비글개에서 신원방우황청심원의 급성 및 아급성독성시험

성하정,권오경,방명주,곽형일,이진영,신대희,박대규,정규혁,윤효인,조명행 성균관대학교 약학연구소 1998 成均藥硏論文集 Vol.10 No.1

Single and 4 weeks oral administration of New wonbangwoohwangchungsimwon (NSCH) which was used l-muscone as substitutive material of musk, to beagle dogs of both sexes were performed to investigate both acute and subacute toxicity. Beagle dogs (3 males and 3 females) in acute experiments were administered orally with sinlge dose of 2,000 ㎎/㎏ and groups of 9 male and 9 female beagle dogs in subacute experiments were given daily different dosage of NSCH, 160 ㎎/㎏/day (low dosage group), 400 ㎎/㎏/day (middle dosage group), 1,000 ㎎/㎏/day (high dosage group) once a day for 4 weeks by oral route according to the Established Regulation of Korea Food and Drug Administration (1996. 4. 16). LD_50 value for beagle dogs was more than 2,000 ㎎/㎏ per oral for both male and females. In animals administered with NSCH, there were neither dead animals nor significant changes of body weights. In addition, no differences were found between control and treated groups in clinical sign, urinalysis, eye examination, hematology, serum chemistry, organ weight and other fingings. No histological lesions were observed in both control and treatment groups. Above data strongly suggset that NSCH in beagle dogs is considered to be safe.

재발성 호흡기 유두종증의 임상양상과 보조 치료의 유용성

성명훈,최병윤,진정욱,성원진,박범정,노종렬,김대우,원태빈,김광현 대한이비인후과학회 2001 대한이비인후과학회지 두경부외과학 Vol.44 No.12

국산 DAF 펌프의 기포특성

안대명,이창한,최윤찬,조석호,안갑환,김성수 대한상하수도학회 2002 상하수도학회지 Vol.16 No.4

The paper introduces a domestic pump designed for dissolved air flotation (DAF). In the paper, the difference in performance between the domestic DAF pump and conventional pressure saturator is explained using the characteristics of air bubble released. The size distribution of air bubbles released from DAF pump is measured by image analyzer and particle size analyzer. And the mean diameter of bubbles is 43μm. The results are similar to that of pressure saturator. The air volume concentration in the water of domestic DAF pump and pressure saturator are 40 and 70% of theoretical amounts. In DAF system used domestic DAF pump, the removal efficiency of turbidity is 70%.

배추 內婚系와 交配後代에서 自家不和合 遺傳因子의 活力變異에 대한 硏究

趙成大,閔炳訓,李政明 경희대학교 부설 식량자원개발연구소 1993 硏究論文集 Vol.14 No.-

배추의 自家不和合 因子組成이 다른 全球(S_(5)S_(5)) 및 野崎 2號(S_(6)S_(6))계통과 이들간의 交配에서 얻은 F₁식물(S_(5)S_(6)), 그리고 F₁을 自殖하여 얻은 F₂植物體群을 상호교배하여 自家不和合 遺傳因子에 대한 優劣關係의 遺傳과 發現樣相을 연구 하였다. F₁ 및 F₂식물체에서의 자가불화합 支配 對立因子間의 관계는 花粉에서 安定的이었으나 株頭에서의 환경적 요인등에 의해 다양하게 변화하는 것이 확인 되었다. F₁식물의 自家不和合 程度나 S-因子間의 優劣關係는 이를 自殖한 F₂식물에서 동일하게 나타나지 않았다. A series of experiments were conducted to investigate the dominance-recessive relationship of S-genes and degrees of self incompatibility in 2 inbred lines of known S-allele and their F₁and F₂ plants by full diallele crosses. The dominance-recssive relationship between S-genes in pollen was stable in F₁and F₂ plants throughout the experiment. In stigma, however, the relationship apparently changed or reversed in F₁or F₂ plants and also possibly by environmental and genetic factors. F₂ plants did not exhibit the same dominance-recessive relationship as in the F₁plants, especially in stigma.

Edwardsiella tarda에 대한 계란난황항체의 분리와 정제

김영대,오명주,정태성,정성주 한국어병학회 2004 한국어병학회지 Vol.17 No.1

Edwardsiella tarda로 면역한 닭은 난황항체(IgY) 정제 방법을 비교하였다. Anti-E.tarda IgY의 정제는 PEG법, chloroform-PEG법, ammonium sulfate법과 정제 kit를 사용한 4가지 다른 방법으로 실시하였다. 정제된 I&Y는 64kDa의 heavy chain과 27 kDa의 light chain을 나타내었다. E.tarda로 면역된 IgY는 면역되지 않은 대조 IgY보다 높은 ELISA가와 응집항체가를 나타내었으며, 정제된 IgY는 western blotting에서 anti-E.tarda 토끼혈청과 유사한 E.rarda 단백질을 인식하였다. PEG법과 ammonium sulfate법에 의해 정제된 IgY는 응집항체가가 1:512, chloroform-PEG법과 정제 kit에 의해 정제된 IgY를 정제하기 위한 가장 빠른 방법이었다. 이 연구의 결과로 PEG법이 IgY의 생물학적 활성을 유지함과 더불어 신속하고 효과적인 정제방법임을 알 수 있었다. The present study compared purification methods of hen egg yolk immunoglobulin (IgY) from the hen immunized with Edwardsiella tarda. The purification of anti-E. tarda IgY was performed by four different methods, polyethylene glycol (PEG), chloroform polyethylene glycol (Chloroform-PEG), ammonium sulfate and purification kit. Purified IgY had heavy chain of 64 kDa and light chain of 27 kDa size. IgY purified from the hen immunized with E. tarda showed higher ELISA values and agglutination titers than those with IgY purified from the non-immunized hen as a negative control. In addition, purified IgY recognized similar E. tarda proteins to those with anti-E. tarda rabbit serum by western blotting. Purified IgY had an agglutination titer of 1 : 5 12 by PEG method and ammonium sulfate method, and 1 : 128 by chloroform-PEG method and purification kit. Moreover, PEG method was the most rapid method among the four different IgY purification methods. These results indicate that PEG method is effective purification method maintaining biological activity of the IgY.

대스팬 지붕구조물의 바람에 의한 동적거동을 고려한 풍하중 평가에 관한 연구

김대영,김지영,김한영,이명호,김상대 대한건축학회 2004 대한건축학회 학술발표대회 논문집 - 계획계/구조계 Vol.24 No.1(구조계)

Generally, wind loads for large span structures are evaluated with surface pressure data acquired in wind tunnel test. Unlike cladding wind loads, the structural wind loads could be over-estimated with only peak pressures of each surface pressure point because the peak pressures on a surface are not well correlated at a given time. Also, the load shapes of wind loads, and the load effects and dynamic behaviors of a structure should be considered in the wind load assessment. In this study, several wind load evaluation methods were applied to Gwangmyeong Velodrome dome and the results were compared to analyze the properties of each method for the suggestion of a proper evaluation procedure.

만성 심부전환자의 혈액에서 측정한 Tumor necrosis factor-α 및 Interleukin-6의 임상적 의의

김명구,김성구,박상호,한대희,강진환,변정득,심규혁,최병조,방덕원,온영근,현민수,권영주 순천향의학연구소 2001 Journal of Soonchunhyang Medical Science Vol.7 No.2

Background and objectives: Many conditions are responsible for the pathophysiology and progressive mechanisms of congestive heart failure. More recently, it has also become evidence that another class of biologycally activated molecules generically reffered to as cytokine these are also over expressed in congestive heart failure. Therefore, the purpose of this study was to measure concentrations of tumor necrosis factor-α(TNF-α), interleukin-6(IL-6) in mild to severe symptoms of heart failure and compare their values with those found in normal control and analysed correlation relationship between cytokine level, clinical findings and hemodynamic indicies. Subjects and Methodology: Levels of TNF-α and IL-6 were measured on pulmonary artery during cardiac catheterization in heart failure patients(n=32) and normal subjects(n=8) as well as physical examination and echocardiogram. Cytokines assay were performed on plasma using commercially available ELISA(Enazyme-Linked Immunosorbent Assay) kits. Results: Although the levels of TNF-α and IL-6 tend to increase in congestive heart failure group, the cytokines level was not made significantly statistical difference between congestive heart failure group and controls. When analyzing the correlation between the levels of PCWP(pulmonary capillary wedge pressure) and cytokines(TNF-α, IL-6), respectively, there were statistically significant correlation coefficient 0.32,(p<0.05), 0.39(p<0.01). The cytokine IL-6 and pressure of pulmonary artery were significant correlation.(correlation coefficient 0.36, p<0.02) More significantly, there was correlated with TNF-α and IL-6.(correlation coefficient 0.57, p<0.001) Conclusions: There was tended toward high concentration of TNF-α & IL-6 in congestive heart failure and significant difference for PCWP between TNF-α & IL-6, thus may be correlated with development and progression in congestive heart failure.

다운홀 시험결과의 신뢰도 향상을 위한 자료해석법

조명진,장대우,조성호 中央大學校 建設環境硏究所 2002 環境科學硏究 Vol.13 No.1

파의 부정확한 도달시점에 의해 전단파의 속도가 결정된다면, 동적 탄성계수 또한 부정확하게 된다. 반면, 전단파의 도달시점을 정확히 결정하여 전단파 속도 측정의 정확성을 향상할 수 있다면, 상대적으로 신뢰성이 작지만, 경제적이고 효율적인 다운홀 실험의 적용성을 극대화시킬 수 있다. 따라서, 본 연구에서는 다운홀 시험에서 전단파 속도를 가장 정확하게 결정하는 방법을 결정하기 위해서 현재 적용가능한 도해법, 최대곡률법, 상호교차법, 상호상관법, 위상속도법 등을 비교검토하였다. 또한, 각 깊이에서의 전단파 전파시간으로부터 전단파 속도 주상도를 결정하기 위한 다운홀 해석법인 직접법, 수정 간접법, 역산 해석법 등과 신뢰도가 높은 SASW실험결과를 비교하였다. 그 결과 전달파 전파시간의 결정은 최대곡률법과 교차상관법이 적합한 것으로 판단되었고, 다운홀 해석 법으로는 Snell 법칙을 이용하는 역산해석 법이 신뢰도가 높은 것으로 판단되었다. In case of visual measurement of velocity, there is a potential error for the arrival point of shear wave. Consequently, the error causes incorrect results in the evaluation of dynamic modulus. The disadvantage of downhole test can be improved, if the velocity of shear wave is exactly chosen by arrival point of the wave. Thus, in this study, visual method, cross-over method, cross-correlation method and phase of cross spectrum method were used to find the most reliable procedure for the determination of the S-wave velocity Also, to provide the reliable interpretation procedure to evaluate the S-wave velocity profile from travel times at depths, the results of the direct method, the modified interval method, arid the inversion method were compared with the results of SASW measurments. As a result, the modified interval method and the inversion method based on Snell's method turned out to be the reliable procedures to determine the S-wave velocity profile from the travel times of the S-wave at depths.

오징어젓 숙성 중 N-nitrosamine 생성에 관한 연구

김성수,오창경,오명철,송대진,김수현 濟州大學校 産業技術硏究所 1996 산업기술연구소논문집 Vol.7 No.1

Possibility of formation of carcinogenic N-nitrosamines and changes in their precursors during the fermentation of high salt-fermented squid, Sepiell maindroni, group with 20% of salt and low salt-.fermented squid group with 10% of salt, 6% of ethanol, 6% of sorbitol and 0.5% of lactic acid were investigated. pH during the fermentation of squid changed in the ranges of 5.79 ~6.07 and 6.05~6.21 in low and high salt group, respectively. The contents of nitrite- and nitrate-N during the fermentation were in the ranges 0.75~1.04 and 1.22~1.42mg/kg, respectively. Trimethylamine(TMA) was continuously .increased during the fermentation and showed 20.1 and 14.9mg% in high and low salt group after 140 days of the fermentation, respectively. while trimethylamine oxide(TMA0) was gradually increased from the beginning until the middle period of fermentation and then was again decreased. Dimethylamine(DMA) was continuously increased during the fermentation and showed 19.7 and 13.3mg/kg in high and low salt group after 140 days of the fermentation, respectively. N-nitrosamines in salt-fermented squid was detected only N-nitrosodimethylamine(NDMA), which its amount was trace in low salt group and 3.8~g/kg in high salt group on 40 days of the fermentation and then continuously increased in the ranges of 57.5~74.7μg/kg in both groups during the fermentation.

朝鮮時代 地方都市의 空間構成原理에 關한 硏究(2) : 大邱府城內의 官廳施設을 중심으로

예명해,신상화,이용대 대구대학교 과학기술연구소 1998 科學技術硏究 Vol.5 No.3