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Pocine Adenovirus-3의 E1B Region의 鹽基序列 分析
朴鍾賢,宋載永,李重馥,玄芳勳,安東濬,車相昊,裵用泰,姜永源,Reddy, P S,全茂炯,安壽煥 충남대학교 생물공학연구소 1999 생물공학연구지 Vol.7 No.-
돼지 아데노바이러스(PAV-3). 6618주의 EIB region이 包含되어 있는 map unit 4.0에서 9.7까지의 유전자에 대한 1,984 bp의 염기서열을 決定하였으며, 이 結果를 알려진 여러 아데노바이러스 유전자와 비교하여 다음과 같은 결과를 얻었다. 1. PAV-3의 EIB유전자는 10개의 ORF로 구성되어 있으며, 그 중 아데노바이러스의 단백질과 유사성이 있는 것은 ORF1, ORF2 및 ORF3이었다. ORF1은 Ad41의 19kd 와 BAV-2에서의 EIB ORF2에서의 아미노산의 一致率은 각각 32%와 31%이었다. 2. ORF2는 Ad2 55kd protein과 tupaia adenovirus 44kd protein가 각각 34%로 아미노산 一致率이 가장 높았으며, Ad41의 52kd protein. BAV-3의 EIB ORF3에서도 33%의 一致率을 보였다. 3. ORF1은 61-666 uncleotide (606 bp), ORF 2에서는 429-1,850 uncleotide (1,422 bp)의 부위로 각각 202, 474 a.a로 構成되었으며, 예상되는 분자량은 20 kd와 52 kd이었다. 4. ORF3는 hexon-associated pIX유전자로 추정되며 내부에 1개의 polyadenylation signal(ATAAA)이 1938-1942 uncleotide에 위치하였으며, 이 부위는 TATA box (1937-1942 uncleotide)와 중복되어 존재하였다. Porcine adenovirus type 3 (PAV-3) does not cause severe infection in pigs. Adenovirus has been suggestive of live vaccine vector carrying foreign gene. One of insertion regions is delayed early (EIB) region. However, EIB region of PAV-3 has not been molecularly characterized to date. Nucleotide sequence of EIB of PAV-3 was determined. The EIB region was composed of 1,984 bp and located between 4.0 and 9.7 map units. Three potential open reading frames(ORFs) with low level of homology to other adenoviruses and a polyadenylation signal were identified in the rightward direction of genome. The nucleotide and the predicted amino acid sequences of EIB were compared to those of human and animal adenoviruses. One of the three potential ORFs. ORF1 encoded a polypeptide homologous to bovine adenovirus type 2(BAV-2) ORF2 and human adenovirus type 41(Ad41) 19 kd protein. ORF2 encoded a polypeptide homologous to human adenovirus type 2(Ad2) 55 kd protein, bovine adenovirus type 3(BAV-3) ORF3 and porcine adenovirus type 4(PAV-4) ORF2. The predicted protein of ORF1 had homology to those of Ad41 and BAV-2 with 32 and 31% respectively, whereas the deduced protein of ORF2 had homology to those of Ad2. BAV-3 and PAV-4 with 34, 33 and 29%, respectively.
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Baculovirus를 利用한 Canine Parvovirus VP2蛋自質의 發現
朴鍾賢,宋載永,玄芳動,安動濬,姜永源,全茂炯,安壽煥 충남대학교 생물공학연구소 1998 생물공학연구지 Vol.6 No.-
국내에서 分離된 개파보바이러스주(V20주)의 VP2 遺傳子를 baculovirus system을 이용하여 발현시켜 다음의 結果를 얻었다. 1. 개파보바이러스의 VP2 유전자를 PCR에 의해 增幅하여 1755bp의 VP2遺傳子를 pUC19에 클로닝하여, 클로닝된 遺傳子를 polyhedrin promoter를 가지는 baculovirus expression vector인 pVL1393에 옮겨 VP2 발현벡터인 pVL1393-VP2를 얻을 수 있었다. 2. pVL1393-VP2 plasmid와 baculovirus DNA와의 homologous recombination에 의해 재조합바이러스인 VP2-BV를 얻을 수 있었으며, 그 발현효율은 2.000-5.000 HAU/0.05 ml이었다. 3. 免疫沈澱法에 의해 발현된 단백질은 개파보바이러스의 VP2단백질과 유사한 64 kb에 달하는 것이었으며, 血球凝集能을 지니고 있었다 4. 血球凝集能을 지닌 蛋白質이 여러 陽性血淸에 있어서 抗體수준을 測定할 수 있는지 개파보바이러스항원과의 相關性을 比較한 바 0.94 (n=125. p<0.01)의 相關係數를 보였다 5. 발현 VP2白은 virus-like particles를 形成하였으며, 그 크기로는 개파보바이러스와 비슷한 25 ㎚의 크기를 갖았다. Canine parvovirus(CPV) is a member of autonomous replicating parvoviruses and is aetiologically associated with enteritis and mycoarditis in puppies. The capsids of CPV are composed of three structural proteins: VP1, VP2 and VP3. The VP2 protein is the major component of capsid. The VP2 gene of a canine parvovirus. V20 strain isolated in Korea was cloned into baculovirus expression vector, and subsequently the VP2 protein was expressed by a recombinant baculovirus under the control of polyhedrin promoter. The recombinant VP2 protein expressed in Spodoptera frugiperda 9(Sf9) cells was detected by haemagglutination(HA) test and immunofluorescent antibody assay. Molecular weight of the recombinant VP2 protein expressed was estimated as 64Kd when tested by immunoprecipitation test using anti-CPV monoclonal antibody. In haemagglutination inhibition(Ⅲ) test. 8 HA units of the recombinant VP2 protein antigen was successfully utilized to determine a level of antibody against CPV in various positive sera. The recombinant VP2 protein showed also the capability to form virus like particles similar in size and appearance to the CPV virions.
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Kang, Bang-Hun,Lee, Joon-Ho,Park, Jong-Kyun The Ecological Society of Korea 2012 Journal of Ecology and Environment Vol.35 No.1
In the current study, several carabid beetle species were proposed as potential biological indicators for different habitat types (levee, upland dike, hillock, and streamside) of agricultural landscapes focusing on agrobiont species. Synuchus arcuaticollis and Synuchus orbicollis were found in all habitat types, indicating that they are general species for all habitat types. Harpalus eous and Synuchus cycloderus are potential bioindicator species for the paddy levee and hillock habitats, respectively. Amara pseudosimplicidens, Anisodactylus punctatipennis, and Chlaenius ocreatus, which occurred widely, and Bembidion morawitzi, which occurred only in the streamside habitat, are potential bioindicators for the streamside habitat.
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점봉산 천연보호림 지역의 날개응애 (Acari :Oribatida) 목록
Bang Hun KANG 한국응용곤충학회 1996 Insecta Koreana Vol.13 No.-
점봉산 천연보호지역에서 식생이 다른 4지역을 대상으로 한 날개응애류 생물상 조사에서 총 52과 81속 143종이 조사되었다. 고도 900m의 잣나무 조림지에서는 33과 48속 102종, 고도 1,100m의 혼효림지역에서는 38과 57속 97종, 고도 1,000m의 천연 활엽수림지역에서는 하부 식생이 일년생 초본류인 복사면에서는 36과 49속 72종, 하부식생이 조릿대인 남자면에서는 44과 63종 102종이 조사되었다. A total of 143 species belonging to 81 genera under 52 families of oribatid mites were collected at four sites in Mt. Jumbong which is a nature reserve area in Korea (102 spp., 48 gen, 33 fam. at the plantation of Korean pine in alt. 900m; 97 spp., 57 gen., 38 fam. at the mixed forest in alt. 1, 100 m; 72 spp., 49gen., 36 fam. at the north-facing slope site where lower vegetation was annual herbaceous; 102 spp., 63 gen., 44 fam. at the south-facing slope site where lower begetation was perennial sasa in alt. 1, 000 m; and there slpes were adjacent to each other and composed of deciduous trees).
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점봉산 천연보호림 지역의 날개응애 (Acari :Oribatida) 목록
Bang Hun KANG 한국응용곤충학회 1996 INSECTA KOREANA Vol.13 No.-
점봉산 천연보호지역에서 식생이 다른 4지역을 대상으로 한 날개응애류 생물상 조사에서 총 52과 81속 143종이 조사되었다. 고도 900m의 잣나무 조림지에서는 33과 48속 102종, 고도 1,100m의 혼효림지역에서는 38과 57속 97종, 고도 1,000m의 천연 활엽수림지역에서는 하부 식생이 일년생 초본류인 복사면에서는 36과 49속 72종, 하부식생이 조릿대인 남자면에서는 44과 63종 102종이 조사되었다. A total of 143 species belonging to 81 genera under 52 families of oribatid mites were collected at four sites in Mt. Jumbong which is a nature reserve area in Korea (102 spp., 48 gen, 33 fam. at the plantation of Korean pine in alt. 900m; 97 spp., 57 gen., 38 fam. at the mixed forest in alt. 1, 100 m; 72 spp., 49gen., 36 fam. at the north-facing slope site where lower vegetation was annual herbaceous; 102 spp., 63 gen., 44 fam. at the south-facing slope site where lower begetation was perennial sasa in alt. 1, 000 m; and there slpes were adjacent to each other and composed of deciduous trees).
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강방훈 ( Bang Hun Kang ),조승진 ( Seung Jin Cho ),손진관 ( Jin Kwan Son ),신지훈 ( Ji Hoon Shin ) 한국농촌계획학회 2012 농촌계획 Vol.18 No.3
This study aimed to investigate the images that affect general travel behavior and satisfaction of eco-tourists of Section seven of Jeju Olle-gil and to give eco-tourists preferred images to newly constructed eco-tour sections. Adjectives were selected to evaluate images, and for analysis, a survey was carried out with 132 persons who have toured 7 section of Jeju Olle-gil. On this survey, basic travel type, overall satisfaction, image before and after experience, age, gender, satisfaction level and accompanying type were inquired. 81(61.4%) knew section 7 of Jeju Olle-gil ``in advance``, and 58(39.1%), which takes up the largest portion in this question, came to choose section 7 by a word of mouth. 95(71.9%) answered that they stayed 2 nights and 3 days, and 55(41.7%) answered that they stayed at a pension as accommodation. As for accompanying type, 34(25.7%) answered they accompanied family and relatives. As for the decision of visit, 60(45.5%) replied that they decided one month prior to the visit. And as for the purpose of visit, 63 (33.8%) replied they visited to appreciate nature. The adjectives that demonstrate overall satisfaction and significance level of the tourists were ``placid``, ``refreshing``, ``living``, ``mountainous`` and ``green``. As for the satisfaction level, people were satisfied with its environment, cleanliness level and direction boards. The types of section preferred by the tourists were clay pavements, followed by sand-masa soil mixture pavement and wooden deck pavement. ``Oidolgae`` section was selected as the representative section, and 10:00a.m, ~12:00p.m, was preferred as visiting time. And it is also proved that people were positive in appointing photo zones.
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