2010 해남, 평창, 원주지역 갈대 등 야초류의 이용실태와 사료가치 평가

서성(Sung Seo),한대덕(Dae Duk Han),장선식(Sun Sik Jang),김원호(Won Ho Kim),정민웅(Min Woong Jung),최진혁(Jin Hyuck Choi),김진숙(Jin Sook Kim),김하영(Ha Young Kim),이종경(Joung Kyong Lee) 한국초지조사료학회 2012 한국초지조사료학회지 Vol.32 No.1

본 연구는 부존 조사료자원 이용으로 수입사료를 줄이고 사료비를 절감하고자 2010년도에 전남 해남(간척지), 강원 평창(강변), 원주(섬강변), 전남 영광(와탄강변) 등 4개 지역에서 갈대와 억새 등 야초 자생지를 탐색하고, 수확·이용실태 조사와 함께 사료가치를 분석하였다. 전남 해남 해남목장 내 갈대(Phragmites communis) 위주의 야초 자생지는 300 ha로 예년에는 2회 곤포작업하여 연간 사일리지로 2,300~2,500톤(ha당 8톤)을 생산하였으나 2010년도는 1회 곤포조제로 사일리지 2,000톤(ha당 7톤)을 생산하였다. 유통가격은 롤 당 53천원으로 ㎏당 110원이었다. 곤포는 전량 자가소비하며, 외부 작업단에 롤 당 26천원의 작업비를 지불하였다. 연간 조수입은 2억 2,260만원이며, 소득은 1억원 수준(345천원/ha)이었다. 초장 150 ㎝의 갈대는 조단백질 8.4%, 상대사료가치 71.9 (건초 5등급), 건물 소화율 60.1%로 사료가치는 볏짚에 비해 약간 나은 것으로 평가되었다. 갈대 사일리지의 사료가치와 유기산 함량은 동일 지역에서 동일한 재료로 사일리지를 조제하더라도 수확시기에 따라 차이가 컸으며(CP 4.7~6.4%, RFV 62.2~78.9, 건물 소화율 41.9~53.9%), 갈대 사일리지의 품질은 수단그라스에 비해 불량하였다. 강원 평창 강변 자생갈대의 사료가치는 초장이 79, 117, 121, 142㎝로 생육이 진행됨에 따라 낮아져, 조단백질은 각각 13.9%에서 7.6%로, 상대사료가치는 90.7 (건초 3등급)에서 76.1 (건초 4등급)로, 건물 소화율은 72.9%에서 54.7%로 감소하였다. 원주 섬강 주변 야초류는 곤포수확 작업에 어려움이 있었으며, 초장 130 cm의 갈대는 조단백질 8.5%, 상대사료가치 82.3 (건초 4등급), 건물 소화율 70.2%로 사료가치는 높았으며, 220㎝의 성숙한 갈대는 조단백질 10.2%, 상대사료가치 65.1 (건초 5등급), 건물 소화율 48.9%로 볏짚 대비 조금 나은 것으로 평가되었다. 전남영광지역에서는 와탄천 주변에 자생하는 갈대를 수거 이용하고자 시도하였으나 경제성이 없어 중단하였다. 이상의 결과를 종합하여볼 때, 갈대, 억새 등 야초류는 현 상태에서 최대한 수거·이용하되, 사료가치를 유지하고 가축 기호성이 양호한 시기에 수확하는 것이 중요하며, 최소한 볏짚과 비슷하거나 그 이상의 사료가치를 기대하기 위해서는 7월 중순 이전(늦어도 7월까지)에 수확·이용하는 것이 권장된다. This study was carried out in 2010 to investigate the utilization and forage quality of native grasses, such as Phragmites communis which might reduce the cost of feeding domestic cattle. The regions surveyed were the Haenam ranch in Haenam, Pyeongchang, Wonju, and Yeonggwang. In Haenam, yearly silage production harvested from 300 ha was 2,000 MT (7 MT/ha). All of those round bale silages were self-consumed in that region, and marketing price was 50,000 55,000 won per roll (110 won/㎏). Phragmites communis of 150 ㎝ in length contained 8.4% crude protein (CP) with relative feed value (RFV) 71.9 and 60.1% in vitro dry matter digestibility (IVDMD), which was somewhat more favorable than forage quality of rice straw. The silage quality of Phragmites communis varied greatly by the time of harvest (CP 4.7 6.4%, RFV 62.2 78.9, and IVDMD 41.9 53.9%), even with the same district and of the same original forage materials. Generally, the quality of Phragmites communis silage of was poorer than that of sorghum * sudangrass hybrid. In Pyeongchang, forage quality of Phragmites communis was decreased from 13.9% to 7.6% in CP, 90.7 to 76.1 in RFV, and 72.9% to 54.7% in IVDMD, as plant was getting mature, from 79 ㎝ to 117 ㎝, 121 ㎝ or to 142 ㎝ in length. In Wonju, the quality values of Phragmites communis of 130 ㎝ in length were 8.5% CP, 82.3 RFV and 70.2% IVDMD, while those of matured grasses of 220 ㎝ in length were lower (10.2% CP, 65.1 RFV and 48.9% IVDMD), but this was a little more favorable than quality of rice straw. In Yeonggwang, feeding Phragmites communis was tried in a Hanwoo feed, but stopped due to low profitability. In conclusion, the overall quality of most native grasses including Phragmites communis in this survey was poor. Therefore, we recommend that Phragmites communis and native grasses should be harvested on June or July to obtain richer forage quality in forage values than rice straw.

우리나라 노인여성의 골다공증성 척추골절로 인한 경제적 부담 추계

강혜영,강대룡,장영화,박성은,최원정,문성환,양규현,Kang, Hye-Young,Kang, Dae-Ryong,Jang, Young-Hwa,Park, Sung-Eun,Choi, Won-Jung,Moon, Seong-Hwan,Yang, Kyu-Hyun 대한예방의학회 2008 예방의학회지 Vol.41 No.5

Objectives : To estimate the economic burden of osteoporotic vertebral fracture (VF) from a societal perspective. Methods : From 2002 to 2004, we identified all National Health Insurance claims records for women ${\geq}50$ years old with a diagnosis of VF. The first 6-months was defined as a "clearance period," such that patients were considered as incident cases if their first claim of fracture was recorded after June 30, 2002. We only included patients with ${\geq}$ one claim of a diagnosis of, or prescription for, osteoporosis over 3 years. For each patient, we cumulated the claims amount for the first visit and for the follow-up treatments for 1 year. The hospital charge data from 4 hospitals were investigated to measure the proportion of the non-covered services. Face-to-face interviews were conducted with 106 patients from the 4 study sites to measure the out-of-pocket spending outside of hospitals. Results : During 2.5 years, 131,453 VF patients were identified. The patients had an average of 3.38 visits, 0.40 admissions and 6.36 inpatient days. The per capita cost was 1,909,690 Won: 71.5% for direct medical costs, 20.6% for direct non-medical costs and 7.9% for indirect costs. The per capita cost increased with increasing age: 1,848,078 Won for those aged 50-64, 2,084,846 Won for 65-74, 2,129,530 Won for 75-84and 2,121,492 Won for those above 84. Conclusions : Exploring the economic burden of osteoporotic VF is expected to motivate to adopt effective treatment options for osteoporosis in order to prevent the incidence of fracture and the consequent costs.

APP Tail 1 (PAT1) Interacts with Kinesin Light Chains (KLCs) through the Tetratricopeptide Repeat (TPR) Domain

Won Hee Jang(장원희),Sang-Jin Kim(김상진),Young Joo Jeong(정영주),Hee Jae Jun(전희재),Il Soo Moon(문일수),Dae-Hyun Seog(석대현) 한국생명과학회 2012 생명과학회지 Vol.22 No.12

KIF5/Kinesin-I는 경쇄(light chain)를 통하여 결합함으로써 다양한 운반체들을 미세소관을 따라 운반한다. Kinesin light chains (KLCs)은 tetratricopeptide repeat (TPR) 영역을 매개로 운반체와 결합한다. 현재까지 KLCs와 결합하는 많은 운반체들이 확인되었으나 KLCs가 어떻게 특정운반체를 인식하여 결합하는지는 아직 확실히 밝혀지지 않았다. 본 연구에서 KLC1의 TPR 영역과 결합하는 단백질을 분리하기 위하여 효모 two-hybrid system을 이용하여 탐색한 결과 amyloid precursor protein (APP)과 결합하는 것으로 보고된 protein interacting with APP tail 1 (PAT1)을 분리하였다. KLC1은 PAT1의 C-말단 부위와 결합하며, PAT1은 KLC1의 TPR 영역을 포함한 부위와 결합함을 효모 two-hybrid assay로 확인하였다. 또한 PAT1는 KLC2와도 결합하였지만 kinesin heavy chains (KHCs)인 KIF5A, KIF5B, KIF5C와는 결합하지 않았다. 단백질간 결합은 glutathione S-transferase (GST) pull-down assay와 공동면역침강으로도 확인하였다. 생쥐의 뇌 파쇄액을 PAT1 항체와 APP 항체로 면역침강을 행한 결과 KLC와 KHCs가 같이 침강하였다. 이러한 결과들은 PAT1이 Kinesin-I와 APP 포함 소포간의 상호작용을 매개한다는 것을 시사한다. A conventional kinesin, KIF5/Kinesin-I, transports various cargoes along the microtubule through interaction between its light chain subunit and the cargoes. Kinesin light chains (KLCs) interact with many different cargoes using their tetratricopeptide repeat (TPR) domain, but the mechanism underlying recognition and binding of a specific cargo has not yet been completely elucidated. We used the yeast two-hybrid assay to identify proteins that interact with the TPR domain of KLC1. We found an interaction between the TPR domain of KLC1 and an amyloid precursor protein (APP)-binding protein PAT1 (protein interacting with APP tail 1). The yeast two-hybrid assay demonstrated that the TPR domain-containing region of KLC1 mediated binding to the C-terminal tail region of PAT1. PAT1 also bound to KLC2 but not to kinesin heavy chains (KIF5A, KIF5B, and KIF5C) in the yeast two-hybrid assay. These protein-protein interactions were also observed in the glutathione S-transferase (GST) pull-down assay and by co-immunoprecipitation. Anti-PAT1 antibody as well as anti-APP antibody co-immunoprecipitated KLC and KHCs associated with PAT1 from mouse brain extracts. These results suggest that PAT1 could mediate interactions between Kinesin-I and APP containing vesicles.

Surface plasmon resonance based detection of single-walled carbon nanotube using DNAs

Won-Hwi Na,Dae-Ho Jang,Min-Wook Kang,Se-Hyun Shin 대한기계학회 2014 대한기계학회 춘추학술대회 Vol.2014 No.11

Single-walled carbon nanotubes (SWNTs) consist of carbon atoms are pipe-shaped nano materials with a diameter of 10nm or less. Because SWNTs have outstanding mechanical, optical and electrical properties, they are widely used in industry. Recent studies have reported the toxicity of SWNTs. Toxicity assessment must take precedence in order to prevent environmental pollution. We proposed surface plasmon resonance based detection of single-walled carbon nanotube using DNAs. In this study, r-ssDNA (receptor single strand DNA) was immobilized on the sensor chip surface. SWNTs are wrapped by L1-ssDNA having complementary sequence of immobilized r-ssDNA. To enhance the sensitivity, L1-ssDNA SWNTs were aggregated via L2-ssDNA. The L1-ssDNA wrapping SWNTs bind to the r-ssDNA immobilized on sensor chip surface. Through this method, we effectively detect SWNT SPR sensor by 100pg/ml.

Rapid Synthesis of Magnetite Catalysts Incorporated with Metal Promoters for High Temperature Water Gas Shift Reaction

( Dae-woon Jeong ),( Won-bi Han ),( Jae-oh Shim ),( Won-jun Jang ),( Yeol-lim Lee ),( Hyun-seog Roh ),( Jae Hoi Gu ),( Yong Taek Lim ) 한국폐기물자원순환학회(구 한국폐기물학회) 2014 한국폐기물자원순환학회 심포지움 Vol.2014 No.1

A rapid one step direct synthesis of high temperature water gas shift (WGS) catalysts, magnetite incorporated with various promoters like Co, Ni, Cu, and Zn have been shown. An amino acid glycine was used as a complexing agent as well as a fuel for the auto-combustion process. The zwitterionic nature of glycine favors the easy incorporation of the promoter metal ions. In addition, reducing atmosphere created by glycine during its burning makes it possible to prepare magnetite directly. During the high temperature WGS, the activity follows the order Cu-FAG > Ni-FAG > Zn-FAG > Co-FAG. The optimization of Cu loading is also carried out. At 12.5 mole % of Cu loading the catalytic activity reached a CO conversion value of 87% at 400℃.

Geometric Sensitivity Index for the GNSS Using Inner Products of Line of Sight Vectors

Dae Hee Won,Jongsun Ahn,Sangkyung Sung,Chulsoo Lee,Sungchun Bu,Jeagyu Jang,Young Jae Lee 한국항공우주학회 2015 International Journal of Aeronautical and Space Sc Vol.16 No.3

Satellite selection and exclusion techniques have been applied to the global navigation satellite system (GNSS) with the aim of achieving a balance between navigational performance and computational efficiency. Conventional approaches to satellite selection based on the best dilution of precision (DOP) are excessively computational and complicated. This paper proposes a new method that applies a geometric sensitivity index of individual GNSS satellites. The sensitivity index is derived using the inner product of the line of sight (LOS) vector of each satellite. First, the LOS vector is computed, which accounts for the geometry between the satellite and user positions. Second, the inner product of each pair of LOS vectors is calculated, which indicates the proximities of the satellites to one another. The proximity can be determined according to the sensitivity of each satellite. A post-processing test was conducted to verify the reliability of the proposed method. The proposed index and the results of a conventional approach that measures the dilution of precision (DOP) were compared. The test results demonstrate that the proposed index produces results that are within 96% of those of the conventional approach and reduces the computational burden. This index can be utilized to estimate the sensitivity of individual satellites, obtaining a navigation solution. Therefore, the proposed index applies to satellite selection and exclusion as well as to the sensitivity analyses of multiple GNSS applications.

Thermopiezoelectric Cantilever for Probe-Based Data Storage System

Jang, Seong-Soo,Jin, Won-Hyeog,Kim, Young-Sik,Cho, Il-Joo,Lee, Dae-Sung,Nam, Hyo-Jin,Bu, Jong. U. The Institute of Electronics and Information Engin 2006 Journal of semiconductor technology and science Vol.6 No.4

Thermopiezoelectric method, using poly silicon heater and a piezoelectric sensor, was proposed for writing and reading in a probe based data storage system. Resistively heated tip writes data bits while scanning over a polymer media and piezoelectric sensor reads data bits from the self-generated charges induced by the deflection of the cantilever. 34${\times}$34 array of thermopiezoelectric nitride cantilevers were fabricated by a single step wafer level transfer method. We analyzed the noise level of the charge amplifier and measured the noise signal. With the sensor and the charge amplifier 20mn of deflection could be detected at a frequency of 10KHz. Reading signal was obtained from the cantilever array and the sensitivity was calculated.

Investigation of Optical and Structural Stability of Localized Surface Plasmon Mediated Light‐Emitting Diodes by Ag and Ag/SiO₂ Nanoparticles

Jang, Lee‐,Woon,Jeon, Dae‐,Woo,Kim, Myoung,Jeon, Ju‐,Won,Polyakov, Alexander Y.,Ju, Jin‐,Woo,Lee, Seung‐,Jae,Baek, Jong‐,Hyeob,Yang, Jin‐,Kyu,Lee, In‐,H WILEY‐VCH Verlag 2012 Advanced functional materials Vol.22 No.13

<P><B>Abstract</B></P><P>Localized surface plasmon (LSP) effects due to Ag and Ag/SiO<SUB>2</SUB> nanoparticles (NPs) deposited on GaN/InGaN multiquantum well (MQW) light‐emitting diode (LED) structures are studied. The colloidal NPs are synthesized by a sol‐gel method and drop‐cased on the LED structures. The surface density of NPs its controlled by the concentration of the NP solution. Theoretical modeling is performed for the emission spectrum and the electric field distribution of LSP resonance for Ag/SiO<SUB>2</SUB> NPs. Enhanced photoluminescence (PL) efficiency is observed in the LED structures and the amount of PL enhancement increases with increasing the surface density of Ag and Ag/SiO<SUB>2</SUB> NPs. These effects are attributed to resonance coupling between the MQW and LSP in the NPs. It is also shown that the PL enhancement attainable with Ag NPs and Ag/SiO<SUB>2</SUB> NPs is comparable, but the latter displays a much higher stability with respect to long‐term storage and annealing due to a barrier for NP agglomeration, Ag oxidation, and impurity diffusion provided by the SiO<SUB>2</SUB> shell.</P>

Molecular Gene Cloning, Expression, and Characterization of Bovine Brain Glutamate Dehydrogenase

( Dae Won Kim ),( Won Sik Eum ),( Sang Ho Jang ),( Chang Sik Yoon ),( Young Hoon Kim ),( Soo Hyun Choi ),( Hee Soon Choi ),( So Young Kim ),( Hyeok Yil Kwon ),( Jung Hoon Kang ),( Oh Shin Kwon ),( Sun 생화학분자생물학회 2003 BMB Reports Vol.36 No.6

A cDNA of bovine brain glutamate dehydrogenase (GDH) was isolated from a cDNA library by recombinant PCR. The isolated cDNA has an open-reading frame of 1677 nucleotides, which codes for 559 amino acids. The expression of the recombinant bovine brain GDH enzyme was achieved in E. coli, BL21 (DE3) by using the pET-15b expression vector containing a T7 promoter. The recombinant GDH protein was also purified and characterized. The amino acid sequence was found 90% homologous to the human GDH. The molecular mass of the expressed GDH enzyme was estimated as 50 kDa by SDS-PAGE and Western blot using monoclonal antibodies against bovine brain GDH. The kinetic parameters of the expressed recombinant GDH enzymes were quite similar to those of the purified bovine brain GDH. The L_(nm) and V_(max) values for NAD^(+) were 0.1 mM and 1.08 μmol/min/mg, respectively. The catalytic activities of the recombinant GDH enzymes were inhibited by ATP in a concentration-dependent manner over the range of 10 - 100 μM, whereas, ADP increased the enzyme activity up to 2.3-fold. These results indicate that the recombinant-expressed bovine brain GDH that is produced has biochemical properties that are very similar to those of the purified GDH enzyme.

Poster Session 1 : Mutation of the human MODY gene in Korean patient with diabetes

( Dae Yong Park ),( Sang Bum Park ),( Youn Hyoung Nam ),( Jae Sik Lee ),( Won Kang ),( Sang Hyun Lee ),( Won Cheoul Jang ),( Jee Suk Yu ) 한국생화학분자생물학회 (구 한국생화학회) 2005 생화학분자생물학회 춘계학술발표논문집 Vol.2005 No.-