집토끼의 갑상선내 Parafollicular Cells에 대한 전자현미경적 연구

고정식,양남길 순천향대학교 1981 논문집 Vol.4 No.2

In order to study the fine structure of the parafollicular cells in the rabbit thyroid gland, small b1ocks of the thyroid tissue were fixed in the mixture of 2% paraformaldehyde-2.5 % glutara1dehyde (phosphate buffer, pH 7.4) and post-fixed in 1 % osmium tetroxide(phosphate buffer, pH 7.4). The tissue were embedded in Epon 812, ultrathin-sectioned with the LKB-Ⅲ ultramicrotome. The sections contrasted with uranyl acetate and lead citrate were observed with a JEM 100B electron microscope. Observed results were as follow: 1. Of the rabbit parafollicular cells. the cells with electron-lucent cytoplasm (light cell) and with electron-dense cytoplasm (dark cell) can be distinguished. 2. The parafollicular cells of the rabbit were observed in the intra-. para-and inter-follicular positions. Inter-follicular cells occur as groups of a few cello. None of intra-follicular cells have contact with the follicular lumen. 3. The parafollicular cells have oval or round indented nuclei. They contain moderately developed cell organelles. such as Golgi complex, granular doplasmic reticulum, ribosomes, etc. 4. The membrane-bound granules are spherical with a diameter of 150∼380nm. The electron densities of the core texture are variable from the coarse granule with lower density to the homogeneous granule with higher dentsity.

연령에 따른 흰쥐 신사구체의 변화에 대한 전자현미경적 연구

양남길,한종원,안의태,고정식,박경호,Yang, Nam-Gil,Han, Jong-Won,Ahn, Eui-Tae,Ko, Jeong-Sik,Park, Kyung-Ho 한국현미경학회 1987 Applied microscopy Vol.17 No.1

Morphological difference of the renal glomerulus at different age groups have been studied in young (three month-old), adult (twelve month-old) and old (thirty month-old) Fisher strain 344 rats. Pieces of the tissues were taken from renal corticies prefixed with 2.5% glutaraldehyde-1.5% paraformaldehyde (0.1 M Millonig's phosphate buffer, pH 7.3), following by post-fixation with 1% osmium tetroxide (0.1 M Millonig's phosphate buffer, pH 7.3) and embedded within Araldite. The ultrathin sections contrasted with uranyl acetate and lead citrate were observed under a JEM 100CX electron microscope. The mean thickness of glomerular basal lamina and Bowman's capsule were determined by measuring the thinnest portion of basal lamina, and by taking the average of 50 readings from electron micrographs at different ages. The numerical changes of the slit pores were compared based upon the numbers over the length of 10um of glomerular basal lamina. The results were as follow: 1. The thickness of glomerular basal lamina is increased during aging; 140.4 nm in young rats, 270.0 nm in adult ones, and 437.8 nm in old ones. 2. The thickness of basal lamina of parietal cells of Bowman's capsule is 187.5 nm in young rats, 914.0 nm in adult ones, and 2850.0 nm in old ones. 3. The numbers of the slit pores of basal lamina are reduced during aging, 30.3 slit pores/$10{\mu}m$ in adult ones, and 24.2 slit pores/$10{\mu}m$ in old ones. 4. Accumulation of dense intracytoplasmic filamentous material in the parietal cells of Bowman's capsule is increased in the vicinity of the basal lamina during aging. The proximal tubule-like epithelial cell in Bowman's capsule is observed at one glomerulus in a young rat. 5. The endothelial cells are edematous and form balloon-like structure protruding into capillary lumen in young and old rats. 6. Cytoplasm of the podocyte shows a variety of alteration during aging, such as swelling of mitochondria and of endoplasmic reticulum, and increase of microtubules, microfilaments, lysosomes and lamellated myelin structures, etc. Accumulation of dense intracytoplasmic material in the foot processes is increased in the vicinity of the basal lamina during aging. The podocytic membrane-like structures are seen in young and o]d rats. 7. The mesangial matrices and mesangial cells are increased during aging, and slight swelling of endoplasmic reticulum and Golgi cisternae in young and old rats.

韓國人蟲樣筋의 變異에 關하여

梁南吉,崔月鳳 醫藥係社 1965 綜合 醫學 Vol.10 No.5

紅花 Extract가 家兎 血淸脂質 및 脂蛋白分劃에 미치는 影響

양남길,고광삼,차종희 朝鮮大學校 醫學硏究所 1979 The Medical Journal of Chosun University Vol.- No.-

In this study, the influence of carthami flos extract (CFE) on the serum levels of total cholesterol, triglyceride, phosphohpid and lipoprotein fraction patterns in rabbits. Carchami flos was extracted by water. and the extract was orally administeied to rabb- its that was starvated for three days. The results were as follows ; 1. By administration of CFE, the strum levels of total cholesterol and triglvceride were gradually decreased and were returned to about the control levels after 15 days. 2. The serum phospholipid level was slightly increased to the control level by adminis-tration of CFE. 3 The serum α-lipoprotein fraction was decreased, pre-β-lipoprotein fraction was incr-eased and β-lipoprotein fraction was gradually decreased after 15-days by administration of CFE. 4. After three days starvation, the serum levels of total cholesterol. triglyceride were markedly increased, while phospholiped level was slightly decreased in rabbits.

X-선 조사를 받은 흰쥐 귀밑샘 샘포의 미세구조

양남길,박인규,안의태,고정식,박경호,김진국,Yang, Nam-Gil,Park, In-Kyu,Ahn, E-Tay,Ko, Jeong-Sik,Park, Kyung-Ho,Kim, Jin-Gook 한국현미경학회 1994 Applied microscopy Vol.24 No.1

Xerostomia and xerophthalmia are delicate or serous side effects, occuring when the radiotherapy is administered to the head and neck cancer patient. It is known that the cause of the above side effect is radiosensitivity of serous cells. In this study, the ultrastructural features of the parotid glands of the X-irradiated rats were observed. Sprague-Dawley rats weighing 200-250g each were anesthetized with sodium thiopental, and placed on the Mitsubishi linear accelerator. Only the head and neck areas of animals were exposured at the distance of 80cm, within the area of $30X30cm$, in the depth of 1cm, with the speed of 200R/min. Total doses applied were 3,000R or 6,000R depending on the experimental groups. Animals were sacrificed on the 6th hour, 2nd day and 6th day after the irradiation. Parotid glands were fixed in the 2.5% glutaraldehyde-1.5% paraformaldehyde solution, and followed by refixation in the 1% osmium tetroxide solution. Dehydrated blocks were embedded in araldite mixture, and ultrathin sections were cut. Sections were contrasted with the solution of uranyl acetate and lead citrate, and observed with JEM 100 CX-II electron microscope. The results were as follows: 1. Normal parotid acinar cells are two types; the light and the dark acinar cells. The light acinar cell contains dense secretory granules, whereas dark acinar cells contains granules of medium density with some darker spots within them, or other cells contain granules of medium density with darker rims. 2. Six hours after the irradiation, many acinar cells were degenerated showing variable stages of cytolytic bodies, light bodies, or dense degenerations. Within the acinar cell, Golgi apparatus and granular endoplasmic reticula were most severely altered elements. Granules showed more contrasting densities and irregularities. 3. Two days after the irradiation, some cytolytic bodies, and focal lucent degeneration of cytoplasm, and fine granular alteration of cytoplasmic matrix were pronounced. But other elements including secretory granules are rather looked unlatered. 4. Six days after the irradiation, most severe alterations were seen. Many intracellular canaliculi (or secretion figures), quanta of cytoplasm containing secretion antecedants, severely irregular luminal border, and again contrasting density of secretory granules showing tigroid spots or dense rims were noted. And myoepithelial degenerations were observed not uncommonly. 5. Irregular densities of secretory granules were interpreted as abnormal components of protein or carbohydrate portion are synthesized or abnormally metabolized under severe X-irradiation. 6. Myoepithelial degeneration and related alteration of nerve endings, etc., were suggested as the other causes of xerostomia following X-irradiation. 7. It is requested that radiation doses should be arranged, considering in mind not only the sensitivity of acinar cells but also the myoepithelial and neural functions.

韓國人胎兒의 內臟重量에 關한 硏究

梁南吉 서울의대 1965 서울의대잡지 Vol.6 No.1

부신절제후의 위점막 점액세포의 미세구조

양남길,구광일,안의태,고정식,박경호 順天鄕大學 1989 순천향대학논문집 Vol.12 No.3

손상받은고양이 줄무늬체에서 축삭반응에 관한 미세구조적 연구

양남길,조영열,안의태,고정식,박경호,김진국 대한해부학회 1994 Anatomy & Cell Biology Vol.27 No.3

韓國人 薦骨의 性差와 性別에 關한 硏究

梁南吉 醫藥係社 1964 綜合 醫學 Vol.9 No.2

紅花 Extract가 家兎 血淸脂質 및 脂蛋白分劃에 미치는 影響

양남길,고광삼,차종희 順天鄕大學校 1979 의대논문집 Vol.4 No.-

In this study, the influence of carthami flos extract (CFE) on the serum levels of total cholesterol, triglyceride, phospholipid and lipoprotein fraction patterns in rabbits. Carthami flos was extracted by water, and the extract was orally administered to rabbits that was starvated for three days. The results were as follows ; 1. By administration of CFE, the serum levels of total cholesterol and triglyceride were gradually decreased and were returned to about the control levels after 15 days. 2. The serum phospholipid level was slightly increased to the control level by administration of CFE. 3. The serum α-lipoprotein fraction was decreased, pre-β-lipoprotein fraction was increased and β-lipoprotein fraction was gradually decreased after 15-days by administration of CFE. 4. After three days starvation, the serum levels of total cholesterol, triglyceride were markedly increased, while phospholipid level was slightly decreased in rabbits.