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정기화,이현택,유차열,이상원 진주산업대학교 산업과학기술연구소 2007 산업과학기술연구소보 Vol.- No.14
4개소의 인공수정센터로부터 돼지정액을 제공 받아 돼지정액 중에 서식 중인 미생물을 순수분리하고, 그 미생물에 대한 항생제 감수성 검토를 행하였다. 2006년 7월과 9월에 채취한 시료의 원액에서 각각 3.6×10^(2)~2.6×10^(9) 및 3.8×10^(2)~5.5×10^(3)의 콜로니가 나타났으며, 희석정액의 보존일 수가 길어질수록 총균수가 많은 것으로 나타났다. 돼지정액 중에서 검출된 미생물로는 Bacillus sp., Staphylococcus sp. 및 E. coli와 같은 세균이 대부분이었으며, 그 이외에 Pseudomonas sp. 및 Norcardia sp. 등의 미생물들이 검출되었다. 이들 미생물에 대한 여러 종류의 항생제 감수성을 검토한 결과 enrofloxacin과 neomycin은 분리한 모든 미생물에 대하여 감수성을 나타내었으며, cefoxitin과 norfloxacin은 Norcardia sp.을 제외한 다른 미생물에 감수성을 나타내었다. Bacterial contaminants from boar semen, which was provided by the different 4 Artificial Insemination Centers in Korea, were isolated and investigated for antibiotic sensitivity. Bacterial flora were 3.6 × 10^(2) ~ 2.6 × 10^(9) CFU/mL and 3.8 × 10^(2) ~ 5.5× 10^(9) CFU/mL from raw boar semen collected from the period June ~ July to August ~ September 2006, respectively. Total viable cells of diluted boar semen were increased according to storage period. Isolated microbes were mostly detected as bacterial cells such as Bacillus sp., Staphylococcus sp. and Escherichia coli, followed by Pseudomonas sp. and Norcardia sp. All the microbes isolated from boar semen were sensitive to antibiotic enrofloxacin and neomycin. Norcardia sp. was only resistant and the others cells were sensitive to antibiotic cefoxitin and norfloxacin.
( Cha Yul Lee ),( Dong Ju An ),( Han Bit Lee ),( Kyung Yun Cho ) 한국미생물 · 생명공학회 2013 Journal of microbiology and biotechnology Vol.23 No.3
Phylogenetic analysis of the groEL1 and xynB1 gene sequences from Sorangium cellulosum strains isolated in Korea previously revealed the existence of at least 5 subgroups (A-E). In the present study, we used sequence analysis of polymerase chain reaction-amplified biosynthetic genes of strains from the 5 subgroups to indicate correlations between S. cellulosum subgroups and their secondary metabolic gene categories. We detected putative biosynthetic genes for disorazol, epothilone, ambruticin, and soraphen in group A, group C, group D, and group E strains, respectively. With the exception of KYC3204, culture extracts from group A, group B, and group C strains exhibited no noticeable antimicrobial inhibitory activities. By contrast, culture extracts from group D strains inhibited the growth of Candida albicans, whereas culture extracts from group E strains inhibited the growth of C. albicans and Staphylococcus aureus. High performance liquid chromatography analysis of the culture extracts from the strains of each subgroup revealed unique peak patterns. Our findings indicate the existence of at least 5 subgroups of S. cellulosum strains, each of which has the potential to produce a unique set of secondary metabolites.
사람 난포에서 angiotensin 2에 의한 steroidogenic pathway의 조절
이헌열 ( Hun Yul Lee ),고덕성 ( Duck Sung Ko ),김효정 ( Hyo Jung Kim ),오선옥 ( Sun Ok Oh ),박원일 ( Won Il Park ),홍서유 ( Seo Yoo Hong ),신정환 ( Jung Hwan Shin ),이진용 ( Jin Yong Lee ),차병헌 ( Byong Hun Cha ) 대한산부인과학회 2006 Obstetrics & Gynecology Science Vol.49 No.9
목적: 매우 많은 연구에도 불구하고 그 간접적인 방법 때문에 angiotensin II의 스테로이드 합성과정에 있어서의 역할에 대해서 잘 알려져 있지 않다. 이에 사람의 정상 난소 조직에서 난소의 renin angiotensin system이 안드로겐을 증가시키고 프로제스테론을 감소시킨다는 가설 하에 angiotensin II의 작용을 조사하였다. 연구 방법: 사람의 정상 난소 조직에 angiotensin II와 그 길항체인 saralasin을 처리한 후 progesterone, testosterone, DHEA 등의 sex steroid와 HSD3β2, CYP 17 등의 효소를 측정하여 angiotensin II와 saralasin의 작용을 조사하였다. 통계학적 유의성 검정은 ANOVA 방법을 사용하였으며, 실험군과 대조군 간의 p값이 0.05 미만인 경우에 통계적으로 유의한 것으로 판정하였다. 결과: angiotensin II는 androgen의 합성을 증가시켰으나 progesterone에는 영향이 없었다. HSD 3β2 mRNA의 발현은 angiotensin II와 saralasin의 군 간에 차이가 없었고, CYP17 mRNA의 발현은 angiotensin II를 처리한 군에서 증가하는 양상이었으나 유의한 차이는 없었다. 결론: 사람의 난소에서 angiotensin II는 CYP 17의 활성화에 의하여 androgen 합성을 증가시키며 progesterone의 합성에는 영향을 미치지 않는다고 사료된다. Objective: The exact mechanism of angiotensin II to steroidogenesis is still speculative in spite of many researches especially in human and these were performed indirectly with serum or follicular fluid. Under the hypothesis that ovarian RAS increases androgen, decreases progesterone synthesis in normal human ovary, we investigated the exact action of angiotnesin II on human ovary. Methods: After appliance of angiotensin II and saralasin to the normal human ovarian follicles, we measured sex steroids like progesterone, testosterone, DHEA and enzymes like HSD3β2, CYP 17 to see the action of angiotensin II and its antagonist, saralasin. The results were analyzed by ANOVA test. Results: Angiotensin II increased androgen synthesis but did not affect progesterone synthesis. There were no difference of HSD 3β2 mRNA expression in angiotensin II and saralasin group compared with control group. The expression of CYP17 mRNA was increased by angiotensin II but did not reach statistically significant level. Conclusion: Angiotensin II could increase androgen production probably via overexpression of CYP17, but had no efffect on progesterone production.
김치로부터 분리한 Leuconostoc citreum GR1의 마우스에 대한 급성독성
이환 ( Hwan Lee ),차선숙 ( Seon Suk Cha ),이명렬 ( Myung Yul Lee ),장해춘 ( Hae Choon Chang ),이재준 ( Jae Joon Lee ) 한국식품저장유통학회(구 한국농산물저장유통학회) 2013 한국식품저장유통학회지 Vol.20 No.1
The present study was carried out to investigate the in vivo single-dose toxicity of Leuconostoc citreum GR1 , a ;actic acid bacteria isolated from kimchi, in ICR male and female mice. The test article was orallly adminisyered once to both sexes of mice. The mortalities, clinical findings, autopsy findings, and body weight changes were monitored daily for two weeks. The male and female mice were gavaged with Leuc. kimchi GRI of four doses. The oral LDso of Leuc. citreum GRI was considered higher than 5,000 mg/kg. Nosignificant changes in the general conditions, body weights, clinical signs and presence of gross lesions were boserved in both sexes of mice to whom Leuc. cireum GRI was administered orally. The results indicated that the 5,000 mg/kg dose of Leuc. citreum GRI showed no adverse effect.
성인둔상환자에서 평균동맥압과 위해사건발생의 관련성: 단면 조사 연구
차승용 ( Seung Yong Cha ),김용환 ( Yong Hwan Kim ),홍종근 ( Chong Kun Hong ),이준호 ( Jun Ho Lee ),조광원 ( Kwang Won Cho ),황성연 ( Seong Youn Hwang ),이경렬 ( Kyoung Yul Lee ),이영환 ( Young Hwan Lee ),최성희 ( Seong Hee Choi 대한외상학회 2013 大韓外傷學會誌 Vol.26 No.2
Purpose: Non-invasive blood pressure measurement is widely used as a pre-hospital triage tool for blunt trauma patients. However, scant data exits for using the mean arterial pressure (MAP), compared to the systolic blood pressure, as a guiding index. The aim of this study was to determine the association between adverse outcomes and mean arterial pressure (MAP) and to exhibit the therapeutic range of the MAP in adult blunt trauma patients. Methods: The electronic medical records for all trauma patients in a single hospital from January 2010 to September 2012 were retrospectively reviewed. Patients below 17 years of age, patients with penetrating injuries, and patients with serious head trauma (injuries containing any skull fractures or any intracranial hemorrhages) were excluded. Adverse outcomes were defined as one of the following: death in the Emergency Department (ED), admission via operating theater, admission to the intensive care unit, transfer to another hospital for emergency surgery, or discharge as hopeless. Results: There were 14,537 patients who met entry criteria. Adverse outcomes occurred for MAPs in range from 90 to 120 mmHg. Adverse outcomes were found, after adjusting for confounding variables, to occur increasingly as the MAP declined below 90 mmHg or rose above 120 mmHg. Conclusion: Not only lower but also higher mean arterial pressure is associated with increased adverse outcomes in adult blunt trauma patients. Thus, patients with a MAP above 120 mmHg should be considered as a special group requiring higher medical attention, just as those with a MAP below 90 mmHg are.