Slide Session : OS-IFD-07 ; Infectious Disease : In Vitro Antiviral Activity of Ribavirin Against Severe Fever with Thrombocytopenia Syndrome Virus

( Myung Jin Lee ),( Kye Hyung Kim ),( Jong Youn Yi ),( Su Jin Choi ),( Chung Jong Kim ),( Nak Hyun Kim ),( Kyoung Ho Song ),( Pyoeng Gyun Choi ),( Ji Hwan Bang ),( Wan Beom Park ),( Eu Suk Kim ),( San 대한내과학회 2014 대한내과학회 추계학술대회 Vol.2014 No.1

In Vitro Antiviral Activity of Ribavirin Against Severe Fever with Thrombocytopenia Syndrome Virus Myung Jin LEE1, Kye-Hyung KIM1, Jongyoun YI2, SuJin CHOI1, Chung-Jong KIM1, Nak- Hyun KIM1, Kyoung-Ho SONG1, Pyoeng Gyun CHOI1, Ji-Hwan BANG1, Wan Beom PARK1, Eu Suk KIM1, Sang-Won PARK1, Hong Bin KIM1, Nam Joong KIM1, Myoung- Don OH1 Seoul National University College of Medicine, Korea1, Pusan National University School of Medicine, Korea2 Background: Severe fever with thrombocytopenia syndrome (SFTS) is an emerging infectious disease caused by a novel Bunyavirus, severe fever with thrombocytopenia syndrome virus (SFTSV). No effective antiviral therapy is proven yet, but clinical use of ribavirin (RBV) has been tried. We investigated the antiviral effect of RBV against SFTSV in vitro. Methods: To test for cytotoxicity of RBV, Vero cells were treated with different concentrations of RBV (3.90 to 500 μg/mL, two-fold dilution) and analyzed by cell viability MTS assay 48h post-infection. To determine antiviral activity of RBV against SFTSV, Vero cells were infected with SFTSV strain Gangwon/Korea/2012 at 100 TCID50 (50% tissue culture infective dose) per well in a 96-well plate, and RBV was added at the concentrations showing no or minimal cytotoxicity. Viral RNAs were extracted from the culture supernatants and quantifi ed using one-step real-time reverse transcription- PCR to amplify the partial large segment of SFTSV. Statistical analysis was done by one-way ANOVA with Tukey`s post hoc test. Results: Cytotoxicity due to RBV was not observed at RBV concentration =31.3 μg/ mL. Viral RNAs at 24h post-RBV treatment were reduced with increasing RBV concentrations (1-32 μg/mL), compared with those of mock-treated cells (P <0.01, Figure). Half maximal inhibitory concentration (IC50) of RBV was 3.69 μg/mL at 24h post-RBV treatment. Conclusions: Our study shows that RBV has antiviral effect against SFTSV in a dose-dependent manner. Further studies are required to evaluate the effi cacy of RBV in SFTS.

한국인 직무 스트레스 측정도구의 개발 및 표준화

장세진,고상백,강동묵,김성아,강명근,이철갑,정진주,조정진,손미아,채창호,김정원,김정일,김형수,노상철,박재범,우종민,김수영,김정연,하미나,박정선,이경용,김형렬,공정옥,김인아,김정수,박준호,현숙정,손동국 大韓産業醫學會 2005 대한직업환경의학회지 Vol.17 No.4

Background and Purposes: Over the past three decades, numerous studies performed in Korea have reported that job stress is a determinant risk factor for chronic diseases and work disability. Every society has its own culture and occupational climate particular to their organizations, and hence experiences different occupational stress. An occupational stress measurement tool therefore needs to be developed to estimate it objectively. The purpose of this study is to develop and standardize the Korean Occupational Stress Scale (KOSS) which is considered to be unique and specific occupational stressors in Korean employees. Subjects and Methods: Data were obtained from the National Study for Development and Standardization of Occupational Stress (NSDSOS Project: 2002-2004). A total of 12,631 employees from a nationwide sample proportional to the Korean Standard Industrial Classification and the Korean Standard Occupational Classification were administered. The KOSS was developed for 2 years (2002-2004). In the first year, we collected 255 items from the most popular job stress measurement tools such as JCQ, ERI, NIOSH and OSI, and 44 items derived from the a qualitative study (depth interview). Forty-three items of KOSS, in the second year, were retained for use in the final version of the KOSS by using Delphi and factor analysis. Items were scored using conventional 1-2-3-4 Likert scores for the response categories. Results: We developed eight subscales by using factor analysis and validation process: physical environment (3 items), job demand (8 items), insufficient job control (5 items), interpersonal conflict (4 items), job insecurity (6 items), organizational system (7 items), lack of reward (6 items), and occupational climate (4 items). Together they explained 50.0% of total variance. Internal consistency alpha scores were ranged from 0.51 to 0.82. Twenty-four items of the short form of the KOSS (KOSS-SF) were also developed to estimate job stress in the work setting. Because the levels of the subscales of occupational stress were gender dependent, gender-specific standard norms for both the 43-item full version and the 24-item short form using a quartile for the subscales of KOSS were presented. Conclusion: The results of this study suggest that KOSS might be an appropriate measurement scale to estimate occupational stress of Korean employees. Further and more detailed study needs to be conducted to improve the validity of this scale.

서울의 Penicillinase Producing Neisseria gonorrhoeae 발생빈도(1998)

김재홍,김준호,반재용,이정우,황성주,정준규,정성태,강진문,조흔정,홍창의,정혜신,이한승,김이선,이봉길,이종호,선영우,한기덕,윤성필,이성훈,안종성,박석범,문승현,조항래,김형섭,류지호,황재영,박준홍,손상욱 한양대학교 의과대학 2001 한양의대 학술지 Vol.21 No.1

In recent years, gonorrhea has been pandemic and remains one of the most common STDs in the world, especially in developing countries. For the detection of a more effective therapeutic regimen and assessing the prevalence of Penicillinase Producing Neisseria gonorrhoeae(PPNG), we have been trying to study the patients who have visited the Venereal Disease Clinic of Choong-Ku Public Health Center in Seoul since 1980 by menas of the chromogenic cephalosporin method. In 1998, 93 strians of N. genorrhoeae were isolated, among which 60(64.5%) were PPNG. The prevalence of PPNG in Seoul, which had been decreased to 39% in 1996 after a peak of 74.3% in 1993, is increased to 64.5% in 1998.

적색 석류석 보석의 보석·광물학적 특징

김금조,김진섭,김원사,최진범 한국광물학회 2003 광물과 암석 (J.Miner.Soc.Korea) Vol.16 No.1

보석으로 사용되고 있는 파이로프-알만딘 계열의 보석을 대상으로 하여 이들의 화학성분, 결정구조, 굴절율, 비중, 색, 광택 등의 광물학적 성질을 알아보았다. 또한 위 고용체의 광물학적 성질이 화학성분의 치환 정도에 따라 점이적인 변화 양상이 나타났는지에 대해서도 연구하였고, 발색소에 대한 연구를 실시하였다. 특별히 보석업계에서 관행적으로 분류 기준으로 삼아온 굴절율과 비중 값이 광물학적으로 오류가 없는 것인지를 검증하는 것 등도 주요 연구목적에 포함되었다. 적색 석류석 시료 중 화학성분은 따른 분류기준에 의하면 17개가 파이로프이며, 6개가 알만딘임을 알수 있다. 파이로프의 굴절율은 1.77까지이며, 알만딘은 그 이상임을 알 수 있으며, 비중은 파이로프인 경우 3.88까지의 범위이며, 파이로프는 적어도 4.11 이상임을 알 수 있었다. 따라서 보석업계에서 파이로프와 알만딘을 구분하여 온 굴절율 기준(R.1. 1.75)은 오류이며, 비중값 기준 (S.G. 3.88)은 무난한 것으로 밝혀졌다. 단결정 X-선회절분석 결과에 따르면, 파이로프-알만딘 석류석이 모두 등축정계의 Ia3d 공간군에 속하며, 화학성분의 변화에 따른 단위포의 크기는 변화를 느낄 정도의 차이는 없었다. 또한 굴절율과 비중은 파이로프-알만딘 고용체에 있어 특히 FeO 함량에 따라 일정한 변화양상을 나타내는데, FeO의 함량이 증가할수록 굴절율과 비중이 증가함을 알 수 있다. 화학분석 자료에 의하며, 적색 및 보라색은 Fe^(+2), 황색은 Mn^(+2) 함량에 따라 크게 좌우됨을 알 수 있다. 적색 석류석내에 들어있는 결정 내포물은 주로 저어콘과 금홍석이었다. Chemical composition, crystal structure, refractive index, specific gravity, color, and luster were studied for pyrope-almandine series garnets. The main coloring agents determining the reddish or brownish garnets were also investigated. It was also examined if there is any relationship between mineralogical properties with respect to the various chemical compositions in the solid solution, in the hope to figure out the existing classification values of R.I. and S.G. using gemtesting facilities to distinguish pyrope from almadine. It was found that 17 out of the 24 specimens belong to pyrope and the rest almandine. R.I. of pyrope goes up to 1.77 and that of almandine is higher than the value. S.G. of pyrope reaches to 3.88 and that of almandine is greater than the value of pyrope. X-ray diffraction data revealed that pyrope-almandine garnets are isometric with space group Ia3d , and also show that the variation of cell parameters are not significant enough to parallel with the chemical compositions of the series. R.I. and S.G. increase with FeO content. Fe and Mn are most responsible to the red-purple and orange coloration of the specimens, respectively. Both zircon and rutile crystals are most common inclusions in the reddish stones.

The comparison of gene expression of inflammatory mediators between palmoplantar pustulosis and pompholyx

( Do Young Kim ),( Ji Young Kim ),( Hyojung Sohn ),( Taegyun Kim1 ),( Jihun Park ),( Beom Jin Lim2 ),( Sang Ho Oh ) 대한피부과학회 2012 대한피부과학회 학술발표대회집 Vol.64 No.3

재조합 대장균의 고농도 유가식 배양에 의한 인체 Granulocyte-Colony Stimulating Factor 생산

김범수,유미진 建陽大學校 1999 建陽論叢 Vol.- No.7

Fed-batch fermentation of recombinant Escherichia coli harboring the gene encoding mature human granulocyte-colony stimulating factor (hG-CSF) fused to the gene for the pectate lyase B (pelB) signal peptide of Erwinia caratovora was performed. The fusion protein, pelB-hG-CSF, was produced at a concentration of ca. 1.7 g/L in an inclusion body form.

척추경 나사의 인출하중 시험 및 평가

김상범, 주진원 충북대학교 건설기술연구소 2018 建設技術論文集 Vol.37 No.2

본문 참조

접촉산화수로공법에 의한 수질개선연구

김태철,이규승,오범룡,민진우 忠南大學校 環境問題硏究所 1998 環境硏究 Vol.16 No.-

The effect of non-point source pollution in rural area has much influence on the stream water quality. It is difficult to improve the stream water quality, because of complex pollutant loads technically and because of treatment-cost of the deteriorated water economically. The best way to reduce the non-point source pollution is to check the water quality in the inlet of irrigation channel and control the water quality in the outlet of drainage channel. Stream water quality in the rural area is closely related with the fertilizers, pesticides, and livestock wastewater. Basically the rate of treatment and retention time were estimated for BOD, COD, SS, NH₄-N, T-N, NO₃-N, T-P, PO₄-P using materials such as gravel, crushed stone, useless tire, geotextiles, and concrete block, respectively and also using the combined materials. The rate of treatment for BOD, COD, SS is high, but that for NH₄-N, T-N, NO₃-N, T-P, PO₄-P is low. Optimal retention time in the natural contact channel was 90 minutes.

대학생의 상담에 대한 태도

김범선,전윤경,전진실 西江大學校 學生生活相談硏究所 2008 人間理解 Vol.29 No.-

본 연구는 성별, 연령 등의 인구학적 변인과 이전 상담 경험여부가 상담에 대한 태도에 어떠한 영향을 미치는 가를 알아보기 위해 실시되었다. 이를 위해 서울시내 S 대학에 재학 중인 528명의 학생들에게 상담에 대한 태도와 이전 상담 경험, 그리고 인구학적 특성들을 알아보는 설문지에 응답하게 한 후 그 결과를 통계적으로 분석하였다. 상담에 대한 태도는 Fisher 와 Turner(1975)의 상담태도에 관한 질문지로 측정되었다. 그 결과, 인구학적 변인 중 성별, 학년, 소속 학과, 재수 여부, 종교, 그리고 이전 상담 경험이 상담에 대한 태도에 영향을 미치는 것으로 나타났다. 즉, 여성이 남성에 비해, 천주교 집단이 다른 종교 집단에 비해 전문가에 대한 신뢰와 상담 욕구 면에서 유의미하게 높게 나타났고, 4학년 집단이 1학년에 비해, 문학부가 다른 학부에 비해 전문가에 대한 신뢰의 측면에서 유의미하게 높은 점수를 보였다. 재수를 하지 않은 집단이 삼수이상 집단에 비해 오점수용에서 더 유연한 것으로 나타났으며, 이전에 상담을 해 본 사람일수록 상담에 대한 태도도 더 긍정적이고, 상담전문가를 더 신뢰하며, 오점 수용에 유연하고, 상담 욕구도 높은 것을 알 수 있었다. 마지막으로 이를 통해 얻을 수 있는 논의점과 본 연구의 제한점, 후속 연구를 위한 제안점을 기술하였다. This study investigated college students' attitude toward counseling according to gender, age, and other demographic variables. Research participants were 528 S college students in Seoul. They filled out the Attitude Toward Seeking Professional Help Scale(Fisher & Turner, 1975) and demographic guestionnaire. To analyze data, correlation analysis and ANOVA were conducted. As results, gender, grade, major failure in previous college entrance exam, religion and previous counseling experience had significant influence on attitude toward counseling. Results indicated that women and catholic groups showed more positive attitude in Confidence and Need of seeking attitude toward counseling than other groups. The college seniors and the departs of literature groups showed more positive tendency in Confidence of seeking attitude toward counseling than others. Students who had not repeated a college entrance exam had more flexible attitude in Stigma. Students with previous counseling experience showed more positive tendency in Need, Stigma, Confidence and the whole attitude toward counseling. Discussions and limitations on this study were described.

캡사이신유도체를 함유하는 폴록사머 겔제제의 물리화학적 특성 : based Gel Containing Capsaicin Analog

김태완,조청일,최춘영,이범진 한국약제학회 2003 Journal of Pharmaceutical Investigation Vol.33 No.1

Physicochemical changes of poloxamer-based gel containing capsaicin analog (N-[3-(3,4-dimethylphenyl)propyl]-4-(2-aminoethoxy)-3-methoxypheny] acetamide) such as drug content, viscosity and surface tension were investigated during the storage conditions at three different temperatures (25, 40 and 60℃) over 90 days. No noticeable changes of color were observed when stored at 25 and 40℃. However, the color of white poloxamer gels turned yellow during storage at 60℃. The drug contents were unchanged during storage at 25℃ but had tendency to decrease at 40℃. The drug contents were highly decreased over 40-50% when stored at 60℃. The viscosity of a poloxamer-based gel was unchanged during storage at 25 and 40℃ but greatly increased at 60℃. The surface tension of a poloxamer-based gel was not changed at three different temperatures. The storage conditions of a poloxamer-based gel containing capsaicin analog can be considered for further clinical applications.