Effect of Salmonella pullorum on Immunological response in Poultry

Baatartsogt,Hee-Kyung Lim,Jeong-Soo Lee,Sung-Kwon Kim,Ji-Hyuk Kim,Kang-Duk Choi 한국가금학회 2006 한국가금학회 정기총회 및 학술발표회 Vol.23 No.-

Salmonella pullorum is the cause of pullorum disease, which is characterized by white diarrhea and a high mortality rate in poultry. Ross infected with S. pullorum at 2 weeked. Total RNA was extracted from Ross spleen lymphocytes after tRNA clean up to used RNeasy MinElute Cleanup kit(Qiagen). We are investigated the expression of Haem oxygenase, N-cadherin, C-type lectin short form; c-est-1: c-ets-1 genes was increased in fowl typhoid by RT-PCR analysis. The expression of tumor necrosis factor receptor-II, Glutathione S-transferase and beta-defensin gene was decreased in fowl typhoid.

Detection and analysis of immunological genes in response to the Infection of Salmonella gallinarum in chicken

Baatartsogt. O,H. K. Lim,Urantulkhuur. B,S. W. Suh,J. D. Kang,B. G. Jang,J. H. Kim,S. H. Kim,J. H. Lee,K. D. Choi 한국가금학회 2007 한국가금학회 정기총회 및 학술발표회 Vol.24 No.-

Fowl typhoi d (FT) i s a severe systemic disease of chickens causing heavy economic losses to the poultry industry through mortality, reduced egg production and culling of precious breeding stocks. It is hard to be discovered by clinical sign, pathology and immunology. The immune response to S. gallinarum is poorly characterized because of its virulence. In this study, the total RNA isolated by spleen lymphocytes for Affymetrix GeneChip and RT-PCR analysis. Total RNA was extracted using TRIzol (Invitrogen, Carlsbad, CA). These results indicate that expression of LY86, FN1, K60, CCL19, ah22 increased and during immune response at chickens B-FVI was decreased in fowl typhoid. In conclusion, the differential expressed genes in spleen lymphocytes from S. gallinarum infected and uninfected control groups were related with immunological defense system of chickens. The several identified gene may be used as valuable in formation for immune response mechanims of FT biology.

Immuno-regulatory Effect of Dryopteriscrassirhizoma Extract on Chicken Splenocyte

Baatartsogt. O,Urantulkhuur. B,S.W. Suh,H.K. Lim,Y.K. Kim,K.O.Park,J.H. Kim,S.H. Kim,P. K. mandal,K. D. Choi 한국가금학회 2008 한국가금학회 정기총회 및 학술발표회 Vol.25 No.-

We investigated the effect of herbal extract of Dryopteris crassirhizoma on the cell viability, immune response, and nitric oxide production of chicken splenocyte. Nitrite levels were assayed in the culture supernatant of splenocyte after 24h by the Griess method. The expressions of pro-inflammatory cytokines such as interleukin (IL-2, IL-6, IL-8, IL-18) and the housekeeping gene GAPDH were studied using RT-PCR. Splenocyte were cultured with the herbal extracts at 37℃ in 96-well plate for 24h. Not cytotoxic effect was observed at 20 ㎍ and 40 ㎍. The NO production increased dose dependently with the treatment of herbal extract. which suggest that this herbal extract has no anti-inflammatory effect. In cytokine expression it was observed that the herbal extract induced the expression of IL-2 and suppressed the expression of IL-6 and IL-18, however there was no effect on the expression of IL-8. It may be concluded that the Dryopteris crassirhizoma extract is non toxic and have no anti-inflammatory effect and it has immune stimulation effect through IL-2.

Polymorphism of Natural Resistance-Associated Macrophage Protein 1 Gene in Hy-Line Chicks infected Salmonella gallinarum

Baatartsogt. O,Erdenesuvd. J,Suh S.W,Kim Y.K,Kim Y.Y,Anh K.C,Lim H.K,Choi Kangduk 한국가금학회 2009 한국가금학회 정기총회 및 학술발표회 Vol.26 No.-

Salmonella gallinarum (S. gallinarum) is the causative agent of fowl typhoid, a severe systemic disease of chickens that results in high mortality amongst infected flocks. Due to its virulence, the immune response to S.gallinarum is poorly characterized. The natural resistance-associated macrophage protein 1 (NRAMP1) affects host innate immunity to intracellular bacteria because of its ability to transport divalent cations in late endosome/lysosomes. The objectives of this study were to identify single nucleotide polymorphisms of the NRAMP1 gene in Hy-Line chicks by a polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). A PCR-RFLP method was developed to identify a SacI polymorphism for Ser³??. To investigate associations between an NRAMP1 SNP and resistance against S.gallinarum, two NRAMP1 alleles of a T and C were analyzed to 3 genotypes, TT (802 bp), TC (802, 723 bp and 79 bp), and CC (723 and 79 bp). We evaluated the effect of 3 genotypes, such as TT(66 %), CC (36.9 %) and TC (41.9 %) alleles on mortality after S. gallinarum challenge.

Proteomic Analysis of Differential Protein Expression in Fibroblast-like Synoviocytes of Pig

O. Baatartsogt.,So. Hyun-Kyung,Cho. In-Hee,Lee. Jeong-Soo,Lim. Hee-Kyung,Lee. Jong-Ha,Kim. Eun-Kuk,Choi. Kung-Duk 한국축산식품학회 2006 심포지움 및 학술발표회 Vol.- No.37

The innermost structures of synovium consist of one to three layers of cells generally identified as synovial lining cells(SLC). The present studies were initiated to determine the protein expression patterns of fibroblast-like synovial(FLS) cells derived from the synovia of rheumatoid arthritis. Post-traumatic arthritis(PTA) is one of the most common causes of secondary osteoarthritis, and usually affects younger people. The proteins were separated by two-dimensional polyacrylamide gel electrophoresis and RNA expression investigated by RT-PCR Proteome analyses led to the identification of more than 1,500 protein spots and of 11 differently expressed protein spots among them. Six proteins were down-regulated, and five proteins were up-regulated in ACL-transected synovial tissue. Among these, spots 3 and 8 were identified as cofilin-1 and smooth muscle protein 22-α, respectively, Therefore, the proteome analysis of synovial tissue is a useful approach to investigate a joint after an injury and can be used to understand the pathogenesis of PTA.

효모추출물의 사료 첨가가 성장과 사이토카인의 발현 변화에 미치는 영향

임희경,Baatartsogt. O,서상원,김희철,이종석,안광철,김상호,김지혁,장병귀,이준헌,이상범,최강덕 한국가금학회 2007 한국가금학회 정기총회 및 학술발표회 Vol.24 No.-

The experiment was conducted to compare the effects of supplements of yeast extract on growth and immune responses of chicks fed diets. Total 120 of chicks were employed in this experiment to study the effects of feeding of yeast extract on the performance and expression of cytokine. They were randomly allocated to tree dietary treatments; control, antibiotic group(ABG), yeast extract group(YEG). Growth characteristics by feeding yeast extract were significantly difference on body weight. Feed intake contained yeast extract was 69g daily and showed higher than other group. To evaluate the immune response effects of the feeding yeast extract, the expression patterns of IL-2, IL-6, IFN-γ in spleen cell were studied by RT-PCR. The higher expression levels of IL-2, IL-6 and IFN-γ transcripts after Salmonella gallinarum infection were observed in the group with yeast extract. From the result of this experiment, it could be concluded that feeding yeast extract improves the laying performance growth and immune response activation.

가금에 있어서 Salmonella gallinarum 감염에 대한 분자생물학적 반응

임희경,Baatartsogt Oyungere,이정수,장병귀,김지혁,김동욱,최강덕 한국가금학회 2006 한국가금학회 정기총회 및 학술발표회 Vol.23 No.-

Fowl typhoid is a septicemic disease of domestic birds caused by Salmonella gallinarum. The out break of Fowl typhoid (FT) is characterized by increased mortality, anorecia, greenish-yellow diarrhea, and a drop on egg production. The purpose of this study was to investigate the different expressed chicken spleen lymphocyte control and disease fowl typhoid using selected immune response gene. And this result may be used as valuable information for fundamental mechanisms of fowl typhoid biology and biomarkers.

고온 스트레스 시 녹차 산물의 급여가 닭의 면역 세포 구성에 미치는 영향

임희경,Baatartsogt. O,소현경,P.K. Mandal,한다운,김동욱,강환구,김상호,김지혁,이준헌,최강덕 한국가금학회 2008 한국가금학회 정기총회 및 학술발표회 Vol.25 No.-

This study was conducted to evaluate the effect of green tea supplement on the CD4 and CD8 T-cells in broiler during heat stress. One hundred twenty 5 weeks old broiler chicken were divided into 4 groups. One group was used as control and other groups were given green tea supplement through feed at the rate of 0.5%, 1.0%, 3.0% for 10 days and then kept under heat stress for 3 days at 35 ℃. CD4 and CD8 T-cells were counted by flow cytometry at 0, 1, and 3 day. It was observed that the level of CD4 T-cell percent has increased from 0 day to 3 day in all the treatment groups. CD8 T-cells have gradually decreased in control. however. in treatment groups it has first decreased in 1 day and then incresed at 3 day. It may be concluded that feed supplement with green tea at the level of 3% is useful to boost immunity during heat stress.

Genetic diversity and population structure of Mongolian regional horses with 14 microsatellite markers

윤지혜,Oyungerel Baatartsogt,공홍식 아세아·태평양축산학회 2022 Animal Bioscience Vol.35 No.8

Objective: This study aimed to identify the genetic diversity and population structure of Mongolian horse populations according to the province of residence (Khentii, KTP; Uvs, USP; Omnogovi and Dundgovi, GOP; Khovsgol, KGP) using 14 microsatellite (MS) markers. Methods: A total of 269 whole blood samples were obtained from the four populations (KTP, USP, GOP, KGP) geographically distinct provinces. Multiplex polymerase chain reaction (PCR) was conducted using 14 MS markers (AHT4, ASB2, ASB17, ASB23, CA425, HMS1, HMS2, HMS3, HMS6, HMS7, HTG4, HTG6, HTG7, and VHL20), as recommended by the International Society for Animal Genetics. Capillary electrophoresis was conducted using the amplified PCR products, alleles were determined. Alleles were used for statistical analysis of genetic variability, Nei’s DA genetic distance, principal coordinate analysis (PCoA), factorial corresponding analysis (FCA), and population structure. Results: On average, the number of alleles, expected heterozygosity (HExp), observed heterozygosity (HObs), and polymorphic information content among all populations were 11.43, 0.772, 0.757, and 0.737, respectively. In the PCoA and FCA, GOP, and KGP were genetically distinct from other populations, and the KTP and USP showed a close relationship. The two clusters identified using Nei’s DA genetic distance analysis and population structure highlighted the presence of structurally clear genetic separation. Conclusion: Overall, the results of this study suggest that genetic diversity between KTP and USP was low, and that between GOP and KGP was high. It is thought that these results will help in the effective preservation and improvement of Mongolian horses through genetic diversity analysis and phylogenetic relationships. Objective: This study aimed to identify the genetic diversity and population structure of Mongolian horse populations according to the province of residence (Khentii, KTP; Uvs, USP; Omnogovi and Dundgovi, GOP; Khovsgol, KGP) using 14 microsatellite (MS) markers.Methods: A total of 269 whole blood samples were obtained from the four populations (KTP, USP, GOP, KGP) geographically distinct provinces. Multiplex polymerase chain reaction (PCR) was conducted using 14 MS markers (AHT4, ASB2, ASB17, ASB23, CA425, HMS1, HMS2, HMS3, HMS6, HMS7, HTG4, HTG6, HTG7, and VHL20), as recommended by the International Society for Animal Genetics. Capillary electrophoresis was conducted using the amplified PCR products, alleles were determined. Alleles were used for statistical analysis of genetic variability, Nei’s DA genetic distance, principal coordinate analysis (PCoA), factorial corresponding analysis (FCA), and population structure.Results: On average, the number of alleles, expected heterozygosity (H_Exp), observed heterozygosity (H_Obs), and polymorphic information content among all populations were 11.43, 0.772, 0.757, and 0.737, respectively. In the PCoA and FCA, GOP, and KGP were genetically distinct from other populations, and the KTP and USP showed a close relationship. The two clusters identified using Nei’s DA genetic distance analysis and population structure highlighted the presence of structurally clear genetic separation.Conclusion: Overall, the results of this study suggest that genetic diversity between KTP and USP was low, and that between GOP and KGP was high. It is thought that these results will help in the effective preservation and improvement of Mongolian horses through genetic diversity analysis and phylogenetic relationships.

Proteomic analysis of Salmonella pullorum infected liver tissue in chicken

Urantulkhuur.B,Baatartsogt.O,H. K. Lim,S.W Suh,Y.Y Kim,C.H Lee,P.K. Mandal,K. D. Choil 한국가금학회 2008 한국가금학회 정기총회 및 학술발표회 Vol.25 No.-

Salmonellosis in poultry is a significant health problem which causes substantial economic loss. One of the common causative agents of chicken salmonellosis is Salmonella pullorum (SP). The goal of this work was to characterize and identify gene expression profiles of chicken liver tissue after infected Salmonella pullorum. In particular, this report focuses on identification of the major proteins that could be identified in Salmonella infected liver. Hundred Hy-Line layer chicks of 10 days old were divided into 5 groups of 20 each. A1, A2, A3 group were infected with high dose(3×10⁹cfu/㎖) of S.P orally and A4, A5 group were kept as non-infected control. One chick was taken randomly from each group after 0, 3, 6, 9, and 12 days and liver was collected. Proteins were separated from 6 days chicks' liver. Two-dimensional electrophoresis was done and mass spectrometry(MS) will identify the protein spots. More than 289 protein spots were detected on silver stained 2DE gels. Three new proteins were found to be expressed in the infected chicken liver. Five proteins were found to have up regulated expression in infected chicken.