Effects of MNNG and UV-light on Chromosome Aberration and Excision Repair

이천복 慶星大學校 1986 부산수산대학 논문집 Vol.7 No.2

본 연구는 서로 다른 상해요인인 자외선과 MNNG의 복합처리가 DNA 절제회복 및 염색체 이상에 미치는 영향과 이드에 의하여 유발된 DNA 회복합성과 염색체 이상과의 연관성 여부를 규명할 목적으로 수행하였다. 그 결과는 다음과 같다. (1) DNA 회복합성은 자외선과 MNNG를 단독 처리한 군에서는 조사선량 및 농도에 따라 증가 하였으며, 자외선과 MNNG를 복합처리한 군에서는 이들의 단독 처리군의 합보다 작았으며 자외선 단독처리군 보다도 작았다. 이는 MNNG가 자외선에 의하여 유발된 DNA 회복 합성을 억제하는 것을 암시하고 있다. (2) 자외선과 MNNG에 의하여 상해된 DNA 회복 합성율은 처리직후 최대를 보이며 시간이 경과함에 따라 점차 감소하나 24시간 후에도 높은율을 나타내고 있다, 이는 자외선과 MNNG에 의한 상해가 오래 지속됨을 시사하는 것이다. (3) 자외선과 MNNG를 복합처리한 군에서의 염색체 이상률은 자외선과 MNNG 단독처리군의 합보다 높았다. (4) 시간 경과에 따른 DNA 회복 합성과 염색분체 절단, 염색분체 교환과는 어떠한 연관도 보이지 않았다. 이는 자외선과 MNNG에 의한 DNA 회복합성과 염색체 이상과는 연관성이 없음을 시사하는 것이다. The present investigation has been performed to determine how different pairs of DNA damaging agent, interact in inducing excision repair and chromosome aberration, and to determine the possible relationship between the excision repair and chromosome aberrations in CHO cells, The results obtains were as follows: (1) Unscheduled DNA synthesis induced by UV-light and MNNG showed dose dependent increase. However, the amount of unscheduled DNA synthesis by the combined treatment with UV-light and MNNG was found to be much less than the sum of those in single treated group, and even lower than that produced by UV-light alone. These results indicate that MNNG inhibits the UV-induced unscheduled DNA synthesis. (2) The maximum rate of DNA repair synthesis was occured in the CHO cells soon after the treatment. The excision repair was then gradually decreased with time and detected at 24 hours after treatment with high frequency. This result indicates that the DNA damage induced by MNNG and UV-light is long lasting damage to be repaired. (3) The aberration rate of combined treatment is higher than the sum of the rates produced by UV-light and MNNG seperately. These results indicate that combined treatment with MNNG and UV-light and MNNG seperately. (4) The rates of excision repair do not show a correlation with either deletions or exchanges in each time group. These results may suggest that excision repair may not be related to chromosome aberrations in MNNG and UV-light treated CHO cells.

염색체 이상과 자매염색분체교환에 미치는 anti-calmodulin drug의 영향

이천복 慶星大學校 1999 論文集 Vol.20 No.1

The present study was performed to elucidate the effects of TFP and W-13(anti calmodulin drug) on chromosome aberrations and sister chromatid exchanges induced by MNNG. The results obtained were as follows: 1) Both the chromosome aberrations and sister chromatid exchanges were not induced by the treatment of various concentration and times of TFP or W-13. 2) TFP and W-13 inhibited chromosome aberrations induced by MNNG regardless of the concentration and times of treatment. 3) In the groups treated with 5 M TFP or 10 M W-13 for 1 hour after treatment of MNNG, sister chromatid exchanges induced by MNNG were little effected. However, in the groups treated with more than 10 M TFP or 20 M W-13, sister chromatid exchanges induced by MNNG increased with TFP OR W-13 treatment unlike chromosome aberrations. 4) TFP or W-13 treatment for 36 hours after treatment of MNNG increased the sister chromatid exchanges induced by MNNG. The aspects of increase were proportional to the concentration of TFP and W-13 treatment. These data indicate that TFP or W-13 itself dose not induce chromosome aberrations and sister chromatid exchanges, but is concerned in chromosome aberrations and sister chromatid exchanges induced by MNNG; that is, it has the ability to decrease chromosome aberrations but to increase sister chromatid exchanges.

Chinese Hamster Ovary세포에 있어 Methyl Methanesulfonate에 의한 DNA복제억제와 이의 회복경로

이천복,이형호,이치건,박상대 한국환경성돌연변이발암원학회 1989 한국환경성돌연변이·발암원학회지 Vol.9 No.1

Effects of MNNG on Ultraviolet Light induced sister chrometid Exchanges.

이천복 경성대학교 1985 부산수산대학 논문집 Vol.6 No.2

자매염색분체 교환에 미치는 자외선과 MNNG의 상호작용을 규명하기 위하여 CHO세포를 재료로 자외선과 MNNG를 복잡처리한 후 자매염색분체 교환율을 조사하여 다음과 같은 결론을 얻었다. (1) 자외선 단독 처리군에서의 자매염색분체 교환은 자외선의 선량 증가에 비례하여 증가하였다. (2) MNNG 단독처리군에서도 MNNG 농도증가에 비례하여 자매염색분체 교환율이 증가하였다. (3) 자외선과 MMNG를 복합처리한 군에서의 자매염색분체 교환율은 자외선과 MNNG를 단독처리한 것의 합보다 작았으며 0.01μM MNNG를 복합처리한 군의 경우 자외선 단독처리군보다 오히려 작았다. 이상의 결과는 MNNG가 자외선에 의하여 유발된 자매염색분체 교환을 방해하는 것을 보여주고 있다. The present investigation has been undertaken to determine the interaction between UV-light and MNNG, in inducing SCEs. (1) In UV-light irradiated groups, the rates of SCEs were sharply increased with increasing dose. (2) MNNG induces SCEs in dose-dependent manner. (3) Induction of SCEs exposed to UV-light and MNNG were less than that from the sum of each agent sperately and even lower that produced by single UV-light irradiation (0.01μM MNNG). The present results showed that MNNG interfered with the molecular machinery involved in UV-light induced SCE formation, and indicated that there is a considerable overlap in the mechanisms that regulate the total amount of SCEs performed for each of these agents.

지질개선(地質改善) 방법중(方法中) 압력(壓力) 그라우팅에 관(關)하여

이천복 한국농공학회 1965 韓國農工學會誌 : 전원과 자원 Vol.7 No.2

비타민C가 돌연변이원에 의하여 유발되는 염색체 이상에 미치는 영향

이천복 慶星大學校 1998 論文集 Vol.19 No.1

This study was performed to investigate the effects of vitamin C on the chromosome aberrations caused by UV light and MNNG which were known for a strong mutagen. The results obtained were as follows; 1) Treatment of vitamin C for 48 hours did not affect chromosome aberrations of control group. 2) Chromosome aberrations of vitamin C-pretreated group were decreased compared with those of MNNG-treated alone group. 3) Chromosome aberrations induced by simultaneous treatment or posttreatment of vitamin C and MNNG showed similar levels to those induced by treatment of MNNG alone. 4) When vitamin C was treated before exposure to uv light chromosome aberrations were decreased. 5) Chromosome aberrations were slightly decreased in cases of post-treatment with vitamin C after exposure to uv light. These results suggest that vitamin C may not have a mutagenic effect when it was treated for 48 hours, it has an antimutagenic effect on that are induced by MNNG and uv light, and antimutagenic effect of pretreatment of vitamin C is stronger than that of simultaneous treatment or posttreatment.

수산화인회석 배합치약 잇솔질이 인체영구치 표면에 미치는 영향에 관한 연구보고

이천복,최유진 ( Chon Bok Lee,Eu Gene Choi ) 대한예방치과·구강보건학회 1997 大韓口腔保健學會誌 Vol.21 No.4

Colloidal Silicone Dioxide와 Aluminum Hydroxide가 간이구강위생지수와 치은지수에 미치는 영향에 관한 연구

이천복,최유진 慶熙大學校 齒科大學 1995 慶熙齒大論文集 Vol.17 No.1

This clinical study was performed for the purpose of investigating the effect of gingival index and simplified oral hygiene index of two dentifrices containing different abrasive agents. 40 dental collage students of age 2 "i-26 were divided 20 members of control group with using dentifrices containing colloidal silicone dioxide(SiO?), and others as experimental group with using dentifrices containing aluminum hydroxide(Al(OH)3) as abrasives. The gingival index and simplified oral hygiene index were analyzed before the experimental and the control dentifrices were supplied and there after 6 weeks. The results were as follows 1. Mean SOW was reduced 20.7% by control group and 31.6% by experimental group stastistical difference was observed: 2. Mean GI was reduced 49.1% by control group and 55.0% by expenmental dentifrices group, but significant difference was not observed. 3. Significant difference in GI and SOW by tooth brushing method was not observed. 4. Factual knowledge about the abrasives, brushing method and pharmacy is scarce and further research on relevant topics seems to be indicated.

고등학교 생물 I 교과서에 수록되어 있는 실험 . 관찰 방법의 적합성 조사

강미애,이천복 慶星大學校 1998 論文集 Vol.19 No.2

This study was performed to point some problems that teachers meet when they teach the experiments with high school biology I textbooks and also to suggest the solution. The results are as follow. Because of the lack of the detailed explanations on the 'method' (for example, the method of making the reagent and its concentration, the magnifying scale of the microscope), it is difficult for the teacher to guide the students to get the exact results. And the lack of diagrams, figures, and pictures with which the results are to be compared makes it difficult to judge the credibility of the result. Furthermore for a number of the experiment, it is not possible to get the results within 50 minutes. It is desirable to replace them with the plausible ones.

한발(旱魃)에 대비(對備)한 지하수개발(地下水開發)의 제언(提言)

이천복 한국농공학회 1981 韓國農工學會誌 : 전원과 자원 Vol.23 No.2