Warfarin과 Baclofen 간의 약물 상호 작용의 검증

고현윤,박재홍,신용범 大韓再活醫學會 1999 Annals of Rehabilitation Medicine Vol.23 No.6

추간판 변성의 영상화: 자기공명영상과추간판 조영술의 비교

고현윤,김훈,이종언,신용범,김학진 大韓再活醫學會 2000 Annals of Rehabilitation Medicine Vol.24 No.1

한국인에서 Quadruplex PCR을 이용한 8종류 상염색체 STR 유전좌의 다형성 분석

장영길,김기범,신창호,이숭덕,이윤성,이정빈 大韓法醫學會 2001 대한법의학회지 Vol.25 No.1

Two quadruplex PCR reactions were designed for 7 tetrameric (D3S2406, D4S2368, D5S818, D7S821, D9S925, D13S317, D19S253) and one trimeric (D6S1043) short tandem repeats loci to study the allele frequency and the applicability of genetic variation in these loci in forensic case works. For 310 unrelated Koreans DNA was isolated from peripheral blood using phenol/chloroform method. Quadruplex I was consisted of D4S2368, D6S1043, D7S821, D9S925 and quadruplex II D3S2406, D5S818, D13S317, D19S253. The amplified products were analyzed by 5% polyacrylamide gel electrophoresis followed by silver staining. The heterozygosity in each loci ranged 92.91-66.13%, and PD(Power of Discrimination) was above 085 in each loci. Every loci except D6S1043 followed hardy-Weinberg expectation. The cumulative PI was low as 1.65×10-10. Two mutations were noted, one in D19S253 and the other in D9S925 among 234 gametes. With these results above eight STR loci studied here preyed to be highly polymorphic enough to be used in forensic field. This study provides valuable population data in these loci for Korean.

제2형 당뇨병 환자에서 QUICKI법을 이용하여 측정한 인슐린 저항성과 비만도와의 관계

성윤경,최웅환,이창범,박용수 대한비만학회 2003 The Korean journal of obesity Vol.12 No.3

연구배경: 인슐린 저항성은 제2형 당뇨병의 중요한 병인으로서 비만과 밀접한 관계가 있으며, 이와 연관된 고지혈증. 고혈압의 발생은 결과적으로 혈관 합병증의 빈도를 높이는 것으로 알려져 있다. 본 연구에서는 한국인 제2형 당뇨병 환자를 대상으로 하여 새로운 인슐린 저항성 평가법인 QUICKI법을 이용하여 인슐린 저항성을 측정하고, 이와 비만도 및 체지방 분포, 지질 등의 대사성 인자들과의 상관 관계를 평가하고자 하였다. 방법: 연구 대상은 2000년 5월부터 2002년 10월까지 한양대학병원 내분비대사내과에서 당뇨병으로 치료 받은 환자 중 인슐린과 경구혈당 강하차를 투여하지 않은 103명을 차상으로 하였으며, 인슐린 저항성은 공복시 측정한 인슬린치와 포도당치를 이용하여 계산하는 QUICKI법을 이용하였다. 비만도는 입원시 키와 몸무게를 통하여 체질량지수를 측정하였으며, 체지방의 정량화와 분포를 알아보기 위하여QDR4500의 DEXA system (Hlologic, USA)을 이용하여 지방량과 건체중. 총 체지방률, 복부 지방률 등을 측정하였다. 측정 결과는 평균, 표준편차, 백분율 등으로 제시하였고 남녀간평균값의 비교 분석은 independent t-test를 시행하였다. 인슐린 저항성과 각 변수들과의 상관 관차는Pearson 상관 차수를 통해 비교하였고, 단계별 다중회귀분석을 통하여 인슐린 저항성에 가장 영향을 미치는 인자를 평가하였다. 이상의 모든 통계 분석은 SPSS (version 11.0)를 사용하였으며, 통계학적 유의 수준은 p값을 0.05 이하로 하였다. 결과: (1) 평균 연령은 55.7±12.8세, HbA_(IC)는 9.9±2.7%로 측정되었고, 체질량지수는 23.8±3.7kg/㎡', QUICKI값은 0.38±0.07로 계산되었다. (2) 남녀간에 체질량지수. EHA_(IC), 연령 등에서는 유의한 차이가 없었던 반면 QUICKI값. 총 체지방량 및 복부 지방량. 총 체지방률과 복부 지방률, 그리고 각각의 지질치에 있어서 유의한 차이가 있었다. (3) QUICKl법으로 측정된 인슐린 저항성은 HDL 콜레스테롤과 양호한 양의 상관 관계(r=0.456, p<0.01)를 보인 반면, 체질량지수(r=0.407, p<0.001), 총 체지방량(r=0.458, p<0.001), 복부 지방량((r=0.475, p<0.001), 총 체지방률(r=0.438, p<.0.01), 복부 지방률(r=0.457, p<0.01) 그리고 건체중과 대비한 총 체지방량(r=0.392, p<0.01)과 복부 지방량(r=0.423, p<0.01)과도 양호한 음의 상관 관계를 보였으며, 총 콜레스테롤(r=0.281, p<0.01), TG (r=0.333, p<0.01), LBL 콜레스테롤(r=0.326, p<0.01)과도 음의 상관 관계를 보였다. 그러나 수축기 및 이완기 혈압, HbA_(IC)와는 유의한 상판 관차를 보이지 않았다. 한편, 이러한 대사성 인자들과 QUICKI값과의 상관 관계의 정도는 HOMA_(IR)값과의 상관 관계에 비해 약간 높은 것으로 나타났다 (4) 단계별 다중회귀분석을 통하여 인슐린 저항성에 가장 영향을 미치는 인자를 분석한 결과 복부 지방률(R²=0.209, p<0.001), HDL 콜레스테롤(R²=0.135, p<0.001), 총 콜레스테롤 (R²=0.056, p<0.01) 등의 기여율이 가장 높은 것으로 나타났다. 결론: 한국인 제2형 당뇨병 환자의 인슐린 저항성을 QUICKI법을 이용하여 측정하였으며, 단순한 체질량지수나 전체 및 복부의 절대적인 지방량 보다는 복부를 구성하는 체성분중 지방이 차지하는 비율인 복부 지방률과 혈중HDL 콜레스테롤 및 총 콜레스테롤 수치가 인슐린 저항성을 가장 잘 설명하는 것으로 나타났다 따라서 이러한 인자들에 대한 개선 노력은 인슐린 저항성의 개선 및 제2형 당뇨병 발현의 억제에 기여할 것으로 판단된다. Background : Insulin resistance plays an important role in the pathophysiology of diabetes and is associated with obesity and cardiovascular disease. The aim of this study was to investigate the relationships between insulin resistance generated by quantitative insulin sensitivity check index(QUICKI) and regional adiposity, body mass index (BMI), and serum lipid profile in Korean type 2 diabetic patients. Method: Study subjects included fat mass and lean body mass by dual energy x-ray absorptiometry(DEXA) system. Insulin resistance was estimated by the QUICKI. Result :50 were women and 53 were men, with a mean (±SD) age of 54.2 years(±12.8). The mean(±SD) score of QUICKI was 0.38(±0.07). Insulin resistance was positively correlated with total body fat percent, abdominal fat percent, BMI, total cholesterol, LDL-cholesterol, and triglyceride, and negatively correlated with the HDL-cholesterol. In multivariate model, the predicting variables on insulin resistance were abdominal fat percent, HDL cholesterol, and total cholesterol. Conclusion: In this study, we observed that the abdominal fat percent, HDL cholesterol and total cholesterol were meaningful variables correlated with insulin resistance among variables. Therefore, to improve insulin sensitivity in Korean type 2 diabetic patients, we should reduce the abdominal fat percent and total cholesterol and increase the level of HDL-cholesterol.

척추손상 쥐의 지방유래 줄기세포와 미노사이클린의 운동기능 회복효과

장재혁,고현윤,차영선,박형욱,신용범,손현주 부산대학교 병원 암연구소 2009 부산대병원학술지 Vol.- No.25

내시경적 점막절제술로 치험한 식도 과립상 세포종 1례

강혁주,김성욱,최석진,이중현,장재식,서영범,윤병구,박건욱,김성자,김용섭,강승완,이구,양창헌,이창우,김욱년,이광헌,서정일 동국대학교 의학연구소 2000 東國醫學 Vol.7 No.-

과립상 세포종은 Schwann 세포 기원으로 생각되며 인체에 비교적 드물게 발생한다. 과립상 세포종은 전신 어느 곳에서나 발견될 수 있으나 주로 혀, 구강, 피부 혹은 유방 등에서 호발하며 드물게 위장관에서 발견된다. 위장관에서는 식도에서 가장 호발하며 다음으로 위, 대장 순이다. 과립상 세포종은 대부분, 특히 위장관에서는 양성이며 소수의 악성 병변이 보고되었다. 이러한 이유와 함께 수술 전의 진단이 어렵기 때문에 과립상 세포종에 대한 근본적인 치료는 현재까지 외과적 절제술이다. 최근에 시도되는 치료방법들로는 내시경적 레이저 치료, 용종절제술, 내시경적 점막 절제술 등이 있다. 저자들은 상부 소화관 내시경검사를 시행하여 식도 과립상 세포종을 진단하고 내시경적 점막 절제술을 시행하여 합병증 없이 퇴원하여 현재 재발없이 경과 관찰중인 1례를 경험하였기에 보고하는 바이다. Granular cell tumors, which occur infrequently, are probably of Schwann cell origin. They can occur almost anywhere in the body but usually affect the tongue, oral cavity, skin, or breasts and are rarely found in the gastrointestinal tracts. The esophagus is the most frequent gastrointestinal site, followed by the stomach and the colon. Granular cell tumors are generally benign, especially in the gastrointestinal tract, some malignant lesions have been reported. For this reason, and also because preoperative diagnosis is difficult, the standard treatment for granular cell tumor has until now been surgical excision. In recent years, other therapeutic methods is endoscopic laser therapy (ELT), polypectiomy, endoscopic mucosal resection (EMR). We report a case of esophageal granular cell tumor which was diagnosed by an endoscopy and managed using an endoscopic mucosal resection without complication.

Original article : Simultaneous analysis and monitoring of thirteen preservatives in cosmetics by liquid chromatography

( Yong Jae Ryu ),( Gun Mook Yoon ),( Bang Han Yun ),( Bo Young Lee ),( Pil Ki Min ),( Ji Young Yun ),( Sang Yoon Nam ),( Beom Jun Lee ),( Young Won Yoon ) 한국예방수의학회 2013 예방수의학회지 Vol.37 No.2

The content of 13 preservatives was investigated for 107 sold in retail markets. A simultaneously analytical method of liquid chromatography, LC, for the preservatives in cosmetics was developed using an AvQuityTM Ultra performance LC System, Waters, BEH C18 column, 150mmx2.1mm,1.7 ㎛, and photo Diode Array, PDA, detector λ;220 nm for quantitative analysis, λ;190∼400nm for qualitative analysis. The developed instrumental method was validated using limit of detection, LOD, limit of quantification, LOQ, precision, accuracy, and linearity of standard curves. LOD and LOQ wre lower than 0.23mg/l and 0.75mg/l, respectively. When standard compounds were analyzed seven times consecutively at the LOQ level, precision and accuracy were lower than 2.49% and, 2.04%, respectively. Good linear relationships, R2 ⊇0.9998, were obtained for all compounds. Recoveries ranged between 96.03% and 102.61%and the overall variability was below 3%, RSD, The type of cosmetics showing the lowest detection rate of target compounds was sunscreen lotion, with a detection rate of 42.9%, followed by baby lotions and mask packs with the same detection rate at 69.2% At least one of the target preservatives was detected in 82.4% of creams, 93.3% of skin toners, 95.2% of lotions, and 92.9% of body lotions. Overall 79.4% of all samples contained at least one of the target compounds. The most frequently detected compound was phenoxyethanol. Methyl and propyl paraben also had a higher detection rate than the others, except, phenoxyethanol. Although the target preservatives in cosmetics sold in retail markets were detected by the simultaneous analysis of LC, the content of the detected preservatives was within the maximum allowed amount established by the Korean FDA.

Expression Analysis of the Pistil Genes in Controlling Self-Incompatibility of Brassica campestris by CO2 Gas Using Microarray

Yong-Yoon Chung,Minjung Kwun,Youngju Choi,Hyejin Yoon,Beom-Seok Park,Bong-Joong Kang 한국분자세포생물학회 2004 Molecules and cells Vol.18 No.1

In Brassica campestris, self-incompatibility (SI) can be overcome by CO2 gas treatment. Previously, we reported inhibition of the SI in the Brassica campestris line, 734, by CO2 gas. In this study, we used a cDNA microarray comprising 1,184 unique cDNA ESTs from a pistil-specific cDNA library of the Brassica line to examine the expression of the pistil genes in response to CO2. Microarray analysis revealed that expression of 2% of the pistil genes was altered by exposure to CO2 gas. Surprisingly, the SLG gene of the susceptible line was one of the down-regulated genes. Transmission electron microscopic analysis showed that the CO2 caused morphological changes in the papillary cell. Our results demonstrate that the overcoming of SI by CO2 gas involves reduced expression of the SLG gene in Brassica.

Functional genomics study on Chinese cabbage using microarrays

Yoon Kang Hur,Myung Ho Lim,Chae Oh Lim,Yong Yoon Chung,Yong Pyo Lim,Beom Seok Park 한국육종학회 2007 한국육종학회 학술발표회 발표요지 Vol.38 No.1

Biosynthesis of Nonimmunosuppressive FK506 Analogues with Antifungal Activity

Beom, Ji Yoon,Jung, Jin A,Lee, Kyung-Tae,Hwangbo, Areum,Song, Myoung Chong,Lee, Yeonseon,Lee, Soo Jung,Oh, Ji Hoon,Ha, Sang-Jun,Nam, Sang-Jip,Cheong, Eunji,Bahn, Yong-Sun,Yoon, Yeo Joon American Chemical Society and American Society of 2019 Journal of natural products Vol.82 No.8

<P>A reduction in the strong immunosuppressive activity of FK506 (<B>1</B>) is essential for developing this compound as an antifungal agent. Seven new FK506 analogues modified at both the FK506-binding protein 12- and the calcineurin-binding regions were biosynthesized. 9-DeoxoFK520 (<B>7</B>) exhibited a >900-fold reduction in the <I>in vitro</I> immunosuppressive activity but maintained significant antifungal activity, indicating that the C-9 and C-21 positions are critical for separation of immunosuppressive and antifungal activities. <B>7</B> exhibited robust synergistic antifungal activity with fluconazole. FK506 (<B>1</B>) is a 23-membered macrolide produced by several <I>Streptomyces</I> species and is used as an immunosuppressive drug to prevent the rejection of transplanted organs. FK506 has also exhibited antifungal, neuroprotective, and neuroregenerative activities. In humans, FK506 binds to FK506-binding protein (FKBP) 12, and the resulting FKBP12-FK506 complex interacts with a Ca<SUP>2+</SUP>-calmodulin-dependent phosphatase, calcineurin (CaN). Inactivation of CaN by forming the FKBP12-FK506-CaN ternary complex prevents the activation of nuclear factor of activated T cells (NF-AT), inhibiting the production of interleukin-2 and subsequent T-cell proliferation. This CaN signaling pathway also plays a critical role in the growth and pathogenesis of major fungal pathogens such as <I>Cryptococcus neoformans</I>, <I>Candida albicans</I>, and <I>Aspergillus fumigatus</I>. Therefore, the synthesis of FK506 analogues that can discriminate human FKBP12/CaN from its fungal counterparts may separate antifungal activity from the immunosuppressive activity, thereby allowing the development of a novel antifungal agent.</P> [FIG OMISSION]</BR>