식물병원균 Bipolaris sorokiniana로부터 제초활성 물질의 분리 및 구조결정

임치환 충남대학교 농업과학연구소 2002 농업과학연구 Vol.29 No.2

식물병원균 Bipolaris sorokiniana 배양 추출물로부터 이탈리안 라이그라스 및 논피의 유근신장을 억제하는 활성물질을 분리·정제하여 각종 기기분석을 통하여 화학구조를 결정하였다. 이들 화합물들은 분자식이 C_(8)H_(8)O₃인 benzenoid 화합물인 3-methoxybenzoic acid와 3-hydroxy benzoic acid methyl ester로 숙주식물의 유근신장을 1000ppm에서 90% 이상 저해하였다. 이들 화합물은 식물체의 잎과 줄기에서 분리되어 항균활성 및 항류마티스활성이 있음이 보고된바 있으나 B. sorokiniana로부터 분리되어 제초활성에 관하여 보고하기는 본 논문이 처음이다. Two phytotoxic compounds, 3-methoxybenzoic acid (MBA) and 3-hydroxy benzoic acid methyl ester (HBAME), were purified and structurally characterized by instrumental analyses from a culture of a phytopathogenic fungus, Bipolaris sorokiniana. During the isolation procedure, the toxic components were monitored by the assay using rice (Echinochloa crusgalli) and Italian ryegrass(Lolium multiflorum). These compounds had a molecular formula of C_(8)H_(8)O₃ and inhibited the root growth of the plant seedlings over 90% at a level of 1000 ppm. This is the first report of herbicidal activity of MBA and HBAME purified from B. sorokiniana.

民國初 四川地域 軍閥과 四川鹽政

林地煥 전북대사학회 1990 전북사학 Vol.13 No.-

고구마 검은썩음병균(Lasiodiplodia theobromae)으로부터 식물독소의 분리 및 구조

임치환 충남대학교 농업과학연구소 1998 농업과학연구 Vol.25 No.1

Lasiodiplodia theobromae is a pathogen of Java black rot on sweet potato. This fungus infects the tuberous root during storage after harvest. Invasion of the fungus results in the expansion of necrosis sites into the tuberous roots. The resultant necrotic symptom of the tissue is also induced by application of acetone extract of the fungus growing on potato sucrose agar (PSA) culture. The active principles to induce the necrosis are purified from the acetone extract as follows. After evaporation of hexane-benzene-EtOAc (1:2:1, v/v/v) the extract was fractioned on silica gel column, using a solvent gradient system from n-hexane to EtOAc and then to MeOH. The active fractions were purified with HPLC on Nucleosil 50-5 column by eluting n-hexane to EtOAc. Their structures are established by using spectroscopic techniques and synthesis to 4-hydroxymellein and mellein, respectively. Application of small amount of these compounds induce expansion of the necrotic symptom into the tissue and accumulated ipomeamarone. Conclusively, these compounds acted as phytotoxins (inducing necrosis) and as elicitors (eliciting the phytoalexin).

淸末 民國初 天津錢庄의 發展

林地煥 전북대사학회 1997 전북사학 Vol.19ㆍ20 No.-

토끼에서 Etofenprox와 Spinosad 합제의 국소자극성 시험

황윤환,김명석,송인배,박병권,양승호,임용현,송치용,안성한,남현수,임정교,정상일,명노일,윤효인 忠南大學校 獸醫科大學 附設 動物醫科學硏究所 2008 動物醫科學硏究誌 Vol.15 No.1

This study was performed to evaluation the skin and eye irritation of etofenprox 8% and spinosad 4% combination in male New Zealand White rabbits. In skin irritation test, the combination of etofenprox and spinosad did not showed any change of body weight but caused slight skin irritation with the P.I.I. (primary irritation index) value 0.25. The etofenprox and spinosad combination showed severe eye irritation 1 h, 24 h, 48 h, and 72 h after ocular treatment, having the A.O.I. (acute ocular irritation) value of 37.3. Therefore, these result indicated that the combination of etofenprox and spinosad might be irritant formulation on eye and skin.

야관문(Lespedezea cuneata G. Don) 추출물이 토끼 음경해면체 평활근에 미치는 생리활성

정영호,임치환 충남대학교 농업과학연구소 2005 농업과학연구 Vol.32 No.1

The extracts in n-hexane layer from Lespedezea cuneata G. Don exelled at sexual activity according to in vitro experiment using biopsy of rabbit corpus cavernosum to investigate effects on sexual function. The extracts were injected into 9 samples at 10㎕. The lowest relaxation was showed at sample C, 4.8±1.4% and the highest relaxation was showed at sample A, 20.2±6.0%. The relaxation by every sample, except sample C and G-1, were higher than by 10^(-7)M ACh, 7.8±5.1%, and their effects were above 10%. Also, the extracts were injected into 9 samples at 15㎕. The lowest relaxation was showed at sample C, 15.4±1.3% and the highest relaxation was showed at sample J, 54.8±9.7%. The relaxationby sample A and D was as much as by 10^(-6)M ACh, 28.0±20.1%. The relaxation by sample H was 41.9±7.3%. The relaxation by sample J was 54.8±9.7% and it was higher than by 10^(-5)M ACh, 53.9±25.9%. Also, the extracts were injected into 9 samples at 20㎕. The lowest relaxation was showed at sample E, 28.9±0.6% and it was a little higher than by 10^(-6)M ACh. The relaxation by sample G was as much as by 10^(-5)M ACh. The two higher relaxation were showed at sample H, 99.4±16.0% and J, 95.7±7.2%, and their relaxation against contraction reaction by PhE were near 100%. Experiment by sample I was not performed for lack of sample amount.

산채류로부터 혈소판응집 억제물질의 검색

윤민호,임치환,오진환,이종철,최우영 충남대학교 농업과학연구소 1997 농업과학연구 Vol.24 No.2

To select potential inhibitors of platelet aggregation form large numbers of crude plant extracts, the modified thin smear method for the anti-platelet aggregating activity using platelet rich plasma was further modified by direct observation under a light microscope without staining the smear. The activities determined by the method were coincided with those by the electrical impedence method using whole blood, when ADP or collagen was employed as the aggregating agent. Among 130 varieties of edible and herbal plants which collected from markets or experimental farms of agricultural research institutes, those showed the anti-platelet aggregating activities were selected by testing the crude methanol extracts: Aster scaber, Aster tataricus, Ligularia stenocephala, Platycodon glaucum Allium victorials, Allium oderum, Morus bombycis, Portulaca oleracea, Acanthopanax sessiliflorus and Rosa davurica. However, some of them activated the platelet aggregation under the same assay conditions: Pimpinella brachycarpa, Hosta plantaginea. Capsella bursapastoris, Fagopym esculentum, Prunus mume, Rubus coreanus and Limaria japonica. In addition, those revealed the antioxidant activities were selected by measuring the abilities to scavenge superoxide anion radicals: Pteridum aquilinum, Aster scaber, Ligularia fischeri, Chrysanthemum zawadskii, Artemisia capipparis, Cirsium setidens, Commelina communis and Capsella bursapastoris among edible plants.

새로운 抗菌劑로서 1-(phenoxymethyl)benzotriazole 誘導體의 合成과 定量的 構造活性關係(QSAR) 分析

成洛道,林治煥,崔宇永,高東成,權奇星 충남대학교부설 생명공학연구소 1992 생물공학연구지 Vol.2 No.-

14종의 새로 합성된 1-(phenoxymethyl)benzotriazole(Ⅰ)(Y=0)과 1-(thiophenoxymethyl)benzotriazole (Ⅱ)(Y=S) 및 1-(azidomethy) benzotriazole(Ⅲ) 유도체의 구조와 in vitro에서 Pyricularia oryzae, Fusarium oxysporum f. sp. sesami, Valsa ceratosperma 및 Botrytis cinerea에 대한 균사 생장을 50% 저해하는 활성(pI_50)사이의 구조-항균활성 상관관계들을 QSAR방법으로 연구하였다. (Ⅰ)의 항균활성은 (Ⅱ)와 (Ⅲ)보다 우세하였으며 phenoxy group(Ⅰ)의 치환기 효과는 수소 결합성 (HB)과 포물선 관계의 electronic effect(σ), steric effect(B_1) 그리고 hydrophobic effect(π)로 설명된다. P. oryzae와 F. axysporum f. sp. sesami의 항균 활성에 대한 치환기의 적정값은 B_1=1.40A, (H)와 σ=0.07∼0.15, (H)이고 V. ceratosperma와 B. cinerea에 대하여는 각각 σ=0.23∼0.28, (C1)과 π=0.70, (C1)이었으며 가장 효과적인 화합물인 (Ia)와 (Id)의 구조-활성관계가 검토되었다 (1990년 5월 11일 접수, 1990년 9월 20일 수리). The structure-antifungal activity correlations between the structure of fourteen new 1-(phenoxymethyl)benzotriazoles (Ⅰ) (Y=0), 1-(thiophenoxymethyl)benzotriazoles (Ⅱ) (Y=S) and 1-(azidomethy)benzotriazole (Ⅲ) derivatives were synthesized, and their activity, fifty percent inhibition of mycelial growth(pI_50), in vitro against Pyricularia oryzae, Fusarium oxysporum f. sp sesami, Valsa ceratosperma and Botrytis cinerea were investigated using a generalized QSAR method. The activity of (Ⅰ) was superior to those of (Ⅱ) and (Ⅲ). The effect of the substituents (Ⅹ) on the phenoxy group (Ⅰ) was rationalized by a parabolic function of electronic (σ), steric (B_1) and hydrophobic parameter(π), and hydrogen bonding (HB). Where the optimal values of substituent on the fungicidal activity againt P. oryzae and F. axysporum f. sp. sesami are B_1=1.40A ; (H) and σ=0.07∼0.15;(H), and those of substituent on the fungicidal activity against V. ceratosperma and B. cinerea are σ=0.23∼0.28; (Cl), π=0.70; (C1), respectively. The most effective compound (Ⅰa) and (Ⅰd) were examined in this study.

수압보증시험시의 음향방출에 의한 복합재 연소관의 파괴거동 예측

이상호,김호철,오치환,송성진,정현조,임수용 韓國非破壞檢査學會 1998 한국비파괴검사학회지 Vol.18 No.2

섬유강화 복합제 연고관은 복합재료의 사용, 필라멘트 와인딩 및 경화공정 등으로 인하여 제작 중 다양한 결함이 발생할 수 있으며, 또한 사용 도중에도 예기치 않은 손상을 받아 기계적 성질이 저하될 수 있다. 따라서 제작 완료된 연소관의 품질 보증을 위해 수압보증시험이 사용되고 있으나 이를 위해 가한 수압이 오히려 연소관에 예기치 않은 손상을 가하여 실제 사용시 그 성능을 저하시키는 요인으로 작용하는 경우도 있다. 이러한 문제를 해결하기 위해 본 연구에서는 수압보증시험시의 음향방축을 감지하여, 그 신호의 특성으로부터 복합제 연소관의 파손 거동을 예측하는 체계적인 기법의 개발을 시도하였다. 음향방출을 이용하여 연소관에 발생한 손상의 개략적 위치를 결정할 수 있었으며, 또한 파괴모드의 식별도 가능하였다. 다만, 시료의 부족으로 최종파열압력의 변화와 충격손상의 영향에 대한 신뢰성있는 분석은 불가능하였다. Prediction of failure behavior in filament-wound composite motor cases is one of the important issues for their reliable application. Acoustic emission during hydroproof testing of the cases is used to solve this problem. Based on the acoustic emission behavior, failure sites can be located successfully. The identification of failure modes is also possible using the distribution of acoustic emission amplitude. Due to the limitation in the number of samples, it is not possible to predict the final burst pressure of motor cases and the effect of impact damage on the final burst pressure.

피틴산(Phytic acid)이 비브리오(Vibrio vulnificus) 패혈증에 미치는 영향

정영호,조천휘,이선우,임치환 충남대학교 농업과학연구소 2005 농업과학연구 Vol.32 No.1

Phytic acid chelates excellently the metallic ions and the positive ions, especially has high affinity with Fe^(2+) and Ca^(2+). Merits of phytic acid can be taked in easily, edibile and harmless to body, so it was investigated that phytic acid can be substituted for EDTA in this study. 1. The Intensificative effect of chelating agent and disinfective osmotic shock of Vibrio vulnificus The number of initial existent fungi measured 1.7x10^(6). The percentages of the survival fungi against the osmotic shock by distillated water were calculated at 1 minute, 3 minute and 5 minute after inoculation. The percentages of the survival fungi in Mg^(2+) were 92.5%, 91.8% and 79.8% at each time, the average percentage was 88%. Also the sudden extinction was observed around 1 minute after inoculation and the survival fungi were not observed from 3 through 5 minute in spite of repeated experimentation. 2. Influence of Vibrio vulnificus on the survival of the mice. The first mouse started to die in 180 minute after inoculation in case that the inoculating number was 2.3x10^(7) cfu/ml. All died within 4.5 hour. The average of survival time was 226 minute. The first mouse started to die in 228 minute after inoculation in case that the inoculating number was 0.8x10^(6) cfu/ml. All died within 5 hour. The average of survival time was 300 minute and the survival time was 1.3 times high. The tendencies of death in two cases were similar, but the fatal rate were largely dependent on inoculating number.