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Modification of a Smoking Motivation Questionnaire for Chinese Medical Students
Jiang, Chao,Sun, Wen-Jie,Wan, Yan-Chun,Wei, Ming-Wei,Mu, Yong-Ping,Tarver, Siobhan L.,Gao, Yong-Qing,Hu, Tian,Xu, Chao,Gordon, James,Feng, Cindy Xin,Wen, Yu-Feng Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.6
Introduction: Smoking prevalence among the medical students is high in China. Therefore, understanding the smoking motivations of medical students is crucial for smoking control, but currently there are no scales questionnaires customized for probing the smoking motivations of medical students. This aim of study was to test and modify a questionnaire for investigating smoking motivations among medical students. Methods: A cross-sectional survey was conducted among 1,125 medical students at Xuzhou Medical College in China in 2012.The model fit and validity was assessed by confirmatory factor analysis (CFA) and the reliability was tested by single-item reliability, composite reliability, and item-total correlation. Results: The prevalence of smoking was 9.84 % among study population. In the modified scales, the global fit indices identified a CFI value of 0.96, TLI was 0.96, and the RMSEA was 0.063. CFA supported the two dimensional structure of the instrument. The average variance extracted ranged from 0.45 to 0.62. All single-item reliability scores were greater than 0.20, and the composite reliability ranged from 0.74 to 0.91. Conclusion: Modified scales could be the preliminary instrument used in evaluating the smoking motivations of medical students. However, it should be further assessed using other forms and methods of validity and reliability, additional motivations of smoking, and the survey of other medical colleges in China.
Chao Chen,Hua-Feng Li,Yu-Jie Hu,Meng-Jie Jiang,Qing-Sheng Liu,Jia Zhou 연세대학교의과대학 2019 Yonsei medical journal Vol.60 No.7
Purpose: Family with sequence similarity 83 member H (FAM83H) plays key roles in tumorigenesis. However, the specific rolesof FAM83H in cervical cancer (CC) have not been well studied. Materials and Methods: The RNA-seq data of 306 CC tissues and three normal samples downloaded from The Cancer GenomeAtlas were used to analyze the expression of FAM83H. The Kaplan-Meier method was used to draw survival curves. Associationsbetween FAM83H expression and clinicopathological factors were analyzed by chi-square test. Cox proportional hazards modelwas used to analyze prognostic factors. Loss-of-function assays were conducted to discover the biological functions of FAM83Hin cell proliferation, colony formation, invasion, and migration. Real-time Quantitative Reverse Transcription PCR (qRT-PCR)and Western blotting were used to measure the expression levels of FAM83H in CC cell lines. Results: Our results demonstrated that FAM83H is overexpressed in CC tissues and that high FAM83H expression is associatedwith worse overall survival (OS). High FAM83H expression in CC was associated with clinical stage, pathologic tumor, and pathologicnode. Univariate analysis suggested that FAM83H expression was significantly related to the OS of CC patients. Althoughmultivariate analysis showed that FAM83H expression was not an independent prognostic factor for the OS of CC patients, the effectsof FAM83H on CC cell growth and motility was significant. Loss-of-function experiments demonstrated that knockdown ofFAM83H inhibited proliferation, colony formation, migration, and invasion of CC cells by inactivating PI3K/AKT pathway. Conclusion: FAM83H might play a crucial role in CC progression and could act as a novel therapeutic target in CC.
( Chao Wei Huang ),( Ko Chiao Yen ),( Chao Feng Yu ),( Ying Chen Lu ) 한국농업기계학회 2018 한국농업기계학회 학술발표논문집 Vol.23 No.1
Many Gram-positive bacteria, particularly lactic acid bacteria, are known to secrete proteins that have antimicrobial activity. These proteins, known as bacteriocins, have been shown to display inhibitory activity against many pathogens. Use lactic acid bacteria fermented herb has many advantages, such as increase the extraction rate of herb, produce new active compounds, reduce waste etc. In this study, 270 strains of lactic acid bacteria were screened for bacteriocin producing ability, and Lactobacillus gasseri LYC400 was found to have highest bacteriocin activity. Lactobacillus gasseri LYC400 was used to ferment MRS broth with various concentration, 0, 2, 4, 6%, respectively, wild bitter melon (Momordica charantia Linn. var. abbreviata Ser.) powder. The fermentation broths were centrifuged and the supernatants were collected follow by protein extraction to analyze bacteriocin activity. The results showed wild bitter melon powder was able to support good growth of the lactic acid bacterium. The bacterial number, lactic acid concentration and the E. coli inhibition ability of protein extractions were positive correlated with bitter melon powder concentration. However, the Staphylococcus aureus inhibition ability of protein extractions were increased with 2, 4% bitter melon powder but decreased at 6% bitter melon powder. Fermentation wild bitter melon via Lactobacillus gasseri LYC400 increases its antibacterial activity with putative anti-diabetic activities may have great potential for health food.
Kuo-Feng Hua,A-Ching Chao,Ting-Yu Lin,Wan-Tze Chen,Yu-Chieh Lee,Wan-Han Hsu,Sheau-Long Lee,Hsin-Min Wang,Ding-I. Yang,Tz-Chuen Ju 고려인삼학회 2022 Journal of Ginseng Research Vol.46 No.4
Background: Huntington's disease (HD) is a neurodegenerative disorder caused by the expansion oftrinucleotide CAG repeat in the Huntingtin (Htt) gene. The major pathogenic pathways underlying HDinvolve the impairment of cellular energy homeostasis and DNA damage in the brain. The protein kinaseataxia-telangiectasia mutated (ATM) is an important regulator of the DNA damage response. ATM isinvolved in the phosphorylation of AMP-activated protein kinase (AMPK), suggesting that AMPK plays acritical role in response to DNA damage. Herein, we demonstrated that expression of polyQ-expandedmutant Htt (mHtt) enhanced the phosphorylation of ATM. Ginsenoside is the main and most effectivecomponent of Panax ginseng. However, the protective effect of a ginsenoside (compound K, CK) in HDremains unclear and warrants further investigation. Methods: This study used the R6/2 transgenic mouse model of HD and performed behavioral tests,survival rate, histological analyses, and immunoblot assays. Results: The systematic administration of CK into R6/2 mice suppressed the activation of ATM/AMPK andreduced neuronal toxicity and mHTT aggregation. Most importantly, CK increased neuronal density andlifespan and improved motor dysfunction in R6/2 mice. Conversely, CK enhanced the expression of Bcl2protected striatal cells from the toxicity induced by the overactivation of mHtt and AMPK. Conclusions: Thus, the oral administration of CK reduced the disease progression and markedlyenhanced lifespan in the transgenic mouse model (R6/2) of HD.
MUTYH Association with Esophageal Adenocarcinoma in a Han Chinese Population
Kong, Feng,Han, Xue-Ying,Luan, Yun,Qi, Tong-Gang,Sun, Chao,Wang, Jue,Hou, Hua-Ying,Jiang, Yu-Hua,Zhao, Jing-Jie,Cheng, Guang-Hui Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.11
Adenocarcinoma of esophagus (AE) is a complex disease, affected by a variety of genetic and environmental factors. Much evidence has shown that the MutY glycosylase homologue (MUTYH) plays a key role in the pathogenesis of many cancers. However, there have been no reports on influence on AE in the Han Chinese population. The objective of this study was to investigate this issue. A gene-based association study was conducted using three single nucleotide polymorphisms(SNPs) reported in previous studies. The three SNPs (rs3219463, rs3219472, rs3219489) were genotyped in 207 unrelated AE patients and 249 healthy controls in a case-control study using polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP). The results revealed that the genotype distribution of rs3219472 differed between the case and control groups (OR=1.66,95%CI=1.11-2.48, P=0.012), indicating that an association may exist between MUTYH and AE. These findings support a signifcant role for MUTYH in AE pathogenesis in the Han Chinese population.
Ji-Feng Shi,Qing-Yu Xu,Wen-Chao Guo,Guo-Qing Li 한국응용곤충학회 2016 Journal of Asia-Pacific Entomology Vol.19 No.3
Trehalosemetabolism is critical for production of ATP, provision of carbon source, and facilitation of carbohydrate absorption. Trehalose-6-phosphate synthase (TPS) is a rate-limiting enzyme in trehalose biosynthesis. In the present paper, a TPS gene (LdTPS)was cloned inLeptinotarsa decemlineata. At young larval instars, the expression levels of LdTPS were high just before and right after ecdysis, and were low at the mid instar stages. In the fourthinstar larvae, two peaks occurred at 24 h after ecdysis and at the wandering stage. In vitro midgut culture and an in vivo bioassay revealed that 20E stimulated LdTPS transcription. Conversely, a reduction of 20E by RNA interference (RNAi) of a prothoracicotropic hormone receptor gene LdTorso and an ecdysteroidogenesis gene LdSHD repressed LdTPS expression. Moreover, disruption of 20E signaling by knockdown of LdEcR, LdE75 and LdFTZ-F1 reduced LdTPS transcript levels. Similarly, in vitro culture and an in vivo bioassay showed that exogenous JH or JH analog methoprene and pyriproxyfen activated LdTPS expression. An increase in endogenous JH by RNAi of an allatostatin gene LdAS-C enhanced the transcription. In contrast, a decrease in JH by silencing of a JH biosynthesis gene LdJHAMT downregulated LdTPS expression. Moreover, knockdown of LdILP2 repressed LdTPS transcription. Therefore, LdTPS transcription is tuned by 20E, JH and ILP signaling pathways in L. decemlineata.
Chun-Yu Liu,Tzu-Ting Huang,Pei-Yi Chu,Chun-Teng Huang,Chia-Han Lee,Wan-Lun Wang,Ka-Yi Lau,Wen-Chun Tsai,Tzu-I Chao,Jung-Chen Su,Ming-Huang Chen,Chung-Wai Shiau,Ling-Ming Tseng,Kuen-Feng Chen 생화학분자생물학회 2017 Experimental and molecular medicine Vol.49 No.-
Triple-negative breast cancer (TNBC) remains difficult to treat and urgently needs new therapeutic options. Nintedanib, a multikinase inhibitor, has exhibited efficacy in early clinical trials for HER2-negative breast cancer. In this study, we examined a new molecular mechanism of nintedanib in TNBC. The results demonstrated that nintedanib enhanced TNBC cell apoptosis, which was accompanied by a reduction of p-STAT3 and its downstream proteins. STAT3 overexpression suppressed nintedanib-mediated apoptosis and further increased the activity of purified SHP-1 protein. Moreover, treatment with either a specific inhibitor of SHP-1 or SHP-1-targeted siRNA reduced the apoptotic effects of nintedanib, which validates the role of SHP-1 in nintedanib-mediated apoptosis. Furthermore, nintedanib-induced apoptosis was attenuated in TNBC cells expressing SHP-1 mutants with constantly open conformations, suggesting that the autoinhibitory mechanism of SHP-1 attenuated the effects of nintedanib. Importantly, nintedanib significantly inhibited tumor growth via the SHP-1/p-STAT3 pathway. Clinically, SHP-1 levels were downregulated, whereas p-STAT3 was upregulated in tumor tissues, and SHP-1 transcripts were associated with improved disease-free survival in TNBC patients. Our findings revealed that nintedanib induces TNBC apoptosis by acting as a SHP-1 agonist, suggesting that targeting STAT3 by enhancing SHP-1 expression could be a viable therapeutic strategy against TNBC.