Alcoholics 환자와 Alcohol Dehydrogenase

강병조,이종학,신창규,정태호 대한생물치료정신의학회 1995 생물치료정신의학 Vol.1 No.2

저자들은 만성알콜중독의 원인으로 음주자의 성격, 사회요인 등 여러가지 문제들이 게재되어 있지만 유전적 요인도 중요하리라고 생각되어 만성알콜중독자로 진단되어 국립부곡정신병원에 입원가료 중인 환자 30명을 대상으로 ADH2의 다형현상(polymorphism)을 조사하여 알콜중독자가 아닌 건강한 한국인의 유전자형과 비교한 결과 다음과 같은 결과를 얻었다. 만성알콜중독자 30명 중 ADH2-1/ADH2-1 형을 갖는 사람이 27명이었으며 ADH2-1/ADH2-2를 갖는 사람이 3명이었다. ADH2¹의 gene frequencies는 0.68이었으며 ADH²의 gene frequencies는 0.32로서 정상 한국인과 비교하여 유의한 차이가 없었다. This study was performed to know the gene polymorphism of alcoholic dehydrogenase(ADH)-2 in 30 Korean alcohol addicted patients who were admitted at Bugok National Mental Hospital. The results were as follows ADH2-1/ADH2-1 type among 30 alcohol addicts were 27 and ADH2-1/ADH2-2 type were 3. Gene frequencies of ADH2-1 were 0.68 and gene frequencies of ADH2-2 were 0.32 These results were not different from the gene polymorphism of ADH2 in non-alcoholic Korean healthy adults.

미세채널 구조물 상부의 초정밀 연마 기술 연구

강정일,이윤호,안병운,윤종학 한국공작기계학회 2003 한국공작기계학회 추계학술대회논문집 Vol.2003 No.-

Micro-Channel ultra-precision polishing is a new technology used in magnetic field-assisted polishing. In this paper, an electromagnet or the jig of test system was designed and manufactured. A size of magnetic abrasive is used on 25~75μm and for the polish a micro-channel upper part. A surface of channel which is not even is manufactured using magnetic abrasive finishing at upper surface of micro-channel. As a result, the surface roughness rose by 80% after upper surface of micro- channel was polished up 8 minutes by polishing.

Rat의 복강내에 투여한 5-fluorouracil-polyglycolic acid 제형이 장기에 미치는 영향에 관한 연구

강대영,송규상,최정목,노승무,정경수,오정연,김진향,양준묵,최선웅,이진호,조준식,민병무,김용백,김창식,박근성,김승영,김학용,인현빈 충남대학교 의과대학 지역사회의학연구소 1998 충남의대잡지 Vol.25 No.1

For more effective intraperitoneal chemotherapy, the authers made 5-fluorouracil-polyglycolic acid composite disks(FU-PGA disks) with 5-fluorouracil and polyglycolic acid, a biodegradable polymer. We inserted the FU-PGA disk into the peritoneal cavites of Sprague-Dawley male rats, the control and three experimental groups; one FU-PGA disk insert group(300mg/kg), two FU-PGA disk insert group(600mg/kg), and three FU-PGA disk insert group(900mg/kg). The control group received a similar number of PGA disk inserts. A pharmacokinetic study was performed to measure the 5-fluorouracil concentration in the peritoneal fluid, blood, and tissues(liver, kidney and heart) at 24 hours, 72hours and 168 hours after insertion of the FU-PGA disk. Light and electron microscopic studies were done. The results were as follows: 1) The number of white blood cells and platelets decreased after FU-PGA insertion. The degree was proportional to the duration and amounts inserted. The change in the number of red blood cells varied slightly. 2) Light microscopically, slight changes were noted at 168 hours in the 3 disk insert group (900mg/kg). Mild fatty change and hepatocyte degeneration around the central veins of the liver were noted, with vacuolar degeneration in the proximal convoluted tubules of the kidney. 3) Electron microscopically,the liver showed focal increases of lipid droplets in the hepatocytes, and irregular nuclear membrane with focal nucleolar segregation of the fibrillar and granular elements. Also the double membranous structure of the hepatocytes and Kupffer cells was widened with bleb formation. The kidney showed wide separation of the nuclear double membrane in the proximal convoluted tubular cells and mesangial cells, with vacuolation and myelin figure formation in the mitochondria of the proximal convoluted tubular cells, at 72 hours and 168 hours in the 2 to 3 FU-PGA insert groups. The heart showed focal loss of cristae,vacuolation and myelin figure formation in the mitochondria of the 2 to 3 FU-PGA insert groups. In conclusion, despite the large amont of FU-PGA inserted, the histopathological changes in the liver, kidney and heart were slight, and consonant with the very low amount of 5-fluorouracil concentrations detected in the liver, kidney and heart. The above results suggest that the FU-PGA composite can serve as a new device for releasing drugs in a controlled manner and easily targeted to intraperitoneal organs. This device can improve the efficacy of intraperitoneal chemotherapy for gastric cancer.

생체분해성 고분자를 이용한 사시수술용 제형개발 및 효용성에 대한 연구

민병무,김용백,김승영,김창식,박근성,길숙종,조항진,이성복,노승무,송규상,강대영,조준식,양준묵,정경수,최선웅,이진호,김학용,인현빈 충남대학교 의과대학 지역사회의학연구소 1998 충남의대잡지 Vol.25 No.1

A new device, muscle clamping system was developed to facilitate exact quatifying technique A strabismus surgery, and reduce the risk of complications. The device is composed of a lower fixing body with three jaws and an upper supporting body. They are used to clip an extraocular muscle and fix it to the sclera with a single bite. Superior rectus recession on 16 rabbit eyes were performed with this new device. Conjunctival injection, muscle adhesion strength, and light microscopic findings were examined at 1, 2, 4 and 8 weeks postoperatively. The Conjunctival injection were minimal, adhesion power ranged from 420 to 600 gram gravity, which is sufficient in withstand the normal pull of human extraocular muscle. In microscopic exam, some Inflammatory cells and fibrosis were found. The new device was technically easy, fast, and accurate, so it may be useful in stabismus surgery.

미세입자(PM_(2.5))의 배출원 구성물질 성분비 개발에 관한 연구

이학성,강충민,강병욱,이상권 한국대기환경학회 2004 한국대기환경학회지 Vol.20 No.3

The purpose of this study was to develop the PM_(2.5) source profiles, which are mass abundances (fraction of total mass) of a chemical species in PM_(2.5) source emissions. The source categories studied were soil, road dust, gasoline and diesel vehicles, industrial source, municipal incinerator, coal-fired power plant, biomass burning, and marine. The chemicals analyzed were ions, elements, and carbons. From this study. soil source had the crustal components such as Si, AI, and Fe. In the case of road dust, Si, OC, Ca, Fe had large abundances. The abundant species were SO₄^(2-), Cl^(-), NH₄^(+), and EC in the gasoline vehicle and EC, OC, Cl^(-), and SO₄^(2-) in the diesel vehicle. The main components were SO₄^(2-), S, NH₄^(+), and EC in the industrial source using bunker C oil as fuel. ^(-), NH₄^(+), Fe, and OC in the municipal incinerator source, and Si, Al, SO₄^(2-), and OC in the coal-fired power plant source. In the case of biomass burning, OC, EC, and CI- were mainly emitted. The main components in marine were Cl^(-), Na^(+), and SO₄^(2-).

백서의 복강내에 투여한 Mitomycin-C-Polyglycolic acid 제형이 장기에 미치는 영향에 관한 연구

송규상,강대영,최정목,노승무,정경수,오정연,김진향,양준목,최선웅,이진호,조준식,민병무,김용백,김창식,박근성,김승영,김학용,인현빈 충남대학교 의과대학 지역사회의학연구소 1998 충남의대잡지 Vol.25 No.1

To evaluate the in-vivo effects of mitomycin-C-polyglycolic acid composite(MMC-PGA) the disk: shaped composite were implanted into the peritoneal cavity of the Sprague-Dawley rats. The pathologic changes were examined at various time points up to 12 weeks. Initially the soft tissue around the inserted disks showed capillary congestion at 3 days. Inflammatory infiltrates with foreign body giant cells appeared from the 2nd week and reached peak response at 6-8 weeks. These reaction diminished prominently at 12 weeks. No specific pathologic change was found in the liver, the kidneys, and the heart. The above results suggest that the MMC-PGA composite can serve as a new device for intraperitoneal chemotherapy of various types of cancers.

휘발성유기화합물의 배출원 구성물질 성분비에 관한 기초 연구

이영재,이학성,강병욱,신대윤 한국환경과학회 2003 한국환경과학회지 Vol.12 No.4

The purpose of this study was to develop the preliminary source fingerprints of volatile organic compounds(VOC). The source categories studied were vehicles, gasoline vapor, gasoline storage tank, coating, dry cleaning and road covering. The source samples were collected using 6L electro-polished stainless steel canisters for about 20 seconds. From this study, the main component emitted from VOC sources in Korea was toluene. The toluene proportion for road covering, vehicles, coating and gasoline vapor were 35, 18, 16 and 5%, respectively, The C_2-C_5 alkane and alkene compounds were mainly emitted from vehicles, gasoline vapor and gasoline storage tank, The main compounds of coating were m/p-xylene(34%), toluene(16%), 1,2,4-TMB(22%) and 1,3,5-TMB(13%) were mainly emitted.

생체분해성 망막압정을 이용한 망막고정에 대한 실험적 연구

김용백,민병무,김창식,박근성,김승영,길숙종,조항진,이성복,노승무,송규상,강대영,조준식,양준묵,정경수,최선웅,이진호,김학용,인현빈 충남대학교 의과대학 지역사회의학연구소 1998 충남의대잡지 Vol.25 No.1

Biodegradable retinal fixation devices obtain mechnical fixation of the retina with desirable chorioretinal scarring and with the potential for local, sustained release of antimetabolites and steroids to inhibit proliferative vitreoretinopathy. We manufactured a biodegradable retinal tack with barb that was designed in order to prevent intrusion from implantation of retinal tacks. This study was carried to evaluate the efficacy for retinal fixation and the capability for sustained release of drugs with a newly designed biodegradable retinal tack Biodegradable retinal tacks were made of polymers of glycolic acids and were designed with barbs in a shape to prevent the disinsertion. Biodegradale retinal tacks are divided into 3 parts, a conical portion that is inserted into the sclera, a cylinder portion that remains in the vitreous, and a neck portion between the pin and the cylinder. The tapered conical end was manufactured to allow easy insertion through the retina and choroid into the sclera. A cylinder portion was manufactured with a tapered angle that fixes firmly into the orifice of 19 gauge spinal needle. A neck portion, 0.4 mm in diameter, was designed to prevent disinsertion from following implantation of retinal tack. The applicator was a 19 gauge spinal needle and its orifice was prepared to 15°angle to accept the tapered cylinder portion of the retinal tack. The retinal tacks, secured in the needles, were passed through the formed vitreous and inserted into the retina, choroid, and sclera and were released by pushing the internal needle, usually within 2-3mm of the medullary ray of the posterior rabbit retina A retinal tack was placed in each of 8 pigmented rabbit eyes. Slit-lamp biomicroscopy, indirect ophthalmoscopy and fundus photography were performed periodically from 1 day to 8 weeks after surgery. Eight eyes were enucleated and studied by light microscopy at 8 weeks. Biomicroscopic evaluation of the animals revealed edemas adjacent to the retinal surfaces immediately after insertion of the biodegradable retinal tacks in all the animals. These edemas disappeared after 1 week. The first noticeable change in the size of retinal tacks was shown after 2weeks. The size of the retinal tacks gradually got smaller, decreasing to about one-half at 4 weeks and about one-third at 8 weeks. All retinal tacks remained in inserted places without any movement for an 8 week period. On light microscopy, epiretinal proliferations were seen to extend into the vitreous cavity. Cellular capsules that lined the inner aspect of the scleral defect caused by tack insertion were found. However the adjacent retina had a normal cytologic appearance and architecture in all specimens. We manufactured a biodegradable retinal tack that is designed to prevent intrusion from implantation of retinal tacks. All biodegradable retinal tacks reduce in size with time, but no retinal tacks extruded from the inserted place. The newly designed biodegradable retinal tack can be used for retinal fixation and may be used as a vehicle for the introduction of pharmacologic agents to prevent the cellular events that promote proliferative vitreoretinopathy.

정신분열병의 유병기간과 인지적 결함간의 관계

이근창,이종학,강병조 대한생물치료정신의학회 1996 생물치료정신의학 Vol.2 No.1

정신분열병에 대한 현행의 개념이 손상된 인지기능이 이 질환의 정신병리중의 하나라고 받아들여지고 있다. 그러나 정신분열병의 경과가 점진적이고 지속적인 손상을 유발하는 진행적인 치매적 장애인지 급성 또는 아급성과정에서 인지기능의 감소가 오고 결국은 비교적 안정된 결함 상태가 되는 정적뇌병증인지에 대한 논란은 아직도 계속되고 있다. 본 연구에서는 58명의 정신분열병환자들을 유병기간에 따라 5년이하(Group 1), 5년초과에서 10년이하(Group 2), 10년초과에서 15년이하(Group 3), 15년초과에서 20년이하(Group 41)의 4군으로 분류하여 정신분열병환자들에서 유병기간이 증가함에 따른 인지기능의 차이가 나타나는 지에 대해서 WCST, VCPT, 재인기억검사, 공간기억검사, 수지럭검사, KWIS 어휘문제 및 BPRS를 시행하여 다음과 같은 결과를 얻었다. 1) 각 군간에서의 간이정신평가척도(F=0.22, d.f.=3,54, P=0.88)는 유의한 차이가 없었다. 2) Wisconsin카드분류검사(F=0.23, d.f.=3,54, p=0.87), 시각지속수행검사(F=0.27, d.f.=3,54, P=0.84), 공간기억검사(F=0.10, d.f.=3,54, P=0.96), 재인기억검사(F=0.15, d.f.=3,54, p=0.93) 수지력검사(F=1.20, d.f.=3,54, p=0.32), 어휘문제(F=0.67. d.f.=3,54, P=0.98) 등 실시한 모든 신경심리검사에서 4군간에 유의한 차이가 없었다. 이러한 결과로 볼 때 정신분열병은 유병기간과는 무관한 정적뇌병증일 것이라는 것을 시사한다. Most current thinking about schizophrenia acknowledges that impaired cognitive performance is one of the manifestations of the neuropathology of the illness. But, controversy surrounds the issue of whether a dementing disorder which produces a gradual and progressive loss of cognitive function or a static encephalopathy which is a relatively stable deficit state followed after cognitive function diminishing during an acute or subacute process. In this study, 58 schizophrenic patients were assigned to four duration of illness-derived groups: duration of illness≤5years(group 1), 5<duration of illness≤10years(group 2), 10<duration of illness≤15years(group 3), 15<duration of illness≤20years(group 4). To ascertain the significant differences of the cognitive function between 4 duration of illness-derived groups, WCST, VCPT, recognition test, spatial memory, finger tapping test, KWIS-vocabulary test, and BPRS were performed. The results are as follows ; 1) There was no significant differences between 4 groups on BPRS(F=0.22, d.f.=3,54, P=0.88). 2) There were no significant differences between 4 groups on WCST(F=0.23, d.f.=3,54, P=0.87), VCPT(F=0.27, d.f.=3,54, P=0.84), spatial memory(F=0.10, d.f.=3,54, P=0.96), recognition test(F=0.15, d.f.=3,54, P=0.93), finger tapping test(F=1.20, d.f.=3,54, P=0.32), KWIS-vocabulary test(F=0.67, d.f.=3,54, P=0.98). These results suggest that the course of schizophrenia is more consistent with a static encephalopathy than a dementing disorder, irrespective of duration of illness.

Alcoholics 환자와 Alcohol Dehydrogenase

정태호,신창규,이종학,강병조 경북대학교 병원 1998 경북대학교병원의학연구소논문집 Vol.2 No.1

저자들은 만성알콜중독의 원인으로 음주자의 성격, 사회요인 등 여러가지 문제들이 게재되어 있지만 유전적 요인도 중요하리라고 생각되어 만성알콜중독자로 진단되어 국립부곡정신병원에 입원가료 중인 환자 30명을 대상으로 ADH2의 다형현상(polymorphism)을 조사하여 알콜중독자가 아닌 건강한 한국인의 유전자형과 비교한 결과 다음과 같은 결과를 얻었다. 만성알콜중독자 30명 중 ADH2-1/ADH2-1 형을 갖는 사람이 27명이었으며 ADH2-1/ADH2-2를 갖는 사람이 3명이었다. ADH2¹의 gene frequencies는 0.68이었으며 ADH²의 gene frequencies는 0.32로서 정상 한국인과 비교하여 유의한 차이가 없었다. This study was performed to know the gene polymorphism of alcoholic dehydrogenase(ADH)-2 in 30 Korean alcohol addicted patients who were admitted at Bugok National Mental Hospital. The results were as follows ADH-2/ADH2-1 type among 30 alcohol addicts were 27 and ADH2-1/ADH2-2 type were 3. Gene frequencies of ADH2-1 were 0.68 and gene frequencies of ADH2-2 were 0.32 These results were not different from the gene polymorphism of ADH2 in non-alcoholic Korean healthy adults.