사구체 상피세포와 혈관사이세포 내에 안지오텐신 II와 레닌-안지오텐신계 구성인자들의 공존

이원정,고철우,박매자 대한해부학회 2002 Anatomy & Cell Biology Vol.35 No.2

Differences in Proportion of N-acetyllactosamine and O-acetylated Sialic Acid Have No Significant Effect on the Pharmacokinetics and Biological Activity of Darbepoetin Alfa

이원정,오후근,최인성,이근호,신현우,이윤정,박지혜,양유희,하경식,이동억,최강열,최은영 한국생물공학회 2022 Biotechnology and Bioprocess Engineering Vol.27 No.2

Darbepoetin alfa is used to treat anemia in patients with chronic renal failure and is a therapeutic glycoprotein with five N-glycosylation sites and one Oglycosylation site. Since the glycosylation of therapeutic proteins is known to affect pharmacokinetics and biological activity, it was wondered how the certain glycosylation structure of darbepoetin alfa affects pharmacokinetics and biological activity. To investigate the effects of glycosylation structures, several darbepoetin alfa samples were generated through the processes slightly modified from HK-DPO production process. Analysis of N-linked glycosylation profiles showed that darbepoetin alfa samples have different proportions of N-acetyllactosamine and O-acetylated sialic acids. Since these structures were known to affect the stability and efficacy of glycoproteins, we evaluated the pharmacokinetics and biological activities of darbepoetin alfa samples. As a result of pharmacokinetics, the time course of the concentration in plasma was similar to that of NESP®, commercially available darbepoetin alfa. Also, the relative AUClast ratio (sample/reference) was ranged from 84% to 114%, which indicated they did not have meaningful differences. For in vitro biological activity, a cell proliferation test was conducted in the EPO-dependent F36E cell-line. An in vivo biological activity test was performed with EP-based assay using B6D2F1 female mice. As a result, the biological activities of each samples were similar to those of reference drug, NESP®. In conclusion, different proportions of N-acetyllactosamine and O-acetylation of sialic acid in darbepoetin alfa did not show any meaningful effects on pharmacokinetics and biological activities.

평택 칠원동 지구단위계획부지(3-2단계) 내 유적

이원정 중부고고학회 2019 중부고고학회 학술대회논문집 Vol.2019 No.3

다중 퍼셉트론 기법을 활용한 철도차량 견인용 영구자석 동기 전동기의 영구자석 형상 설계 유효성 검증

이원정,이재범,이형우,임재현,김충서,홍석민,이찬규,백종민,박찬배 한국철도학회 2023 한국철도학회 학술발표대회논문집 Vol.2023 No.11

치료 방사선량이 쥐의 난포 퇴축에 미치는 영향

이원정,선종률,유세종,안봉선 대한방사선방어학회 2012 방사선방어학회지 Vol.37 No.4

반선지연으로 인한 손해배상액의 산정 - The Achilleas사건에서 영국 최고법원 판결을 중심으로

이원정 해상보험법연구회 2010 해상보험법연구 Vol.6 No.-

분진에 노출된 집단에서 관상동맥 석회화와 전통적인 심혈관질환 위험 평가의 관계

이원정,박소영 대한영상의학회 2013 대한영상의학회지 Vol.68 No.5

Purpose: The purpose of this study is to determine the relationship between the coronary artery calcification (CAC) and the conventional cardiovascular disease risk assessment (CCDRA). Materials and Methods: This study included 101 subjects who were exposed to inorganic dusts and underwent CAC scoring by multidetector CT (MDCT), laboratory tests, and a standardized questionnaire for CCDRA, after being approved from the Institutional Review Board and providing informed consent. All subjects were divided as either non-calcified group (< 1, 55.4%) or calcified group (≥ 1, 44.6%) from total CAC, and evaluated by CCDRA, such as Framingham risk score (FRS) and National Cholesterol Education Program (NCEP). Receiver operating characteristic (ROC) area of FRS was generated for predicting CAC risk using SPSS program (ver. 19.0, Chicago, IL, USA). Results: Total CAC was significantly correlated with FRS (r = 0.283, p = 0.004). Crude odds ratio for CAC risk was 3.64 [95% confidence interval (CI) 1.39-9.52] in FRS ≥ 20%, and 2.87 (95% CI 1.24-6.65) in the high risk group of NCEP. Subjects with pneumoconiosis showed higher values of CAC (p = 0.541) and FRS (p = 0.035) scores compared with subjects without pneumoconiosis. ROC area of FRS was 0.69 (95% CI 0.59-0.79) with a cutoff point of 13.5%. Conclusion: CAC measured MDCT is significantly correlated with FRS than other CCDRA. 목적: 본 연구는 관상동맥 석회화와 전통적인 심혈관질환 위험 평가의 관계를 알아보고자 하였다. 대상과 방법: 다중검출기 CT (multidetector CT; MDCT) 관상동맥 석회화 검사, 혈액학적 검사와 심혈관질환의 위험요인에 대해 설문조사를 실시한 분진 노출자 101명의 자료를 이용하였다. 모든 검사는 기관연구윤리심의위원회 승인과 연구 참여 동의서를 받은 후 실시하였다. 연구 대상자를 관상동맥 총 석회화로부터 비석회화군(< 1, 55.4%)과 석회화군(≥ 1, 44.6%)으로 분류하였고, Framingham risk score (FRS)와 National Cholesterol Education Program (NCEP)으로 심혈관질환 위험을 평가하였다. SPSS 19.0 프로그램을 사용하여 관상동맥 석회화 위험에 대한 FRS의 절단점을 구하기 위해 receiver operating characteristic (ROC) 분석을 하였다. 결과: 관상동맥 석회화군의 나이, FRS와 NCEP 점수, 고혈압자가 비석회화군보다 유의하게 높았고(p < 0.05), 관상동맥 총 석회화 수치는 FRS와 유의한 상관성을 보였다(r = 0.283, p = 0.004). 관상동맥 석회화 위험은 FRS ≥ 20% 군이 미만군보다 3.64배[95% confidence interval (CI) 1.39~9.52], NCEP에서는 심혈관질환 고위험군이 저위험군보다 2.87배(95% CI 1.24~6.65) 높았다. 관상동맥 석회화와 FRS의 위험이 증가할수록 진폐증자도 증가하였다. 관상동맥 석회화 위험에 대한 FRS의 ROC 면적은 0.69(95% CI 0.59~0.79), 절단점은 13.5%였다. 결론: 전통적인 심혈관질환 위험 평가 중에서 FRS가 MDCT로부터 측정된 관상동맥 석회화와 가장 높은 관련성을 보였다.

덩굴쪼김병 저항성 멜론을 위한 효율적이고 간편한 대량 검정법 개발

이원정,장경수,최용호,김흥태,김진철,최경자 한국식물병리학회 2015 식물병연구 Vol.21 No.3

This study was conducted to establish a simple mass-screening method for resistant melon to Fusarium wilt caused by Fusarium oxysporum f. sp. melonis (FOM). Root-dipping inoculation method has been used to investigate resistance of melon plants to Fusarium wilt. However, the inoculation method requires a lot of labor and time because of complicate procedure. To develop a simple screening method on melon Fusarium wilt, occurrence of Fusarium wilt on susceptible and resistant cultivars of melon according to inoculation method including root-dipping, soil-drenching, tip, and scalpel methods was investigated. Scalpel and tip methods showed more clear resistant and susceptible responses in the melon cultivars than root-dipping inoculation method, but tip method represented slightly variable disease severity. In contrast, in the case of soil-drenching inoculation method, disease severity of the susceptible cultivars was very low. Thus we selected scalpel method as inoculation method of a simple screening method for melon Fusarium wilt. By using the scalpel inoculation method, resistance degrees of the cultivars according to incubation temperature after inoculation (25 and 30oC) and inoculum concentration (1×106 and 1×107 conidia/ml) were measured. The resistance or susceptibility of the cultivars was hardly affected by all the tested conditions. To look into the effectiveness of scalpel inoculation methods, resistance of 22 commercial melon cultivars to FOM was compare with root-dipping inoculation method. When the melon cultivars were inoculated by scalpel method, resistance responses of all the tested cultivars were clearly distinguished as by root-dipping method. Taken together, we suggest that an efficient simple mass-screening method for resistant melon plant to Fusarium wilt is to sow the seeds of melon in a pot (70 ml of soil) and to grow the seedlings in a greenhouse (25±5oC) for 7 days, to cut the root of seedlings with a scalpel and then pour a 10 ml-aliquot of the spore suspension of 1×106 conidia/ml on soil. The infected plants were cultivated in a growth room at 25 to 30oC for about 3 weeks with 12-hr light a day. 본 연구는 Fusarium oxysporum f. sp. melonis에 의해 발생하는 멜론 덩굴쪼김병의 간편 대량 저항성 검정법을 확립하기 위하여 수행하였다. 멜론의 덩굴쪼김병 저항성 검정에는 대부분뿌리 침지(root-dipping) 접종 방법을 사용하고 있지만, 이 방법은 접종과정이 번거로워 노동력과 시간이 많이 소요된다. 간편저항성 검정법을 개발하기 위해 뿌리 침지, scalpel, tip 및 토양관주 방법으로 F. oxysporum f. sp. melonis 균주를 감수성 및 저항성 멜론 품종에 접종하여 덩굴쪼김병 발생을 조사하였다. 그결과 멜론 품종들은 scalpel 방법과 tip 방법에서 뿌리 침지 방법에서와 같은 분명한 저항성과 감수성 반응을 보였다. 하지만tip 방법은 scalpel 방법보다 개체간의 발병도 차이가 약간 더심하였으며 토양 관주 방법의 경우에는 감수성 멜론 품종들에서 덩굴쪼김병 발생이 매우 낮았다. 따라서 멜론 덩굴쪼김병의간편 대량 저항성 검정을 위한 효율적인 접종 방법으로 scalpel 방법을 선발하였다. 그리고 scalpel 방법으로 접종할 때 접종 농도(1×106, 1×107 conidia/ml)와 접종 후 재배 온도(25, 30oC) 에 따른 멜론 품종들의 덩굴쪼김병 발생을 조사한 결과, 이들은 scalpel 방법으로 접종된 멜론 품종의 저항성 정도에 거의 영향을 미치지 않았다. 그리고 확립한 간편 검정법을 이용하여 시판 멜론 22개 품종의 덩굴쪼김병 저항성을 뿌리 침지 접종 방법과 비교하여 실험하여 이 방법의 효용성을 확인하였다. 이와 같은 결과로부터 멜론 덩굴쪼김병에 대한 간편 대량 저항성 검정법으로 멜론 종자를 파종하고 온실(25±5oC)에서 7일 동안 재배한 유묘의 뿌리를 scalpel을 이용하여 상처를 준 후에 1×106 conidia/ml 농도의 멜론 덩굴쪼김병균 포자현탁액을 포트 당10 ml씩 관주하고 25–30oC에서 약 3주일 동안 재배하는 것을제안하고자 한다.

환경보건적 요소가 도시 내 폭염 취약성 평가 결과에 미치는 영향 분석

이원정,강재은,김유근,Lee, Won-Jung,Kang, Jae-Eun,Kim, Yoo-Keun 한국환경보건학회 2013 한국환경보건학회지 Vol.39 No.6

Objectives: This analysis seeks to evaluate the impact of environmental health factors (EHF; e.g. hospital beds per capita, employees of medical institutions) on extreme-heat vulnerability assessment in Busan Metropolitan City during 2006-2010. Methods: According to the vulnerability concept suggested by the Intergovernmental Panel on Climate Change (IPCC), extreme-heat vulnerability is comprised of the categories of Exposure, Sensitivity, and Adaptive Capacity (including EHF). The indexes of the Exposure and Sensitivity categories indicate positive effects, while the Adaptive capacity index indicates a negative effect on extreme-heat vulnerability. Variables of each category were standardized by the re-scaling method, and then each regional relative vulnerability was computed with the vulnerability index calculation formula. Results: The extreme-heat vulnerability index (EVI) excepting EHF was much higher in urban areas than in suburban areas within the metropolitan area. When EHF was considered, the difference in the EVI between the two areas was reduced due to the increase of the Adaptive capacity index in urban areas. The low EVI in suburban areas was induced by a dominant effect of natural environmental factors (e.g. green area) within the Adaptive capacity category. Conclusions: To reduce the vulnerability to extreme heat in urban areas, which were more frequently exposed to extreme heat than others areas, public health and natural environments need to be improved in sensitive areas.

CIP조건의 국제물품매매계약에서 항공화물에 대한 피보험이익 - 대법원 2018.3.15. 선고 2017다240496판결을 중심으로 -

이원정 한국해법학회 2020 韓國海法學會誌 Vol.42 No.2

CIP조건상 매도인은 합의된 장소에서 자신이 지명한 운송인에게 물품을 인도하고, 지정목적지까지 물품의 이동을 위한 운송계약을 체결해야 하며, 특히 운송 중 물품의 멸실 또는 손상에 대한 매수인의 위험을 담보하는 보험계약을 체결해야 한다. 그런데 보험계약상 보험자로부터 보상이 이루어지기 위해서는 피보험자가 손해발생시점에서 보험목적물에 대하여 피보험이익을 가져야만 한다. 2018년 우리나라 대법원의 「2017다240496판결」에서는, CIP조건상 매도인이 매수인의 위험부담 구간인 항공운송 중에 발생된 물품의 손해에 대하여 피보험자로서 보험금을 청구할 수 있는지가 쟁점이 되었다. 대법원은 매도인이 보험자로부터 보상을 받을 수 있는 적하보험증권상 피보험자이며, 물품이 목적지에 손상 없이 도착할 때까지 물품의 소유자이자 기대이익을 갖는 자로서 피보험이익을 갖는다고 판시하였다. 이번 판결은 CIP조건에 따라 매수인을 위해 매도인이 체결한 항공적하보험계약상 매도인의 법적 지위에 관한 문제를 최초로 다루었다는 점에서 중요한 의미가 있다. 따라서 본 논문은 대법원의 판결이유를 분석하고, 상법상 타인을 위한 보험의 법리, 협회적하약관(항공)의 관련규정, CIP조건의 법적 성질, 항공화물운송장의 발행 관행 등을 기초로 판결의 타당성을 평가한다. Under CIP Term, the seller delivers the goods to the carrier nominated by him at an agreed place, and must contract for carriage necessary to bring the goods to the named place of destination, and also should contract for insurance cover against the buyer’s risks of loss of or damage to the goods during the carriage. It should be noted that under the insurance contract, in order to recover from the insurer, the assured must have an insurable interest in the subject-matter insured at the time of loss. In 2018, a decision(2017Da240496) as to whether the seller as the assured, can claim insurance money against the insurer for loss of goods during air transit which is the buyer’s risk was handed down by the Korean Supreme Court. The Supreme Court decided that the seller was the assured under cargo insurance policy to recover loss of goods from the insurer, and had also an insurable interest as the owner of the goods as well as the holder of expected profit, until the goods arrived at the destination safely. This case has important implication to academic world as well as business world, because it was the first case in Korea which handled the issue as to the seller’s legal position under the contract for air cargo insurance. Therefore, this article attempts to analyze the reasoning behind the Supreme Court's decision, and evaluates the appropriateness of the court's decision on the basis of the legal principle on insurance for benefits of third party under Korean Commercial Act, relevant provisions of Institute Cargo Clause(Air), legal nature of CIP term, practice of Air waybill’s issuance.