하악측두관절에서 퇴행성 관절염의 실험모델 개발

신상훈,송동석,김철훈,황대석,김용덕,김욱규 부산대학교 병원 암연구소 2007 부산대병원학술지 Vol.- No.21

Purpose : The purpose of this study is to investigate temporomandibular joint arthritis that was induced by intra-articular injection of collagenase in rabbits. Material and Methods: In this study, 12 rabbis was used. We injected collagenase on temporomandibular joint of rabbit and observed for six weeks. H-E stain was done. Results : Four weeks later from 2.0 mg collagenase injection, we observe histologic appearance like arthritis. Conclusion : In our study using injection of collagenase in temporomandibular joint of rabbit we produce arthritis on temporomandibular joint of rabbit.

대뇌 기저핵 신경세포 일차배양에서 망간에 의해 유도된 Appoptosis : 형태학적인 소견

신동훈,김상표,정용욱,배재훈,송대규,백원기 大韓産業醫學會 2000 대한직업환경의학회지 Vol.12 No.1

목적 : 본 실험은 대뇌기저핵의 신경세포를 배양하여 망간(MnCl₂)을 투여한 후 망간독성에 의한 신경세포의 apoptosis를 형태학적인 소견으로 관찰하였다. 방법 : 배양된 신경세포에 0.01에서 10μM Mnc12를 48시간동안 처리한 후 TUNEL (TdT-mediated dUTP Nick End Labelling)법 및 투과전자현미경학적으로 관찰하였다. 결과 : TUNEL방법을 이용하여 관찰한 결과 TUNEL반응에 갈색으로 양성반응을 나타내는apoptotic 세포의 수가 대조군에 비해 MnCl₂를 투여한 군에서 유의하게 높게 나타났으며(P〈0.05), 투과전자현미경학적 소견상 대조군의 신경세포들은 핵인(nucleolus)이 두드러지게 특징적으로 보이면서 핵막과 세포질내 소기관들이 잘 보존되어 있으며, 세포질내망(ER)과 사립체(mitochondria)를 특히 많이 가지고 있었다. Mnc12를 48시간 동안 처리한 군에서 이질염색질(heterochromatin)이 핵막으로 이동하면서 응집되어 있었으며, 핵내 불규칙한 형태의 염색질이 나타나 분절이 진행되는 소견을 보였고, Apoptosis의 가장 특징적인 초기 소견인 막으로 둘러싸인 반달모양의 핵내염색질의 분절편(fragment-ed chromatin)과 주위의 상대적으로 정상적인 소기관으로 구성된 apoptotic body를 관찰할 수 있었다. 결론 : 신경세포에서 망간에 의해 apoptosis가 유도됨을 형태학적인 방법으로 확인할 수 있었으며 망간에 의한 세포사망양상에 apoptosis가 하나의 기전이 될 수 있을 것이다. Objectives : Manganese is cytotoxic to the central nervous system including basal gan-glia. Its toxic mechanism is related to oxidative stress, mediated by toxic free radicals but is specultives. In the present study , we have investigated to manifest apoptosis in man-ganese-induced cytotoxicity in primary neuronal cell culture of rat basal ganglia. Methods : To detect apoptotic neuronal cells were stained by the terminal deoxynu-cleotide (TdT) -mediated dUTP nick end-labelling (TUNEL) method and apoptotic changesin nuclei of neurons were observed by electron microscopy. Results : We showed that TUNEL immunostain showed brownish signal in the nuclei of apoptotic cells and the proportions of apoptotic cells in Manganese treatment groups were more higher than controls. On transmission electron microscopy, there were chromatine condensation with margination toward nuclear membrane and condensation of cyto-plasm in the treated with luM MnCl²for 48 hours in a basal ganglia neurons. Apoptotic bodies were found and consisted of semilunar-like condensed nuclei with relatively intact cytoplasmic organelles. Conclusions : Apoptosis appears to be one mechanism in the manganese-induced net-ronal cell death. Manganese intoxication is a convenient model for apoptosis study.

하악과두에 발생하는 골연골종의 진단학적 방법

신상훈,차승만,황대석,김용덕,김욱규 부산대학교 병원 암연구소 2006 부산대병원학술지 Vol.- No.20

Pulpose : Osteeochondroma is a common benign tumor of the axial skeleton, especially the distal metaphysis of the femur and the proximal metaphysis of the tibia, however, is\ occured rarely on the facial skeleton. Tumors of the mandibular condyle are various and rare. so common interest is a little and its clinical symptoms are similar to the others. therefore, early diagnosis is very difficult. In this study, we will present our cases and review the literatures. Patients and Methods: We studied 17 patients(8 males, 9 females) who were diagnosed a osteochondroma of the mandibular condyle in the department of oral and maxillofacial surgery, Pusan National University Hospital. We reviewed the medical records. Results : Patients' mean age was 30.05 years. The sexual distribution is almost same. Twelve left condyles and five right condyles were involeved. The main symptoms was noise and facial asymmetry. Conclusion : Osteeochondroma is a rare benign tumor of the mandibular condyle. The differential diagnosis is difficult. However, using CT and MRI, we diagnosed early the osteochondroma and treated properly. Thus, we presented the cases of the osteochondroma with the literature reviews.

흉·요추부 골절환자의 수술적 치료 결과

신규철,강대술 건국대학교 의과학연구소 1998 건국의과학학술지 Vol.8 No.-

There have been progressive increase in use of internal fixator for the treatment of thoracolumbar spinal fracture that offers several advantages such as more rigid fixation and stabilization of the fractured spine, earlier mobilization and rehabilitation, prevention of the further deformity, shortening of the hospitalization. We analyzed the clinical outcomes of 28 cases of thoracolumbar spinal fracture patients who had an operation from January 1994 to January 1997. The results obtained are as follows; 1. Of 28 cases, 17 cases were male and 11 cases were females. Average age was 42 years. 2. The most common site of injuries was L1 (11 cases). 3. The surgical indications are 1) 40% reduction of the height of anterior surface of the vertebra in compression fracture 2) the unstable bursting fracture 3) association with more than 35 degrees kyphotic deformity 4) posterior soft tissue injury in flexion-distraction injury 5) associated with neurologic symptoms. 4. The clinical outcomes as relief of pain, adaptation for the previous job, postoperative complications are good. 5. The proper surgical indication is to be considered as the most important factor in surgical treatment of the thoracolumbar spinal fracture.

응급실을 내원한 구강악안면외과 안면골 골절 환자의 변화에 대한 비교 연구

신상훈,성화식,강영훈,황대석,김용덕,김욱규,김종렬,정인교 부산대학교 병원 암연구소 2008 부산대병원학술지 Vol.- No.24

Although there have been many clinical studies about the facial trauma in the recent as well as in the past, severity and frequency of facial trauma are reported multifarious. It seemed to be because of variety of social, cultural, and environmental factors, and the view point of investigators. In this study, we investigated about the patients visiting emergency room during recent 4 years(2004-2007), and compared with retrospective study during former 10 years(1992-1996, 1999-2003) in Dept. OMS Pusan National University Hospital. We assessed the cause, type, demographic, etc, and analyzed alterations of facial bone fractures during past 3 periods. The results were as follows. The total number of patients was increased. 429 patients treated for maxillofacial fractures between January 1992 and December 1996, 466 patients treated between January 1992 and December 1996 and 764 patients treated between January 2004 and December 2007. The male-to-female ratio were 4.6:1, 3.9:1, and 4.16:1, respectively. Patient in the age groups of 10-19 years and 20-29 years increased in the latter two periods. Assaults and falls are decreased gradually, but slip down s are increased. Traffic accidents are still major cause of facial trauma. The mandibular fractures showed the highest incidence thorought three periods. Symphysis fractures of mandible was increased and condyle fractures of mandible was drecreased in the third period.

악안면 영역에서의 유리 전완피판술 적용례분석

김욱규,송원욱,황대석,김용덕,신상훈,김종렬,정인교 부산대학교 병원 암연구소 2009 부산대병원학술지 Vol.- No.25

구강내 결손부에 적용된 연조직 재건술식들에 대한 임상적 고찰

김욱규,이승환,황대석,김용덕,신상훈,김종렬,정인교 부산대학교 병원 암연구소 2007 부산대병원학술지 Vol.- No.21

To evaluate criteria, indications, and prognosis of the various reconstructive methods on the patients with intraoral soft tissue defect who had been treated at Dept. of Oral and Maxillofacial Surgery, Pusan National University Hospital from 2003 to 2005, we have reviewed the clinical data of the patients and analysed. The results were as follows: 1. Tongue flaps have been mainly applied on anterior portion of palate and maxilla. The survival rate was high percent, but the cooperation of patient was inevitable for the success. 2. Palatal mucosa rotational flaps were available on relative large defect on palate, oroantral fistula site. The side effect was a scaring band from secondary healing on denuded donor palate site. Sometimes the band came to be a hinderance to swallowing, phonation. 3. The application of free skin graft on entire maxilla site , palate was possible. The skin from thigh, abdomen could be applicable on fixed or movable tissues. The advantages of abdominal skin were easy harvest and direct donor site closure. 4. Forearm free flap was a workhorse flap for everywhere in intraoral defects. We had used the flap on cheek, floor of mouth, tongue without any significant complications. But the application of the flap was required for long operation time, which was disadvantageous to the old, weak patients. 5. Cervical platysmal flap could be easily applicable for buccal cheek, floor of mouth after excision of the cancer lesion. the design of the flap could be made simultaneously on neck dissection, but the danger of cancer remnants on the flap always might be remained. 6. Buccal fat pad pedicled flap must have been a primary flap for repair of oroantral fistula especially on posterior maxilla. The flap survival will be expected if the considerations for above reconstructivε methods on site, size, condition of defects primarily could be made.

횡문근융해증과 급성 신부전 및 범발성 혈관내 응고장애를 동반한 열사병 1예

최대은,박수진,권오경,이한규,이영모,이상주,나기량,이강욱,신영태 충남대학교 의학연구소 2003 충남의대잡지 Vol.30 No.2

Heat stroke is a life-threatening illness characterized by an elevated core body temperature that rises above 40% and central nervous system dysfunctions that results in delirium, convulsions, or coma. Heat stroke is subdivided into two forms, classic and exertional. Since strenuous exercise is one of the major exacerbating and precipitating factors, the incidence of exertional heat stroke is high among young adult, especially military personnel undergoing military training. Exertional heat stroke commonly causes rhabdomyolysis, lactic acidosis, acute renal failure, shock and pulmonary edema. We report a case of heat stroke with rhabdomyolysis, acute renal failure, disseminated intravascular coagulopathy(DIC) after strenuous exercise. A 18 year old man was admitted to the hospital because of loss of consciousness. He was a wrestler and he had tried to lose 6kg in weight by jogging and strenuous exercise on that summer day with hot temperature. The development of this illness was favoured by much clothes inappropriate for that environmental condition with a purpose to enhance sweating, and by restricted intake of water and food. On admission, the blood pressure was 80/50mmHg, and body temperature was 39.4℃ with stuporous mentality. Laboratory finding were as follows : in complete blood cell count, hemoglobin was 16.7g/dL and platelet count was 158,000/ mm³. In blood chemistry, AST 1061U/L, ALT 201U/L, BUN 30mg/dL, Cr 2.2mg/dL, LDH 941IU/L, CK 696IU/L were resulted. In arterial blood gas analysis, pH 7.39, pCO₂ 30mmHg pO₂ 86mmHg, HCO₃ 18.4mEq/L, O₂ saturation 96.7% were resulted. In urinalysis, specific gravity 1.025, protein 3+, erythrocyte 2+ were resulted. In brain imaging study, brain CT finding was normal. After appropriate treatments with rapid cooling, hydration and supportive care, he improved with alert mental status. On 2nd hospital day, in laboratory result, thrombocytopenia and coagulopathy were appeared and renal insufficiency was aggravated. So he was treated with platelet concentrate and fresh frosen plasma for DIC, and enough hydration for appropriate urine volume preservation. After all, On 22nd hospital day, he fully recovered and discharged. Nowadays, with followed for some duration, he is wellbeing with no complications.

위발성 위장관 악성림프종 환자에서 항암치료의 효과

김찬규,신영록,김현정,배상병,이남수,이규택,박성규,원종호,홍대식,박희숙 순천향대학교 2006 Journal of Soonchunhyang Medical Science Vol.12 No.1

Purpose: The gastrointestinal (GI) tract is the most common site of extranodal non-Hodgkin's lymphoma (NHL), which is increasing in incidence, but there is no established optimal treatment modality. Thus, this study was investigated the clinicohistologic feature, the therapeutic modalities, and the prognosis for GI-NHL, as well as the factors affecting it. Methods: We retrospectively analyzed 45 patients who had been diagnosed as having GI-NHL and had been followed up from July 1994 to February 2005 at Soonchunhyang University Hospital. The patients were divided into groups according to the site of origin and to various other features, and the survivals of the various groups were compared. The modified Ann Arbor system and WHO classification were adopted for staging and histopathologic classification, respectively. Results: GI-NHL of the stomach, small bowel, ileocecal region, and colon occurred in 28 patients (62.2%), 5 patients (11.1%), 3 patients (6.7%), and 8 patients (17.8%), respectively, In one patient, the entire gastrointestinal tract was diffusely involved. The median age of patients was b5 years (25~78 years), and male-to-female ratio was 1:1.1. Fourteen patients were in stage Ⅰ, 24 in stage Ⅱ, 4 in stage Ⅲ, and 3 in stage Ⅳ. Surgical resection was performed in 19 patients, and combination chemotherapy was performed in 43 patients. Surgical resection only was performed in 4 patients, Chemotherapy only was performed in 26 patients. The expected overall 5 year survival of 45 patients was 39.6%, and there was a significant survival difference between the stages, but between sites of origin (p=0.842). The most important factors influencing the survival was the stage and other factors were not significant. Conclusion: The stomach was the most common site of GI-NHL. Most GI-NHL were localized Stage was the most important prognostic factor. However, Prospective randomized studies are needed to approve the therapeutic modality.

포도호랑하늘소의 生態 및 加害特性에 關한 硏究

金奎眞,李在休,朴鍾大,申炫雨 全南大學校 農漁村開發硏究所 1988 農業科學技術硏究 Vol.23 No.-

포도호랑하늘소의 生態 및 加害特性을 究明코자 1983~1985. 3個年에 걸쳐 全南 山間地이며 울타리式 포도栽培團地인 谷城과 平野地이며 天井式 포도 栽培團地인 羅州에서 遽行하였다. 1. 포도호랑하늘소의 形態的인 特徵을 보면 成蟲은 암컷의 크기가 12.6mm, 숫컷 11.7mm, 용`14.8mm, 卵은 長徑 1.12mm, 短俓 0.44mm 1마리 雄成蟲 産卵數는 15~53個 抱卵數는 29~108個였다. 한편 各態別 期間은 卵基間 7~12日 幼蟲期 268~303日,용期 12~15일, 成蟲 24~41日程度였다. 2. 本蟲은 年 1回 發生하여 成蟲의 發生其間은 山間地인 谷城에서는 7月中間~9月中旬으로 그 最盛期는 8月 中旬頃이었으며, 平野地인 羅州에서는 8月中旬~9月中旬으로 그 最盛期는 9月上旬頃이었다. 3. 被害莖은 3年以上의 가지로서 直徑이 9014mm에서 많았다. 4. 品種에 다른 被害率은 거봉 34.7% Muscat Bailey A29.8% Dela ware 27.3%, Campbell Early 22.9%였다. 5. 越冬態의 蟲齡別 越冬率은 1齡 8.4% 2齡 75.4%, 3齡 12.7% 4齡 3.5%였다. 6. 포도원의 剪定後 剪定地를 放置한 경우에는 45.7% 除去한 경우는 7.6%의 被害率을 보였다. 7. 포도호랑하늘소의 幼蟲과 용에寄生하는 天敵으로서 주머니응애(Pediculoides ventrico년)寄生蟲(Odontobracon bicolar)이 調査되었으며 特히 주머니용애는 寄生率이 높았다. This study was carried out to investigate Bionomics and Damaged aspect on the Grape tiger longicorn Xylotrechus pyrrhoderus at Goksung, mountain area and Naju, field area from 1983 to1985. In size of each stage, female adult was 12.6mm male adult 11.7mm, pupa 14.8mm, egg length 1.22mm, width 0.44mm. Periods of egg was 7 to 12 days, larva 268 to 303 days, pupa 12 to 15 days, adult 24 to 41. days No. of eggs in ovary and laid was 29 to 108 and 15 to 53, respectively but was affected by environmental condition. Grape tiger longicorn have one generation under natural condition. In mountain area, adults emerged from mid-August to mid-September and peak was mid--August.In field area, adults emerged from mid-August to mid-September and peak was early-September. Rate of injury in grape vine stem was great above 3 years branch and damages by Grape varactics were 34.7% in Kyoho, 29.8% in Muscat Bailey A, 27.3% in Dela Ware. 22.9% in campbell Early, respectively. Overwintering larva was great in 2nd instar of 75.4%. Natural enemies of Grape tiger longicorn was investigated in the larva and pupa, Pediculodes ventricosus(Acarina) Odontabracor bicolar(Hymennoptera)