상피성 난소암에서 항암약물요법(CT 또는 PT)과 ATP-CRA검사의 연관성

이천준,김원규 고신대학교의과대학 2008 고신대학교 의과대학 학술지 Vol.23 No.4

Background: The behavior of cancer can be very varied with different individual responses to chemotherapy. Individualization is crucial to the optimization of chemotherapy. The individualized chemotherapy sensitivity test has been introduced to help in the selection of the appropriate drug for each individual patient but disappointing results achieved with old chemosensitivity tests. The development of the adenosine triphosphate based chemotherapy response assay(ATP-CRA) was designed to overcome the limitations of many in vitro chemotherapy sensitivity tests. The aims of this study were to predict accurately the ATP-CRA and a patient's clinical response to chemotherapy(CT(carboplatin, paclitaxel), PT(cisplatin, paclitaxel)) in epithelial ovarian cancer and to assess the clinical efficacy of the ATP-CRA. Materials and Methods: The study was performed on 34 patients who is diagnosed initially the epithelial ovarian cancer for chemotherapy after operation at Gospel hospital of Kosin university between March 2005 and December 2007. The ATP-CRA was evaluated the chemosensitivities of nine anticancer drugs for epithelial ovarian cancer. To investigate the correlation between ATP-CRA and clinical outcomes in patients with epithelial ovarian cancer, it was compared the clinical responses with the results of CSA retrospectively. Statistical analysis was performed using the Fisher's exact test. Results: The mean chemosensitivity index(CI) tested by the ATP-CRA, were 186.4(carboplatin), 167.9(cisplatin) and 194.1(paclitaxel) respectively. The Cisplatin showed the most effective effect. The sensitivity and specificity of ATP-CRA were 96.4% and 50.0%. The positive and negative response prediction values were 90.0% and 75.0%. The accuracy rate was 88.2%. There was a significant relationship between the results of ATP-CRA and clinical responses(p=0.04). Conclusions: This study shows that ATP-CRA could be used clinically to predict chemoresponse in epithelial ovarian cancer. However, prospective randomized clinical trials in a larger patient cohort are warranted to confirm the clinical correlation of ATP-CRA.

중증 임신성 혈소판감소증 분만 1예

이태화,이천준 고신대학교의과대학 2007 고신대학교 의과대학 학술지 Vol.22 No.2

Gestational thrombocytopenia can be defined either as a specific disorder or as a group of thrombocytopenic disorders that occur in pregnancy. Some physicinas have defined gestational thrombocytopenia as the benign thrombocytopenic disorder that occurs in pregnancy at a relatively high frequency. But severe Gestational thrombocytopenia(platelets<5,000/mm3) is rare. The pathogenesis of thrombocytopenia in the gestation is not fully understood. The primary goal in managing a gestational thrombocytopenia is to deliver a healthy infant without unwarranted risk to the mother's health. We experienced a case of delivery in severe gestational thrombocytopenia so we report this case with a brief review of literature.

만성 무배란에 의한 불임증과 자궁내막암이 동반된 젊은 여성에서의 보존적 치료 경험 1예

최종열,이천준,김원규 고신대학교의과대학 2007 고신대학교 의과대학 학술지 Vol.22 No.2

Endometrial cancers which occur in genetically susceptible women under 40 years of age are related to prolonged unopposed estrogen stimulation of endometrium. Most of these patients complain of menstrual disorder, infertility, hirsutism, obesity, and hypertension, occasionally accompany with polycystic ovarian syndrome or functioning ovarian tumor. Most of these endometrial cancers have a good prognosis, but it has rarely been reported a death, because lesions are histologically well-differentiated and within the limit of endometrium. These young women with well-differentiated endometrial adenocarcinoma who desire a pregnancy can be treated with high-dose progestin, which induces endometrial attenuation, thereafter they can try to induce ovulation for a pregnancy. However, it must be recognized that occasionally recurrent endometrial cancer has been reported in such a consevative treatment. If the endometrium has recurrent, persistent or progressive lesion or follow-up of patient is impossible, the surgery such as total hysterectomy must be performed. We experienced a well-differentiated adenocarcinoma of endometrium in 29 years old infertile woman with chronic anovulation in July 1993. This young woman was treated successfully with high-dose progestin therapy and ovulation induction with clomiphen citrate, finally she succeeded in a pregnancy by in vitro fertilization program 6 years later, in July 1999, and was delivered of a 2,900 gram female by cesarean section. But she was lost follow-up after delivery and not treated for endometrial cancer, and the lesion was reccurred in September 2006, so treated with a total abdominal hysterectomy and bilateral salpingoophorectomy in our hospital ; the pathology was well-differentiated adenocarcinoma and the lesion was limited within the endometrium. Thus we report this case with a brief review of literature.

복강경하 질식 전자궁적출술과 질식 전자궁적출술의 임상적 비교

최종열,이천준,김흥열 고신대학교 의과대학 2007 고신대학교 의과대학 학술지 Vol.22 No.2

Objective : To compare the advantages and disadvantages between laparoscopically assisted vaginal hysterectomy (LAVH) and total vaginal hysterectomy (TVH). Methods : We reviewed the medical records of patients who underwent LAVH and TVH from January 2004 to December 2006. We investigated the difference between LAVH group and TVH group in relation to age, weight, parity, previous abdominal operation, indication for hysterectomy, uterine weight, combined operation, operation time, postoperative hemoglobin change, gas out day, postoperative discharge day, and complications. Results : The most common indication of both group was uterine leiomyoma and/or adenomyosis, especially carcinoma in situ of uterine cervix or cervical cancer Ia1 were more frequent indication in LAVH group than TVH group (p=0.018). Operation time of LAVH group was significantly more longer than TVH group, 173.21 mins vs 127.36 mins respectively (p<0.001), and postoperative discharge day of LAVH group was more longer than TVH group, 5.59 days vs 4.86 days respectively (p=0.034). But there was not statistically significant difference between TVH group and LAVH group in relation to others: age, weight, parity, previous abdominal operation, uterine weight, combined operation, postoperative hemoglobin change, gas out day, and complications. Conclusion : Both LAVH and TVH have many following advantages compared with abdominal hysterectomy: less pain, shorter hospital day, cosmetic advantages, and lower mobidity. TVH even can be done safely in cases of large uterus or previous abdominal operation, but disadvantages of that are limited operation field and no visible abdominal cavity. In comparison, LAVH offers a view of abdominal cavity and can perform adnexal operation or adhesiolysis, but disadvantage of that is high cost. Thus the study in relation to appropriate selection of hysterectomy will be necessary to satisfy patient and lower cost.

간경화를 가진 환자의 척추 수술 후 발생한 급성 간부전 : 증례보고 A case report

김형남,최준석,박천희,이철승,김원태 全南大醫大 2001 전남의대학술지 Vol.37 No.4

Chitin deacetylase from Absidia coerulea CHK-1 : Mycerial chitin deacetylase of a chitin deacelylase-hyperproducuing fungus, Absidia coerulea CHK-1

Park, Chun,Kim, Jung Ran,Shin, Jae Kyoung,Kim, June Ki,Lee, Tae Kyun,Chung, Ji Chun,Park, Weon Hwan,Park, Sun Dong,Nam, Kyung Soo,Lee, Young Choon,Kim, Cheorl Ho 한국키틴키토산학회 1997 한국키틴키토산학회지 Vol.2 No.2

A mycelial chitin deacetylase has been purified from a chitin deacetylase-hyperproducing fungus, Absidiα coerulea CHK-1. The purified enzyme had a molecular mass of about 62 kDa on denaturated and natural conditions. The pI was 5.5. The chitin deacetylase, when resolved by SDS-PAGE, was positive for Schiff staining, suggesting that the enzyme is a glycoprotein. When O-hydroxylated chitin (glycolchitin) was used as a substrate, the enzyme displayed a temperature optimum of around 50℃ and a pH optimum of around PH 5,5. The enzyme was stable to incubation from pH 3.0 to pH 6.5 at 4℃ for 24 hr. The presence of chitin protected the enzyme from heat inactivation, the extent depending upon the substrate concentration. The activity of the enzyme was stimulated by Mn2+ ion. The enzyme is active on chitooligosaccharides with more than two N-acetylglucosamine residues (M-acetylchitobiose). However, the enzyme is not active on N-acetylglucosamine. The enzyme had an apparent Km of 12.4 mM and Kcat of 32.4 /sec for glycol chitin, respectively.

FYN promotes mesenchymal phenotypes of basal type breast cancer cells through STAT5/NOTCH2 signaling node

Lee, Ga-Hang,Yoo, Ki-Chun,An, Yoojeong,Lee, Hae-June,Lee, Minyoung,Uddin, Nizam,Kim, Min-Jung,Kim, In-Gyu,Suh, Yongjoon,Lee, Su-Jae Nature Publishing Group UK 2018 Oncogene Vol.37 No.14

<P>Basal type breast cancer is the most aggressive and has mesenchymal features with a high metastatic ability. However, the signaling node that determines the basal type features in breast cancer remains obscure. Here, we report that FYN among SRC family kinases is required for the maintenance of basal type breast cancer subtype. Importantly, FYN enhanced NOTCH2 activation in basal type breast cancer cells through STAT5-mediated upregulation of Jagged-1 and DLL4 NOTCH ligands, thereby contributed to mesenchymal phenotypes. In addition, we found that high levels of FYN persist in basal type breast cancer cells by a positive feedback loop between FYN and STAT5. FYN interacted directly with STAT5 and increased p-STAT5 that further acts as a transcription factor for FYN. Taken together, our findings demonstrate a pivotal role of FYN and its downstream effectors in maintaining the basal type features in breast cancer.</P>

백혈병 세포에서 Multidrug Resistance Gene-1 (mdr1)의 과발현이 99mTc-sestaMIBI 섭취에 미치는 영향

이상우,천경아,강도영,전수한,정준기,이규보,손상균,이재태,이종기 대한핵의학회 1999 핵의학 분자영상 Vol.33 No.2

Purpose: To determine whether Tc-99mMIBI is recognized by the multidrug resistant P-glycoprotein (Pgp), we have measured quantitatively Tc-99mMIBI uptake in cancer cells. The effects of various Pgp reversing agents on cellular Tc-99m-MIBI uptake were also investigated in the presence of multidrug resistance gene-1 (mdr1 gene) overexpression. Materials and Methods: We measured percentage uptake of Tc-99m-MIBI at different incubation temperatures both in mdr1 positive and negative cells. The effects of verapamil, cyclosporin, and dipyridamole on cellular uptake of Tc-99m-MIBI were also evaluated with or without overexpression of mdr1 gene in cultured murine leukemia L1210 cells. Results: The mdr1 gene expressing cell lines were effectively induced in in vitro with continuous application of low-dose adriamycin or vincristine. Cellular uptake of Tc-99m-MIBI was higher in mdr1 negative L1210 cells than those of mdr1 positive cells, and higher when incubated in 37℃ than 4℃. In the presence of verapamil, cyclospor or dipyridamole, Tc-99m-MIBI uptake was increased upto 604% in mdr1 positive cells. Conclusion: Cellular uptake of Tc-99m-MIBI is lower in leukemia cells over-expressing mdr1 gene, and MDR-reversing agents increase cellular uptake. These results suggest that Tc-99m-MIBI can be used for characterizing Pgp expression and developing MDR-reversing agents In vitro.

Reliable Multivalued Conductance States in TaO_<i>x</i> Memristors through Oxygen Plasma-Assisted Electrode Deposition with in Situ-Biased Conductance State Transmission Electron Microscopy Analysis

Lee, Myoung-Jae,Park, Gyeong-Su,Seo, David H.,Kwon, Sung Min,Lee, Hyeon-Jun,Kim, June-Seo,Jung, MinKyung,You, Chun-Yeol,Lee, Hyangsook,Kim, Hee-Goo,Pang, Su-Been,Seo, Sunae,Hwang, Hyunsang,Park, Sung American Chemical Society 2018 ACS APPLIED MATERIALS & INTERFACES Vol.10 No.35

<P>Transition metal oxide-based memristors have widely been proposed for applications toward artificial synapses. In general, memristors have two or more electrically switchable stable resistance states that device researchers see as an analogue to the ion channels found in biological synapses. The mechanism behind resistive switching in metal oxides has been divided into electrochemical metallization models and valence change models. The stability of the resistance states in the memristor vary widely depending on: oxide material, electrode material, deposition conditions, film thickness, and programming conditions. So far, it has been extremely challenging to obtain reliable memristors with more than two stable multivalued states along with endurances greater than ∼1000 cycles for each of those states. Using an oxygen plasma-assisted sputter deposition method of noble metal electrodes, we found that the metal-oxide interface could be deposited with substantially lower interface roughness observable at the nanometer scale. This markedly improved device reliability and function, allowing for a demonstration of memristors with four completely distinct levels from ∼6 × 10<SUP>-6</SUP> to ∼4 × 10<SUP>-8</SUP> S that were tested up to 10<SUP>4</SUP> cycles per level. Furthermore through a unique in situ transmission electron microscopy study, we were able to verify a redox reaction-type model to be dominant in our samples, leading to the higher degree of electrical state controllability. For solid-state synapse applications, the improvements to electrical properties will lead to simple device structures, with an overall power and area reduction of at least 1000 times when compared to SRAM.</P> [FIG OMISSION]</BR>

Mutalisk: a web-based somatic MUTation AnaLyIS toolKit for genomic, transcriptional and epigenomic signatures

Lee, Jongkeun,Lee, Andy ,Jinseok,Lee, June-Koo,Park, Jongkeun,Kwon, Youngoh,Park, Seongyeol,Chun, Hyonho,Ju, Young Seok,Hong, Dongwan Oxford University Press 2018 Nucleic acids research Vol.46 No.w1

<P><B>Abstract</B></P><P>Somatic genome mutations occur due to combinations of various intrinsic/extrinsic mutational processes and DNA repair mechanisms. Different molecular processes frequently generate different signatures of somatic mutations in their own favored contexts. As a result, the regional somatic mutation rate is dependent on the local DNA sequence, the DNA replication/RNA transcription dynamics and epigenomic chromatin organization landscape in the genome. Here, we propose an online computational framework, termed Mutalisk, which correlates somatic mutations with various genomic, transcriptional and epigenomic features in order to understand mutational processes that contribute to the generation of the mutations. This user-friendly tool explores the presence of localized hypermutations (<I>kataegis</I>), dissects the spectrum of mutations into the maximum likelihood combination of known mutational signatures and associates the mutation density with numerous regulatory elements in the genome. As a result, global patterns of somatic mutations in any query sample can be efficiently screened, thus enabling a deeper understanding of various mutagenic factors. This tool will facilitate more effective downstream analyses of cancer genome sequences to elucidate the diversity of mutational processes underlying the development and clonal evolution of cancer cells. Mutalisk is freely available at http://mutalisk.org.</P>