Biofilm Formation and Cell Surface Properties of Staphylococcus aureus Isolates from Various Sources

Bo-Ram Kim,Young-Min Bae,Jin-Ha Hwang,이선영 한국식품과학회 2016 Food Science and Biotechnology Vol.25 No.2

This study investigated biofilm formation, cell surface hydrophobicity, colony spreading, and slime production for 112 Staphylococcus aureus strains isolated from various sources (leaf vegetables, pea leaf, perilla leaf, Kim-bab, person, and animal). When biofilm formation was classified by origin, S. aureus isolated from animal origin showed a significantly higher level of biofilm formation than others (p≤0.05). When S. aureus groups with different levels of biofilm formation (very strong, strong, moderate, and weak) were evaluated for the correlation with cell surface properties, there was a positive correlation between biofilm formation and hydrophobicity (r=0.926). Biofilm formation and colony spreading on tryptic soy broth (without dextrose) also showed positive correlation (r=0.863). In contrast, biofilm formation and slime production were negatively correlated (r= −0.973). Based on these results, the biofilm forming ability of S. aureus differs depending on their origin and might be affected by cell surface properties such as cell surface hydrophobicity.

S-580 : Vitamin D and carotid subclinical atherosclerosis in SLE

( Chang Bum Bae ),( Ju Yang Jung ),( Bo Ram Go ),( Hyun Ah Kim ),( Chang Hee Suh ) 대한내과학회 2013 대한내과학회 추계학술대회 Vol.2013 No.1

Vitamin D and carotid subclinical atherosclerosis in SLE Chang-Bum Bae, Ju-Yang Jung, Bo-ram Go, Hyun-Ah Kim, Chang-Hee Suh Department of Rheumatology, Ajou University School of Medicine, Suwon, Korea Introduction Atherosclerosis develops earlier in systemic lupus erythematosus (SLE) and is the important cause of mortality. It has been suggested that vitamin D deficiency contributed cardiovascular disease and immune dysregulation in SLE. Methods One-hundred two female patients with SLE and 52 normal controls (NC) were recruited. The carotid artery intima-media thickness (cIMT) and plague were assessed by B-mode ultrasound at the carotid artery level. Vitamin 25(OH)D3 levels were checked by immunoradiometric assay using the Bio-Line 25(OH)D3-Ria CT kit (Bio-Line S.A., Belgium). Disease activity markers were checked at the time of enrollment. Results The cIMT of SLE is 0.41±0.08mm, which is higher than NC (0.37±0.11 mm, p=0.012). Also, carotid artery plague index (PI) of SLE is higher than NC (0.68±1.39 vs 0.26±0.87, p=0.026). The cIMT was correlated with age (r=0.442, p<0.001), body mass index (r=0.246, p=0.013), waist-hip ratio (r=0.245, p=0.013), SLE disease activity index (r=0.239, p=0.016), taking aspirin (r=0.244, p=0.013), and the carotid artery PI was correlated with renal involvement (r=0.224, p=0.023) in patients with SLE. Vitamin 25(OH)D3 was not showed correlation with cIMT and carotid artery PI. Also, it was not correlated with disease activity markers. Conclusion In patients with SLE, the risk of cardiovascular disease measured by cIMT and carotid artery plaque was higher than NC, which may be derived from systemic inflammation. However, vitamin D levels were not showed significant correlations with carotid subclinical atherosclerosis. It may be not suitable to assess vitamin D as marker of disease activity or subclinical atherosclerosis.

서울 시내 일부 대학생의 성지식과 성태도에 관한 연구

김보배,노은경,박서영,박영,오승희,전영란,차현진,윤희상,김수지,이자형 이화여자대학교 간호과학대학 2004 이화간호학회지 Vol.- No.38

This study surveyed and analyzed comparatively college students-knowledge' attitude, was to cultivate the right knowledge and attitude on sex, and serve the foundational data for the suitable sex education. The purpose of the study was to identify and examine: 1) students; sexual knowledge and sexual attitude; 2) the differences between variables of gender, age, and self-perceived sexual knowledge in relation to sexual knowledge and sexual attitude; and 3) the relationship between sexual knowledge and sexual attitude. For this study the questionaires were given to 300 students from five different universities located seoul areas. the questionaires were collected Jenuary 8th to 15th in 2004. Two hundred and nigty one samples were analyzed for the study. The adapted SKAT(saxual knowledge & attitude test; Lief & Reed, 1986) was used to determine sexual knowledge and the students' sexual attitude. Collected data were analyzed statistically by means of percentage, t-test, ANOVA test, and pearson correlation analysis with SPSS 12.0 package program. The main results of this analysis are as follows: Participants consisted of male(48.1%) and female(51.9%) which were mainly between the ages of 21.25(71.8%) with an age range of 19 to 30. Knowledge of sexual disease was scored highest of 92.4%, and konwledge of the knowledge of the male and female's anatomy was the lowest at 60.9%. The respondents' level of sexual attitude was tolerant as on average 3.66(on the basis of 5) Relating to the items, the respondents' score of the sexual curiosity was 4.16, sexual behavior 3.85, self-consolation 3.81, aborthon 3.23, virgine 3.19, extramarital experiences 3.17. There were no significant differences in sexual knowledge between male and females, between younger and older students, residence, sexual experience, and how students mainly obtained their sexual knowledge. But a significant difference was also found religion in their sexual attitude(p<.005). In addition, results showed the relationship between sexual knowledge and sexual attitude(P=0.332).

편측성 만성 부비동염의 해부학적 유발 인자

김영배,최광석,강보승,김남표,김종애 침례병원 1998 浸禮病院學術誌 Vol.14 No.-

연취급 근로자들의 사회 심리적 스트레스와 연노출 지표들간의 관련성

김용배,안현철,황보영,리갑수,황규윤,이성수,안규동,이병국 大韓産業醫學會 2000 대한직업환경의학회지 Vol.12 No.1

목 적 : 연취급 근로자를 대상으로 사회 심리적 스트레스 수준을 알아보고 이를 기초자료로 활용하며 사회 심리적 스트레스와 연노출 지표들간의 관련성을 알아보고자 시도하였다. 방 법 ' 1차 연제련업종의 근로자 234명을 대상으로 일반적 특성, 사회 심리적 스트레스, 직업적 특성과 사회적 지지를 문진과 설문지를 통해 확인했으며 연노출 수준을 알기 위해 혈중 ZPP 농도, 혈중연농도, 요중 δ-ALA 배설량, 혈색소, 혈구용적치등을 측정했다. 결 과 : 단일변량 분석결과 혈중 연농도 수준과 직업적 특성에 따른 사회 심리적 스트레스는 유의한 차이가 있었으며 혈중 연농도가 증가함에 따라 스트레스 수준도 증가되는 경향을 나타냈다. 사회 심리적 스트레스와 연노출 지표 및 다른 변수들간의 관련성을 파악하기 위하여 상관분석을 실시한 결과 혈중 연농도만이 사회 심리적 스트레스와 양의 상관관계를 나타냈으며 유의했다. 결 론 : 고농도 연노출의 가능성이 있는 직종인 경우 기존의 연관련 자각증상에 대한 설문조사와 함께 사회 심리적 스트레스 측정도 병행하여 실시하는 것이 연에 의한 건강장해를 조기에 발견하고 예방하는데 필요하다. Objectives : This study was carried out to assess psychosocial distress of lead workers and to examine the relationship between lead exposure indices and psychosocial distress. Methods The study design was cross-sectional and research subjects were 234 lead workers in primary smelting industry. General characteristics, job characteristics and social support were obtained by self-administered questionnaire and history taking. Psychosocial distress was measured using PWI(Psychosocial Well-being Index), a 45-item self-administered instrument. Blood lead(PbB), zinc protoporphyrin(ZPP), δ-aminolevulinic acid in urine (ALAU) were selected as indicators of lead exposure. Results : There was significant difference in PWI according to lead exposure level and job characteristics. The results of correlation analysis showed that PWI was significantly correlated with PbB (r=0.203, p=0.002). Conclusions : Our results supported the association between lead exposure and psy-chosocial distress. The inclusion of psychosoclal distress measurement as a special health examination for lead workers was highly recommended for the effective health manage-ment.

연폭로 근로자들에서 빈혈증 관련 인자에 관한 연구

황보영,김용배,김화성,이성수,안규동,이병국 순천향대학교 교수학습개발센터 2004 Journal of Soonchunhyang Medical Science Vol.10 No.3

Objectives : This study intended to obtain the interrelationship of lead exposure indices and anemia related factors for the better health management of lead exposed workers. Methods : 502 male workers in storage battery industries as lead exposed group and 106 male office workers as contorl group were selected for this study. The examined variables wer age, work duration, smoking and alcohol crinking habit, blood zinc protoporphyin concentraion (ZPP), blood lead concentration (PbB), urinary δ-aminoevulinic acid (ALAU), hemoglobin (Hb), hematocrit (Hct), serum calcium (S-Ca), serum iron (S-Fe), total iron binding capacity (TIBC), and percentage trasferrin saturation (PTS). Results : The means of ZPP, PbB ALAU in lead exposed proup were 58.4±35.7 ㎍/㎗, 27.6±10.9 ㎍/㎗, and 1.61±2.00 mg/ℓ, respectively. These three values in lead exposed group were significantly higher than control group (P<.05). The means of Hb, Hct, S-Ca, S-Fe, TIBC, PTS in lead exposed group were 15.3±1.1 g/㎗, 47.2±4.5 %, 9.4±0.4 ㎍/㎗, 124.0±67.5 ㎍/㎗, 317.1±68.5 ㎍/㎗, and 38.7±15.2 %, respectively. Hb, Hct, S-Ca, S-Fe, and TIBC in lead exposed group were significantly lower than contrl group (P<.05). PTS was no significant difference in lead exposed group and control group (P> .05). Hct, S-Ca, S-Fe, PTS were significant positive correlation with Hb and ZPP, corrected PbB, AlAU were significant negaitive correlation with Hb in lead exposed group (P< .05). On multiple regression using ZPP as a dependent variable and PbB, S-Ca and S-Fe as independent variables after adjustment for possible confounder such as age, smoking and drinking habit, Pbb, S-Ca, S-Fe and age contributed to ZPP. Conclusions : As the result of this study, it was suggested that inclusion of S-Ca, S-Fe, PTS measurement as a anemia screening test for lead workers was highly recommended for the effective prevention and early diagnosis of anemia.

렙토스피라증의 진단에 있어 수동혈구응집반응법과 육안응집반응법의 정확도에 관한 연구

최보율,김영규,이수진,송재철,박항배 한양대학교 의과대학 1994 한양의대 학술지 Vol.14 No.2

Before 1984, Leptospirosis had been reported sporadically in middle inland of Korea as hemorrhagic pneumonia like disease, epidemic pulmonary hemorrhagic fever or epidemic pneumonitis since 1975. The epidemiologic characteristics of this disease were found out and the agent was isolated microbiologically after a large epidemic in 1984. Thereafter many consecutive studies for leptospirosis had been performed in the realm of epidemiology, microbiology and clinical field. But in order to appropriate control of Leptospirosis there are many problems such as organizing surveillance system for early diagnosis and treatment, identifying sero-group and serovar in each epidemic area and assessing vaccine efficacy. The objectives of this study was to find out easily applicable diagnostic methods to be used in a rural community surveillance system. Two diagnostic methods, passive hemagglutination(PHA) test and macroscopic agglutination test(maAT) were measured against their accuracies, sensitivity, specificity and predictive value of positive and negative. 301 sample sera from 270 human cases was collected through Leptospirossis surveillance system in a rural community during 1989-1992. Microscopic agglutination test(MAT) were used for confirmed diagnosis according to WHO guideline with 4 strains; Icterohemorrhagiae lai and Canicola canicola Hond Utrecht IV as standard strians and 6P-014-1 and YP-35-1 as strains isolated in Korea in 1986. The results are as follows. 1. 75(24.9%) among 301 sample sera and 49(18.1%) among 270 persons was serologically positive with MAT. 2. Agreement between PHA and MaAT was very low(k-coefficient≤0.252). 3. In PHA, specificity was very high(≥0.96) but sensitivity was very low(≤0.28). 4. In MaAT, specificity(≥0.73) was relatively lower than PHA but sensitivity (≥0.79) was significantly higher than PHA, especially false-positive rate case was absent in MAT's high antibody titer(above 1:640). It was concluded that MaAT method was a very useful diagnostic tool for early diagnosis of Leptospirosis in a rural community surveillance system, provided that confirmed diagnosis with MAT method be carried out in laboratory of medical school or research institute such as National Institute of Health or Provicial government Institute of Health and Environment.

탄소섬유의 CVD-SiC 증착특성에 미치는 CH₃SiCl₃대 H₂의 비율의 영향

박병배,심환보,이보성,이영석,김종환 忠南大學校 産業技術硏究所 1994 산업기술연구논문집 Vol.9 No.2

Chemical vapor deposition of SiC on carbon fibers was investigated to improve oxidation resistance of carbon fibers, which was performed at the horizontal hot-wall reactor using the reactant mixtures of CH₃SiCl₃and H₂ with carrier gas Ar. The chemical composition, oxidation resistance, and tensile strength of SiC-coated carbon fiber were investigated as functions of CH₃SiCl₃/H₂ ratio, reaction temperature, coating thickness, and so on. The composition of deposited layer revealed C rich β-SiC at low hydrogen concentration and Si rich β-SiC at high hydrogen concentration. Because Si rich β-SiC layer is easy to be converted into SiO₂ favorable to oxidation resistance, high input value of H₂/CH₃SiCl₃ mole ratio resulted in good oxidation resistance in the SiC coating layer of carbon fibers.

자궁내막증 백서모형에서 Danazol과 Decapeptyl의 치료효과에 관한 연구

황선보,이민석,심재현,김동훈,배한익 동국대학교 경주대학 1996 東國論集 Vol.15 No.-

본 연구의 목적은 장간막에 자궁내막 조직을 자가이식하여 수술적으로 유도된 자궁내막증 백서모형에서 자궁내막증의 치료를 위해 danazol과 decapetyl의 치료효과를 평가하는 것이다. 백서(n=48)들은 무작위적으로 정상대조군(n=12)과 수술적으로 유도된 자궁내막증군(n=36)으로 구분하였다. 후자는 비치료군과, danazol 치료군과 decapeptyl 치료군으로 세분하였다(각 군의 n=12). Danazol(8mg/일)은 수술후 4째주부터 구강을 통해 투여되었다. Decapeptyl(0.2mg/2주)은 근주하였다. 처음투약후 10일, 20일, 40일, 60일째에 각군의 2마리의 쥐를 희생시켰다. 저자들은 자궁내막증 이식체와 난소의 크기를 측정하여 다음의 결론를 얻었다. 1. 자궁내막증 이식체의 크기는 비치료군에 비해(3.91±1.08mm) danazol 치료군과 decapeptyl치료군에서 (각각2.28±0.17mm 과 2.78±0.97mm), 현저히 감소되었다(p<0.01). 2. danazol과 decapeptyl 두 치료군에서 장기치료가 단기치료보다 자궁내막증 이식체의 크기를 감소시키는데 더 효과적이었다(p<0.01). 3. 난소의 크기는 정상대조군과, 비치료군과 decapeptyl 치료군에서 유의하게 차이를 보이지 않은데 반해(각각 4.46±0.85mm, 4.77±0.82mm과 4.97±0.92mm), danazol 치료군에서는 유의하게 감소하였다(3.97±0.63mm) (p<0.63mm). 백서모형에서 수술적으로 이식된 자궁내막조직의 성장과 발달은 자궁내막증의 병인 이해와 신약개발에 유용한 연구모델을 제공할 수 있을 것 이다. The purpose of this study was to surgically induce endometriosis in the rat by autotransplanting endometrial tissue to the mesentery and to evaluate the efficacy of danazol and decapeptyl for the treatment of endometriosis. Rats(n=48) were randomly assigned to normal control group(n=12) and surgically induced endometriosis group(n=36). The latter was further divided into the untreated, danazol treated and decapeptyl treated groups (n=12 for each group). Danazol(8mg/day) was orally administrated from 4weeks after operation. Decapeptyl(O.2ml/2weeks) was injected intramuscularly. Two rats of each group are sacrificed on the 10th, 20th, 40th, 60th, 80th and lOOth day after the first drug administration. We measured sizes of endometriotic implants and ovaries. The results were as follows; 1. The sizes of endometriotic implants were significantly decreased(P<O.Ol) in the danazol and decapeptyl treated groups (2.28±O.71mm and 2.78±O.89mm, respectively) compared with untreated group(3.91±l.08mm). 2. Long term treatment was more effective than short term treatment in decreasing the size of endometriotic implants in both danazol and decapeptyl treated groups(P<O.Ol). 3. The size of ovaries was not significantly different among the normal control, untreated and decapeptyl treated groups(4.65±O.83mm, 4.77±O.82mm and 4.97±O.92mm, respectively) but was significantly decreased(P<O.Ol) in the danazol treated group(3.97±O.63mm). The successful growth and development of surgically transplanted endometrial tissue in the rat may offer an useful research model that can be used to understand pathogenesis of endometriosis and to explore new drug therapies.

NOD/SCID 마우스 모델을 이용한 인간 제대혈 혼합이식에서의 생착 양상 분석

정양조,김동욱,조빈,강영주,박보배,김혜정,김태규,오일환 대한조혈모세포이식학회 2002 대한조혈모세포이식학회지 Vol.7 No.2

연구배경: 제대혈은 골수에 비해 많은 장점을 가지고 있음에도, 이식 후 성적에 있어 총세포수에 의해 좌우되며, 한 개의 태반으로부터 얻을 수 있는 총세포수의 한계가 있어 주로 소아에 국한되고 있다. 따라서 제대혈 응용의 확대를 위해 생착량을 늘이기 위한 방법으로 다중공여자에 의한 제대혈 혼합이식을 늘일 수 있는지 연구하기 위하여, 본 연구에서는 주조직적합성 형별로 조합된 제대혈을 NOD/ SCID 생쥐에 이식하는 방법으로, 혼합생착의 가능성을 연구하였다. 방법: 조합된 2개 공여자로부터 얻어진 제대혈들을 단일이식 및 혼합이식하여, 혼합이식된 숙주에 생착된 세포를 PCR-SSOP 방법을 사용하여 공여자 별로 추적하였다. 또한 각 공여자세포의 상대적 생착비율을 대조군인 단일제대혈 이식에서 얻어진 생착량과 비교하는 정량적 비교도 함께 하였다. 결과: 총단핵구를 혼합이식한 실험군에서는 주조직적합성의 일치도에 관계없이 한쪽공여자의 세포가 다른쪽에 비해 우세한 편향생착을 보였다. 그러나 임파구를 비롯한 Lineage 양성세포를 제거한 실험군에서는 주조직적합성의 불일치에도 불구하고 뚜렸한 생착의 공존이 관찰되었다. 결론: 본 연구는 제대혈의 생착량을 증가를 목적으로 총단핵구를 혼합이식할 경우 한쪽공여자의 세포에 의해 편중되며, 이것을 이식 후 생착과정에서의 두 종류의 세포의 경쟁에 의해 초래된다는 것을 보이고 있다. 또한 이러한 경쟁은 제대혈에 포함되어 있던 임파구들에 의한 것이며, 생착공존을 위하여는 공여자간 주조직적합성의 일치도 보다, 임파구의 제거가 더 중요하다는 것을 보여주고 있다. Background: Although umbilical cord blood (UCB) has been an attractive alternatives for bone marrow cells, application is limited to pediatric case due to limitation in total number of nucleated cells, that is a single most important factor for outcomes of UCB transplantation. Therefore, increasing overall engraftment by mixed transplantation of UCB derived from multiple donor should comprise one strategy to circumvent the posed limitation of UCB transplantation. In order to investigate the feasibility of establishing co-engraftment by multi-donor UCB cells, we carried out a xenotransaplantation study using NOD/SCID mice for systemic analysis of results on the input-based control of single unit transplantation. Methods: UCB units with various extent of HLA-matchings were co-transplanted into NOD/SCID mice along with single unit transplantation control. The relative contributions of engraftment by cells from each donor-derived were analyzed by HLA polymorphism using PCR-SSOP. Results: In all HLA-based mixed transplantation of total nucleated UCB cells, engraftment of one donor predominated over the other despite that equivalent amount of engraftments were achieved by single donor transplantation. When lineage depleted UCB units were co-transplanted, significant degree of co-engraftments were observed regardless of HLA disparity. Conclusion: Our result show that one donor cells dominate over the other in mixed UCB transplantation and that it is due to competition between donor cells during post-transplantation process. Our results suggest that immune cells contained in UCB unit mediate such competitioin and that 6 locus HLA matching would not be sufficient to prevent the competition.