Effect of intense pulsed light on the deactivation of lipase: Enzyme-deactivation kinetics and tertiary structural changes by fragmentation

Jeon, Min-Sik,Park, Kyung-Min,Yu, Hyunjong,Park, Jun-Young,Chang, Pahn-Shick Elsevier 2019 Enzyme and microbial technology Vol.124 No.-

<P><B>Abstract</B></P> <P>The effect of intense pulsed light (IPL) irradiation on <I>Chromobacterium viscosum</I> lipase was investigated with a primary focus on catalytic activity and molecular structure. During IPL irradiation, lipase activity decreased significantly with increasing pulse fluence (F<SUB>p</SUB>) and exposure time (t<SUB>e</SUB>). IPL-induced deactivation kinetics were further elucidated based on a two-step series-type deactivation model (constant deactivation rate <I>k</I> <SUB>1</SUB> ><I>k</I> <SUB>2</SUB>). F<SUB>p</SUB> was found to be the dominant variable affecting the degree of lipase deactivation, and residual activity was not associated with increasing t<SUB>e</SUB> below a certain F<SUB>p</SUB> energy density (2.66 mJ/cm<SUP>2</SUP>), implying a critical threshold for IPL-induced deactivation of lipase. From the results of fluorescence spectroscopy and sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), we determined that IPL-induced deactivation was caused by fragmentation, leading to lipase tertiary structural changes. Furthermore, the results of FindPept analysis and matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) indicated that the internal sensitive bonds of lipase were cleaved preferentially by IPL, such that IPL irradiation induced site-sensitive fragmentation and peptide bond cleavage.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Intense pulsed light (IPL) irradiation deactivated <I>Chromobacterium viscosum</I> lipase. </LI> <LI> Lipase deactivation was elucidated by the two-step series type deactivation kinetics. </LI> <LI> Pulse fluence (F<SUB>p</SUB>) was the dominant variable affecting the IPL-induced deactivation. </LI> <LI> The lipase deactivation was caused by fragmentation of protein structure. </LI> <LI> IPL irradiation induced site-sensitive fragmentation and peptide bond cleavage. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

Endoscopy and Imaging Modalities/Basic Science of Gastrointestinal Disorders/Miscellaneous : Changing Pattern Of Digestive And Liver Disease In Korea, 1990-2006 Year; A Single Center Study

( Jung Hyun Kwon ),( Sang Woo Kim ),( In Sik Chung ),( Myung Gyu Choi ),( Kwan Woo Nam ),( Jung Pil Suh ),( Jae Hyuck Chang ),( Won Haing Hur ),( Yu Kyung Cho ),( Jae Myung Park ),( In Seok Lee ) 대한소화기학회 2007 SIDDS Vol.9 No.-

Background/Aims: Westernization in many Asian countries have changed lifestyles and diets, so once rare diseases have now become prevalent. The aim of this study is to investigate the changing pattern of digestive and liver disease in Korea, from 1990 to 2006. Methods: We extracted data specific gastrointestina (GI) disease based on the International Classification of Diseases code from the in-patients records at the Kangnam St. Mary`s Hospital in 1990, 1996 and 2006. This hospital is a tertiary-care hospital in Seoul, Korea, which has a capacity of 800 beds. Results: The admission rate for GI disease increased between 1990 and 2006. Overall in-patients were 1,623 persons in 1990, 2,368 persons in 1996 and 4,166 persons in 2006. The mean age of in-patients increased as time went by. A stomach cancer was the most common diagnosis during all periods, but its prevalence has decreased. Colon cancer ranked the 7th in 1990, but markedly increased and now ranks the second. The third was a hepatocellular carcinoma. The bile duct and gallbladder cancer, pancreas and esophageal cancer ranking followed with little interval change. In cases of cancer patients, a regular admission dramatically increased for chemotherapy. The number of patients admitted with pre-malignant neoplasm of stomach and colon increased remarkably with the development of endoscopy. The liver transplant, inflammatory bowel discase, and reflux esophagitis emerged form mid-1990s with greater frequencies, yet much below the levels found in the West. The admission rate for peptic ulcer, especially ulcer bleeding remained relatively stable, despite a decreased rate for ulcer perforation. Liver cirrhosis, hepatitis, cholelithiasis with cholecystitis-cholangitis, appendicitis, hemorrhoid and gastritis all decreased. Conclusions: The stomach cancer is the leading cause of admission, despite a recent decline. Colon cancer showed a marked rise. The liver transplant, inflammatory bowel disease and reflux esophagitis were new diagnosis with an increased tendency.

Effect of epidural corticosteroid injection on magnetic resonance imaging findings

( Min Soo Kim ),( Tae Yoon Jeong ),( Yu Seon Cheong ),( Young Wook Jeon ),( So Young Lim ),( Seong Sik Kang ),( In Nam Kim ),( Tsong Bin Chang ),( Hyun Ho Seong ),( Byeong Mun Hwang ) 대한통증학회 2017 The Korean Journal of Pain Vol.30 No.4

Background: Magnetic resonance imaging (MRI) of the spine is the preferred diagnostic tool for pathologic conditions affecting the spine. However, in patients receiving epidural corticosteroid injection (ESI) for treatment of spinal diseases, there is a possibility of misreading of MR images because of air or fluid in the epidural space after the injection. Therefore, we defined the characteristics of abnormal changes in MRI findings following an ESI in patients with low back pain. Methods: We reviewed the medical records of 133 patients who underwent MRI of the lumbar spine within 7 days after ESI between 2006 and 2015.All patients were administered an ESI using a 22-gauge Tuohy needle at the lumbar spine through the interlaminar approach. The epidural space was identified by the loss of resistance technique with air. Results: The incidences of abnormal changes in MRI findings because of ESI were 54%, 31%, and 25% in patients who underwent MRI at approximately 24 h, and 2 and 3 days after ESI, respectively. Abnormal MRI findings included epidural air or fluid, needle tracks, and soft tissue changes. Epidural air, the most frequent abnormal finding (82%), was observed in 41% of patients who underwent MRI within 3 days after injection. Abnormal findings due to an ESI were not observed in MR images acquired 4 days after ESI or later. Conclusions: Pain physicians should consider the possibility of abnormal findings in MR images acquired after epidural injection using the interlaminar approach and the loss of resistance technique with air at the lumbar spine. (Korean J Pain 2017; 30: 281-6)

아아크가 抑制된 遮斷器의 開發에 關한 硏究

盧彰注,洪淳一,兪春植 釜山工業大學校 1984 論文集 Vol.26 No.-

The Authors studied how effectively the arcs are suppressed when the contactors are opened at current zero point which exists indispensively in AC current. In order to increase the suppressing effect in large current, the Authors also sutdied the effect when auxiliary contactors containing some resistance are connected with main ones and suggest how to determine the optimal value of resistance. According to the researched results, the Authors carried out the opening experiment of actual 3 phase current, 150A, 440V on the starting state of 5PH induction motor and confimed that the arcs of well adjusted phase currents are suppressed but others not so. The Author get the firm belief that if we construct in accurate mechanism, separate the contactors gear for each phase, and use the devices contrived by the Authors, the arcs of switch gear will be remarkably suppressed.

漢文 敎科書 內의 漢詩 分析 硏究 : 第7次 高等學校 漢文 敎科書를 중심으로

박완식,김창용,김상곤,김유태,정인덕 전주대학교 교육문제연구소 2001 敎育論叢 Vol.16 No.-

동종 조혈모세포이식 후 발생한 치명적 홍역 폐렴 1예

백창렬,이동건,최정현,정현화,조유경,박훈준,이승훈,박윤희,이교영,민우성,김춘추,신완식 대한감염학회 2001 감염 Vol.33 No.4

As a result of the enlarging pool of unvaccinated children and young adults, there has been an increase in measles in our countries. In these situation, it has been reported that measles associated pneumoinia is easily complicated with fatal respiratory failure, espycially in immunocompromised patients. Herein we report the case of lethal measles pneumonia after allogenic hematopoietic stem cell transplantation in adults proven by autopsy. Recently, one case of measles was encountered in 39-year-old female patients after allogenic bone marrow transplanted case (chronic myelogenous leukemia), who progressed into interstitial pneumonia pattern, despite treatment including antibiotics, immunoglobulin. The patient died of giant cell pneumonia compatible with that of measles which was comfirmed in the section of necropsy lung specimen. (Korean J Infect Dis 33:301∼309, 2001)

부인과 암수술에 있어서 외과의사의 역할

최평화(Pyong Wha Choi),유창식(Chang Sik Yu),정상훈(Sang Hun Jung),김대동(Dae Dong Kim),홍동현(Dong Hyun Hong),김희철(Hee Cheol Kim),김진천(Jin Cheon Kim) 대한외과학회 2008 Annals of Surgical Treatment and Research(ASRT) Vol.74 No.4

Purpose: Resection of the bowel or solid organs may be required for pelvic tumor surgery. The present study was performed to assess combined surgical procedures and determine the role of the general surgeon in gynecologic oncology surgery Methods: We performed a retrospective study of 135 patients with gynecologic malignancy who underwent a combined operation with a general surgeon between January 2000 and December 2005 at Asan Medical Center. The purpose of the combined operation was categorized into 3 groups: a tumor debulking operation, a resolution of intraoperative complications by a gynecologic surgeon, or an intraoperative diagnostic change. Results: The incidence of combined operations for debulking, resolution of intraoperative complications, and intraoperative diagnostic changes were 103 cases (76.3%), 22 cases (16.3%), and 10 cases (7.4%), respectively. Ovarian cancer was the most common gynecologic malignancy (74.1%) and the rate of a combined operation with a general surgeon in ovarian cancer was 18.5% during the time period. Colorectal resection was the most common procedure by a colorectal surgeon (61.5%). Twenty four patients (17.8%) experienced postoperative complications, including ileus, wound infection, pancreas leakage, and stomal necrosis, with no significant differences according to the purpose of combined operation, pathologic diagnosis, or bowel preparation. The rate of a preoperative consultation to a general surgeon by gynecologic surgeons in cases with colorectal or solid organ invasion preoperatively was 53.2%. Conclusion: A general surgeon is an important consultant in a debulking operation of gynecologic malignancies, especially ovarian cancer, or treatment of complications. Thorough preoperative evaluation, bowel preparation, and preoperative consultation to a general surgeon is important in gynecologic oncology surgery.

기술교과 교육 제도 및 연구의 국제적 동향 : ICTES '95 발표 논문을 중심으로 through Analyzing of Papers Presented at the ICTES '95

이재원,김진순,류창렬,노태천,최완식,최유현,송일민 충남대학교 공업교육연구소 1995 論文集 Vol.18 No.1

The purposes of this study were: (a) to analyze the papers presented at the ICTES according to the papers' representing countries and their main subjects, (b) to analyze the educational systems of the countries in technology education, and (c) to review the international trends of technology education through the papers presented at the conference. The papers(64 total) presented at the ICTES could be classified into eight countries of papers representing Japan(37), Taiwan(8), Korea(6), U. S. A.(3), India(3), Philippines(3), China(2), Singapore(2). The main subjects the papers dealt with could also be classified into educational system/history(9). curriculum/objectives and contents(9), international activities(9), methods/teaching-learning process and teaching materials and new media(40). Followings were the major and new issues presented at the ICTES: (a) Introduction of each country's technology education system. (b) Proposition of a new framework for technology education and a world core curriculum in technology education. (c) Creativity and problem-solving for the instructional method of technology education. (d) Methods and utilization of high technology media in technology education. (e) Necessity of engineering concept in technology education in technology oriented society. (f) Proposition of philosophical direction of technology education based on famous Asian philosophers.

활동성 궤양성 대장염 환자에서 발생한 뇌정맥 혈전증 1예

남승우,양석균,정훈용,주연호,홍원선,민영일,김종성,유창식,서대철 大韓消化器病學會 1998 大韓消化器病學會誌 Vol.32 No.3

상승온도 처리에 의한 논과 밭 용수의 질소변화

홍성창 ( Hong Sung-chang ),장은숙 ( Jang Eun-suk ),허승오 ( Hur Seung-oh ),최순군 ( Chio Soon-kun ),유선영 ( Yu Sun-young ),이규현 ( Lee Gyu-hyen ),김경식 ( Kim Kyeong-sik ) 한국환경농학회 2019 한국환경농학회 학술대회집 Vol.2019 No.-

Temperature increases due to climate change are affecting various sectors of agriculture. Elevated temperatures can affect the growth and yield of crops and can also affect the utilization efficiency of nutrient input materials such as soil nutrients, chemical fertilizers, and compost. The outflow of non-point pollutant sources from farmlands is strongly influenced by physical factors such as rainfall, rainfall intensity, and slope of agricultural land. In order to simulate the outflow of non-point pollutant sources due to climate change, it is necessary to find out not only the physical factors but also the changes in the biological factors induced by the elevated temperature in detail. Temperature is one of the most important environmental factors controlling the growth and yield of plants, and the rate of reaction depends on temperature in all biological processes. Elevated temperatures increase nitrogen mineralization and net nitrification rate. The degradation, absorption, utilization, and outflow of the variety of nutrient input materials for crop cultivation can differ due to temperature rise. This study was carried out to investigate the changes of nitrogen in the nutrients of the cultivated waters by cultivating rice and maize in pots after simulating climate change and by establishing an elevated temperature environment with the chemical fertilizer and livestock compost. The elevated temperature environment was established using the modified medium open-top chamber whose width is 6m and height is 3m. The medium open-top chamber has a merit of increasing only temperature while maintaining the environmental factors such as rainfall, wind, and sunlight intensity similar as those in the field. The maximum temperature in the open-top chamber measured on the 15th of May, 2018 was higher by 2.7℃ than the field, while the average temperature was higher by 0.4℃ so that the elevated temperature could be applied to the rice and maize throughout the growth period. The rice and maize were planted in Wagner pots filled with sandy loam and clay loam, and then placed in a medium open-top chamber and grown at elevated temperatures. The analysis results of nitrogen by periodically collecting the cultivation water from the Wagner pots during the cultivation period of the rice and maize showed that the NO₃―N concentration in the paddy cultivation water was decreased in the no-fertilization block, chemical fertilizer treated block, and cow dung compost treated block in the paddy cultivation sandy loam under elevated temperature compared to those in the field. Meanwhile, the NH₄― N concentration was increased in the rice cultivation clay loam soil in all the treated blocks compared to the field. The NO₃―N concentration in the cultivated water was decreased in all the treatment blocks in the maize cultivation clay loam soil by the elevated temperature than that of field. However, NH₄―N concentration in the water from the maize cultivation sandy loam soil, as well as the clay loam soil with the chemical fertilizer, was increased by elevated temperature compared to the field. The results indicate that the nitrogen changes in the water of the paddy field and upland are induced under the elevated temperature. The outflow of the non-point pollutant sources towards the water system near the cultivation fields also can be changed by rainfall.