Helicobacter pylori와 대장균의 Shuttle Vector 개발

조명제,이우곤,이상룡,김경희,안영숙,김성희,김현주,류복덕,최여정,윤영혜,백승철,전영석,이광호 경상대학교 유전공학연구소 1997 遺傳工學硏究所報 Vol.16 No.-

In this study, a vehicle vector using cryptic plasmids was constructed for gene transfer in Helicobacter pylori. pHP51(3.9 kb) and pHP489(1.2 kb) were selected for constructing vectors from cryptic plasmid of H. pylori isolates in Korea. The HindⅢ-digestedDNA fragment(1.2kb) of pHP489 and 1.6kb DNA fragment of pHP51 were ligated with a kanamycin resistance gene(aph3'-Ⅲ) from C. jejuni to produce the recombinant plasmids pHP489K and pHP51K, respectively. Transformation frequency of pHP51K by electroporation was low. But pHP489K could be effectively transformed into various H. pylori strains. In order to design an intermdiate vehicle vector for gene transfer into H. pylori, pBlueHP489K was prepared by recloning pHP489K DNA into pBluescript and pTZ19R vector. This vector permitted the DNA fragment containing pHP489 sequence, aph3'-Ⅲ, and cloned DNA to be cut and self-ligated in the SacⅠ site after cloning. ureA and ureB gene were inserted into pBlueHP489K, resulting in pBlueHP489K/AB. The DNA fragment containing pHP489, kanamycin resistance gene(aph3'-Ⅲ), and urease structural gene was cut away from pBlueHP489K/AB and self-ligated to generate pBlueHP489K/AB. pBlueHP489K/AB made urease-negative H. pylori strains restore their urease activity. By this experiment, pBlueHP489K was confirmed to be the vehicle system for transferring H. pylori genes.

연령집단에 따른 노인의 허약(Frailty) 예측요인 분석

조성은 ( Cho Sung Eun ),최은영 ( Choi Eun Young ),오영삼 ( Oh Young Sam ),김영선 ( Kim Young Sun ),김성복 ( Kim Seong Bok ) 한국보건사회연구원 2017 保健社會硏究 Vol.37 No.3

본 연구의 목적은 연령집단에 따른 허약의 예측요인을 분석하는 것이다. 연구의 목적을 위해 2014년 노인실태조사를 사용하여 총 4,123명을 분석의 대상으로 설정하였다. 허약을 예측하는데 있어 연령의 중요성을 고려하여, 본 연구는 대상자를 75세 이상 85세 미만의 중 고령노인집단(n=3,373명)과 85세 이상의 초 고령노인집단(n=750명)으로 분류하여 연구모형을 검증하였다. 중 고령노인집단에서 전 허약노인과 허약노인은 각각 1453명(약 43%), 1,268명(약 37%)으로 나타났으며, 초 고령노인 집단에서 전 허약과 허약노인은 304명(약 40%), 396명(약 52%)으로 나타났다. 회귀분석의 결과 건강단계에서 허약전 단계로 진입하는 예측요인은 중 고령노인집단에서는 여성, 낮은 교육수준, 많은 만성질환, 낙상 유경험자, 높은 우울, 낮은 인지기능이 허약 전 단계를 예측하는 유의한 변인으로 밝혀졌으며 초 고령노인집단은 여성, 높은 우울, 낮은 사회활동이 유의한 예측요인으로 밝혀졌다. 허약 전 단계에서 허약 단계로의 진입을 예측하는 요인으로는 중 고령노인집단은 여성, 높은 연령, 미취업, 많은 만성질환, 낙상 유경험자, 높은 우울, 낮은 인지기능이 유의한 변인이었으며, 초 고령노인 집단은 높은 연령, 많은 만성질환, 높은 우울, 낮은 인지기능이 유의한 예측요인으로 밝혀졌다. 본 연구는 연령의 세분화와 다각적인 요인의 접근으로 허약의 예측요인을 밝히고자 하였다. 연구를 통해 밝혀진 예측요인은 추후 허약에 대한 다각적 연구의 기초자료로서 활용되리라 기대한다. This study aims to investigate the predictors of frailty in different age groups. To address this purpose, this study used the 2014 Survey of the Living Conditions of the Elderly (SLCE). In the research model, 4,123 older adults aged 75 and older were included from the SLCE. All participants were subdivided into two age groups based on their age; the young old group (75-85) and the old-old group (85+). The young old group consisted of 1,453 (43%) pre-frail older adults and 1,268 (37%) frail older adults; the old-old group consisted of 304 (40%) pre-frail older adults and 396 (52%) frail older adults. Our regression analysis showed that in the young-old, being female, lower levels of education, having more chronic diseases, having fall experience, higher depression, lower cognitive functioning significantly predicted transition to pre-frailty from non-frail status. In the old-old, being female, higher depression, lower level of social activity were significant predictors. On the other hand, predictors of transition to frailty from pre-frail status in the young-old were being female, higher age, being unemployed, having more chronic diseases, having fall experience, higher depression, lower cognitive functioning. In the old-old, predictors of transition to frailty from pre-frail status were only higher age, having more chronic disease, higher depression, and lower cognitive functioning. The research findings can be used as a reference point in the making of strategies for preventing frailty.

미세유화약물송달시스템을 이용한 로바스타틴의 생체이용률 향상

윤복영,강복기,정상영,이영원,이시범,황성주,육순홍,강길선,이해방,조선행 한국약제학회 2002 Journal of Pharmaceutical Investigation Vol.32 No.4

A self-microemulsifying drug delivery system (SMEDDS) was developed to increase the dissolution rate, solubility, and ultimately bioavailability of a poorly water soluble drug, lovastatin. SMEDDS was the mixtures of oils, surfactants, and cosurfactants, which emulsify under conditions of gentle agitation, similar to those which would be encountered in the gastro-intestinal (GI) tract. Various types of self-emulsifying formulations were prepared using four types of oil (Capryol 90, Lauroglycol 90, Labrafil M 1944 CS and Labrafil M 2125), two surfactants (Cremophor EL and Tween 80), and three cosurfactants (Carbitol, PEG 400 and propylene glycol). The efficiency of emulsification was studied using a laser diffraction size analyzer to determine particle size distributions of the resultant emulsions. Optimized formulations selected for bioavailability assessment were Carpryol 90 (40%), Cremophor EL (30%) and Carbitol (30%). SMEDDS containing lovastatin (20 mg and 5 mg) were compared to a conventional lovastatin tablet (Mevacor^? , 20 mg/tab) by the oral administration as prefilled hard gelatin capsules to fasted beagle dogs for in vivo study. The area under the serum concentration-time curve from time zero to the last measured time in serum, AUC_0→24h, was significantly greater in SMEDDS, suggesting that bioavailability increase 130% and 192% by the SMEDDS, respectively. The self-emulsifying formulations of lovastatin afforded the improvement in absolute oral bioavailability relative to previous data of lovastatin tablet formulation. These data indicate the utility of dispersed self-emulsifying formulations for the oral delivery of lovastatin and potentially other poorly absorbed drugs.

임신기간중 흰쥐 자궁내막의 Lectin 결합특성에 대한 조직화학적 연구

백상호,김복린,손현준,유영복,이경훈,이화영,이영기,박경한,조사선 대한해부학회 1995 Anatomy & Cell Biology Vol.28 No.5

간호실무에서 신봉이론(Espoused theories)에 대한 탐색적 연구

서문자,김혜숙,이은희,박영숙,조경숙,강현숙,임난영,김주현,이소우,조복희,이명하,지성애,하양숙,손영희,권성복,김희진,추진아 성인간호학회 2001 성인간호학회지 Vol.13 No.1

As a nursing practice involves nurses' actions in a specific context of health care, this study has focused on exploring the espoused theories in nursing practice within the action science perspectives, Espoused theories are the belief, principles, and rationale expressed by the practitioner as guiding her/his actions in a situation of practice. The data were analysed qualitatively and 25 elements of espoused theories of nursing action were identified and clustered into 6 categories. The 25 elements of espoused theories are as follows: The clinical nurse worked in wholistic and individual nursing, focussed on the patient's needed, comfort and supportive nursing (5 theories of nursing goal) ; excellent skills, knowledge based, assessment and data collection, explaining, educating or a scientific basis(6 theories of nursing intervention): advocacy, value oriented, treatment, account- ability and commitment(4 theories of nursing ethics) ; human respect. partnership, trust(3 theories of patient-nurse relationship) : knowledgable, accumulated clinical experiences and personally lived experiences. positive perspectives(4 theories of nurse), role of intervention. rewarding peer relationship(3 theories of situations) The above mentioned espoused theories are similar to that of nursing textbooks which students learned through basic nursing education and almost the same as the Acts ofa Nurse in Korean. However, we are doubtful whether nurses actually do as they think. Therefore. it is recommended to review the theories-in-use in order to and any discrepancies between the espoused theories and the reality of nursing actions

니세틸 정(아세틸-엘-카르니틴 500 mg)에 대한 뉴로세틸 정의 생물학적 동등성

조혜영,김은아,정현철,심영순,임동구,오인준,문재동,이용복 전남대학교 약품개발연구소 2001 약품개발연구지 Vol.10 No.-

Acetyl-L-carnitine (ALC), an endogenous component of the L-carnitine family, is naturally occurring molecule synthesized from L-carnitine (LC) by carnitine acetyl transferase. ALC has been shown to improve the cognitive performance of patients suffering from dementia of the Alzheimer's type and proposed for treating Alzheimer's disease in pharmacological doses. The purpose of the present study was to evaluate the bioequivalence of two ALC tablets, Nicetiler^TM (Dong-A pharmaceutical Co., Ltd.) and Neurocetil^TM (Kyung-Dong Pharmaceutical Co., Ltd.), according to the guidelines of Korea Food and Drug Administration. Twenty six normal male volunteers, 22.80±2.76 year in age and 63.07 7.98㎏ in body weight, were divided into two groups and a randomized 2×2 cross-over study was employed. After one tablet containing 500㎎ of ALC was orally administered, blood was taken at predetermined time intervals and the concentrations of ALC in serum were determined using HPLC with fluorescence detector. Because of the presence of endogenous ALC, the calibration was performed using dialyzed serum. Pharmacokinetic parameters such as AUC_t, C_max and T_max were calculated and ANOVA was utilized for the statistical analysis of the parameters. The results showed that the differences in AUC_t, C_max and T_max between two tablets were 2.72%, -0.65% and -8.42%, respectively, when calculated against the Nicetile^TM tablet. The powers (1-β) for AUC_t and C_max were 94.87% and 87.17%, respectively. Minimum detectable differences (Δ) at α=0.05 and 1-β=0.8 were less than 20% (e.g., 15.58% and 19.16% AUC_t and C_max, respectively). The 90% confidence intervals were within ±20% (e.g., -11.84∼6.41 and -10.57∼11.88 for AUC_t and C_max, respectively). Two parameters met the criteria of KFDA for bioequivalence, indicating that Neurocetil^TM tablet is bioequivalent to Nicetile^TM tablet.

1920년대 초기 사회주의 사상가들의 시와 그 성격

조영복 우리말글학회 2001 우리말 글 Vol.21 No.-

The purpose of the present essay lies in elucidating the poetry of socialist activity and its characteristics in the early 1920's. In those days many literary magazines were founded and through them many people accepted socialist way of thinking and the diverse trend of literary thoughts. Social activists who devoted themselves to anarchism communism represented their poems on the magazines, the Changmich'on, the Daijungshibo, the Pyeheo etc. The poets who participated actively in these magazines were Jung Tai-shin, Bosuns, Hwang Seog-u. etc. The character of these magazines was that social activists weren't distinguished from writers or poets. Namely professional authors weren't discriminated from nonprofessional authors. The border line between them was not clear. Till now, many people and students passed over the problem. So they were confused. Most of socialist activists were lost in the history of literature. That led many researchers of Korean poetry to think that the characteristics of early 1920s' literature is imitation of European literary thoughts. That is not true but only partially truth. An examination of contributors to the above journals reveals many names that are no longer familiar to scholars of Korean poetry. Indeed, they are mainly nonprofessional writers who otherwise political thinkers or socialist activists. An elucidation of the existence of these contributors, as such, can help to shed light on the various ideas that influenced Korean Poetry in the early modern era. Therefore it is necessary to examine the internal relation of literary movement and social activity in early 1920's.

근대 초기 시의 미적 개념 인식과 근대시 장르의 체계화 과정 연구

조영복 우리말글학회 2003 우리말 글 Vol.29 No.-

This thesis is to examine the formation process of the early modern poetry from three different points of view; formal, aesthetic and ideology-historical points. The early modern poetry which has been understood as an imitation of symbolist poems needs to be reviewed from following various angles. First, this thesis proved the fact that the character of coterie of those days was problematic because associates of literacy coterie magazines, such as the Samkwang, the Pyehur, the Jangmichon and Shinsaenghoal etc, are not only literary men but also ideologists. Secondly, the participation of ideologists is related to the formation of early modern poetry's themes, which cannot be easily distinguished from naturalism or mysticism showed in socialist ideology and symbolist poems. Thirdly, this thesis examines concretely the case of Hwang Seok-woo and Namgung Byuk who are estimated as typical symbolist poets. Hwang's poems show an interim step in the way of formal experiment and aesthetic purification of Korean modern poetry. And the issue of self-awakening in Namgung's works can be estimated as a level preparing the formation of modern aestetics, showing the birth of independent human being as modern individual. It is also emphasized in this thesis that the early 1920' poems tend to show excessive romanticism and idealism by stressing so-called 'grandiose self'. This means that it is difficult to regard the romanticism or symbolism of those days as a product of decadent mind which imitated western poetic style. In conclusion, this thesis tries to analyze concretely the forming process of Korean modern poetry from aesthetic, thematic and formal points of view, overcoming the existing attitude of understanding the early modern poems as only one singular discourse of symbolism.

組織理論의 觀點과 모델에 관한 硏究

曺永福 인제대학교 1985 仁濟論叢 Vol.1 No.2

Despite a great need for a general theory of organization that would embrace in a harmonious whole contemporary findings of various disciplines concerned with organizational processes and states everywhere in nature and society, such a theory does not yet exist. It can be argued that our field has witnessed three historical pleases, that is, a period of grand theory, a period of serch for empirical generalization, and a period of development of limited theory. Organizational theorists place different emphases on various aspects of the organization. Perspectives are differentiated on the basis of which aspects of organization they consider to be paramount. It is the interplay between different perspectives that helps one gain a more comprehensive understanding of organizational life, since any one school of thought invariably offers a partial account of seality. In this study, two general types of organizational perspective - functional and conflict, are analyzed to compare their basic assmptions. And not only a dialetical approach to the study of organization but a comparative model of organization as interpretation system is appreciated. The diverse schools of organizational thought are classified according to macro and macro levels of organizational analysis and deterministic versus voluntaristic assumptions of human nature to yield four basic perspectives : system-structural, strategic choice, natural selection, and collective-action views of organizations. In order to formalate and develope organization theory, it is needed to study dialetical perspective of organization and to develope more comprehensive mathematical apparatus of organization felt by Bogdanov at the turn of the century.

서정적 시와 역동적 서정 : 고은 초기 시의 서정성 연구

조영복 광운대학교 인문사회과학연구소 2002 인문사회과학논문집 Vol.31 No.-

This thesis deals with the relation between lyricism and reality in the Ko-Eun'(고은)s early works. His poems, especially 1980s' poems such as 백두산, 만인보 etc, have been generally regarded as poems of realism or poems of actuallism. Then these poems are said to be locking in lyricism and long on reality. According to this point of view, the lyricism is opposed to realism. But that is not always true. Ko-Eun's 1970s' or 1980s' poems are very actual, but on the other hand they connote lyricism. In his poems realism is closely connected with lyricism. The origin can be found in his early poems. That is to say, dynamic lyricism of his poems turned into realism in 1970∼1980s. This thesis has got at the root of this problem throughout Ko-Eun's early poems.