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돼지편층의 45kDa 항원단백질에 대한 단클론항체 생산
이종경,김종태,서흔수,박종열,윤희정,Lee, Jong-Kyung,Kim, Jung-Tae,Seo, Hun-Su,Park, Jong-Yeol,Yun, Hee-Jeong 대한수의학회 2004 大韓獸醫學會誌 Vol.44 No.4
Trichnuris suis does not excrete eggs during larval stage as well as in particular adult stage, It is impossible to diagnose by use of fecal examination method in those periods. Therefore, serological diagnostic method can be very useful for those stages. In order to produce monoclonal antibody, specific somatic and secretory-excretory (SE) antigens of T. suis were identified and analyzed by SDS-PAGE and Western blot. Monoclonal antibody-producing hybridoma cells were cloned, which were made of popliteal lymph node of BALB/c mice immunized with a 45 kDa somatic antigen of T. suis. Five clones (1B9, 2C4, n2C5, 2D7 and 2D8) showing strong responses to T. suis antigens were selected and the isotype identified. All monoclonal antibodies were IgG1 isotype and the light chains were k chain. Established monoclonal antibodies reacted specifically to somatic and SE antigens of T. suis and did not cross-reacted to antigens of ascaris suum, trichuris vulpis, or Trichinella spiralis. The sensitivity of somatic and SE antigens against these monoclonal antibodies were significant (p<0.01) associated with those of positive and negative sera.
극영화를 통해 본 서양 역사에서의 여성 : 중등학교 세계사 수업에서의 활용 방안 연구
李鍾京(Lee Chong-Kyung),金珍?(Kim Geena),丁允珠(Jung Yoon-Joo) 歷史敎育硏究會 2009 역사교육 Vol.111 No.-
The aim of this thesis is to develop a practical and effective teaching-learning model by which students can properly understand women's experience and status in history. To achieve this aim, two lesson plans using feature films are developed. In the first lesson covering the Ancient and Medieval periods, two films of “300” and “The Messenger: Joan of Arc” are screened with focus on women’s distinct experiences. In the second lesson covering the Modern and Contemporary periods, students view three films of “Pride and Prejudice” by BBC, “Thelma and Louise” and “Something’s Gotta Give” with particular attention not to the women in general but to individual woman. The findings are as follows. First, students respond to the lessons very favorably, and want to learn more about women and women’s history. Second, students recognize that women’s experience can be different from those of men. Finally, they also come to realize that were always subjective and independent women in any time.
이종경 ( Jong Kyung Lee ) 연세대학교 간호정책연구소 2006 간호학탐구 Vol.14 No.2
King`s conceptual framework is a system theory that uses three dynamic interacting systems-personal, interpersonal, and social. King describes human as open systems interacting with the environment. King` derived the theory of goal attainment from her conceptual framework. The basic premise of the theory is the interrelationship of the nurse and the patient through action, interaction and transaction to achieve mutual goal attainment. When transaction are made between nurses and, patients, one can begin to predict that goals will be attained. The level of theory development is explanatory and predictive. In this article, King` theory was evaluated by Hardy`s evaluation criteria. That showed King`s theory was relevant in meaning and logical adequacy, testability, predictivity, contribution to understanding, pragmatic adequacy. Theory of goal attainment has been used as the basis for practice, education, research, and administration. Several middle range theories have been derived from King`s conceptual system. Growing numbers of research studies using the theory of goal attainment support the usefulness for several clinical situation, education, and administration.
이종경(Joung Kyong Lee),임근발(Keun Bal Lim),김기용(Ki Yong Kim),최기준(Gi Jun Choi),성병렬(Byung Ryul Seong),서성(Sung Seo),지희정(Hee Chung Ji),최연식(Yeon Sik Choi),신정남(Jeong Nam Shin),박형수(Hyung Soo Park) 한국초지조사료학회 2007 한국초지조사료학회지 Vol.27 No.4
본 연구는 2005년과 2006년 축산과학원 2개지역의 시험포장에서 각각 수행되었다. 1년차는 수원(중부)과 남원(남부)에서 2년차는 천안(중부)과 남원(남부)에서 수행되었으며 공시 품종은 헤어리 베치 11품종 도입품종 9품종(Penn-02, Sander, Latigo, Welta, Ostsaat, VV4712, Minnie, Barlosa 및 Capello), 국내품종 2품종(Cold green과 Cheong pa)를 이용하였다. 시험에 사용된 품종을 개화시기를 기준으로 평가한 결과 도입품종 Minnie, Barlosa, Capello 및 국내 품종 Cold green이 조생종이었고, 도입 품종 Penn-02, Sander, Latigo, Welta, Ostsaat, VV4712 및 국내품종 Cheong pa는 중만생종에 속하였다. 월동상태는 조생종 Minnie, Barlosa 및 Capello를 제외하고 대부분 우수하였다. 2년간 시험한 결과 11개 품종중 VV4712가 가장 수량이 높은 품종이었다. 그리고 중부지방에서는 중만생종의 수량이 남부지방에서는 조생종의 수량이 많았다. 두 지역에서 국내 육성품종인 중만생종 Cheong pa는 도입품종들에 비하여 뒤떨어지지 않았고 조생종 Cold green은 도입 품종들에 비하여 생육과 수량이 우수하였다. 헤어리 베치의 NDF, ADF, IVDMD 및 CP 등 사료가치는 조생종보다 중만생종이 좋았고, 그중에서 NDF는 중만생종 Latigo, ADF는 중만생종 Welta, 소화율과 조단백질 함량은 중만생종 Sander가 가장 좋았다. 이 시험의 결과 헤어리베치중 VV4712 품종의 생산성이 가장 높았고 국내 육성품종인 Cheong pa와 Cold green은 유망한 품종으로 판단된다. This experiment was conducted to compare the agronomic characteristics and productivity in introduced hairy vetch cultivars and developed new Korean hairy vetch cultivars in two areas of the experimental field of Grassland and Forage Crops Division, National Institute of Animal Science from 2005 to 2006. The experiment was arranged in randomized complete block design with three replications. A total of 11 hairy vetch cultivars used in this study including 9 introduced cultivars (Penn-02, Sander, Latigo, Welta, Ostsaat, VV4712, Minnie, Barlosa and Capello) and 2 Korean cultivars (Cold green and Cheong pa). The early flowering varieties of Minnie, Barlosa, Capello and Cold green were grown as early maturity cultivars, while the late flowering varieties of Penn-02, Sander, Latigo, Welta, Ostsaat, VV4712 and Cheong pa were grown as medium and late maturity. The winter hardiness of hairy vetch was good except for Minnie, Barlosa and Capello of early maturity cultivars. In this study, dry matter yield of VV4712 was the highest of 11 hairy vetch cultivars. And dry matter yield of medium and late maturity vetch increased in the middle region while that of early maturity increased in the south. The new Korean cultivar, Cheong pa, as a medium and late maturity hairy vetch was not bad compared to introduced cultivars and Cold green as an early maturity hairy vetch was an excellent cultivar compared to introduced cultivars in both regions. Latigo in NDF (neutral detergent fiber) and Welta in ADF (acid detergent fiber) were lower than other cultivars, while Sander in IVDMD (in vitro dry matter digestibility) and CP (crude protein) content were higher than other cultivars. The results of this experiment indicated that the productivity of hairy vetch cultivars was highest in VV4712, and Cheong pa and Cold green of new Korean cultivar were the promising cultivars of hairy vetch in Korea.