Na+/H+ exchanger와 HCO-₃Transporter에 의한 흰쥐 타액선 선세포내 pH 조절

서정택,박동범,손흥규,이종갑 大韓小兒齒科學會 1998 大韓小兒齒科學會誌 Vol.25 No.2

Intracellular pH (pHi) plays an important role in the regulation of cellular processes by influencing the activity of various enzymes in cells. Therefore, almost every type of mammalian cell possesses an ability to regulate its pHi. One of the most prominent mechanisms in the regulation of pHi is Na+/H+ exchanger. This exchanger has been known to be activated when cells are stimulated by the binding of agonist to the muscarinic receptors. Therefore, the aims of this study were to compare the rates of H+ extrusion through Na+/H+ exchanger before and during muscarinic stimulation and to investigate the possible existence of HCO-₃ transporter which is responsible for the continuous supply of HCO-₃ ion to saliva. Acinar cells were isolated from the rat mandibular salivary glands and loaded with pH-sensitive fluoroprobe, 2', 7' -bis(2-carboxyethyl)-5(6)-carboxyfluorescein(BCECF), for 30min at room temperature. Cells were attached onto the coverglass in the perfusion chamber and the changes in pHi were measured on the iverted microscope using spectrofluormeter. 1. By switching the perfusate from HCO-₃-free to HCO-₃-buffered solution, pHi decreased by 0.39±0.02 pH units followed by a slow increase at an initial rate of 0.04±0.007 pH units/min. the rate of pHi increase was reduced to 0.01±0.002 pH units/min by the simultaneous addition of 1 mM amilorede and 100μM DIDS. 2. An addition and removal of NH+₄ caused a decrease in pHi which was followed by an increase in pHi. The increase of pHi was almost completely blocked by 1mM amiloride in HCO-₃-free perfusate which implied that the pHi increase was entired dependent on the activation of Na+/H+ exchanger in HCO-₃=free condition. 3. An addition of 10μM carbachol increased the initial rate of pHi recovery from 0.16±0.01 pH units/min to 0.28±0.03pH units/min. 4. The initial rate of pHi decrease induced by 1mM amilorede was also increased by the exposure of the acinar cells 10μM carbachol(0.06±0.008pH unit/min) compared with that obtained before carbachol sitmulation (0.03±0.004pH unit/min). 5. The intracellular buffering capacity β1 was 14.31±1.82 at pHi 7.2-7.4 and β1 increased as pHi decreased. 6. The rate of H+ extrusion through Na+/H+ exchanger was greatly enhanced by the stimulation of the cells with 10μM carbachol and there was an alkaline shift in the activity of the exchanger. 7. An intrusion mechanism of HCO-₃ was identified in rat mandibular salivary acinar cells. Taken all together, I observed 3-ford increased in Na+/H+ exchanger by the stimulation of the acinar cells with 10μM carbachol at pH 7.25. In addition, I have found an additional mechanism for the regulation of pHi which transported HCO-₃ into the cells.

긴장형 두통환자에서의 근막이완술이 뇌혈류 속도에 미치는 영향

서현규,한종만,이동호 대한정형물리치료학회 2002 대한정형도수물리치료학회지 Vol.8 No.2

The aim of study carried out to determine the effects of myofascial release on the cranial arteries velocity from November 11, 2001 to March 29, 2002 the objects were 10 patients who having the tension-type headache at H-hospital This research compared with measure the mean flow velocity middle cerebral artery, posterior cerebral artery, vertebral cerebral artery. Result obtain were as follows; 1. Middle cerebral artery blood velocity between pre treatment and after treatment for 10days experiment was significantly increased 9.76㎝/s(p<0.05)in right, 4.88㎝/s(p<0.05)in left. 2. Posterior cerebral artery blood velocity between pre treatment and after treatment experiment was difference 6.35㎝/s(p<0.01)in right, 5.14㎝/s(p<0.01)in left, between pre treatment and after treatment for 5days experiment was 11.48㎝/s(p<0.01)in right, 10.74㎝/s(p<0.01)in left, between pre treatment and treatment for 10days experiment was 12.92㎝/s(p<0.001)in right, 12.68㎝/s(p<0.001) in left. 3. Vertebral artery blood velocity between pre treatment and post treatment experiment was difference 4.48㎝/s(p<0.05)in right, 6.10㎝/s(p<0.05)in left, between pre treatment and after treatment for 5days experiment was 12.50㎝/s(p<0.001)in right, 14.40㎝/s(p<0.001)in left, between pre treatment and after treatment for 10days experiment was 14.70㎝/s(p<0.001)in right, 13.90㎝/s(p<0.001)in left.

개에서 발생한 파열된 전이성 비장 혈관육종 1례

서동규,박형진,송근호,서경원 忠南大學校 獸醫科大學 附設 動物醫科學硏究所 2012 動物醫科學硏究誌 Vol.19 No.1

Twelve-year-old, catrated male, weighting 8 kg, Miniature Schnauzer dog was referred to the Veterinary Medical Teaching Hospital of Chungnam National University with history of depression. Result of blood analysis revealed anemia, thrombocytopenia, azotemia, elevated ALP, ALT and CK. On the radiographic examination, splenomegaly and loss of abdominal serosal detail were observed. Abdominal ultrasonography showed rounded mass of spleen and liver, ascites were observed. Splenectomy was performed and found that splentic tail had been ruptured and two samples from nodules on liver were obtained for histopathology. The result of biopsy revealed that hemangiosarcoma of spleen metastasized to the liver. Carboplatin was chosen as the patient had congestive heart failture. During chemotherapy hemangiosarcoma was metastasized into peritoneum and abdominal cavity. Intraperitoneal chemotherapy was preceded at this moment. After 99 days of initial visit, patient was euthanized due to severe anemia and thrombocytopenia.

임신중 산전 초음파로 진단된 자궁근종의 임상적 고찰

서정호,김윤숙,김대원,이동운,최규연,이정재,이임순 순천향의학연구소 2003 Journal of Soonchunhyang Medical Science Vol.9 No.1

Objectives: To study the clinical manifestations; time, mode of delivery, size of myomas and outcome of pregnancy in cases of myomas that were detected prior to pregnancy or during the antenatal care and after delivery. Methods: We statistically analyzed 76 patients with uterine myomas in pregnancy admitted to Soonchunhyang Seoul Hospital during the period of Jan. 1999 and Dec. 2001. Results: The results are as follows. 1. The mean age of patients in the detected group and undetected group were 32.9 years and 32.3 years, respectively. There was no difference in the proportion of primarity between the detected group(98.2%) and the undetected group(79.6%) 2. The most common type of uterine myoma in both groups was intramural myoma (detected group 94.1% vs undetected group 81.3%). The uterine myomas were most commonly located in the anterior portion and fundus(detected group 52.9% and 23.5% respectively vs undetected group 47.4% and 23.7% respectively) and the proportion was not significantly different between the two groups. Uterine myomas with a diameter of 3cm or more in the detected group were detected by clinical examination and ultrasonogram. Rate of uterine myomas with a diameter of 3cm or more in the detected group is 76.5% and less than 3cm is 54.2% which detected incidentally at delivery. 3. The proportion of term infants were detected group 88.2% vs undetected group 91.5%. 4. The most common indications for cesarean section in both groups were cephalopelvic disproportion and repeated cesarean section (detected group 41.2% and 45.8% respectively vs undetected group 11.8% and 18.6% respectively) and there was no significant difference between the two groups. 5. Secondary pathologic changes in the all myomectomy specimens were degeneration in both groups but there was no necrosis or sarcomatous change. 6. Antenatal complications were preterm labor, premature repture of membranes, spontaneous abortion, malpresentation, and placenta previa. Intrauterine growth retardation and placental abruption were not seen in both groups. Conclusion: Myoma detected by ultrasonogram before or during pregnancy is not a significant implication of pregnancy outcome.

어린 개에서 발생한 선천성 특발성 거대도식증 1례

서동규,박형진,송근호,서경원 忠南大學校 獸醫科大學 附設 動物醫科學硏究所 2012 動物醫科學硏究誌 Vol.19 No.1

Three-month-old, intact male, weighting 2.6 kg. Dachshund dog visited to the Veterinary Medical Teaching Hospotal of Chungnam National University with history of regurgitation. Result of blood analysis was in the normal range. On the radiographic examination, diffuse esophageal dilatation was observed. On the esophagram, esophageal dysmotility was observed. Acetylcholine receptor antibody titer was in the normal range. On the endoscopy, esophagitis was observed. Diagnosis was congenital idiopathic megaesophagus. This is the case report of congenital idiopathic megaesophagus.

가열로의 열적해석 및 설계인자에 관한 연구

서용석,강성규,박원희,민동호,김태국 중앙대학교 기술과학연구소 1998 기술과학연구소 논문집 Vol.28 No.-

본 연구에서는 3차원 정육면체 가열로 내의 전도-대류-복사 복합열전달 특서을 해석하였으며, 연소가스의 유입속도, 벽면의 반사율과 매체의 흡수계수 등과 같은 인자들의 변화에 따른 영향을 살펴보았다. 가열로의 벽면들은 단열되어 있고 가열로 내부로 일정한 온도의 연소가스가 유입될 때 시스템 내부의 온도는 유입 온도와 비슷하게 균일한 온도 분포를 갖는 것으로 예측할 수 있었다. 유입 속도가 증가하더라도 가열로 내부의 온도에는 거의 영향을 미치지 않았으며, 가열로는 유입되는 연소가스의 흡수계수가 증가할수록 가열로 내부의 온도는 보다 균일하게 된다. 또한, 가열로 내부 벽의 반사율이 커질수록 벽의 온도는 감소하고 또한 벽면에 작용하는 유효복사열유속은 증가함을 알 수 있었다. 이러한 해석 결과로부터 가열로 내부의 온도 분포는 매체의 흡수계수가 클수록 그리고, 벽면 반사율이 작을수록 일정하게 유지될 수 있다는 것을 알 수 있었다. 그리고 이러한 결과들을 종합하여 볼 때 가열로의 설계 시 일정한 열의 유입과 가열로를 단열시키는 것이 중요하며, 그리고 매체의 흡수 계수를 크게 하고, 그리고 벽면의 반사율을 작게 하는 것은 가열로 내부의 온도를 일정하게 유지할 수 있는 중요한 조건이 된다는 것을 알 수가 있었다.

경부도상 피판을 이용한 구강내 결손부의 재건

양동규,신상훈,서종천,이성근,김종렬 大韓顎顔面成形再建外科學會 1995 Maxillofacial Plastic Reconstructive Surgery Vol.17 No.3

Various cutaneous as well as myocutaneous flaps have been designed for the reconstruction of tissue defects caused by the excision of oral cancer. Among these flaps, cervical island skin flap have been introduced by Farr et al and more have developed by Tashiro et al. This flap has many advantages. The flap minimizes donor size by use of cervical operation wound, flap size available is adequate for most oral defects and the procedure is relatively simple and time saving. However, this flap is not applicable in patients where there are large tissue defects and metastasis is suspected. We used this flap for it's rapid simple, and effective, primary closure of oral defects after cancer ablation and we have found this flap very useful for the reconstruction of relatively small oral defects.

멀티 에이전트를 이용한 지능형 교육 시스템 설계

이동규,서재현 인제대학교 1998 仁濟論叢 Vol.14 No.1

PC의 대중화와 멀티미디어 보편화, 그리고 높은 교육열에 부응하여 PC를 이용한 원격 학습에 대한 관심이 고조되고 있다. 본 논문은 쉽게 이용할 수 있는 PC환경을 기반으로 하여 네트워크를 통해 다수 사용자에게 원격 학습을 제공하고 또한 학습자 수준별 개별 학습으로학습 효과를 극대화하기 위한 지능형 교육 시스템을 제안한다. 제안하는 지능형 교육 시스템은 학습자와 PC의 상호작용으로 학습자의 상태를 진단하고, 개별 학습자 수준을 고려한 동적인 학습과정을 구성하면서 전개해 나간다. 특히 다양한 학습자의 수준 상태에 따른 교육과정을 수행하기 위하여 학습자 모델을 유지관리 한다. 제안하는 지능형 교육 시스템에 필요한 모든 모듈은 에이전트로 대체하여 교육 활동을 효과적으로 수행하도록 제작하였다. With the popularity of PC, universality of multimedia and high-educational -heat, the concerning of remote study using PC is rising. In this paper we propose the ITS(Intelligent Tutoring System) that is able to provide remote study to multi-user through the feasible PC-based network, and maximize study efficiency by private lesson attendant on a learner level. The proposed ITS in this paper is deployed with the diagnosis of learner's state by interaction of learner and his PC, and the construction of dynamic studying course considering private learner level. Especially, this system maintains and administers the learner model for carrying out educational course following various learner's level state. We construct this system to execute efficiently educational activities, replacing its all module which are needed in proposed ITS with agent.

식이 조건에 따른 햄스터의 생장과 조직의 비교

조규태,서동석,안경준 西原大學校 基礎科學硏究所 1999 基礎科學硏究論叢 Vol.13 No.-

식이 조건(pellet, mixed pellet, egg yolk)을 달리하였을 때, 햄스터의 체중 증가량, 먹이 소비량 및 식이 효율 그리고 각 장기의 차이점을 비교하였다. 햄스터의 체중 증가량과 먹이 소비량의 비교는 ANOVA 검정과 Duncan이 다중비교검정에 기초를 두었고 식이 효율은 상관관계를 통하여 알아보았다. 혼합된 사료를 먹었을 때가 가장 많은 체중 증가량을 보였고 다음은 사료, 난황 순서로 증가하였다. 그리고 햄스터는 난황보다는 사료와 혼합 사료를 더 좋아하였으며, 난황만 먹인 경우 간조직에서 병적인 변화를 볼 수 있었다. The increase of weight, consumption rate of food, and histological structure of golden hamster were compared under different condition of feed(pellet, mixed pellet, and egg yolk). The comparison of increasing rate of weight and consumption rate of food was based on ANOVA test and Duncan's multiple range test. The golden hamsters fed on mixed pellet showed prominent growth but in the case of egg yolk showed poor growth. The golden hamsters preferred mixed pellet and pellet to egg yolk, and showed abnormal histological change on the liver and kidney tissue when only the egg yolk was fed.

세리아 안정화 지르코니아 과립분말의 제조 및 소결특성

이종국,서동석,황규홍 조선대학교 생산기술연구소 2000 生産技術硏究 Vol.22 No.1

Stabilized zirconia granule powder was prepared from monoclinic zirconia powder by an addition of ceria and spray-drying method The preparation condition of optimal slurry for nozzle spray-drying was the mixing of 60 vol% zirconia powder containing 12 mol% Ce(NO_3)_3, and 40 % water and milling for 24h in zirconia jar. The slurry was stabilized by the addition of binder (PVA 1Owt%), dispersant(sodium phosphate) 1 wt%, and plasticizer(PEG) 05 wt%. The best spherical granule powder prepared from the condition for the spray-drying is the inlet temperature of 300℃, outlet temperature of 180℃, slurry feeding speed of 850 rpm, and atomizer speed of 40 Hz. The highly dense compact was obtained by the CIP under the pressure of 200 MPa and the sintering at 1500℃ for 2h.