-nane in the point that the refrain follows the chorus leading. This play is one on the fravelling types of play. Because this recreational play has some softness, intensity and the character of play it is considered as possible that this play can be revived and prevailed in the modern times.

모기유충에 대한 살충성 내독소와 항진균성 물질을 동시에 생산하는 B. thuringiensis AF6균주의 분리 및 특성

김광현,이광배,신두만 동의대학교 기초과학연구소 1999 基礎科學硏究論文集 Vol.9 No.1

For a biological control on a plant pathogen, Pyricularia oryzae, and a mosquito, Aedes aegypti, Bacillus thuringiensis strain AF6 which produces parasporal inclusion, delta-endotoxin, was isolated. The B. thuringiensis strain AF6 was produced not only an antifungal substance(AFS) against P. oryzae, but also a mosquitocidal delta-endotoxin. The AFS of the strain AF6 is more stable at pH4.0 than pH10.0. At the mode of action, the AFS of the strain AF6 was inhibited hypha growth on potato agar plate(pH5.0), and degraded cell walls of P. oryzae.

Poster Session : PS 0924 ; Lower GI Tract : Retrospective Clinical Reviews of Abdominal Tuberculosis

( Du Hyeon Lee ),( Hyun Soo Kim ),( Seon Young Park ),( Chang Hwan Park ),( Sung Kyu Choi ),( Jong Sun Rew ) 대한내과학회 2014 대한내과학회 추계학술대회 Vol.2014 No.1

Background: Because the prevalence of tuberculosis is decreasing but that of extrapulmonary tuberculosis is increasing, The objective of this study is to evaluate the clinical features and colonoscopic fi ndings of abdominal tuberculosis. Methods: A retrospective analysis of 130 patients with abdominal tuberculosis who were diagnosed in Chonnam National University Hospital from January 2003 to December 2012 using IBM SPSS Statistics 20. Results: From 2003 to 2012, there are 130 patients of abdominal tuberculosis including 87 patients with tuberculous colitis, 27 patients with tuberculous peritonitis and 16 patients with both. There are 44 male patients (33.8%) and 86 female patients (66.2%). Mean age was 50.2±16.3 (17-92). The number of patients has pulmonary tuberculosis, past pulmonary tuberculosis infection, no known past history of pulmonary tuberculosis was 6(4.6%), 43(33.1%), 81(62.3%) respectively. Symptoms were abdominal pain (64.6%), diarrhea (33.1%), fever (15.4%), weight loss (13.1%), hematochezia (5.4%), and bowel obstruction (3.1%). Asympomatic patients were 17.7%. 16 patients had positive Tuberculin skin test (73%) among 22 patients who were examined Tuberculin skin test. Acid fast bacilli stain was all negative. The positive culture rate of Mycobacterium tuberculosis was 22%. The frequency of positive tuberculosis PCR, positive interferon-gamma release assays was 29.5%, 92.6%, respectively. The colonoscopic involvement sites were terminal ileum and ileocecal area (88.4%), ascending colon (57.3%), transverse colon (14.6%), descending colon (4.9%), rectum and sigmoid colon (8.7%). The percentage of 1 segment involved, 2 segments involved, more than 3 segments involved was 40.8%, 47.5%, 11.7% respectively. Tissue pathologic features were granulomatous change (46.9%), granuloma (7.3%), caseous change (3.1%). Conclusion: Because abdominal tuberculosis does not have specifi c clinical features and low positive ratio of many tuberculosis test, proper diagnosis was done through colonoscopic fi ndings, clinical characteristics and improvement after empirical treatment.

Effects of warming and eutrophication on coastal phytoplankton production

Lee, Kyung Ha,Jeong, Hae Jin,Lee, Kitack,Franks, Peter J.S.,Seong, Kyeong Ah,Lee, Sung Yeon,Lee, Moo Joon,Hyeon Jang, Se,Potvin, Eric,Suk Lim, An,Yoon, Eun Young,Yoo, Yeong Du,Kang, Nam Seon,Kim, Kwan Elsevier Science B.V., Amsterdam. 2019 HARMFUL ALGAE Vol.81 No.-

<P><B>Abstract</B></P> <P>Phytoplankton production in coastal waters influences seafood production and human health and can lead to harmful algal blooms. Water temperature and eutrophication are critical factors affecting phytoplankton production, although the combined effects of warming and nutrient changes on phytoplankton production in coastal waters are not well understood. To address this, phytoplankton production changes in natural waters were investigated using samples collected over eight months, and under 64 different initial conditions, established by combining four different water temperatures (i.e., ambient T, +2, +4, and + 6 °C), and two different nutrient conditions (i.e., non-enriched and enriched). Under the non-enriched conditions, the effect of warming on phytoplankton production was significantly positive in some months, significantly negative in others, or had no effect. However, under enriched conditions, warming affected phytoplankton production positively in all months except one, when the salinity was as low as 6.5. These results suggest that nutrient conditions can alter the effects of warming on phytoplankton production. Of several parameters, the ratio of initial nitrate concentration to chlorophyll <I>a</I> concentration [NCCA, μM (μg L<SUP>−1</SUP>)<SUP>−1</SUP>] was one of the most critical factors determining the directionality of the warming effects. In laboratory experiments, when NCCA in the ambient or nutrient-enriched waters was ≥1.2, warming increased or did not change phytoplankton production with one exception; however, when NCCA was <1.2, warming did not change or decreased production. In the time series data obtained from the coastal waters of four target countries, when NCCA was 1.5 or more, warming increased phytoplankton production, whereas when NCCA was lower than 1.5, warming lowered phytoplankton production, Thus, it is suggested that NCCA could be used as an index for predicting future phytoplankton production changes in coastal waters.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Four temperatures and two nutrient conditions in 8 months were combined. </LI> <LI> Under non-enriched conditions, warming affected positively, negatively, or negligibly. </LI> <LI> Under the enriched conditions, warming in all months except one affected positively. </LI> <LI> Thus, nutrient conditions can alter warming effects on phytoplankton production. </LI> <LI> Ratio of initial nitrate to chlorophyll-a concentration was a critical factor. </LI> </UL> </P>

The Relationship between Restoring force of CBD and Recurrence of CBD stones in elderly patients

( Du Hyeon Lee ),( Seung Dok Hong ),( Han Ra Koh ),( Sung Uk Lim ),( Chung Hwan Jun ),( Chang Hwan Park ) 대한내과학회 2015 대한내과학회 추계학술대회 Vol.2015 No.1

Background and Aims: The risk factors of recurrent CBD stones such as cirrhosis, juxtapapillary diverticulum, gallbladder stone, CBD diameter over 1.5centimeters, endoscopic sphincterotomy and oblique bile duct were well known. But there were no informationabout the relationship between restoring force of common bile duct after ERCP and recurrence of common bile duct stones in elderly patients Patients and Method: In total, 173 elderly patients over 65 years old who underwent 2nd session ERCP within 2 weeks after 1st session ERCP for CBD stone removal were analyzed from January 2006 to December 2010. Results: Out of 173 enrolled patients, 98 (56.6%) were males and 75 (43.4%) females. The age, sex, hypertension, diabetes mellitus, cerebrovascular accident, coronary artery disease, gallbladder stone, liver cirrhosis, previous gastrectomy, intrahepatic bile duct stone and periampullary diverticulum were not different between recurrent group and no recurrent group. But the follow up duration, previous cholecystectomywere significantly different at two groups. In post biliary events, only cholecystitis were different significantly. In recurrent group, mean duration of recurrence was about 19.94 months. Restoring of CBD diameter was defined as more than 3 millimeters of the common bile duct diameter reduction difference between 1st session ERCP and 2nd session ERCP. The diameter of common bile duct was the largest diameter of the common bile duct in cholangiogram. The restoring of CBD diameter was significantly different between recurrent group and no recurrent group. (p value<0.001). But the total number of endoscopic sphincterotomy and laboratory findings including white blood cell, total bilirubin, alkaline phosphatase and alanine transaminase were not different significantly. Conclusions: There was a correlation between restoring force of common bile duct diameter and common bile duct stone recurrence. Therefore We need to close follow up the patients who had not adequate restoring force of common bile duct diameter.

좌전하행지 근위부 스텐트 시술 후 재협착 예측인자

이승현 ( Lee Seung Hyeon ),정명호 ( Jeong Myeong Ho ),심두선 ( Sim Du Seon ),이상현 ( Lee Sang Hyeon ),홍영준 ( Hong Yeong Jun ),박옥영 ( Park Og Yeong ),박우석 ( Park U Seog ),김주한 ( Kim Ju Han ),김인수 ( Kim In Su ),최명자 ( C 대한내과학회 2003 대한내과학회지 Vol.65 No.3

목적 : 좌전하행지 근위부 병변에 대한 관상동맥 스텐트의 치료효과를 분석한 보고는 드문 실정이며, 본 연구에서는 좌전하행지에서 개구부 병변을 제외한 근위부 병변에서 스텐트의 치료 성적을 분석하고, 재협착율, 재협착 예측인자 등을 분석하고자 하였다. 방법 : 1996년부터 2001년까지 전남대학교병원에서 좌전하행지 관상동맥의 개구부 병변 부위를 제외한 근위부 협착 병변이 있어서 스텐트 시술을 받은 후 추적관상동맥을 조영술을 시술받은 환자 412예(남:여= Background : A proximal Left anterior descending coronary artery (LAD) is one of the most important arterial segment due to its wide area of myocardial blood supply and high incidence of restenosis after percutaneous coronary intervention. This study was

원형개구부가 있는 강판 전단벽 시스템을 적용한 학교 건축물의 내진성능평가

이유현(Lee, Yu-Hyeon),이수헌(Lee, Swoo-Heon),이희두(Lee, Hee-Du),신경재(Shin, Kyung-Jae) 대한건축학회 2018 大韓建築學會論文集 : 構造系 Vol.34 No.1

After the Gyeongju earthquake, school buildings were designated as earthquake shelters. However, the ratio of designed for seismic of domestic school buildings is only 23.2% in Korea, and it is necessary to secure the seismic safety of those. Therefore, in this paper, a target building was selected before the seismic design criteria was established and the seismic performance of the building was evaluated. After the evaluation, reinforcement of the building was carried out using seismic retrofit systems which was previously tested. For this purpose, the evaluation was carried out using OpenSees program and the reliability of the seismic retrofit systems was also verified. In this way, we can more precisely reproduce the response of the building in case of actual earthquake and predict damage of the earthquake in the future.

강우유출 모델을 이용한 합류식 하수관거 월류수 저류시설의 용량결정에 관한 연구(2)

이두진(Lee Du Jin),윤현식(Yun Hyeon Sig),선상운(Seon Sang Un),곽수동(Gwag Su Dong),이동훈(Lee Dong Hun) 大韓環境工學會 2004 대한환경공학회지 Vol.26 No.3