현대생물학의 방향

이민재 韓國生物敎育學會 1970 생물교육 Vol.4 No.-

微生物에 의한 石油內 硫黃分除去에 관한 硏究 : Ⅳ. 脫黃菌의 特性에 대한 生理學的 硏究 Ⅳ. Physiological Studies on Characters of Sulfur-oxidizing Bacteria

李敏載,河永七,李光雄,邊宇玄 서울대학교 1975 서울대학교 論文集 Vol.25 No.-

Serveral physiological characterstics of sulfur­oxidizing bacteria such as Thiobacillus thiooxidans and T. concretivorus are described. 1) Effect of pH on inhibitory function of pyruvate. The rate of oxygen uptake was reduced in accordance with decrease of pH. And on the other hand inhibition rate of pyruvate was more severe in lower pH than in higher pH. High concentration of hydrogen ion seemed to accelerate inhibitory function of pyruvate. Limiting concentration of pyruvate which absolutely inhibit oxygen uptake was lowered with time. In O time, 10^-2M of pyruvate absolutely inhibited oxygen uptake in pH 5. After 24 hours limiting concentration was lowered to 10^-3M. 2) Effect of organic compounds on the oxygen uptake of T. concretivorns. Effect of glucose, fructose, xylose, glutamate, succinate, malate, glycine, lactate, acetate, pyruvate, citrate, formate, and cis­aconitate on thiosulfate oxidation and availability of these compounds as sloe source of energy by T. concretivorus were observed. A 0.5% concentration of malate and glycine accelerated thiosulfate oxidation almost react somewhat inhibitory. Pyruvate and citrate inhibited thiosulfate oxidation. In thiosulfate­free medium, organic compounds except formate and pyruvate affected no significant influences on oxygen uptake. 3) Enzyme assay. Of enzymes concerned TCA cycle and glycolysis, T. concretivorus had most of those enzyme activities even though they were low. Activities of hexokinase and succinic dehydrogenase were somewhat high and that of aconitase was very low on the contrary. 4) Optimum condition of mass culture of sulfur oxidizing bacteria. In a large jar fermenter opimum conditions including agitations speed, volume of supplying gases, and constitution rate of oxygen and carbon dioxide, etc. were experimented. The sulfur­oxidier, T. thiooxidans was grown most effectively under the condition of autom­atically controlled pH of 1.0, and with the aeration of oxygen and carbon dioxide mixture (5 : 1) at the flow rate of 0.05 vvm, and at the agitation velocity of 200 rpm in this laboratory scale fermenter.

CIonidine, Methyldopa 및 Prazosin이 몇가지 昇壓物質의 作用에 미치는 影響

李玟宰,高錫太 朝鮮大學校 1987 藥學硏究誌 Vol.9 No.1

In order to investigate the effect of clonidine, methyldopa and prazosin on the pressor actions of norepinephrine, tyramine and angiotensin, this study was performed in rabbits. Clonidine, α₂-receptor agonist, markedly potentiated the pressor action of norepinephrine and slightly potentiated that of tyramine but weakened that of angiotensin. Methyldopa, L-aromatic amino-acid decarboxylase inhibitor, potentiated the pressor action of norepinephrine, whereas it did not influence that of tyramine, and weakened that angiotensin. Prazosin, α₁-receptor blocking agent, inhibited all the pressor actions of norepinephrine, tyramine and angiotensin.

음압에 의한 인체 표피의 팽창 정도 및 형태학적 변화에 관한 연구

이민재,강형철 梨花女子大學校 醫科大學 醫科學硏究所 1996 EMJ (Ewha medical journal) Vol.19 No.3

이화 여자 대학교 의과대학 부속 동대문 병원 백반증 크리닉을 방문한 백반증 환자중 표피이식술을 시행한 11명의 전흉부와 복부 18부위에서 일정한 시간동안 일정한 음압으로 흡입수포를 만들었을 때 표피 면적의 팽창 정도를 측정하고, 흡입으로 팽창된 표피의 형태학적 변화를 주사전자현미경과 투사전자현미경으로 관찰하여 다음과 같은 결과를 얻었다. 표피직경은 흡입 전 평군 7.6mm에서 흡입후 평군 11.0mm로 평균 46.4%, 표피면적은 평균 45.0㎟에서 95.9㎟으로 115.0% 증가하여, 팽창도 증가는 통계학적으로 유의하였다(p<0.05). 형태학적 변화로는 표피두께가 현저히 감소되고 표면과 평행한 방향으로 표피의 기저세포, 핵 및 세포간교가 신장된 소견을 보였으며, 각화세포 및 멜라닌세포의 핵 주변에 공포화 현상을 나타내었다. 결론적으로 표피의 팽창 정도 및 형태학적 변화 측정이 피부 표피의 물리학적 성질과 구조를 이해하는데 도움이 되며, 표피 이식수술시 필요한 정확한 공여부 피부면적을 예측하는데 이용될 수 있으리라 생각된다. Recently, autologous epidermal graft using suction-blister has been used in the treatment of depigmented diseases. The suction-blister is produced by separation of epidermis from dermis by negative pressure, and the separated epidermis is a good source for studying the properties of the epidermis itself. In this day, we measured the pre-expanded control diameter and post-expanded diameter induced by negative pressure in the anterior chest and abdomen of patients and calculated the expansibility of epidermis from the measurements, and studied the morphological changes of the expanded epidermis using scanning electron microscope and transmission electron microscope. As a result, the diameter of epidermis was elongated 46.4%, from a mean of 7.6mm to 11. 0mm and the calculated area was expanded 115.0%, from a mean of 45.0㎟ to 95.9㎟ by suction. Both were statistically significant(p<0.05). And morphologically, the thickness of epidermis was markedly decreased and the basal keratinocytes, their nuclei and intercellular bridges were elongated parallel to the skin surface, and paranuclear vacuolization was observed in keratinocytes andmelanocytes. In conclusion, above results are helpful in understanding the physical property and structure of epidermis and can be used for estimating the exact size of the donor skin needed for epidermal graft.

"植物 의 耐寒性에 關한 硏究" Ⅱ報 : 大麥類의 耐寒性과 渗透壓과의 關係에 對하여 Relative to Cold-Resistance and Osmatic Pressure with Barley

李敏載 서울대학교 1954 서울대학교 論文集 Vol.1 No.-

Taking Spring and Autumn barley which show a distinct difference on cold-resistance measuring osmotic pressure under various conditions, pursued the relation between cold-resistance and osmotic pressure. (1) Regardless the degree of cold-resistance, in the growing condition(27℃±2℃) which is adequate to temperature, no difference of osmotic pressure was observable. (2) However, whether the barley is treated under insufficient temperature after growth of grown under such condition, both Spring and Autumn barley show the adaptation to it and make the osmotic pressure raise; but the raising rate is seen higher by Autumn barley. (3) Acordingly, osmotic pressure does not become the direct cause to cold-resistance, but it becomes indirect cause through protoplam property or coll id condition. (4) The raise of osmotic pressure of the plant grown in the low temperature, does not come out by want of water supplied, but it is originated by increasing osmotic pressure caused by low temperature, the plant grown in the low temperature shows the higher osmotic pressure even in the equal water condition, But the raising rate of pressure is more higher by Autumn barley than Spring barley. (5) During the growing period, some amount of potash is applied, the more potash shows the higher osmotic pressure. (6) The barley grown in the sun-shadow shows an inclination of less osmotic pressure that of grown in the sun-light.

중소벤처기업이 알아야할 지식재산권 보호

이민재,Lee, Min-Jae 벤처기업협회 2009 벤처다이제스트 Vol.131 No.-

대한민국은 지식재산권 보유규모만을 한정하면 세계 어디에도 손색이 없는 수준의 지식재산 강국이 되었다. 그러나 세계 4위의 지식재산 보유 강국이면서도, 지식재산권의 활용이나 보호 측면에서는 여전히 많은 노력이 필요한 것이 현실이다. 최근의 지식재산권 통계자료에 의하면, 특허출원 증가율에 있어 대기업은 연평균 18.2%, 중소기업은 2.7%로 중소기업이 대기업에 비해 정체되어 있고, 더욱이 우리나라의 특허활용도는 세계 21위 수준(IMD 경쟁력보고서)이고 보면 지식재산의 보유 양에 비하여 질적 수준이 낮아 가치 창출에 미흡한 편이다. 더욱이 경기침체로 국내 대기업을 비롯한 세계 굴지의 기업들이 생존경쟁과 특허 선점을 위한 글로벌 특허전쟁을 벌이는 가운데 국내 중소벤처기업은 부족한 자금과 인력풀 속에 온갖 특허분쟁에 시달리고 있는 실정이다. 이러한 상황에서 단순히 물건을 잘 만들어 잘 판매하는 것만으로는 험난한 글로벌 특허 전쟁에서 살아남을 수 없다. 지금껏 개발하여 잘 판매하고 있는데 느닷없이 관련된 권리를 가지고 판매 및 생산을 중지시키고, 수출을 위해 선적해둔 물건을 압류해 버리는 일이 종종 발생되고 있기 때문이다. 따라서 자신의 지식재산권을 지키고 다른 이의 지식재산권을 침해하지 않도록 조심하고, 문제가 생겼을 경우 적절히 대응하는 것은 물건을 잘 만들고, 잘 판매하는 것만큼 대단히 중요하며 어쩌면 큰 수익을 낼 수 있는 투자이기도 하므로, 최근 빈번한 지식재산권 분쟁 대처사항을 점검해 보자.

Merkel 세포암 1예

이민재,임연순,조소연,강형철,함정희 梨花女子大學校 醫科大學 醫科學硏究所 1994 EMJ (Ewha medical journal) Vol.17 No.2

Merkel cell carcinoma is a rare aggressive tumor of uncertain origin. The tumor occurres most commonly in the head and neck region of the eldery. The patient was a 62-year-old female with a reddish nonulcerative movable hard nodule on her left temple area for 2 months. Histopathologically, the tumor cells with large vesicular nuclei and scanty cytoplasm were arranged in trabecular and cord-like pattern in the dermis. The majority of the tumor cells were positive for neuron specific enolase, chromogranin and neurofilament.

목장에서 채택 가능한 농가주택의 구조와 건축 절차(I)

이민재,Lee, Min-Jae 한국낙농육우협회 2007 낙농·육우 Vol.27 No.6

실험동물시설의 환경제어 기준과 평가

이민재 한국공기청정협회 2002 空氣淸淨技術 Vol.15 No.3

IMF 경제체제의 원인과 대응전략

이민재 慶尙大學校 經營行政大學院 1999 最高管理者課程論文集 Vol.16 No.-