Inositol-Hexaphosphoric Acid의 피부노화방지

조춘구,홍세흠 崇實大學校 生産技術硏究所 2002 論文集 Vol.32 No.-

The anti-skinaging effects on woman's skin were investigated with inositol-hexaphosphoric acid(IP_6). The concentrations (wt%) of IP_6 0, 0.1, 0.2, 0.4, 0.8, 1.0. The IP_6-skin lotions were applied on the arms and face of the subjects everyday for 7 weeks. The moisture, lightening, sebum, elasticity and wrinkle of the skin were measured. The experimental instruments were corneometer, sebumeter, autometer, mexameter. MX-16 and image analyzer. It showed that IP_6 substantially improved the subject's skin condition. The moisture value changed from 47 to 89, the sebum values of dry and oily skins changed close to that of normal skins, lightening of the skin changed from 490 to 461, elasticity changed from 0.118 to 0.134, and the improvement on wrinkle was 12.9%. With the experimental results the 0.4-IP_6 skin lotion showed the best results.

오존과 염소에 의한 대장균 살균에 미치는 온도의 영향

방기인,조춘구 崇實大學校 生産技術硏究所 1996 論文集 Vol.26 No.1

In the drinking water treatment, chlorine has been used the most popular for disinfection last 100 years. Chlorination has not only disinfection effect but also oxidation of matgnesium and iron in the water. However, chlorine also reacts with natural organic matter in water to produce disinfection by-products such as trihalomethane and haloacetic acid which are recognized as carcinogenic materials. Therefore, alternative disinfection method has been developed like ozonation and UV radiation. The ozonation is one of the most popular method to achieve the same goal as chlorination in the drinking water treatment. In the chlorination, various chlorine was used from 0.05 to 2.0mg/L with 30 to 60 seconds contact time. After 30 seconds cotact time with 1mg/L and of chlorine, 99% of Escherichia coli was killed while 0.5mg/L of chlorine was used with 60 seconds contact time. To meet the drinkin water regulation 1mg/L and 0.5mg/L of chlorine shold be used for 30 and 60 seconds, respectively. For ozonation, 0.05 to 1.56mg/L of ozone was used. For ozonation, 0.26mg/L of ozone was used with 20℃ while 1.04mg/L of ozone with 4℃ for 99.7% E. coli disinfection.

유기 과산화물이 Polypropylene의 유동 특성에 미치는 영향

윤홍식,최병오,조춘구 崇實大學校 1988 論文集 Vol.18 No.-

The Effects of Inositol Extracted from Rice on the Skin

( Choon Koo Zhoh ),( Hwan Song ),( Chang Giu Han ),( Fumi Tsuno ) 대한화장품학회 2001 대한화장품학회지 Vol.27 No.1

Inositol, is a water-soluble crystalline compound. It helps with people’ s metabolism and decreases cholesterol levels. It is also known to have anti-cancer results. In order to find out the affects of Inositol on the skin, inositol skin lotion was produced with each amount of Inisitol: 0, 0.5, 1.0, 2.0, 3.0wt% and tested on the faces and the arm areas of women in all ages for 7 weeks. The moisture, sebum, change in elasticity, and improvement of wrinkles were measured. Corneometer, Sebumeter, Cutometer, and an image analyzer were used as measuring equipments. There are subtle differences in the subjects when 1-2% of Inositol is used the moisture of the skin improved 19%, elasticity by 17%, and the amount of sebum for dry and oily skin types adjusted to the amount of sebum of the neutral skin types. This influenced the length, width, the number of peak, and the height of the wrinkles to improve 12.4%. It is thought that Inositol would be an effective new raw material in cosmetics.

The Effect of Obesity Management Program of Adiponectin and homocysteine Level in Serum in Obese Women in Korea

Choon-Koo Zhoh,Eun-Ju Lee,Jung-Soon Han 한국식품영양과학회 2011 한국식품영양과학회 학술대회발표집 Vol.2011 No.10

나노세라마이드의 캡슐화와 아토피 피부의 치료

조춘구 ( Choon-koo Zhoh ),김인영 ( In-young Kim ),이희섭 ( Hee-seob Lee ) 대한화장품학회 2004 대한화장품학회지 Vol.30 No.3

나노 세라마이드의 캡슐화(Nano-ceramide capsulation)는 세라마이드 III와 토코페릴리놀레이트를 나노 크기의 모노 베지클에 캡슐화시켜 피부각질층에 작용하도록 만든 기술이다. 그 제조에는 고압 마이크로플루다이저를 사용하였으며 조성은 다음과 같다. 모노 베지클의 막 강화제로서 0.5~5.0 wt%의 수소첨가레시친과 0.1~2.0 wt%의 리소레시친을 사용하였으며, 용제로서 5.0~10.0 wt%의 propylene glycol과 5.0~10.0 wt%의 ethanol을 사용하였다. 피부 보습기능과 아토피 치료를 위하여 활성성분인 세라마이드 III와 토코페릴리놀레이트를 사용하였으며 유화제는 함유하지 않았다. 나노 세라마이드 캡슐기술의 최적조건은 다음과 같다. 마이크로플루다이저의 통과압력은 1,000 bar, 통과횟수는 3회, 통과 온도는 60~70℃가 적당하였다. 또한, nano capsule의 pH는 5.8±0.5이었다. 평균입자크기는 63.1±7.34 nm로 물과 같은 투명한 성상을 보였으며, 제타포텐셜값은 -55.1±0.84 mV이었다. 임상실험 결과로서, 피부보습효과(in-vivo, n=8, p-value<0.05)는 비교시료보다 21.15% 개선되었으며, 치료 전보다는 36.31% 개선되었다. 더구나, 아토피 피부 효과는 아토피 피부 환자 10명에게서 양성반응을 보였다. The nano-ceramide capsulation is a technique that capsulates ceramide III and tocopheryl linoleate at the mono-vesicle to act on the horny layer in skin. In this technique, 0.5~5.0 wt% of hydrogenated lecithin and 0.01~2.00 wt% of lysolecithin are used as the membrane-strengthen agents of the mono-vesicle and 5.0~10.0 wt% of propylene glycol and 5.0~10.0 wt% of ethyl alcohol are used as solvents. Active ingredients such ceramide III and tocopheryl linoleate are utilized to enhance the moisturizing efficacy and treat atopy skin. These materials do not contain synthetic emulsifiers. The optimal conditions or nano-ceramide capsulation are such that particles pass Microfludizdizer 3 times at 1,000 bar and 60~70℃ and pH of nano capsules is 5.8±0.5. The average size of particles is 63.1±7.34 nm showing lucid state like water by the laser light scattering. A zeta potential value is -55.1±0.84 mV. Through clinical tests, the moisturizing effect (in-vivo, n=8, p-value<0.05) showed 21.15% of improvement comparison to comparison-samples and 36.31% of improvement compared to the state before treatment. Moreover, the effectiveness of atopy skin showed positive reaction from 10 volunteers.

제미니형 계면활성제를 사용한 액정기술의 제조방법

조춘구 ( Choon-koo Zhoh ),김인영 ( In-young Kim ),한창규 ( Chang-giu Han ) 대한화장품학회 2004 대한화장품학회지 Vol.30 No.3

제미니형 계면활성제를 사용하여 액정을 제조하였으며 보습효능을 측정하였다. 계면활성제로는 디코코일에틸렌디아민(PEG) -15설페이트 (SCD-PEG-15S) 3.0 wt%와 부스터로는 수소첨가 다이머에씨드에스터(HDAE) 4.0 wt%를 사용하였다. 안정화제로 베헤닐알코올(BA) 2.0 wt%와 리소레시친(LyL) 1.0 wt%를 사용하였다. pH 4.0~10.5 범위에서 안정하였으며 최적조건은 6.5이었다. 가장 안정한 상태의 점도는 8,000±500 cP이었으며. 입자크기는 4.0에서 15.5 μm 범위에서 가장 잘 형성되었다. 입자의 모양과 구조는 편광현미경을 통하여 관찰하였으며, LC의 입자 주변에 액정이 형성되었음을 확인하였다. 또한 액정의 입자주변에 겔 네트워크 구조를 형성하고 있다는 것을 알 수 있었다. 시료도포 후 30 min 경과후의 보습효과는 control보다 13.6% (*P<0.05) 증가함을 보였으며, 1 h 후의 보습효과는 control보다 12.6% (*P<0.05) 증가함을 보였다. 또한 4 h 후의 보습효과는 control보다 28.3% (P<0.05) 증가하였다. 이는 제조한 액정이 라멜라형 구조를 형성하여 수분 포접력과 오일의 흡착력 증가로 인한 효과로 판단된다. 향후 피부의 전달체계(delivery system)에의 적용이 가능하고 나아가 의약이나 제약, 화장품 등을 만드는데 응용될 것으로 기대된다. In this study, liquid crystalline (LC) is formed using Gemini surfactant (GS) type and moisturizing effect in vivo is measured. 3.0 wt% of sodium dicocoyl ethylene diamine (PEG)-15 sulfate (SCD-PEG-15S) is used as GS and 4.0 wt% of hydrogenated dimer acid esters (HDAE) as booster. For stabilizers, 2.0 wt% of behenyl alcohol (BA) and 1.0 wt% of lyso-lecithin (LyL) are utilized. It is stabilized in pH from 4.0 to 10.5 and the best condition is in pH 6.5. The value of viscosity is 8,000 ± 500 cP. The most excellent particles are formed within the range of 4.0 to 15.5 um. Formed LC is observed around LC particles using polarization microscope. It is also observed that lamellar gel network structure is formed around LC particles. Moisturizing effect is improved by 13.6% (*P<0.05) compared to control when measured 30 min later after coating samples. After 1 h, moisturizing effect is improved by 12.6% (*P<0.05) than control while showing 28.3% (*P<0.05) of improvement after 4 h. These results may be caused from that manufactured LC forms lamellar structure so that it has better water-holding ability and absorbance of oil increases. This formula could be utilized by delivery system (DS) on skin so that this technology can be applied for manufactuing pharmaceuticals and cosmetics.

이노시톨과 피틴산이 피부에 미치는 영향

조춘구 ( Choon Koo Zhoh ),한창규 ( Chang Giu Han ),송환 ( Hwan Song ) 대한화장품학회 2004 대한화장품학회지 Vol.30 No.4

쌀눈에서 추출한 inositol과 phytic acid를 화장품에 적용하기 위해 피부에 미치는 영향에 대해 연구하였다 20~40대의 아시안 여성 45명을 대상으로 7주간 inositol 0~3.0 wt%와 phytic acid 0~1.50 wt%가 함유된 스킨로션을 제조, 사용 후 피부의 수분, 유분, 탄력, 주름에 미치는 영향에 대해서 알아보았다 Inositol 1.0 wt%가 함유된 스킨로션을 사용한 경우 피부의 수분 함유량은 19% 증가하였으며, 주름은 12.4% 개선되었고, 피부탄력은 12.4% 증가되었다. 또한 phytic acid 0.50 wt%를 함유한 스킨 로션을 사용한 경우 피부의 수분은 71.6% 증가하였으며, 주름은 16.9% 감소하였고, 탄력은 21.9%가 증가하였다. Inositol과 phytic acid를 지성과 건성피부에 적용한 결과 2~4주 후면 정상피부의 유분량으로 조절됨을 알 수 있었다 Inositol과 phytic acid는 모두 피부개선에 효과적이나, phytic acid는 민감한 피부에는 적용하기 어려운 점이 있다. 따라서 1.0 wt%의 inositol에 0~0.5 wt%의 phytic acid를 첨가한 스킨로션을 제조하여 그 효과에 대해서 알아보았다. 이 경우 1.0 wt%의 phytic acid에 1.0 wt%의 inositol을 사용한 경우 수분은 63.8%, 탄력은 17.2% 증가하였으며, 주름은 17.4% 개선되었고, 피부 유분량은 약 2주 후에 정상적인 상태로 개선됨을 알 수 있었다. 따라서 inositol 1.0 wt%에 0.10 wt%의 phytic acid를 첨가하여도 피부 개선효과가 나타남을 알 수 있다. 본 연구의 결과 내에서 1.0 wt%의 phytic acid와 1.0 wt%의 inositol을 사용한 경우 적은 양으로도 피부 개선 효과가 우수한 상승효과가 나타남을 알 수 있었다. Inositol and phytic acid extracted from rice bran were investigated for applying cosmetics. Skin lotions containing 0~3.0 wt% inositol and 0~1.50 wt% phytic acid were applied respectively, to the arm skins of 45 Asian women 20'~40's for 7 weeks. Improvement on moisture was evaluated. In addition, improvements on sebum, elasticity, and wrinkle were examined after applying placebo, inositol and phytic acid-containing skin lotions tot face, respectively. For 1.0 wt% inositol, it resulted in 19% increase of moisture. The wrinkle reduction and elasticity improved 12.4% and 17.0% on average, respec-tively. Applying 0.5 wt% phytic acid resulted in 71.6% increase the moisture. Improvements on wrinkle and elasticity were 16.9% and 21.9% respectivelv. hpplving inositol or phytic acid regardless of dry or oily skin, resulted in sebum value recovery to that of the normal skin after 2~4 weeks. Although inositol is inferior to phytic acid in improvements of the skin, phytic acid is not suitable to sensitive skin. So, 0~0.50 wt% of phytic acid were added to 1.0 wt% inositol and similar experiments were carried out. In case of added 1.0 wt% phytic acid, moisture increased 63.8% approximately. Improvements on elasticity and reduction on wrinkle were 17.2% and 17.4%, respectively. Both skin types were turned to normal skin type after 2 weeks. It could improve the skin condition when used inositol added phytic acid. The optimized concentration of phytic acid was 0.10 wt% with 1.0 wt% of inositol without side effect.

고급알코올과 고급지방산이 O/W에멀젼의 유동특성에 미치는 영향

조춘구 ( Choon Koo Zhoh ),이강연 ( Kang Yen Lee ),김동남 ( Dong Nam Kim ) 대한화장품학회 2009 대한화장품학회지 Vol.35 No.2

Oil in water (O/W)에멀젼에서 에멀젼 안정화제로 사용되는 고급알코올과 고급지방산이 O/W에멀젼의 유동특성에 미치는 영향을 점도와 rheograms으로 분석하였다. 고급알코올과 고급지방산의 탄소 체인 길이가 증가할수록 O/W 에멀젼의 점도가 상승하는 경향을 나타내었으며 고급알코올의 점도 상승 효과가 고급지방산보다 우수하였다. 또한 에멀젼 제조에 사용된 계면활성제의 친유부의 탄소 체인 길이에 유사한 스테아릴알코올과 세테아릴알코올의 점도 상승 효과가 가장 우수하였다. 고급알코올과 고급지방산이 첨가된 O/W에멀젼은 화장품 에멀젼이 보여주는 유동특성인 shear thinning을 나타내는 pseudo-plastic유체였다. 세틸알코올, 세테아릴알코올과 스테아릴알코올이 첨가된 O/W에멀젼은 thixotropy이었으며 농도가 증가할수록 thixotropic 현상이 증가하는 경향을 나타내었다. 또한 고급지방산보다는 고급알코올이 첨가된 O/W에멀젼이 더 thixotropic한 유동특성을 나타내었다. O/W에멀젼의 안정성과 관련된 점도상승과 사용감과 관련된 spreadability 향상을 위해 에멀젼 안정화제로 고급지방산보다는 고급알코올의 사용이 적합하리라 생각된다. The objective of this study is to analyze the influences of fatty alcohols and fatty acids on rheological properties of oil in water (O/W) emulsions using viscosity and rheograms. As the chain length of fatty alcohols and fatty acids lengthened, the viscosity of emulsions was increased. The influence of fatty alcohols on viscosity enhancement was stronger than that of fatty acids. Both stearyl alcohol and cetearyl alcohol, which have carbon chain length similar to lipophilic portion of surfactant used in emulsion preparation, had showed the best increase in viscosity of O/W emulsions. O/W emulsions prepared with fatty alcohols and fatty acids were pseudo-plastic fluid and they showed shear thinning behaviour like as the common cosmetic emulsions. O/W emulsions prepared with cetyl alcohol, cetearyl alcohol and stearyl alcohol were thixotropic fluids and thixotropy increased with an increase in the concentration of fatty alcohols and fatty acids. Also O/W emulsions prepared with fatty alcohols were more thixotropic than those prepared with fatty acids. For the sake of viscosity increase related to O/W emulsions stability and spreadability enhancement related to payoff, it is thought that fatty alcohols are more useful than fatty acids in the O/W emulsions as the emulsion stabilizer.

Retinol에 대한 항산화 연구

조춘구 ( Choon Koo Zhoh ),한창규 ( Chang Giu Han ),흥우진 ( Woo Jin Hong ) 대한화장품학회 2002 대한화장품학회지 Vol.28 No.1

Retinol에 대한 합성물질과 천연물질의 항산화력을 비교하였다. 수용성 합성 항산화제 tertiary butylhydroquinone(TBHQ)와 수용성 천연 항산화제 a-glycosyl rutin(a-G rutin), licorice, pycnogenol, 지용성 합성 항산화제 butylated hydroxytoluene(BHT)와 지용성 천연 항산화제 a-lipoic acid, ferulic acid, natural concentrated tocopherol(nc-tocopherol)를 각각 0.0, 0.01, 0.02, 0.05, 0.10wt% 사용하여 리포좀에 봉입하였으며, 4°C, 25°C, 50°C 에서 8주 동안 retinol의 잔류량 변화를 HPLC로 측정하였다. 사용된 항산화제는 retinol에 대하여 모두 산화억제효과를 나타내었으며, 수용성 항산화제는 licorice >pycnogenol >TBHQ >a-G rutin 순으로, 지용성 항산화제는 a-lipoic acid >BHT>nc-tocopherol >ferulic acid 순으로 나타났다. 또한 a-lipoic acid,BHT, licorice, pycnogenol을 혼합하여 retin이에 대한 항산화력 상승효과를 알아보았다. a-lipoic acid와 BHT를 혼합하여 사용하였을 때 retinol에 대한 항산화력이 가장 좋게 나타났다. In an attempt to compare the antioxidation effects of constrain the oxidation and improve the structural stability, retinol and various antioxidants were together encapsulated by liposome. Four water soluble and four oil soluble antioxidants were tested for performance. The influence of tertiary butylhydroquinone(TBHQ), α-glycosyl rutin(α-G rutin), licorece, pycnogenol as water soluble antioxidants and butylated hydroxytoluene(BHT), α-lipoic acid, ferulic acid, natural concentrated tooopherol(no-tocopherol) as oil soluble antioxidants on the constraint of oxidation of retinol were investigated. Additional study was conducted to compare the synergic effect of antioxidation for retinol with licorice, pycnogenol, α-lipoic acid and BHT. All the antioxidant used at the study constrained oxidation of retinol. The effect of antioxidation for retinol increased in order of licorice, pycnogenol, TBHQ, α-G rutin as water soluble antioxidants and α-lipoic acid, BHT, no-tocopherol, ferulic acid as oil soluble antioxidants. In conclusion, α-lipoic acid is more effective retinol antioxidants than BHT. And the combination of α-lipoic acid and BHT gave best synergic among six combinations.