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Ding Sheng-Long,Zhang Tai-Wei,Zhang Qi-Chen,Ding Wang,Li Ze-Fang,Han Guan-Jie,Bai Jin-Song,Li Xi-Lei,Dong Jian,Wang Hui-Ren,Jiang Li-Bo 생화학분자생물학회 2021 Experimental and molecular medicine Vol.53 No.-
Night shift workers with disordered rhythmic mechanical loading are more prone to intervertebral disc degeneration (IDD). Our results showed that circadian rhythm (CR) was dampened in degenerated and aged NP cells. Long-term environmental CR disruption promoted IDD in rats. Excessive mechanical strain disrupted the CR and inhibited the expression of core clock proteins. The inhibitory effect of mechanical loading on the expression of extracellular matrix genes could be reversed by BMAL1 overexpression in NP cells. The Rho/ROCK pathway was demonstrated to mediate the effect of mechanical stimulation on CR. Prolonged mechanical loading for 12 months affected intrinsic CR genes and induced IDD in a model of upright posture in a normal environment. Unexpectedly, mechanical loading further accelerated the IDD in an Light-Dark (LD) cycle-disrupted environment. These results indicated that intrinsic CR disruption might be a mechanism involved in overloading-induced IDD and a potential drug target for night shift workers.
Emerging Roles of Krüppel-Like Factor 4 in Cancer and Cancer Stem Cells
Ding, Bo,Liu, Ping,Liu, Wen,Sun, Ping,Wang, Chun-Ling Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.9
Cancer stem cells (CSCs) are rare subpopulations within tumors which are recognized as culprits in cancer recurrence, drug resistance and metastasis. However, the molecular mechanisms of how CSCs are regulated remain elusive. Kr$\ddot{u}$ppel-like factors (KLFs) are evolutionarily conserved zinc finger-containing transcription factors with diverse functions in cell differentiation, proliferation, embryogenesis and pluripotency. Recent progress has highlighted the significance of KLFs, especially KLF4, in cancer and CSCs. Therefore, for better therapeutics of cancer disease, it is crucial to develop a deeper understanding of the mechanisms of how KLF4 regulate CSC functions. Herein we summarized the current understanding of the transcriptional regulation of K LF4 in CSCs, and discussed the functional implications of targeting CSCs for potential cancer therapeutics.
딩즈보(Zhi-Bo Ding),송승근(Seung-Keun Song) 한국콘텐츠학회 2018 한국콘텐츠학회논문지 Vol.18 No.11
본 연구는 해외 관광객들의 문화욕구를 분석하고 감천문화마을에서 제공할 수 있는 문화 서비스 요소를 조사하여 문화 서비스를 개선하는 구체적인 방책을 제시하고 궁극적으로 감천문화마을을 우수한 국제 관광 문화 브랜드를 육성하는 것을 목적으로 한다. 외국관광객들의 문화욕구가 무엇인지 알아보기 위해 도시 재생이 진행된 곳 가운데 감천문화마을을 대상으로 사례 연구를 수행하였다. 연구수행 기간은 4개월이었으며 6개국 외국관광객을 대상으로 문헌조사, 인터뷰, 참여관찰을 통해 기초자료를 수집하였다. 자료 분석은 도시재생, 문화욕구, 서비스 디자인 이론을 토대로 내용분석을 실시하였다. 본 연구는 감천문화마을의 5가지 문화 서비스 목표를 제시하였다. 첫째, 현지 특색문화의 전시 및 홍보, 둘째, 문화상품의 개발과 판매, 셋째, 좋은 관광이미지 부각, 넷째, 특이한 먹거리 제공, 다섯째, 문화체험 최적화이다. 본 연구를 통해 감천문화마을에 대한 문화서비스 향상에 도움을 줄 것으로 기대된다. The purpose of this study is to analyze the cultural needs of foreign tourists, to investigate the cultural service elements that can be provided in ‘Gamcheon Culture Village’, to suggest concrete policy to improve cultural services, and ultimately to cultivate excellent international tourism culture brands in ‘Gamcheon Culture Village’. In order to find out the cultural needs of foreign tourists, we conducted a case study on ‘Gamcheon Culture Village’ among the places where the urban regeneration proceeded. The study period was four months, and basic data were collected from foreign tourists from six countries through literature reviews, interviews and participation observation. Analysis of data was based on urban regeneration, cultural desire and service design theory, and we conducted content analysis. This study presented five cultural service goals of ‘Gamcheon Culture Village’. First, it displays and promotes local characteristic culture. Second, it develops and sells cultural products. Third, it brings up a good tourist image. Fourth, it provides an unusual food. Fifth, cultural experience optimization. Through this study, it is expected to help improve the cultural service of ‘Gamcheon Culture Village’.
Bo Zhang,Zhaoping Zhong,Kuan Ding,Lulu Yu 한국화학공학회 2014 Korean Journal of Chemical Engineering Vol.31 No.1
Element mercury (Hg0) from flue gas is difficult to remove because of its low solubility in water and high volatility. A new technology for photooxidative removal of Hg0 with an ultraviolet (UV)/H2O2 advanced oxidation process is studied in an efficient laboratory-scale bubble column reactor. Influence of several key operational parameters on Hg0 removal efficiency is investigated. The results show that an increase in the UV light power, H2O2 initial concentration or H2O2 solution volume will enhance Hg0 removal. The Hg0 removal is inhibited by an increase of the Hg0initial concentration. The solution initial pH and pH conditioning agent have a remarkable synergistic effect. The highest Hg0 removal efficiencies are achieved at the UV light power of 36W, H2O2 initial concentration of 0.125 mol/L, Hg0initial concentration of 25.3 μg/Nm3, solution initial pH of 5, H2O2 solution volume of 600 ml, respectively. In addition,the O2 percentage has little effect on the Hg0 removal efficiency. This study is beneficial for the potential practical application of Hg0 removal from coal-fired flue gas with UV/H2O2 advanced oxidation process.
The Incidence, Changes and Treatments of Cervical Deformity After Infection and Inflammation
Bo Han,Jianqiang Wang,Yong Hai,Duan Sun,Weishi Liang,Peng Yin,Hongtao Ding 대한척추신경외과학회 2023 Neurospine Vol.20 No.1
A healthy cervical spine with normal movement is the basis of many daily activities and is essential for maintaining a good quality of life. However, the alignment, fusion, and structure of the cervical spine can change for various reasons, leading to cervical deformity, mainly kyphosis. Approximately 5%‒20% of spinal infections in the cervical spine cause cervical deformity. The deformity can recover early; however, the disease's long-term existence or the continuous action of abnormal stress may lead to intervertebral fusion and abnormal osteophytes. Many gaps and controversies exist regarding infectious cervical deformities, including a lack of clear definitions and an acceptable classification system thereby requiring further research. Moreover, there is no consensus on the indications for postinfectious cervical deformity associated with Mycobacterium tuberculosis, Staphylococcus aureus, and Brucellosis. Therefore, we reviewed and discussed the incidence, clinical manifestations, changes, and treatment of infectious and inflammatory secondary cervical deformities from common to rare to provide a theoretical basis for clinical decision-making.
( Bo Xu ),( Li Ming Dai ),( Jun Jun Li ),( Meng Deng ),( Hua Biao Miao ),( Jun Pei Zhou ),( Yue Lin Mu ),( Qian Wu ),( Xiang Hua Tang ),( Yun Juan Yang ),( Jun Mei Ding ),( Nan Yu Han ),( Zun Xi Huang 한국미생물 · 생명공학회 2016 Journal of microbiology and biotechnology Vol.26 No.1
Xylanases sourced from different bacteria have significantly different enzymatic properties. Therefore, studying xylanases from different bacteria is important to their applications in different fields. A potential xylanase degradation gene in Massilia was recently discovered through genomic sequencing. However, its xylanase activity remains unexplored. This paper is the first to report a xylanase (XynRBM26) belonging to the glycosyl hydrolase family (GH10) from the genus Massilia. The gene encodes a 383-residue polypeptide (XynRBM26) with the highest identity of 62% with the endoxylanase from uncultured bacterium BLR13. The XynRBM26 expressed in Escherichia coli BL21 is a monomer with a molecular mass of 45.0 kDa. According to enzymatic characteristic analysis, pH 5.5 is the most appropriate for XynRBM26, which could maintain more than 90% activity between pH 5.0 and 8.0. Moreover, XynRBM26 is stable at 37°C and could maintain at least 96% activity after being placed at 37°C for 1 h. This paper is the first to report that GH10 xylanase in an animal gastrointestinal tract (GIT) has salt tolerance, which could maintain 86% activity in 5 M NaCl. Under the optimum conditions, Km, Vmax, and kcat of XynRBM26 to beechwood xylan are 9.49 mg/ml, 65.79 μmol/min/mg, and 47.34 /sec, respectively. Considering that XynRBM26 comes from an animal GIT, this xylanase has potential application in feedstuff. Moreover, XynRBM26 is applicable to high-salt food and seafood processing, as well as other high-salt environmental biotechnological fields, because of its high catalytic activity in high-concentration NaCl.
( Bo Xu ),( Fu Ya Yang ),( Cai Yun Xiong ),( Jun Jun Li ),( Xiang Hua Tang ),( Jun Pei Zhou ),( Zhen Rong Xie ),( Jun Mei Ding ),( Yun Juan Yang ),( Zun Xi Huang ) 한국미생물 · 생명공학회 2014 Journal of microbiology and biotechnology Vol.24 No.4
To isolate novel and useful microbial enzymes from uncultured gastrointestinal microorganisms, a fecal microbial metagenomic library of the pygmy loris was constructed. The library was screened for amylolytic activity, and 8 of 50,000 recombinant clones showed amylolytic activity. Subcloning and sequence analysis of a positive clone led to the identification a novel gene (amyPL) coding for α-amylase. AmyPL was expressed in Escherichia coli BL21 (DE3) and the purified AmyPL was enzymatically characterized. This study is the first to report the molecular and biochemical characterization of a novel α-amylase from a gastrointestinal metagenomic library.