Comparative Efficacy of Different Soy Protein Sources on Growth Performance, Nutrient Digestibility and Intestinal Morphology in Weaned Pigs

Yang, Y.X.,Kim, Y.G.,Lohakare, J.D.,Yun, J.H.,Lee, J.K.,Kwon, M.S.,Park, J.I.,Choi, J.Y.,Chae, B.J. Asian Australasian Association of Animal Productio 2007 Animal Bioscience Vol.20 No.5

To elucidate the efficacy of different soy protein sources on piglet's performance, a total of 280 weaned piglets ($Duroc{\times}Yorkshire{\times}Landrace$, $23{\pm}3$ d of age, $5.86{\pm}0.45$ kg initial BW) were allotted to 5 treatment diets comprising soybean meal (SBM), soy protein concentrate (SPC), Hamlet protein (HP300), fungal (Aspergillus oryzae) fermented soy protein (FSP-A), and fungal plus bacterial (A. oryzae+Bacillus subtilis) fermented soy protein (FSP-B), respectively. Experimental diets for feeding trial were formulated to contain each soy protein sources at 8% level to corn-whey powder basal diet. There were 14 pigs per pen and 4 pens per treatment. Experimental diets were fed from 0 to 14 d after weaning and then a common commercial diet was fed from 15 to 35 d. Also for ileal digestibility studies, 18 pigs were assigned to 6 dietary treatments as N-free, SBM, SPC, HP300, FSP-A and FSP-B with T-canulation at distal ileum for 6 days. At $14^{th}$ d of experimental feeding, the ADG was significantly higher (p<0.05) in SPC fed diet as compared with others. Similar trend was noticed during the 15-35 d and overall study (0-35 d). All the processed soy protein sources tested in this experiment improved (p<0.05) growth than SBM during overall study. The nutrient digestibility of GE, DM, CP and Ca showed lower (p<0.05) values in SBM and FSP-A fed groups than SPC and FSP-B treatments. The apparent ileal digestibility of TEAA, non-TEAA and TAA showed lower (p<0.05) in SBM treatments compared with other soy protein sources. The true ileal digestibility of TEAA, non-TEAA and TAA were lower (p<0.05) in SBM fed group than SPC and HP300 treatments, and lower than FSP treatments though they didn't achieve significant difference (p>0.05). Villous height and crypt depth was not affected by dietary treatments. In conclusion, the growth and digestibility of nutrients in weaned pigs fed SPC was superior to others. Also FSP-A and FSP-B showed improved performance than those fed SBM.

Protein Profile in Corpus Luteum during Pregnancy in Korean Native Cows

Chung, H.J.,Kim, K.W.,Han, D.W.,Lee, H.C.,Yang, B.C.,Chung, H.K.,Shim, M.R.,Choi, M.S.,Jo, E.B.,Jo, Y.M.,Oh, M.Y.,Jo, S.J.,Hong, S.K.,Park, J.K.,Chang, W.K. Asian Australasian Association of Animal Productio 2012 Animal Bioscience Vol.25 No.11

Steroidogenesis requires coordination of the anabolic and catabolic pathways of lipid metabolism, but the profile of proteins associated with progesterone synthesis in cyclic and pregnant corpus luteum (CL) is not well-known in cattle. In Experiment 1, plasma progesterone level was monitored in cyclic cows (n = 5) and pregnant cows (n = 6; until d-90). A significant decline in the plasma progesterone level occurred at d-19 of cyclic cows. Progesterone level in abbatoir-derived luteal tissues was also determined at d 1 to 5, 6 to 13 and 14 to 20 of cyclic cows, and d-60 and -90 of pregnant cows (n = 5 each). Progesterone level in d-60 CL was not different from those in d 6 to 13 CL and d-90 CL, although the difference between d 6 to 13 and d-90 was significant. In Experiment 2, protein expression pattern in CL at d-90 (n = 4) was compared with that in CL of cyclic cows at d 6 to 13 (n = 5). Significant changes in the level of protein expression were detected in 32 protein spots by two-dimensional polyacrylamide gel electrophoresis (2-DE), and 23 of them were identified by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS). Six proteins were found only in pregnant CL, while the other 17 proteins were found only in cyclic CL. Among the above 6 proteins, vimentin which is involved in the regulation of post-implantation development was included. Thus, the protein expression pattern in CL was disorientated from cyclic luteal phase to mid pregnancy, and alterations in specific CL protein expression may contribute to the maintenance of pregnancy in Korean native cows.

착유우의 사료섭취량과 음수량이 산유량과 분뇨 배설량에 미치는 영향

최동윤,곽정훈,박치호,정광화,김재환,유용희,양창범,최홍림,안희권,Choi, D.Y.,Kwag, J.H.,Park, C.H.,Jeong, K.H.,Kim, J.H.,Yoo, Y.H.,Yang, C.B.,Choi, H.L.,Ahn, H.K. 한국축산환경학회 2007 축산시설환경학회지 Vol.13 No.1

본 실험은 우리나라에서 사육되고 있는 홀스타인 젖소 착유우의 사료 섭취량과 음수량이 산유량과 분뇨 배설량에 미치는 영향을 구명하기 위하여 수행하였다. 실험은 착유우 10두를 공시하여 수원의 축산연구소 낙농우사에서 봄, 여름, 가을, 겨울 등 4계절에 걸처 실시하였으며 그 결과는 다음과 같다. 1. 시험에 공시된 착유우의 건물섭취량은 평균 19.5kg으로 봄과 가을이 각각 23.9, 22.1kg으로 높았고, 여름과 겨울이 각각 17.0, 15.3 kg으로 낮은 섭취량을 보였다. 2. 착유우의 음수량은 평균 $77.2\ell$로, 여름이 $85.5\ell$로 가장 많았고 겨울이 $62.2\ell$로 가장 낮았으나 계절별 유의차는 보이지 않았다. 3. 착유우의 산유량은 평균 26.0kg으로 봄, 여름, 가을 및 겨울이 각각 30.8, 24.0, 25.4, 23.7kg으로 봄의 산유량이 가장 높았다(p<0.05). 4. 착유우의 분뇨 배설량을 계절별로 조사한 결과, 봄 64.4kg, 여름 63.5kg, 가을 60.4kg, 겨울 51.0kg으로 계절 간에 차이를 보였으며(p<0.05), 전체 평균 분뇨 배설량은 59.8kg이었다. 5. 착유우의 사료섭취량, 음수량, 산유량 및 분뇨 배설량과의 상관관계를 분석한 결과, 산유량과 음수량$(R^2=0.7742)$, 산유량과 사료 섭취량$(R^2=0.7459)$, 음수량과 뇨 배설량 $(R^2=0.7422)$, 사료 섭취량과 분 배설량$(R^2=0.6044)$, 산유량과 분 배설량$(R^2=0.692)$의 상관관계가 비교적 높았으나, 음수량과 분 배설량$(R^2=0.2950)$, 사료 섭취량과 뇨 배설량$(R^2=0.1985)$, 산유량과 뇨 배설량$(R^2=0.2335)$의 상관관계는 낮게 나타났다. 6. 따라서 산유량과 음수량, 산유량과 사료 섭취량의 상관관계식은 $Y=0.1919X_1+11.181(R^2=0.7742),\;Y=0.8568X_2+9.3067(R^2=0.7459)$(Y=milk yield $X_1=water$ consumption, $X_2=feed$ intake)로 추정할 수 있다. This experiment was conducted to determine the effect of feed intake and water consumption on milk yield and manure production in milking cows. The average feed intake(dry matter) of milking cows were 19.5kg/hd/d. Spring(23.9kg) and fall(22.1kg) feed intake rates when higher than in the summer(17.0kg) and winter(15.3kg/hd/d). The average water consumption of milking cows were $77.2\ell/hd/d$. Summer showed the highest value$(85.5\ell/hd/d)$ and winter showed the lowest value$(62.2\ell/hd/d)$. The average milk yield during spring, summer, fall, and winter was 30.8, 24.0, 25.4, and 23.7kg/hd/d, respectively. Milk yield during spring was found to be statiscally greater than for the other seasons. Manure production of milking cows during spring, summer, fall, and winter was 64.4, 63.5, 60.4, and 51.0kg/hd/d, respectively. Consequently, a relatively high correlation between milk yield and water consumption$(R^2=0.7742)$, milk yield and feed intake$(R^2=0.7459)$, water, consumption and urine production$(R^2=0.7422)$, feed intake and feces production$(R^2=0.6044)$, and milk yield and feces production$(R^2=0.6920)$ were observed in milking cows. The other hand, correlation between water consumption and feces production$(R^2=0.2950)$, feed intake and urine production $(R^2=0.1985)$, and milk yield and urine production$(R^2=0.2335)$ were found to be relatively low. Therefore, correlation equation between milk yield and feed intake, milk yield and water consumption can be estimated from : $Y=0.1919X_1+11.181(R^2=0.7742),\;Y=0.8568X_2+9.3067(R^2=0.7459)$(Y=milk yield $X_1=water$ consumption, $X_2=feed$ intake).

Forebrain-specific ablation of phospholipase Cγ1 causes manic-like behavior

Yang, Y R,Jung, J H,Kim, S-J,Hamada, K,Suzuki, A,Kim, H J,Lee, J H,Kwon, O-B,Lee, Y K,Kim, J,Kim, E-K,Jang, H-J,Kang, D-S,Choi, J-S,Lee, C J,Marshall, J,Koh, H-Y,Kim, C-J,Seok, H,Kim, S H,Choi, J H,Ch Macmillan Publishers Limited, part of Springer Nat 2017 Molecular psychiatry Vol.22 No.10

<P>Manic episodes are one of the major diagnostic symptoms in a spectrum of neuropsychiatric disorders that include schizophrenia, obsessive-compulsive disorder and bipolar disorder (BD). Despite a possible association between BD and the gene encoding phospholipase C gamma 1 (PLCG1), its etiological basis remains unclear. Here, we report that mice lacking phospholipase C gamma 1 (PLC gamma 1) in the forebrain (Plcg1(f/f); CaMKII) exhibit hyperactivity, decreased anxiety-like behavior, reduced depressive-related behavior, hyperhedonia, hyperphagia, impaired learning and memory and exaggerated startle responses. Inhibitory transmission in hippocampal pyramidal neurons and striatal dopamine receptor D1-expressing neurons of Plcg1-deficient mice was significantly reduced. The decrease in inhibitory transmission is likely due to a reduced number of gamma-aminobutyric acid (GABA)-ergic boutons, which may result from impaired localization and/or stabilization of postsynaptic CaMKII (Ca2+/calmodulin-dependent protein kinase II) at inhibitory synapses. Moreover, mutant mice display impaired brain-derived neurotrophic factor-tropomyosin receptor kinase B-dependent synaptic plasticity in the hippocampus, which could account for deficits of spatial memory. Lithium and valproate, the drugs presently used to treat mania associated with BD, rescued the hyperactive phenotypes of Plcg1(f/f); CaMKII mice. These findings provide evidence that PLC gamma 1 is critical for synaptic function and plasticity and that the loss of PLC gamma 1 from the forebrain results in manic-like behavior.</P>

Encephalomyocarditis Virus 표면항원의 단일항체 생산세포주의 크론과 이의 면역학 및 생화학적 연구(Ⅱ)

양종대,박종수,이영탁,김화영,김영래,이인수,조영준,박재윤,차종희,윤지원,고광삼 朝鮮大學校 附設 醫學硏究所 1987 The Medical Journal of Chosun University Vol.12 No.1

To see whether there is any differencies in RNA dependent DNA polymerase activities between monoclonal antibody-producing hydridoma cells and non-producing hybridoma cells, Balb/c female mice were immunized with the purified viral surface protein of D-variant of encephalomyocarditis virus and then fused with myeloma cells (NR-1). After cloring, monoclonal antibody-producing hybridoma cell lines were separated from non-producing hydridoma cell lines. RNA-dependent DNA polymerase activities were measured in the supernatant of monoclonal antibody-producing hybridoma clones and non-producing hybr idoma clones, and myeloma cells as control, Monoclonal antibody-producing hybridoma cells showed statistically significant higher activity as compar compare to that of nonproducing hybridoma cells. To find whether RNA-dependent DNA polymerase releasing cells aware secreting or budding C-type virus particles, those cells were examined with electron microscope. The hybridoma cell which secrete large amount of RNA-dependent DNA polymerase shows significant number of extracellular C-type virus particles. In constrast, non-producing hydridoma cells contains a lot of intracellular C-type virus particles. It is concluded that monoclonal antibody-producing hydridoma cells released particles. It is concluded that monoclonal antibody-producing hydridoma cells released significant amount of RNA-dependent DNA polymerase land extracellular C-type virus particles, while non-producing hydridoma cells showed less release of RNA-dependent DNA polymerase and contains intracellular C-type virus particles.

CdS_(1-x)Se_x 광도전 박막의 전기-광학적 특성연구

양동익,신영진,임수영,박성문,최용대 全北大學校 基礎科學硏究所 1994 基礎科學 Vol.16 No.-

본 연구는 CdS_1-XSe_X의 박막을 제작하고 그 전기-광학적인 특성을 조사한 것이다. 전자선 가열증착법을 이용하여 CdS_1-XSe_X을 알루미나 기판위에 1.5× 10 exp (-7) torr의 압력, 4kV의 전압, 2.5mA의 전류 그리고 기판온도를 300℃로 유지하여 증착하였다. 증착된 CdS_1-XSe_X 박막은 X-ray 회절 실험을 통하여 볼 때, 육방정계의 결정구조를 가지며 성장되었다. CdS_1-XSe_X 도전막은 특정분위기에서 550℃, 30분간 열처리함으로써 높은 광전도성을 나타내게 되었다. 또한 Hall 효과, 광전류 스펙트럽, 감도, 최대 허용 전력와 응답시간 등을 조사하였다. We report the crystal growth and the electro-optic characteristics of CdS_1-XSe_X thin films. CdS_1-XSe_X thin films were deposited on the alumina plate by electron beam evaporation technique in pressure of 1.5× 10 exp (-7) torr, voltage of 4kV, current of 2.5 mA and substrate temperature of 300℃. The deposited CdS_1-XSe_X thin films were proved to be a polycrystal with hexagonal structure through X-ray diffraction patterns. CdS_1-XSe_X photoconductive films showed high photoconductivity after annealing at 550℃ for 30 minutes. And the films have been investigated the Hall effect, photocurrent spectra, sensitivity, maximum allwable power dissipation and response time.

D/H VLCC의 피로강도해석과 피로 신뢰성 해석

양박달치(P. D. C. Yang),이주성(J. S. Lee),윤장호(J. H. Yoon),서영석(Y. S. Seo) 대한조선학회 1997 大韓造船學會 論文集 Vol.34 No.2

A study of the electromagnetic characteristics of no-insulation GdBCO racetrack coils under an external magnetic ripple field

Choi, Y H,Yang, D G,Kim, Y G,Kim, S G,Song, J B,Lee, H G IOP 2016 Superconductor science & technology Vol.29 No.4

<P>Here we report the effect of an external magnetic ripple field on the electromagnetic characteristics of GdBCO racetrack coils being operated with a constant DC current. Two types of GdBCO racetrack coils, one wound without turn-to-turn insulation (NI) and the other wound with Kapton tape (INS), were examined under external ripple fields generated by a permanent magnet mounted on a rotor, which was driven by a separate AC motor. The voltage fluctuations and magnetic field variations were measured with respect to the external ripple field intensity (<I>B</I> <SUB>ERF</SUB>), rotating speed, and the operating condition. When the INS and NI coils were exposed to an external ripple field (herein, <I>I</I> <SUB>op</SUB>?=?80 A, <I>B</I> <SUB>ERF</SUB>?=?2 mT, and 5 rpm), a voltage fluctuation occurred because a time-varying magnetic field interacted with an electric circuit creating an electromotive force. The peak-to-peak voltage (<I>V</I> <SUB>pp</SUB>?=?0.29 mV) of the NI coil was ∼1.86 times lower than that (0.54 mV) of the INS coil, because the voltage response of the NI coil lagged behind d<I>B</I>/d<I>t</I> due to the existence of turn-to-turn contact. Furthermore, the <I>V</I> <SUB>pp</SUB> of the INS coil increased with increasing <I>B</I> <SUB>ERF</SUB> and rotating speed, while those of the NI coil were barely affected due to the delay of electromagnetic induction. In excessive current and ripple field conditions (<I>I</I> <SUB>op</SUB>?=?1.125 <I>I</I> <SUB>c</SUB>, <I>B</I> <SUB>ERF</SUB>?=?8 mT, and 50 rpm) the INS coil eventually quenched while the NI coil did not, implying that the electromagnetic stability of the NI coil in excessive time-varying field conditions was superior to that of the INS coil.</P>

강재 절단용 Insert Tip 교환식 D1,840mm급 초대형 원형 톱 개발

양동욱(D. U. Yang),백일현(I. H. Baek),노윤식(Y. S. Ro),김민호(M. H. Kim) 한국정밀공학회 2012 한국정밀공학회 학술발표대회 논문집 Vol.2012 No.5월

소결체의 Nd:YAG 레이저 용접 시 보호가스가 용접강도에 미치는 영향

김용(Y. Kim),양현석(H. S. Yang),박기영(K. Y. Park),이경돈(K. D. Lee) 한국레이저가공학회 2007 한국레이저가공학회 학술대회 논문집 Vol.2007 No.-

  This study includes the effects of shielding gas types and flow rate on Nd:YAG Laser weldability of sintered material. The types of shielding gas were evaluated for He, Ar and N₂. Bending strength, porosity rate, hardness and D/W ratio testing of laser weld are carried out to evaluated the quality for fusion area. As a results, Ar gas was showed the best welding strength even the porosity rate is the most value, and increased the flow rate was also improvement the bending strength. Therefore we could know that bending strength is not only affect the porosity rate but also melting degree.