Quality evaluation of Perillae Folium by HPLC/PDA

Bing Tian Zhao,이강노,이제현,민병선,손종근,우미희 대한약학회 2015 Archives of Pharmacal Research Vol.38 No.8

To establish a standard of quality control for Perillae Folium (Lamiaceae Family), four standard compounds including rosmarinic acid, elemicin, perillaldehyde, and dillapiole were evaluated by high-performance liquid chromatography (HPLC)/photodiode array (PDA). The four standards were analyzed with a Phenomenex Kinetex C18 (250 9 4.6 mm, 5 lm) column by gradient elution using 0.1 % formic acid in water and methanol as the mobile phase. The standards were quantified by HPLC/ PDA from Perillae Folium, which included the leaf and twig of Perilla frutescens L. Britton var. acuta (Thunb.) Kudo or Perilla frutescens Britton var. crispa Decne. The method was successfully used in the analysis of Perillae Folium, and the linearity, recovery, precision, accuracy, stability, and robustness were satisfactory according to the validation results. In Perillae Folium samples, the average contents (wt%) of rosmarinic acid, elemicin, perillaldehyde, and dillapiole were 0.540, 0.059, 0.050, and 0.056 %, respectively. The results indicate that the establishedHPLC/PDA method is suitable for the quantitation and quality evaluation of Perillae Folium.

Quantitative determination and pattern recognition analyses of bioactive marker compounds from Dipsaci Radix by HPLC

Bing Tian Zhao,Su Yang Jeong,문동철,손건호,손종근,우미희 대한약학회 2013 Archives of Pharmacal Research Vol.36 No.11

In this study, quantitative and pattern recognitionanalyses were developed using HPLC/UV for thequality evaluation of Dipsaci Radix. For quantitativeanalysis, five major bioactive compounds were assessed. The separation conditions employed for HPLC/UV wereoptimized using ODS C18 column (250 9 4.6 mm, 5 lm)with a gradient of acetonitrile and water as the mobilephase at a flow rate of 1.0 mL/min and a detection wavelengthof 212 nm. These methods were fully validated withrespect to linearity, accuracy, precision, recovery, androbustness. The HPLC/UV method was applied successfullyto the quantification of five major compounds in theextract of Dipsaci Radix. The HPLC analytical method forpattern recognition analysis was validated by repeatedanalysis of 17 Dipsaci Radix and four Phlomidis Radixsamples. The results indicate that the established HPLC/UV method is suitable for quantitative analysis.

High Performance Liquid Chromatography used for Quality Control of Achyranthis Radix

Bing Tian Zhao,우미희,Su Yang Jeong,문동철,손건호,손종근 대한약학회 2012 Archives of Pharmacal Research Vol.35 No.8

To establish a standard of quality control and to identify reliable Achyranthis Radix, three phytoecdysones including ecdysterone (1), 25R-inokosterone (2) and 25S-inokosterone (3) were determined by quantitative HPLC/UV analysis. Three phytoecdysones were separated with an YMC J’sphere ODS C18 column (250 mm × 4.6 mm, 4 μm) by isocratic elution using 0.1% formic acid in water and acetonitrile (85:15, v/v%) as the mobile phase. The flow rate was 1.0 mL/min and the UV detector wavelength was set at 245 nm. The standards were quantified by HPLC/UV from Achyranthes bidentata Blume and Achyranthes japonica Nakai, as well as Cyathula capitata Moq. and Cyathula officinalis Kuan, which are of a different genus but are comparative herbs. The method was successfully used in the analysis of Achyranthis Radix of different geographical origin or genera with relatively simple conditions and procedures, and the assay results were satisfactory for linearity, recovery, precision, accuracy, stability and robustness. The HPLC analytical method for pattern recognition analysis was validated by repeated analysis of eighteen A. bidentata Blume samples and ten A. japonica Nakai samples. The results indicate that the established HPLC/UV method is suitable for quantitation and pattern recognition analyses for quality evaluation of Achyranthis Radix.

Protein tyrosine phosphatase 1B inhibitors from natural sources

Bing Tian Zhao,Duc Hung Nguyen,Duc Dat Le,최재수,민병선,우미희 대한약학회 2018 Archives of Pharmacal Research Vol.41 No.2

Since PTP1B enzyme was discovered in 1988, ithas captured the research community’s attention. Thislandmark discovery has stimulated numerous researchstudies on a variety of human diseases, including cancer,inflammation, and diabetes. Tremendous progress has beenmade in finding PTP1B inhibitors and exploring PTP1Bregulatory mechanisms. This review investigates for thenatural PTP1B inhibitors, and focuses on the commoncharacteristics of the discovered structures and structure–activity relationships. To facilitate understanding, all thenatural compounds are here divided into five differentclasses (fatty acids, phenolics, terpenoids, steroids, andalkaloids), according to their skeletons. These PTP1Binhibitors of scaffold structures could serve as a theoreticalbasis for new concept drug discovery and design.

Simultaneous quantitation and validation of method for the quality evaluation of Eucommiae cortex by HPLC/UV

( Bing Tian Zhao ),( Su Yang Jeong ),( Tae In Kim ),( Eun Kyoung Seo ),( Byung Sun Min ),( Jong Keun Son ),( Mi Hee Woo ) 영남대학교 약품개발연구소 2016 영남대학교 약품개발연구소 연구업적집 Vol.26 No.-

A new HPLC/UV method has been developed for the simultaneous quantitative determination of four major components in Eucommiae cortex, namely geniposidic acid (1), geniposide (2), pinoresinol di-O-β-D-glucopyranoside (3), and liriodendrin (4). Simultaneous separations of these four components were achieved on a J``sphere ODS CI8 column (250 x 4.6 mm, 4 mm), The elution was done using water with 0.1 % phosphoric acid (A) and acetonitrile with 0.1 % phosphoric acid (B) in a two-step elution of the mobile phase at a flow rate of 1.0 mL/min and a wavelength of 230 nm. The method was validated for linearity, recovery, precision, accuracy, stability and robustness. All calibration curves showed good linear regression (r² > 0.999) within the test ranges. This method showed good recovery and repro-ducibility for the quantification of these four components in 85 species of Eucommiae cortex. The intra-day and inter-day precisions were lower than 0.53 % (as a relative standard deviation, RSD) and accuracies between 93.00 and 106.28 % for all standards. The results indicate that the established HPLC/UV method is suitable for quantitation and quality evaluation of Eucommiae cortex.

Quality evaluation and pattern recognition analyses of marker compounds from five medicinal drugs of Rutaceae family by HPLC/PDA

Bing Tian Zhao,김은정,손건호,손종근,민병선,우미희 대한약학회 2015 Archives of Pharmacal Research Vol.38 No.8

To establish a standard of quality control and to identify different origins for the Rutaceae family [Citri Unshiu Peel (CU), Citri Unshiu Immature Peel (CI), Ponciri Immature Fructus (PI), Aurantii Immature Fructus (AI), and Aurantii Fructus (AU)], 13 standards including rutin (1), narirutin (2), naringin (3), hesperidin (4), neohesperidin (5), neoponcirin (6), poncirin (7), naringenin (8), isosinensetin (9), sinensetin (10), nobiletin (11), heptamethoxyflavone (12), and tangeretin (13) were determined by high performance liquid chromatography (HPLC)/photo-diode array (PDA) analysis. A YMC ODS C18 (250 9 4.6 mm, 5 lm) column was used and the ratio of mobile phases of water (A) and acetonitrile (B) delivered to the column for gradient elution was applied. This method was fully validated with respect to linearity, accuracy, precision, stability, and robustness. The HPLC/PDA method was applied successfully to quantify 13 major compounds in the extracts of CU, CI, PI, AI, and AU. The pattern recognition analysis combinedwith LC chromatographic data was performed by repeated analysis of 27 reference samples in the above five Rutaceae orientalmedicinaldrugs.TheestablishedHPLCmethodwas rapid and reliable for quantitative analysis and quality control of multiple components in five Rutaceae species with different origins.

Cytotoxic and Anti-oxidant Constituents from the Aerial Parts of Aruncus dioicus var. kamtschaticus

Bing Tian Zhao,김영호,우미희,Su Yang Jeong,민병선,Viet Dung Vu 한국생약학회 2013 Natural Product Sciences Vol.19 No.1

Ten compounds (1 - 10), palmitic acid (1), 10-nonacosanol (2), pentacosan-1-ol (3), phytol (4), b-sitosterol (5), b-sitosterol-3-O-b-D-glucopyranoside (6), 2,4-dihydroxycinnamic acid (7), hyperoside (8), uridine (9) and adenosine (10), were isolated from the n-hexane and EtOAc-soluble fractions of the aerial parts of A. dioicus var. kamtschaticus (Rosaceae). The structures of these compounds were elucidated on the basis of spectroscopic evidence. All compounds (1 - 10) were isolated for the first time from this plant. Cytotoxicity of 1 - 10 against Jurkat T (T-lymphocytic leukemia cells), HeLa (Human cervical epitheloid carcinoma cells), MCF-7 (Human breast cancer cells), and HL-60 (Human promyelocytic leukemia cells) cell lines was measured. Compound 6 showed good cytotoxicity against HL-60 cell line with IC50 value of 8.13 mg/mL. In addition, compounds 7 and 8 exhibited antioxidant activity with IC50 values of 16.30 and 12.42 mg/mL, respectively.

Discrimination between steam processed and unprocessed Tubers of Gastrodia elata Blume by HPLC

Bing Tian Zhao,Si Whan Song,Duc Dat Le,Eunsook Ma,Jong-Keun Son,Mi Hee Woo 한국분석과학회 2019 분석과학 Vol.32 No.6

In this study, to evaluate the effectiveness and safety of oral therapy using Gastrodiae Rhizoma, a new HPLC-PDA analysis method was developed for the simultaneous quantitation of the three major components: (1) gastrodin, (2) gastrodigenin, and (3) p-hydroxybenzaldehyde, in steam processed and unprocessed tubers of Gastrodia elata Blume. The clear separation of the three components was achieved on a C18 column (250 × 4.6 mm, 5 μm) by gradient elution using water (including 0.1 % formic acid) and acetonitrile as the mobile phase. The flow rate was 1.0 mL/min, and the UV detector wavelength was set at 270 nm. The results demonstrate satisfactory linearity, recovery, precision, accuracy, stability, and robustness. The established HPLC-PDA method was applied to quantify three major compounds in 59 samples of G. elata Blume tubers. Finally, the steam processed and unprocessed tubers of G. elata Blume were successfully distinguished by pattern recognition analysis.

Quantitative analysis of betaine in Lycii Fructus by HILIC-ELSD

Bing Tian Zhao,Su Yang Jeong,Kyoung Hwangbo,문동철,서은경,이동호,이제현,민병선,마은숙,손종근,우미희 대한약학회 2013 Archives of Pharmacal Research Vol.36 No.10

A rapid and simple high-performance liquidchromatography (HPLC) method with evaporative lightscattering detection (ELSD) was developed for the determinationof betaine from Lycii Fructus. Betaine was separatedwith an Atlantis hydrophilic interaction liquidchromatography silica column (4.6 9 150 mm, 5 lm,100 A ° ) by isocratic elution using 30 mM ammonium acetatebuffer and acetonitrile (20:80, v/v %) as the mobilephase. The flow rate was 1.0 mL/min, and the temperaturefor the spray chamber and drift tube was set at 30 and50 C, respectively. The method was fully validated withrespect to linearity, precision, accuracy, stability androbustness. The HPLC/ELSD method was applied successfullyto the quantification of betaine in the extract ofLycii Fructus. The HPLC analytical method for patternrecognition analysis was validated by repeated analysis oftwenty-six L. barbarum L. from China (BC01–BC26), 3 L. barbarum L. (BJ27–BJ29) from Japan, 12 L. chinenseMiller from China (CC30–CC41) and 51 L. chinense Millersamples (CK42–CK92) from Korea. The results indicatethat the established HPLC/ELSD method is suitable forquality evaluation of Lycii Fructus.

RESEARCH ARTICLE : Quantitative analysis of betaine in Lycii Fructus by HILIC-ELSD

( Bing Tian Zhao ),( Su Yang Jeong ),( Kyoung Hwangbo ),( Dong Cheul Moon ),( Eun Kyoung Seo ),( Dongho Lee ),( Je Hyun Lee ),( Byung Sun Min ),( Eun Sook Ma ),( Jong Keun Son ),( Mi Hee Woo ) 영남대학교 약품개발연구소 2014 영남대학교 약품개발연구소 연구업적집 Vol.24 No.0

A rapid and simple high-performance liquid chromatography (HPLC) method with evaporative light scattering detection (ELSD) was developed for the determination of betaine from Lycii Fructus. Betaine was separated with an Atlantis hydrophilic interaction liquid chromatography silica column (4.6 × 150 mm, 5 μm, 100 A) by isocratic elution using 30 mM ammonium acetate buffer and acetonitrile (20:80, v/v %) as the mobile phase. The flow rate was 1.0 mL/min, and the temperature for the spray chamber and drift tube was set at 30 and 50 °C, respectively. The method was fully validated with respect to linearity, precision, accuracy, stability and robustness. The HPLC/ELSD method was applied successfully to the quantification of betaine in the extract of Lycii Fructus. The HPLC analytical method for pattern recognition analysis was validated by repeated analysis of twenty-six L. barbarum L. from China (BC01-BC26), 3 L. barbarum L. (BJ27-BJ29) from Japan, 12 L. chinense Miller from China (CC30-CC41) and 51 L. chinense Miller samples (CK42-CK92) from Korea. The results indicate that the established HPLC/ELSD method is suitable for quality evaluation of Lycii Fructus.