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지렁이 중장에서 발현되는 Endo-$\beta$-1,4-glucanase의 동정 및 특성에 관한 연구
이명식,조성진,탁은식,허소영,이종애,박범준,조현주,신주옥,박순철,Lee Myung Sik,Cho Sung Jin,Tak Eun Sik,Hur So Young,Lee Jong Ae,Park Bum Joon,Cho Hyun Ju,Shin Chuog,Park Soon Cheol 한국토양동물학회 2003 한국토양동물학회지 Vol.8 No.1,2
지렁이 (Eisenia andrei)의 중장에서 내생의 endoglucanase 유전자의 전체 염기 서열을 동정하였다. ORF의 길이는 1,371 bp이며, 456개의 아미노산으로 번역된다. NCBI에 등록된 가재와 흰개미의 cellulase 및 endo-$\beta$-1, 4-glucanase와 50-51%의 유사성을 보이며, 활성 부위가 잘 보존되어 있었다. 계통수 분석에서는 다른 동물 분류군에서 밝혀진 GHF9 그룹의 cellulase와 근연관계가 없음이 확인되었다. Endogeneous endoglucanase (EC 3.2.1.4) cDNA was cloned from a representative species (Eisenia anderi) of the earthworm family Lumbricidae. Endoglucanase from the midgut of the earthworm is composed of 456 amino acids and belongs to glycosyl hydrolase family 9 (GHF9), sharing high homologies (50-51 %) with those of selected termite and crayfish. This endoglucanase consists of three consensus catalytic domains found in most microbial cellulases. A phylogenetic tree was constructed using the amino acid squence data matched through the BLASTX program and showed that GHF9 families could be divided into four groups of arthropoda, bacteria, plant and annelida.
신용카드사의 웹사이트 서비스품질이 e-로열티 및 가상공동체의식에 미치는 영향
이명식(Lee, Myung Sik) 한국서비스경영학회 2016 서비스경영학회지 Vol.17 No.2
The purpose of this study is to examine the effect of website service quality of credit card company on e-loyalty and a sense of community as well as a mediating effect of e-loyalty on relationship between website service quality and a sense of virtual community, and a moderating effect of perceived security risk on relationship between website service quality and e-loyalty. To measure website service quality, four elements are suggested as follows: communication, system operation, aesthetics, and exchange process. Five hypotheses are proposed and tested empirically. All of elements of website service quality have a positive effect on e-loyalty. Also, three element except aesthetics have a positive effect on a sense of virtual community. There are mediating effects of e-loyalty on relationship between all of elements of website service quality and a sense of virtual community, and moderating effects on both relationship between system operation and e-loyalty and relationship between exchange process and e-loyalty. Further theoretical and managerial implications as well as research limitations are discussed.
이명식(Lee Myung-Sik) 대한건축학회 2009 대한건축학회논문집 Vol.25 No.2
People are getting information for building through various medium. But people can not approach the required data in spite of necessity and heft of performance information of building grade. Because synthetic evaluation model and systematic computing system for performance information of building is not consisting in domestic circumstance. That is why this paper is intended to propose computer based implementation model for Building Performance Evaluation. This research suggests (1)the criteria of performance evaluation items to be divided into two categories - performance of building itself, performance of exterior effect on building (2)the implementation scheme of web-based building performance evaluation system through computer aided model and the information classification system for the building performance evaluation.