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嫌氣性細菌 Erwinia carotovora subsp. carotovora에 의한 大麻皮의 精練
金永哲,金基淸,吳讚敎 全南大學校 農漁村開發硏究所 1991 農業科學技術硏究 Vol.26 No.-
後前의 Bacillus subtilis 菌에 의한 大麻皮의 纖維精練 方法을 改善하기 위해 Erwinia carotovora subsp. carotovora(植物 軟腐 病原菌)의 生育 培地別, 培地 pH別, 處理溫度別 및 處理時間別 pectin의 分解率과 現地 工場에서의 實用實驗의 精練程度 및 pectin 分解率에 基礎한 새로운 精練方法을 開發하였다. 1. 大麻皮 精練을 위한 E. carotovora의 利用에 있어서 母液培地로는 Nutrient broth나 0.5% pepton水보다는 最少無機염類培地가 pectin分解率에 있어서 가장 높았고 B. subtilis보다 pectin分解 活性이 훨씬 높았다. 2. Pectin分解率이 가장 높았던 E. carotovora 母液培地(最少無機염類培地)의 pH는 7.0에서 부터 8.0 사이였다. 3. E. carotovora를 利用한 大麻皮 精練 處理溫度는 30~35℃, 處理時間은 24~36時間이 合理的이었다. 4. 生産工場에서 實用試驗의 結果 B. subtilis 보다 E. carotovora가 精練效果에 있어서 越等히 좋았고, 1次 處理完了液을 그대로 2次 處理에 利用할 수 있었으며 보다 精練效率이 좋았다. 5. 以上의 結果에서 E. carotovora를 利用한 大麻皮 精練의 새로운 方法을 提示하면 다음과 같다. 菌母液으로는 最少無機염類培地(pH 7.0~8.0)를 利用하고 精練條件으로는 處理溫度 30℃, 處理時間 24~35時間, 連續 2回 處理하며, 强制합氣는 不必要하나 廢液의 滅菌處理가 必要하다. Fermentation conditions for the hemp refining by an anaerobic bacterium, Erwinia carotovora subsp. carotovora, were investigated. Percentage of the hemp pectin decomposition(% HPD) by the bacteria grown in the minimal broth, was measured as a indicator of fermentation since the % HPD was higher in the minimal broth than in the nutrient broth or 0.5% pepton water. The Optimal pH and temperature for the % HPD were pH 7.0~8.0 and 30~35℃, respectively. Fermentor test a factory demonstrated that E. carotovora is more effective organism than B. subtilis, which has been used up to now, for hemp refining at least in the condition of using minimal broth.
Jung, Chan-Kwon,Choi, Yeong-Jin,Lee, Kyo-Young,Bae, Ja-Seong,Kim, Hyung-Jin,Yoon, Seung-Kew,Son, Young-Ik,Chung, Jae Hoon,Oh, Young Lyun Mary Ann Liebert 2009 Thyroid Vol.19 No.8
<P>BACKGROUND: The cribriform-morular variant of papillary thyroid carcinoma (CMVPTC) is an unusual subtype of papillary thyroid carcinoma. The goal of this study was to determine the clinicopathological features of CMVPTC and whether the tumor can be diagnosed by fine-needle aspiration cytology. METHODS: We retrospectively analyzed the clinical appearance and pathological findings in five patients with CMVPTC and sequenced exon 3 of CTNNB1 and exon 15 of BRAF in tumor tissue. RESULTS: All patients were young women, 15-34 years of age at the time of the cancer diagnosis. Preoperative cytological examination showed scattered tall columnar cells, fascicular spindle cells, and cribriform and morular patterns in the fine-needle aspirates of the thyroid from the five patients. Grossly, all tumors were well-circumscribed, solid or cystic. Immunohistochemically, most tumor cells showed nuclear expression of thyroid transcription factor-1, estrogen and progesterone receptors, and p53; cytoplasmic expression of cytokeratins 7 and 19, vimentin, and bcl-2; and cytoplasmic and nuclear accumulation of beta-catenin and galectin-3. There was no expression of thyroglobulin, cytokeratin 5/6, or human mesothelial cell-1. However, among these markers, the morular cells showed only positive immunostaining for beta-catenin, galectin-3, p53, and bcl-2. A CTNNB1 mutation was identified in only one case and no BRAF mutation was found in any of the five cases. CONCLUSIONS: Taken together, these data suggest that CMVPTC can be diagnosed preoperatively, based on careful cytology examination, and shows unique immunohistochemical findings.</P>