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Ahn, Dae-Gyun,Choi, Jin-Kyu,Taylor, Deborah R.,Oh, Jong-Won Springer-Verlag 2012 Archives of virology Vol.157 No.11
<P>The severe acute respiratory syndrome coronavirus (SARS-CoV) RNA genome is replicated by a virus-encoded RNA replicase, the key component of which is the nonstructural protein 12 (nsp12). In this report, we describe the biochemical properties of a full-length recombinant SARS-CoV nsp12 RNA-dependent RNA polymerase (RdRp) capable of copying viral RNA templates. The purified SARS-CoV nsp12 showed both primer-dependent and primer-independent RNA synthesis activities using homopolymeric RNA templates. The RdRp activity was strictly dependent on Mn(2+). The nsp12 preferentially copied homopolymeric pyrimidine RNA templates in the absence of an added oligonucleotide primer. It was also able to initiate de novo RNA synthesis from the 3'-ends of both the plus- and minus-strand genome of SARS-CoV, using the 3'-terminal 36- and 37-nt RNA, respectively. The in vitro RdRp assay system established with a full-length nsp12 will be useful for understanding the mechanisms of coronavirus replication and for the development of anti-SARS-CoV agents.</P>
( Dae-gyun Ahn ),( Hye-jin Shin ),( Mi-hwa Kim ),( Sunhee Lee ),( Hae-soo Kim ),( Jinjong Myoung ),( Bum-tae Kim ),( Seong-jun Kim ) 한국미생물 · 생명공학회 2020 Journal of microbiology and biotechnology Vol.30 No.3
Coronavirus disease 2019 (COVID-19), which causes serious respiratory illness such as pneumonia and lung failure, was first reported in Wuhan, the capital of Hubei, China. The etiological agent of COVID-19 has been confirmed as a novel coronavirus, now known as severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), which is most likely originated from zoonotic coronaviruses, like SARS-CoV, which emerged in 2002. Within a few months of the first report, SARS-CoV-2 had spread across China and worldwide, reaching a pandemic level. As COVID-19 has triggered enormous human casualties and serious economic loss posing global threat, an understanding of the ongoing situation and the development of strategies to contain the virus’s spread are urgently needed. Currently, various diagnostic kits to test for COVID-19 are available and several repurposing therapeutics for COVID-19 have shown to be clinically effective. In addition, global institutions and companies have begun to develop vaccines for the prevention of COVID-19. Here, we review the current status of epidemiology, diagnosis, treatment, and vaccine development for COVID-19.
RNA aptamer-based sensitive detection of SARS coronavirus nucleocapsid protein
Ahn, Dae-Gyun,Jeon, Il-Ji,Kim, Jung Dong,Song, Min-Sun,Han, Seung-Ryul,Lee, Seong-Wook,Jung, Hyungil,Oh, Jong-Won Royal Society of Chemistry 2009 The Analyst Vol.134 No.9
<P>Severe acute respiratory syndrome coronavirus (SARS-CoV) is the etiological agent of a newly emerged disease SARS. The SARS-CoV nucleocapsid (N) protein is one of the most abundant structural proteins and serves as a diagnostic marker for accurate and sensitive detection of the virus. Using a SELEX (systematic evolution of ligand by exponential enrichment) procedure and recombinant N protein, we selected a high-affinity RNA aptamer capable of binding to N protein with a dissociation constant of 1.65 nM. Electrophoretic mobility shift assays and RNA competition experiments showed that the selected aptamer recognized selectively the C-terminal region of N protein with high specificity. Using a chemiluminescence immunosorbent assay and a nanoarray aptamer chip with the selected aptamer as an antigen-capturing agent, we could sensitively detect N protein at a concentration as low as 2 pg/ml. These aptamer–antibody hybrid immunoassays may be useful for rapid, sensitive detection of SARS-CoV N protein.</P> <P>Graphic Abstract</P><P>We describe the high-affinity RNA aptamers capable of capturing SARS coronavirus (SARS-CoV) nucleocapsid (N) protein. A nanoarray aptamer chip with the selected aptamer might have application potential for rapid, sensitive detection of SARS coronavirus. <IMG SRC='http://pubs.rsc.org/services/images/RSCpubs.ePlatform.Service.FreeContent.ImageService.svc/ImageService/image/GA?id=b906788d'> </P>
안대균(Ahn Dae Gyun),우은택(Woo Eun Taek),조윤현(Cho Yun Hyun),한승호(Han Seung Ho) 대한기계학회 2013 대한기계학회 춘추학술대회 Vol.2013 No.12
For the sustainable development of wind energy, the energy industries have invested in the development of highly efficient wind generator such as an axial flux permanent magnet(AFPM) generator. The AFPM generator, however, has a history of overheating on the stator surface, so that power production decreases significantly. A proper cooling system, therefore, is needed. Although in the case of low capacity generator, a convective-type cooling system has been developed, the size of the air blower must be increased when the generator’s capacity exceeds 2.5MW. In this study, a newly developed conductive-type cooling system was proposed for the 2.5MW AFPM generator installed on an offshore wind turbine. Through electromagnetic-thermal analysis, the characteristics of the heat transfer in the generator were investigated. For the application of the proposed cooling system, the temperatures on the surfaces of stator and permanent magnet were 86 and 68℃, respectively, which could not affect the decrease of the power production efficiency.(KETEP 20134030200320)
[논문 or 기술논문 택일] 대용량 직접구동형 발전기의 열전달 특성에 관한 연구
안대균(Dae Gyun Ahn),노윤철(Yun Cheol Ro),한승호(Seung Ho Han) 대한기계학회 2013 대한기계학회 춘추학술대회 Vol.2013 No.5
Many wind energy industries have invested in the development of a large-scaled direct-drive generator with high efficient power production, such as the axial flux permanent (AFPM) typed generator. The AFPM-typed generator, however, causes overheating on the stator’s surface, so that a proper cooling system is needed. Although a conventional convective-typed cooling system has been developed, there were lots of difficulties for its application to the large-scaled direct-drive generator with capacity exceeding 2.5MW, because the capacity of the devices such as air blower for decrease of high temperature on the stator’s surface should be also grown up. In this study, a new conductive-type cooling system for the 2.5MW AFPM-typed generator was proposed, and the heat transfer characteristics were investigated. In addition, the efficiency of the conductive-type cooling system was estimated in comparison with that of the conventional convective-type cooling system.