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Research on the Characteristic of Diesel Elastic-plate Impingement Spray
( Chang Yuan Wang ),( Yong Shang ),( Xiang Rong Li ),( Fu Shui Liu ),( Cheng Hui Yu ) 한국액체미립화학회 2010 한국액체미립화학회 학술강연회 논문집 Vol.2010 No.-
A series of experimental research results on the characteristic of diesel elastic-plate impingement spray using High Speed Photography camera are presented in this paper. The experiments were carried out in a constant volume chamber specially designed, which can hold a high ambiance pressure. The special fixed device was designed so that the elastic-plate can be fixed on the spray path, meanwhile the spray incident angle and height can be changed. The free jet spray and elastic-plate impingement spray was compared under the same experimental condition including different injection pressure and different background pressure. Experimental research showed that impingement spray droplets diffuse more quickly than free jet spray.
RO 및 NF막에서의 부식질에 의한 Fouling 및 미량오염물질의 제거
왕창근,차준철,이용현,김수동,류주환 대한상하수도학회 2004 상하수도학회지 Vol.18 No.1
This study investigated the phenomena of membrane fouling by NOM and the effect of the fouling on removal of micro-pollutants. NOM has a great effect on decline of permeate flux. Permeate flow rate was reduced by 88% in RO and 34.8% in NF for 323hr operation period. Removal rate of UV_(254), is 87.4% in RO and 78.5% in NF and removal rate of DOC is 42.7% in RO and 32.9% in NF for 2㎎/l humic acid. Removal efficiency of the micmpollutants by the RO and NF membranes fouled by humic acid was mostly lower than that by the new membrane. The concentration polarization which affects the flux and the rejection was thought to occur in the active layer of the membrane, as the membrane was getting fouled.
Liu, Yi-Qing,Zhang, Guo-An,Zhang, Bing-Chang,Wang, Yong,Liu, Zheng,Jiao, Yu-Lian,Liu, Ning,Zhao, Yue-Ran Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.3
Background: Prostate cancer is one of the main causes of cancer death, and drug resistance is the leading reason for therapy failure. However, how this occurs is largely unknown. We therrfore aimed to study the response of DU145 cells to cisplatin. Materials and Methods: Du145 prostate cancer cells were treated with a low dose of cisplatin for 24 h and cell viability and number were determined by MTT assay and trypan blue exclusion assay, respectively. The real time polymerase chain reaction (PCR) was used to assess responses to cisplatin treatment. Results: After 24h $2{\mu}g/ml$ treatment did not result in significant reduction in cell viability or number. However, it led to enhanced cancer cell invasiveness. E-cadherin mRNA was reduced, and vimentin, Snail, Slug, metalloproteinase 9 (MMP9) mRNA expression increased significantly, a feature of epithelial-mesenchymal transition (EMT). Conclusions: Short time low concentration cisplatin treatment leads to elevated invasiveness of DU145 cancer cells and this is possibly due to EMT.
Effect of gelatin nano-coating containing Gardenia pigment on the preservation of pork slices
Yong Liu,Zi-Hao Liu,Chang-Qi Luo,Chun-Tao Xiao,Wen-Yu Zhou,Wen-Jin Xie 한국식품과학회 2022 Food Science and Biotechnology Vol.31 No.4
The nano-coating composed of gelatin and Gardenia pigment (GP) was successfully prepared and showed strong antioxidant activity. The average particle sizes of the nano-coating containing 0.1% and 0.3% GP were 269.58 and 394.13 nm, respectively. The pork slices uncoated and coated with the nano-coating were preserved at 4 C for 15 days. The pork slices’ pH, total volatile basic nitrogen (TVB-N), total viable counts (TVC), water-bind- ing capacity (WHC), and thiobarbituric acid reactive sub- stances (TBARS) were measured to assess the preservation effect of the nano-coating. The results showed that the pork coated with the nano-coating had lower pH, TVC, TVB-N, TBARS, and higher WHC, significantly different (p \ 0.05) than the uncoated pork. It is suggested that the proposed nano-coating can be used to effectively improve the pork’s quality and shelf life during refrigeration storage.
최창용(Chang Yong Choi),권상구(Sang Gu Kwon),유준(Jay Liu),임도진(Do Jin Im) 한국청정기술학회 2017 청정기술 Vol.23 No.2
본 연구에서는 연료전지용 디젤 흡착 탈황 반응기에 사용된 촉매를 재생하는 공정을 수치해석을 통해 모사하고 분석하여 관련된 기초 공정 정보를 도출하였다. 촉매 재생에 사용되는 질소 퍼지가스의 유량, 충전된 탈황촉매의 투과율, 반응기의 크기, 반응기의 단열 성능에 따른 정상상태 해석을 통해 각각의 요소가 촉매 재생에 미치는 영향들을 살펴보았다. 온도의 영향을 거의 받지 않았던 탈황공정과 달리 촉매의 재생 공정에서는 온도 변화에 따라 영향을 크게 받았으며 이전 탈황 연구에서 중요한 공정 변수였던 충전된 촉매의 투과도, 충전층의 공극률 등은 큰 영향을 미치지 못했다. 비정상상태 해석을 수행하여 재생에 소요되는 시간을 예측하였으며 퍼지가스의 유량과 온도를 변화시키며 재생에 소요되는 시간을 분석한 결과, 퍼지가스의 유량을 높이는 것 보다 온도를 높이는 것이 재생에 더 효율적임을 확인하였다. 본 연구 결과는 선박 연료전지용 디젤의흡착 탈황 반응기의 재생 공정 개발에 활용될 것으로 기대된다. 또한 본 결과는 연료전지뿐 아니라 일반적으로 정유사에서 생산되는 디젤유의 황 함유량을 감소시키는 저황 시스템 디자인에 활용될 수 있으며 이러한 의미에서 석유화학 산업의 청정화 기술 확보에 이바지할 것으로 기대된다. In this study, we performed numerical simulation for the catalyst regeneration process of diesel desulfurization reactor. We analyzed the changes in regeneration process according to purge gas flow rate, catalyst permeability, reactor size, and heat loss of reactor. We have found that the regeneration process is very much affected by temperature changes whereas it is hardly affected by catalyst permeability and porosity. We also estimated the regeneration time according to purge gas flow rate and initial temperatures and have found that increasing purge gas temperature is more effect for fast regeneration. The present results can be utilized to design a regeneration process of diesel desulfurization reactor for a fuel cell used in ships. Furthermore, the present work also can be used to design low sulfur diesel supply in oil refineries and therefore contribute to the development of clean petrochemical technology.
( Tian Zhou Liu ),( Bang Xing Wang ),( Jin Tao Guo ),( Yang Zhou ),( Mugweru Julius ),( Moses Njire ),( Yuan Yuan Cao ),( Tian Wu ),( Zhi Yong Liu ),( Chang Wei Wang ),( Yong Xu ),( Tian Yu Zhang ) 한국미생물 · 생명공학회 2015 Journal of microbiology and biotechnology Vol.25 No.9
The combination of trimethoprim (TMP) and sulfamethoxazole (SMX) has been shown to be active against Mycobacterium tuberculosis (Mtb) in clinical tuberculosis (TB) treatment. However, the mechanism of action of TMP-SMX against Mtb is still unknown. To unravel this, we have studied the effect of TMP and SMX by deleting the folP2 gene in Mycobacterium smegmatis (Msm), and overexpressing the Mtb and Msm folP1/2 genes in Msm. Knocking out of the folP2 gene in Msm reduced the minimum inhibitory concentration of SMX 8-fold compared with wild type. Overexpression of the folP1 genes from Mtb and Msm increased the MICs by 4- and 2-fold in Msm for SMX and TMP, respectively. We show a strong correlation between the expression of folP1 and folP2 genes and TMP-SMX resistance in mycobacteria. This suggests that a combination of FolP2 inhibitor and SMX could be used for TB treatment with a better outcome.
Metabolic Engineering of Probiotic <i>Saccharomyces boulardii</i>
Liu, Jing-Jing,Kong, In Iok,Zhang, Guo-Chang,Jayakody, Lahiru N.,Kim, Heejin,Xia, Peng-Fei,Kwak, Suryang,Sung, Bong Hyun,Sohn, Jung-Hoon,Walukiewicz, Hanna E.,Rao, Christopher V.,Jin, Yong-Su American Society for Microbiology 2016 Applied and environmental microbiology Vol.82 No.8
<P>Saccharomyces boulardii is a probiotic yeast that has been used for promoting gut health as well as preventing diarrheal diseases. This yeast not only exhibits beneficial phenotypes for gut health but also can stay longer in the gut than Saccharomyces cerevisiae. Therefore, S. boulardii is an attractive host for metabolic engineering to produce biomolecules of interest in the gut. However, the lack of auxotrophic strains with defined genetic backgrounds has hampered the use of this strain for metabolic engineering. Here, we report the development of well-defined auxotrophic mutants (leu2, ura3, his3, and trp1) through clustered regularly interspaced short palindromic repeat (CRISPR)-Cas9-based genome editing. The resulting auxotrophic mutants can be used as a host for introducing various genetic perturbations, such as overexpression or deletion of a target gene, using existing genetic tools for S. cerevisiae. We demonstrated the overexpression of a heterologous gene (lacZ), the correct localization of a target protein (red fluorescent protein) into mitochondria by using a protein localization signal, and the introduction of a heterologous metabolic pathway (xylose-assimilating pathway) in the genome of S. boulardii. We further demonstrated that human lysozyme, which is beneficial for human gut health, could be secreted by S. boulardii. Our results suggest that more sophisticated genetic perturbations to improve S. boulardii can be performed without using a drug resistance marker, which is a prerequisite for in vivo applications using engineered S. boulardii.</P>
Actinorugispora endophytica gen. nov., sp. nov., an actinomycete isolated from Daucus carota.
Liu, Min-Jiao,Zhu, Wen-Yong,Li, Jie,Zhao, Guo-Zhen,Xiong, Zhi,Park, Dong-Jin,Hozzein, Wael N,Kim, Chang-Jin,Li, Wen-Jun Society for General Microbiology 2015 International journal of systematic and evolutiona Vol.65 No.8
<P>An actinomycete strain, designated YIM 690008T, was isolated from Daucus carota collected from South Korea and its taxonomic position was investigated by using a polyphasic approach. The strain grew well on most media tested and no diffusible pigment was produced. The aerial mycelium formed wrinkled single spores and short spore chains, some of which were branched. The whole-cell hydrolysates contained meso-diaminopimelic acid, glucose, mannose, ribose, galactose and rhamnose. The predominant menaquinones were MK-10(H4), MK-10(H6), MK-10(H8) and MK-10(H2). The polar lipids were diphosphatidylglycerol, phosphatidylcholine, phosphatidylinositol, phosphatidylinositol mannosides, some unknown phospholipids, glycolipids and polar lipids. The major fatty acids were i-C16?:?0, ai-C17?:?0 and C18?:?1ω9c. The DNA G+C content of the genomic DNA was 63.1?mol%. Phylogenetic analysis indicated that the isolate belongs to the family Nocardiopsaceae. However, based on phenotypic, chemotaxonomic and genotypic data, it was concluded that strain YIM 690008T represents a novel genus and novel species of the family Nocardiopsaceae, for which the name Actinorugispora endophytica gen. nov., sp. nov. (type strain YIM 690008T?=?DSM 46770T?=?JCM 30099T?=?KCTC 29480T) is proposed.</P>
Liu, Ya-Qi,Park, Nam Sook,Kim, Yong Gyun,Kim, Keun Ki,Park, Hyun Chul,Son, Hong Joo,Hong, Chang Ho,Lee, Sang Mong Korean Society of Sericultural Science 2014 International Journal of Industrial Entomology Vol.28 No.2
The full-length heat shock protein 88 (HSP88) complementary DNA (cDNA) of Paecilomyces tenuipes Jocheon-1 was obtained by screening the Paecilomyces tenuipes (P. tenuipes) Jocheon-1 Uni-Zap cDNA library and performing 5' RACE polymerase chain reaction (PCR). The P. tenuipes Jocheon-1 HSP88 cDNA contained an open reading frame (ORF) of 2,139-basepair encoding 713 amino acid residues. The deduced amino acid sequence of the P. tenuipe s Jocheon-1 HSP88 cDNA showed 77% identity to Nectria haematococca HSP88 and 45-76% identity to other fungal homologous HSP88s. Phylogenetic analysis and BLAST program analysis confirmed that the deduced amino acid sequences of the P. tenuipes Jocheon-1 HSP88 gene belonged to the ascomycetes group within the fungal clade. The P. tenuipes Jocheon-1 HSP88 also contained the conserved ATPase domain at the N-terminal region. The cDNA encoding P. tenuipes Jocheon-1 HSP88 was expressed as an 88 kilodalton (kDa) polypeptide in baculovirus-infected insect Sf9 cells. Under higher temperature conditions for the growth of the entomopathogenic fungus, mRNA expression of P. tenuipes Jocheon-1 HSP88 was quantified by real time PCR (qPCR). The results showed that heat shock stress induced a higher level of mRNA expression compared to normal growth conditions.