<i>Lycopus lucidus</i> Turcz Inhibits the Osteoclastogenesis in RAW 264.7 Cells and Bone Loss in Ovariectomized Rat Model

Jeong, Da-Won,Kim, Eun-Young,Kim, Jae-Hyun,Lee, Bina,Hong, SooYeon,Park, Jae Ho,Jung, Hyuk-Sang,Sohn, Youngjoo Hindawi 2019 Evidence-based Complementary and Alternative Medic Vol.2019 No.-

<P><I>Lycopus lucidus</I> (LL) is a perennial herb that is traditionally used in Asia to treat edema, wound healing, and gynecological diseases such as irregular menstruation and menstrual pain. We hypothesized that LL would decrease the risk of developing osteoporosis, which is a condition related to gynecological diseases. In this study, we aimed to investigate the effect of a water extract of LL on osteoclastogenesis<I> in vitro</I> and osteoporosis<I> in vivo</I>.<I> In vitro</I> study, we used RAW 264.7 cells as osteoclast precursor cell. Osteoclast differentiation was induced by receptor activator nuclear factor-kappa B ligand (RANKL). We investigated the effect of LL on RANKL-induced osteoclastogenesis, tartrate-resistant acid phosphatase (TRAP) activity, and osteoclast-related genes.<I> In vivo</I> study, we used ovariectomized- (OVX-) induced osteoporosis rat model. OVX-induced Sprague-Dawley rats were randomly separated into sham, OVX, 17<I>β</I>-estradiol (100 <I>μ</I>g/kg), wLL-L (15.2 mg/kg), and wLL-H (152 mg/kg) groups. Drugs were administered orally once daily for 9 weeks. wLL inhibited the formation of TRAP-positive osteoclasts; TRAP activity; pit formation; transcription factors (the nuclear factor of activated T-cell cytoplasmic 1 and c-fos); and osteoclast-related genes such as TRAP, carbonic anhydrase II, cathepsin K, osteoclast-associated receptor, and the d2 isoform of the vacuolar ATPase Vo domain. Also, wLL prevented loss of the trabecular area in the OVX femur without change of estrogen level. These results indicate that wLL is able to inhibit osteoclastogenesis and protect bone loss in the OVX-induced osteoporosis model without the influence of hormones like estrogen.</P>

Effect of peiminine on DNCB-induced atopic dermatitis by inhibiting inflammatory cytokine expression <i>in vivo</i> and <i>in vitro</i>

Lim, Jeong-Min,Lee, Bina,Min, Ju-Hee,Kim, Eun-Young,Kim, Jae-Hyun,Hong, SooYeon,Kim, Jwa-Jin,Sohn, Youngjoo,Jung, Hyuk-Sang Elsevier 2018 INTERNATIONAL IMMUNOPHARMACOLOGY Vol.56 No.-

<P><B>Abstract</B></P> <P>Peiminine (PMN) is the main component derived from Fritillaria ussuriensis and is used in traditional medicine in East Asia. The aim of this study was to evaluate the effects of PMN on atopic dermatitis (AD) induced by a dinitrochlorobenzene (DNCB) in Balb/c mice. Inflammatory cytokine expression of PMN was investigated <I>in vitro</I>. Eosinophil infiltration and the thickness of DNCB-induced AD mouse skin were measured. The levels of IgE, IL-4, IL-6, IL-13, and TNF-α in the serum were measured by ELISA. The effects of PMN on the transcription level of MAPK and nuclear factor (NF)-κB were evaluated in mouse skin. In addition, the inhibitory effect of TNF-α, IL-1β, COX-2 and PGE2 were measured in RAW264.7 cells; TARC was investigated in HaCaT cells; and β-hexosaminidase was examined in RBL-2H3 cells. PMN decreased the number of eosinophils in the dermis as well as mast cells and decreased the thickness of the epidermis and dermis. The PMN High group had a significantly reduced serum level of IgE, IL-4, IL-13 and TNF-α. Moreover, P-ERK and P-P38 were inhibited in the PMN High group compared with the DNCB-treated group. PMN additionally attenuated the expression of inflammatory cytokines in cells, including RAW264.7, HaCaT and RBL-2H3 cells. Our results suggest that PMN could be a potential therapeutic candidate for the treatment of AD.</P> <P><B>Highlights</B></P> <P> <UL> <LI> PMN has an inhibitory effect on atopic dermatitis by decreasing inflammatory cytokines. </LI> <LI> PMN reduced the number of eosinophils and mast cells as well as MAPK and NF-κB signaling in tissues. </LI> <LI> PMN decreased COX-2 and prostaglandin E2 in RAW264.7. </LI> <LI> PMN inhibited TARC in human keratinocytes and reduced β-hexosaminidase in RBL-2H3 cells. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

柴胡가 OVA로 유발된 BALB/c mice의 알레르기성 비염 모델에 미치는 영향

임정민(Jeong-Min Lim),허준(Jun Heo),이성엽(SungYub Lee),김재현(Jae-Hyun Kim),이비나(Bina Lee),민주희(Ju-Hee Min),김은영(Eun-Young Kim),정혁상(Hyuk-Sang Jung),손영주(Youngjoo Sohn) 한의병리학회 2015 동의생리병리학회지 Vol.29 No.6

Analysis of SSEA1+ vs. SSEA1- fractions of bulk-cultured XENP cell lines

Minjin Jeong,Kyeng-Won Choi,김승준,김정호,Bert Binas 한국바이오칩학회 2012 BioChip Journal Vol.6 No.1

Previously isolated rat extraembryonic endoderm precursor (XENP) cell lines had been characterized after clonal density plating. The arising colonies had consisted of peripheral XENP cells expressing the surface antigen SSEA1 and the transcription factor Oct4, and inner XENP-derived extraembryonic endoderm cells that were nearly negative for SSEA1 and Oct4. We now sorted bulk-cultured XENP cell lines from two rat strains by FACS into SSEA1+ and SSEA1- populations and compared their expression profiles by microarray and RT-PCR. In the bulk cultures, the SSEA1+ fraction was only slightly enriched for Oct4, and also slightly enriched for the visceral endoderm markers, Dab2 and Ihh. Both fractions expressed vascular-associated mesodermal markers (VE-cadherin, Flk1). Thus, in regular-density XENP cell cultures, SSEA1 is not suitable as a stem cell marker, and the XENP cells appear to undergo partial somatic differentiation.

Inflammatory mediators ATP and S100A12 activate the NLRP3 inflammasome to induce MUC5AC production in airway epithelial cells

Kim, Karam,Kim, Hye Jeong,Binas, Bert,Kang, Jin Hyun,Chung, Il Yup Elsevier 2018 Biochemical and biophysical research communication Vol.503 No.2

<P><B>Abstract</B></P> <P>Danger-associated molecular patterns (DAMPs) play a proinflammatory role in the pathogenesis of airway obstructive diseases such as severe asthma and chronic obstructive pulmonary disease. The NLRP3 inflammasome is a cytosolic multiprotein platform that activates the caspase-1 pathway in response to inflammatory stimuli such as DAMPs. ATP and S100 proteins are newly identified DAMPs that accumulate in inflamed airways. We previously demonstrated that S100A8, S100A9, and S100A12 induce production and secretion of MUC5AC, a major mucin in the conducting airway mucosa. The purpose of this study was to determine the involvement of NLRP3 inflammasome in, and the contribution of ATP to, S100 protein-induced MUC5AC production by NCI-H292 mucoepidermoid carcinoma cells. Stimulation with either S100A12 or ATP led to MUC5AC production at comparable levels. Simultaneous treatment with both stimuli resulted in additive increases in NLRP3, active caspase-1, IL-1β, NLRP3/caspase-1 colocalization, and MUC5AC. NLRP3 siRNA or inhibitors of NF-κB, NLRP3 inflammasome oligomerization, or caspase-1 nearly completely inhibited ATP- and S100A12-mediated MUC5AC production. Furthermore, S100A12-as well as ATP-mediated MUC5AC production was almost equally blunted by both nonspecific and specific antagonists of the purinergic receptor P2X7, a principal receptor mediating NLRP3 inflammasome activation by ATP. Thus, these two danger signals contribute to MUC5AC production in airway epithelial cells through overlapping signaling pathways for NLRP3 inflammasome activation.</P> <P><B>Highlights</B></P> <P> <UL> <LI> S100A12 activates NLPR3 inflammasomes to induce MUC5AC production in epithelial cells. </LI> <LI> ATP induces MUC5AC production in a mechanistically similar mode to S100A12. </LI> <LI> S100A12-mediated MUC5AC production involves engagement of ATP. </LI> </UL> </P>

Flower color modification through expression of Aquilegia buergeriana F3′5′H in Petunia hybrida

Lee Young Ah,천경성,Shin Ju Young,Kim Jeong Ho,Song Bina,Kim Se Jin,박필만,An Hye Ryun,Kim Yae Jin,이준대,Lee Su Young 한국원예학회 2023 Horticulture, Environment, and Biotechnology Vol.64 No.4

Aquilegia buergeriana is a native plant in Korea with blue fl owers. Flavonoid 3′,5′ hydroxylase ( F3′5′H ) is a key gene involved in the synthesis of delphinidin pigment responsible for the fl ower's blue color. We isolated the F3′5′H from the petals of A. buergeriana ( AbF3′5′H ) and introduced the AbF3′5′H gene into Petunia hybrida using Agrobacterium-mediated transformation. Forty-fi ve plants were acquired from a kanamycin-supplemented medium. Fifteen of these were identifi ed as transgenic plants using polymerase chain reaction (PCR). Quantitative real-time PCR (qRT-PCR) analysis revealed that the AbF3′5′H was expressed in the petal, corolla tube, and stigma of P. hybrida . AbF3′5′H -transgenic plant (T 0 ) fl ower color was darker than that of non-transgenic plants (NTs). Particularly, the stigma color was dramatically changed, from light yellow green (145C) to purple (N77C or N79D). The segregation ratio of the three transgenic (T 1 ) lines was identifi ed as 3:1 by PCR analysis of AbF3 ′5′H and neomycin phosphotransferase-II. The fl ower color change of the transgenic lines (T 1 ) was similar to that of T 0 . qRT-PCR analysis showed that AbF3′5′H -transgenic T 1 lines had a higher AbF3′5′H expression than NT in all fl oral organs. Moreover, delphinidin was confi rmed to be accumulated in both corolla tube and stigma and was enhanced in the petals of AbF3′5′H -transgenic T 1 lines through UPLC analysis. Our fi ndings indicate the role of AbF3′5′H in fl ower color change. These results also indicate the functionality of AbF3′5′H in bluish fl ower modifi cations

Prognostic Factors Affecting Treatment Outcomes of Acute Variceal Bleeding in Patients with Hepatocellular Carcinoma : A Single Center Prospective Study

( Yu Rim Lee ),( Soo Young Park ),( Won Young Tak ),( Young Oh Kweon ),( Se Young Jang ),( Jun Sik Yoon ),( Bina Jeong ),( Gyoun Eun Kang ),( Sang Kyung ),( Seo Keun Hur ),( Jung Gil Park ) 대한간학회 2017 춘·추계 학술대회 (KASL) Vol.2017 No.1

Aims: Because acute variceal bleeding is associated with significant morbidity and mortality in cirrhotic patients, the prediction of prognosis is important. We therefore studied about prognostic factors affecting treatment outcomes of acute variceal bleeding. In addition, factors related acute variceal bleeding in patients with HCC were also determined with particular emphasis. Methods: Endoscopy-proven acute variceal bleeding patients admitted to the Kyungpook National University Hospital between 2007 and 2013 were enrolled in this prospective cohort study. Endoscopic procedures including either endoscopic variceal ligation or sclerotherapy with N-butyl-2-cyanoacrylate were performed for the patients within 24 hours after admission. The prognostic factors were identified by applying the multivariate Cox proportional-hazards regression test using significant variables in univariate analysis. Results: 329 patients were finally included in this study. During the study period (median=15.6 months), 186 patients at a median of 4.3 months were expired. Nineteen patients were expired in 5 days, which accounted for 38.8% of 6-week mortality (n=49). Six-week mortality was related to the MELD score and 5-day treatment failure in multivariate analysis in all patients and HCC subgroup (all P<0.05). When the patients were divided into two groups by MELD score, the risk of 6 week mortality was higher in patients with MELD ≥ 15.5 than in those with MELD < 15.5 (log rank test, P<0.001). We also found that 85% of patients with both MELD score ≥ 15.5 and terminal cancer stage including mUICC stage 4 or BCLC stage D expired within six weeks and 6-week mortality risk is about 10 times higher compared to patients with lower MELD score and earlier stage after adjusting other factors (P<0.05). Conclusions: Follow-up strategy and managements are required for the individual patients, depending on these risk factors. According to our study, overly enthusiastic endoscopic treatment in patients with poor liver function and end-stage HCC may be unnecessary.

The Diagnostic Efficacy of M2BPGi for Liver Fibrosis in HCC and NAFLD Patients

( Se Young Jang ),( Won Young Tak ),( Soo Young Park ),( Young-oh Kweon ),( Yu Rim Lee ),( Bina Jeong ),( Sangkyung Seo ),( Gyoun-eun Kang ),( Gyeonghwa Kim ),( Keun Hur ),( Heon Tak Ha ),( Jae Min Ch 대한간학회 2018 춘·추계 학술대회 (KASL) Vol.2018 No.1

Aims: Mac-2 binding protein glycan isomer (M2BPGi) is recently identified as a useful non-invasive biomarker for the diagnosis of liver fibrosis. This study aimed to evaluate the diagnostic efficacy of serum M2BPGi for liver fibrosis in hepatocellular carcinoma (HCC) and non-alcoholic fatty liver disease (NAFLD) patients. Methods: M2BPGi levels were analyzed in serum samples collected from biopsy-proven HCC (n=135) and NAFLD (n=113) patients. Fibrosis was graded histopathologically in non-tumorous portion of HCC and NAFLD. Serum M2BPGi levels were determined with an automated immunoassay analyzer. Spearman’s correlation and Kruskal-Wallis test were used to evaluate the correlation and comparison among groups. Diagnostic efficacy for fibrosis was evaluated by the area under the receiver operating characteristic curve (AUC). Results: Median levels (range) of M2BPGi in HCC and NAFLD patients were 1.21 (0.12-14.33) cut-off index (COI) and 0.59 (0.13-5.90) COI, respectively. In HCC patients, fibrosis stages were 0 (n=22), 1 (n=10), 2 (n=11), 3 (n=16), and 4 (n=76). The M2BPGi levels showed a significant positive correlation (r= 0.436, P<0.001) with fibrosis grade in HCC patients and yielded the lower AUC value, 0.787 (P< 0.001) than transient elastography (TE), AUC value, 0.806 (P=0.030) to predict advanced fibrosis (F >2). In NAFLD patients, fibrosis stages were 0 (n=22), 1 (n=34), 2 (n=28), 3 (n=19), and 4 (n=10). The M2BPGi levels showed a significant positive correlation (r=0.578, P<0.001) with fibrosis grade in NAFLD patients and yielded the higher AUC value, 0.824(P< 0.001) than TE, AUC value, 0.637(P=0.035) to predict advanced fibrosis (F >2). Conclusions: Serum M2BPGi can be a useful non-invasive biomarker for predicting fibrosis in HCC and especially in NAFLD patients.