소 수정란의 공배양 및 동결이 체외발생에 미치는 영향에 관한 연구

이종진,이명헌,남윤이,이만휘,이봉구,김상근 충남대학교 수의과대학 동물의과학연구소 1996 動物醫科學硏究誌 Vol.4 No.-

The studies on the carried out to investigate the effects of co-culture with cumulus cells and oviduct epithelial cells on the in vitro fertilization and cleavage rate of bovine follicular oocytes, and to determine the optimum thawing temperature and equilibration time of frozen bovine embryos and on survival rate and in vitro developmental rate of bovine embryos. The ovaries were obtained from slaughtered Korean native cows. The follicular oocytes were cultured in TCM-199 medium containing 10 IU/㎖의 PMSG(Sigma, USA), 10 IU/㎖의 hCG, 1 ㎍/㎖의 β-estradiol(Sigma, USA) and 10% FCS(Sigma, USA) for 24-48 hrs in incubator with 5% CO_2 in air at 38.5℃ and then matured oocytes were again cultured for 12-18 hrs with motile capacitated sperm by preincubation of heparin. The bovine embryos following dehydration by cryoprotective agents and a various concentrations of sucrose were directly plunged into liquid nitrogen and thawed in 30℃ water. The results are summarized as followes : 1. The in vitro fertilization and in vitro developmental rates of bovine oocytes co-cultured with cumulus cells in TCM-199 media were 75.0%-76.8% and 17.3%-27.6%, respectively. And in-vitro fertilization rates of cumulus-enclosed oocytes(55.4%) were significantly(p<0.05) higher than cumulus-denuded oocytes(23.1%). 2. The in vitro fertilization and in vitro developmental rates of bovine oocytes co-cultured with 1 ×10^4 cells/㎖, 1 ×10^6 cells/㎖, 1 ×10^8 cells/㎖ and 1 ×10^15 cells/㎖ oviduct epithelial cells in TCM-199 media were 74.5%-77.8% and 15.7%-21.2%, respectively. 3. The in-vitro fertilization and in vitro developmental rates of bovine oocytes co-cultured in TCM-199 media containing PMSG, hCG, PMSG + hCG, PMSG + β-estradiol, hCG + β-estradiol 0 to 40 hrs after insemination were 74.0%-77.4% and 18.9% - 23.1, respectively. 4. The survival rates of bovine embryos thawed after rapid freezing in the media containing various kinds of cryoprotective agents added 0.25M and 0.50M sucrose were 14.7% - 35.1% and 17.6% - 31.6%, respectively. The survival rates of bovine embryos thawed after rapid freezing in the freezing media containing a various concentration of sucrose added 1.5M and 2.0M glycerol, 1.5M and 2.0M DMSO, and 1.5M and 2.0M propanediol were 23.5% -31.4% and 20.6% - 34.1%, respectively. 5. The temperature thawed at 30℃ after rapid freezing of bovine embryos resulted in a significantly higher embryos survival rate than did at 20℃ and 35℃. 6. The equilibration time on the survival rates of bovine embryos was attained after short period of time(2.5-5 min.) in the freezing medium higher than ling period of time(10-20min.).

대학생의 기본 심폐소생술 교육 평가 : 간호과와 비간호과를 대상으로

이정은,고봉연,이인모,최근명,박신일,안홍기 한국응급구조학회 2003 한국응급구조학회지 Vol.7 No.1

The purpose of this study was to evaluate the accuracy of CPR skills and the satisfaction of CPR Training targeted for college students. Also, this study made comparative study of nursing students and non-nursing students. The sample consisted of 248 students(132 nursing students, 116 non-nursing students). CPR Training was designed by two components which were a lecture and demonstration by one professor and individual practice 표 instructors. As tool of measurement estimation satisfaction of CPR Training questionnaires were developed based on educated contents. The accuracy of CPR skills were checked by SkillReporter CPR training manikin. Collected data were computerized and analyzed by SPSS-WIN program. The results were as follows : 1. The skills of CPR were cardiac compression 92.79 times/min, correct cardiac compression rate 90.85%, ventilation 9.93 times/min. correct ventilation rate 79.34%. 2. The mean of the accuracy of CPR skills were 2.94(SD .87). 3. When errors were analyzed, the highest item was stomach distension(51.2%) of ventilation skills and too little(70.6%) of cardiac compression skills. 4. The mean of the satisfaction of CPR training were 2.87(SD .17), the highest item of the satisfaction of CPR Training was practice. 5. We found significant statistical differences based on the accuracy and the error, non-nursing students were high in correct cardiac compression per minute(t=3.615, p=0...) and ventilation too much(t=4.292, p=.000), nursing students were high in correct ventilation rate(t=-3.885, p=.000) and cardiac compression too shallow) t=-2.842, p=.005).

정상 및 Phenylhydrazine으로 처치한 토끼 적혈구의 Adenosine Deaminase Isozymes의 활성도에 관하여

李鉉國,車鍾希,李奉宰,金良均,李根培 조선의대 1984 The Medical Journal of Chosun University Vol.9 No.1

용접공들의 면역능에 관한 연구

이수일,조봉수,김영욱,고광욱,조원근,김영실,강수용,황인경,조병만,김돈균 大韓産業醫學會 1997 대한직업환경의학회지 Vol.9 No.1

The authors examined human immunity indicators such as CD4, CD8, Natural Killer(NK) cell, IgG, IgA, and IgM, and heavy metals such as Pb, Cr, Cd to evaluate the health hazard of welders. We selected total 126 male subjects, 60 welders who had more than 5 year working history as an exposure group, and 66 office workers and medical college students as a control group. This immunity evaluation approach is the first newly designed study that has never been tried in past studies on welder's heath evaluation. And the results were as follows. 1. Total lymphocyte count of peripheral blood was significantly higher in welder group, 2,615±650 ea/㎕ compared with 2,368±681 ea/㎕ in control group(p>0.05). 2. The proportion of CD4, CD8, NK cell in welder group was 37.5±9.4%, 24.0±8.8%, and 21.0±9.5%, respectively, and 35.5±6.5%, 25.0±6.9%, and 19.6±8.8% in control group. CD4 and NK cell were slightly higher, and CD8 was slightly lower in welder group. but there was no statistical significance between two groups(p≥0.05). 3. IgG, IgA, and IgM in welder group were 15.9㎎/㎗, 2.7㎎/㎗ and 1.5㎎/㎗, respectively, and 15.6㎎/㎗, 2.9㎎/㎗ and 1.7㎎/㎗ in control group. There was no statistical significance between two groups(p≥0.05). 4. Blood lead, cadmium and chromium concentrations were 4.2±1.8㎍/㎗, 0.4±0.38㎍/㎗, 1.2±0.6㎍/㎗ in welder group and 3.2±1.7㎍/㎗, 0.44±0.29㎍/㎗, 1.1±0.6㎍/㎗ in control group, respectively, and these value were within normal ranges.

유도 지도자의 리더십 유형이 팀 성과에 미치는 영향

이기철,이봉건,안철순 부산 외국어 대학교 2001 外大論叢 Vol.23 No.1

With high school judo athletes as subjects, this study aims to reveal the relations between leadership types they favor and those they actually perceive. This analysis of the degrees of discrepancy and satisfaction felt by the athletes led to the following conclusions: 1.Leadership score according to sexes: 1) Autocratic behavior was the highest and affirmative feedback was the lowest in male teams. 2) Social support was the greatest and instructions appeared the lowest in female teams. 2.Leadership score in male teams according to team levels: 1) In superior teams, the highest and the lowest were social support and instructions respectively. 2) In inferior teams, autocratic and democratic behavior scored the highest and the lowest marks respectively. 3.Leadership score in female teams according to team levels: 1) In high-achievement teams, the highest and the lowest were social support and positive feedback respectively. 2) In low-achievement teams, autocratic behavior and instructions scored the highest and the lowest marks respectively. 4.Leadership discrepancy and athletes' satisfaction according to sexes: 1) The male group with lower discrepancy in instractions (p<0.01) and democratic behavior (p<0.001) showed greater satisfaction. 2) The female group with lower disagreement in autocratic behavior(p<0.01) indicated higher degrees of satisfaction. 5.Leadership behavior types and athletes' satisfaction degrees according to sexes and team levels: 1) In male teams, the excellent group in instructions and democratic behavior (p<0.01) revealed more satisfaction. 2) In female teams, the outstanding group in instructions (p<0.001), democratic behavior, and autocratic behavior (p<0.01) tuned out to be more satisfied.

표면 와류 분포 방법을 이용한 2차원 캐스케이드 설계에 관한 연구

李根明,金學奉 한국항공대학교 1986 論文集 Vol.24 No.-

A method of calculation in designing two-dimensional cascades with given velocity distribution is developed. An iterative method of the solution of the integral equation to determine the coordinate function for the blade profile is presented. We assume that the incompressible potential flow past two-dimensional cascades is a channel flow. The surface vortex distribution method is used to simulate the cascade channel flow. Some design examples for compressor and turbine cascades with given flow angles and prescribed velocity distribution are presented. The results obtained by the surface vortex distribution method show a good agreement with those of other solution methods and with actual blades.

돼지 우리 깔집용 왕겨에서의 Ochratoxin A 검출

이문한,박진봉,박응복,이영재,신광순,박종명,남궁선,조준형,박근식,권택석,이재구 한국수의공중보건학회 1992 예방수의학회지 Vol.16 No.1

각속도 변동 지수와 크랭크각 지수를 이용한 가솔린 기관의 실화 검출

이근봉,김세웅,심국상 國立金烏工科大學校附設生産技術硏究所 1997 産業技術開發硏究 Vol.13 No.-

This paper introduces a diagnostic technique for the detection of faulty cylinders of internal combustion engines, using the indexes of the angular velocity variation and the crankshaft angle. Many types of faults may occur during the operation of internal combustion engines. These include low compression pressure, failure of ignition or combustion of malfunction of the fuel system. Early detection and correction of engine faults restore proper performance, improve engine efficiency and reduce costs. From the idea that the fluctuation of crankshaft speed contains the information of combustion phenomena, angular velocity variation is measured, with an interval timer and a magneric pick-up sensor, by timing the flywheel gear teeth as they pass the sensor. The angular velocity and angular acceleration are determined by cylinder pressure, load, inertia, and friction of the engine. The angular acceleration patterns, as well as the velocity patterns, are varied according to the relative effects between cylinder pressure and reciprocating inertia. Therefore the patterns are classified into three regions; the inertia dominant region, the combined region, the pressure dominant region. In this paper, misfire detection was investigated according to the regions with parameters defined for misfire detection. By this method, the single misfire or multiple misfires can be detected

初乳의 分泌期間中의 成分變化 및 保存性에 관한 硏究

李鳳求,金相根,李晩徽,金武剛 충남대학교 수의과대학 동물의과학연구소 1995 動物醫科學硏究誌 Vol.3 No.-

The study was carried out to investigate the charges of hourly and daily components and preservation of colostrum produced 1st through 7th day after parturition of Holstein cows. The results obtained were summarized as follows : 1. The specific gravity in colostrum was 1.032-1.057 from 1 to 24 hrs after parturition and then showed slightly higher than normal milk. The pH and titerable acidity were 6.43 and 0.170% at 1 hrs after parturition respectively and thereater gradually increased. 2. The solids not-fat, fat and protein of colostrum were 16.24%, 6.60% and 14.20% at 1hrs after parturition respectively, and then showed markedly higher than normal milk. 3. The solids not-fat, fat and protein of colostrum were 9.02%, 6.66% and 3.92% at day 1 postpartum respectively and thereafter gradually decreased. But milk lactose ranged from 2.47% to 4.76% at day 1-7 postpartum and thereafter gradually increased. 4. The F, Mg and Fe of colostrum were 0.244%, 0.064% and 0.430% at day 1 postpartum respectively. The Ca and Na were 0.262% and 0.074% at day 1 postpartum and then showed markedly higher than normal milk and thereafter gradually decreased. 5. The pH from 1st through 5th days of milking was decreased from 6.4 to 5.3. The number of common bacteria reached a peak levels of 1×10^7.5/ml after 3-7 days and then slightly decreased. The number of coli from group reached a peak levels of 1×10^8/ml after 3 days and thereafter rapidly decreased to 10^3/ml after 20 days. 6. The number of common bacteria of colostrum added 0.5, 1.0 and 2.0% of propionic acid and acetic acid at 20℃ were inhibited from 1 to 20 days in addition of 0.5%-2% propionic acid and acetic acid. The number of coli form group was retarded from 3 to 7 days addition of 1.0%-2.0% propionic acid and acetic acid. The addition of propionic acid and acetic acid to colostrum were effective in retarding the growth of bacteria than acetic acid.

비보강 조적벽체 전단내력산정에 관한 연구

이봉근,이정한,이원호,오상훈,나정민 대한건축학회 2003 대한건축학회 학술발표대회 논문집 - 계획계/구조계 Vol.23 No.1(구조계)

In Korea, more than four hundred earthquakes with medium intensity centered on off-coastal and inland of Korea during the past 20 years and due to the great earthquakes which occurred recently in neighboring countries the importance of the future earthquake preparedness measures in Korea is highly recognized. More than 80 percents of residential buildings are constructed with unreinforced masonry structure, in Seoul. In general, unreinforced masonry structure don't have enough strength against the lateral force. And low rise buildings such as unreinforced masonry structure didn't adopted seismic design. So, a huge damage is expected when an earthquake occurs. It is that the major variables of shear strength are the aspect ratio, the vertical strength and the method of masonry. The objectives of this study are, 1) to find out the shear behavior of masonry wall and the variables affect the shear capacity of masonry wall such as aspect ratio of the unreinforced masonry wall, and the vertical strength, 2) to propose the equation of the shear capacity of masonry wall by regression analysis using the test data.